EIF2B4

General Information

Gene Name       : EIF2B4
Gene ID             : ENSG00000115211.15
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: EIF2B4)


Antibody Validation

 View validation images for all EIF2B4 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878EIF2B4ENSG00000115211.1510.1218.5214.32ENCSR000AEF
HepG2EIF2B4ENSG00000115211.15109.9584.7397.34ENCSR985KAT
K562EIF2B4ENSG00000115211.1549.1553.9851.56ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
EIF2B4A302-982A1BGKcLV38-41, Submitted, ENCORE2
EIF2B4A302-983A1BGHcLV39-49, Submitted, ENCORE2BGKcLV38-41, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
EIF2B4K562CRISPREIF2B4-BGKcLV38-41,EIF2B4-BGKcLV38-42NT-BGKcLV38-1,NT-BGKcLV38-258.1,52.3SubmittingENCORE2
EIF2B4HepG2CRISPREIF2B4-BGHcLV39-49,EIF2B4-BGHcLV39-50NT-BGHcLV39-1,NT-BGHcLV39-267.1,76.6SubmittingENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2B4Product_ID: A302-982A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF2B4-human		 EIF2B4-K562-CRISPR-A302-982A.png<br>Caption: Western blot following CRISPR against EIF2B4 in K562 whole cell lysate using EIF2B4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2B4. EIF2B4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
EIF2B4Product_ID: A302-983A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF2B4-human		 EIF2B4-HEPG2-CRISPR-A302-983A.png<br>Caption: Western blot following CRISPR against EIF2B4 in HepG2 whole cell lysate using EIF2B4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2B4. EIF2B4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 EIF2B4-K562-CRISPR-A302-983A.png<br>Caption: Western blot following CRISPR against EIF2B4 in K562 whole cell lysate using EIF2B4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2B4. EIF2B4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for EIF2B4 is not avaliable on ENCORE database.



Proteins bound to RNA
  • EIF2B4 RNA is bound by 20 proteins.  







eCLIP assay for EIF2B4 is not avaliable on ENCORE database.





20 proteins are bound to EIF2B4 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr22759182127591874-3'-UTR,CDS,intron-Distal,intron-Proximal6.4769218.17544K562AQR
chr22759193527591999-3'-UTR,CDS,intron-Distal4.399369.30180K562UCHL5
chr22758767327587786-3'-UTR,CDS,intron-Distal,intron-Proximal3.8106123.18197HepG2U2AF2
chr22758725427587366-3'-UTR,CDS,intron-Distal,intron-Proximal3.771965.76765HepG2LARP4
chr22759124327591318-3'-UTR,CDS,intron-Distal,intron-Proximal3.321863.28107HepG2PABPN1
chr22759205527592076-3'-UTR,CDS,intron-Distal4.275883.53690K562ZNF622
chr22758997027590065-3'-UTR,CDS,intron-Distal,intron-Proximal3.362158.83482HepG2GRWD1
chr22758767527587768-3'-UTR,CDS,intron-Distal,intron-Proximal4.366188.32376K562U2AF2
chr22759205027592079-3'-UTR,CDS,intron-Distal,intron-Proximal3.515526.60490HepG2BCLAF1
chr22758767627587759-3'-UTR,CDS,intron-Distal3.378153.50786HepG2G3BP1
chr22758758227587768-3'-UTR,CDS,intron-Distal,intron-Proximal3.209954.54129HepG2PABPN1
chr22759187327591917-3'-UTR,CDS,intron-Distal3.314588.00708HepG2PPIG
chr22759202827592079-3'-UTR,CDS,intron-Distal,intron-Proximal4.150096.62597HepG2GRWD1
chr22759205527592079-3'-UTR,CDS,intron-Distal,intron-Proximal3.071206.41069HepG2PPIG
chr22759155327591608-3'-UTR,CDS,intron-Distal,intron-Proximal5.5578321.27094HepG2PRPF8
chr22759003027590092-3'-UTR,CDS,intron-Distal,intron-Proximal3.311738.46496HepG2BUD13
chr22759191627592028-3'-UTR,CDS,intron-Distal4.1986219.43018HepG2GRWD1
chr22759206227592079-3'-UTR,CDS,intron-Distal,intron-Proximal3.259584.59873K562UCHL5
chr22759156827591621-3'-UTR,CDS,intron-Distal,intron-Proximal5.5010712.60993K562AQR
chr22759155027591617-3'-UTR,CDS,intron-Distal,intron-Proximal3.074845.19487K562PRPF8
chr22758997327590069-3'-UTR,CDS,intron-Distal,intron-Proximal3.758649.30518HepG2PPIG
chr22759123927591308-3'-UTR,CDS,intron-Distal,intron-Proximal3.033485.83127HepG2GRWD1
chr22759183027591875-3'-UTR,CDS,intron-Distal,intron-Proximal5.3782327.60802HepG2PRPF8
chr22759188627592009-3'-UTR,CDS,intron-Distal5.1383215.74878K562ZNF622
chr22758732527587390-3'-UTR,CDS,intron-Distal3.311104.02228HepG2LSM11
chr22758988127590093-3'-UTR,CDS,intron-Distal,intron-Proximal3.2125010.52721HepG2BCLAF1
chr22759157327591620-3'-UTR,CDS,intron-Distal,intron-Proximal4.106494.90219HepG2AQR
chr22759191927592009-3'-UTR,CDS,intron-Distal4.324544.66041HepG2UCHL5
chr22758972427589788-3'-UTR,CDS,intron-Distal3.190343.28107HepG2PABPN1
chr22759192327592030-3'-UTR,CDS,intron-Distal3.529384.77549HepG2PABPN1
chr22759238727592416-5'-UTR,CDS,intron-Distal3.9477914.35874HepG2PPIG
chr22759228227592354-5'-UTR,CDS,intron-Distal4.1765310.19675HepG2TRA2A
chr22759228027592284-5'-UTR,CDS,intron-Distal,intron-Proximal3.086307.77882HepG2PPIG
chr22759228027592412-5'-UTR,CDS,intron-Distal,intron-Proximal4.2381022.24668K562ZNF622
chr22759228027592343-5'-UTR,CDS,intron-Distal,intron-Proximal4.014473.57110HepG2UCHL5
chr22759238427592417-5'-UTR,CDS,intron-Distal,intron-Proximal4.220869.69911HepG2GRWD1
chr22759234327592371-5'-UTR,CDS,intron-Distal4.1131117.19174HepG2PPIG
chr22759063527590689-5'-UTR,CDS,intron-Distal3.095457.26251HepG2GRWD1
chr22759271827592764-5'-UTR,CDS,intron-Distal,intron-Proximal4.828348.50114HepG2SRSF1
chr22759314427593202-5'-UTR,CDS,intron-Distal,intron-Proximal3.296094.22172HepG2SRSF9
chr22759228027592357-5'-UTR,CDS,intron-Distal,intron-Proximal3.7595713.12700K562DDX24
chr22759228127592384-5'-UTR,CDS,intron-Distal3.9496013.31464HepG2GRWD1
chr22759276427592789-5'-UTR,CDS,intron-Distal,intron-Proximal3.681956.33071HepG2SRSF1
chr22759235427592421-5'-UTR,CDS,intron-Distal,intron-Proximal5.6797411.63720HepG2TRA2A
chr22759274127592784-5'-UTR,CDS,intron-Distal,intron-Proximal3.564616.18248K562UCHL5
chr22759228027592379-5'-UTR,CDS,intron-Distal,intron-Proximal4.0775124.08443HepG2BCLAF1
chr22759237927592416-5'-UTR,CDS,intron-Distal3.478176.92192K562UCHL5
chr22759227927592379-5'-UTR,CDS,intron-Distal,intron-Proximal3.9295118.91140K562UCHL5
chr22759227827592343-5'-UTR,CDS,intron-Distal,intron-Proximal3.7601315.31809HepG2BUD13
chr22759236927592416-5'-UTR,CDS,intron-Distal4.048663.22063K562TRA2A
chr22759274427592762-5'-UTR,CDS,intron-Distal3.671884.24220K562ZNF622
chr22759228427592343-5'-UTR,CDS,intron-Distal4.0382032.54697HepG2PPIG
chr22759273827592785-5'-UTR,CDS,intron-Distal,intron-Proximal3.3504812.14610HepG2PPIG
chr22759310327593171-5'-UTR,CDS,intron-Distal4.6094015.79030HepG2PRPF8
chr22759029627590359-5'-UTR,CDS,intron-Distal,intron-Proximal3.625595.60389HepG2PPIG
chr22759274027592786-5'-UTR,CDS,intron-Distal,intron-Proximal3.712563.23099HepG2UCHL5
chr22759229027592342-5'-UTR,CDS,intron-Distal4.125616.81564HepG2PRPF8
chr22759273727592787-5'-UTR,CDS,intron-Distal,intron-Proximal3.050336.55102HepG2GRWD1
chr22759093427591003-CDS,intron-Distal,intron-Proximal4.0065311.60690HepG2GRWD1
chr22759094227591007-CDS,intron-Distal,intron-Proximal3.118853.67697K562YBX3
chr22759149427591510-intron-Distal,intron-Proximal4.580484.02817HepG2PRPF8
chr22758989427589941-intron-Distal,intron-Proximal3.442173.54718K562PRPF8
chr22759164227591758-intron-Distal,intron-Proximal4.2604212.29978HepG2PCBP2
chr22759219327592269-intron-Distal,intron-Proximal4.461287.37756HepG2AQR
chr22759184127591863-intron-Distal,intron-Proximal4.832274.62947HepG2AQR
chr22759044327590481-intron-Distal,intron-Proximal3.884433.02181K562PRPF8
chr22758898627589021-intron-Distal4.538704.07272K562AGGF1
chr22759271527592738-intron-Distal,intron-Proximal4.604434.28193HepG2AQR
chr22759181927591867-intron-Distal,intron-Proximal5.073685.33214K562PRPF8
chr22758781027587900-intron-Distal,intron-Proximal5.3253920.33346HepG2U2AF2
chr22758776827587788-intron-Distal,intron-Proximal3.799223.19080K562U2AF2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for EIF2B4 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for EIF2B4 is not available on ENCORE database.

  • 52 RBPs can affect the levels of EIF2B4.  








The Knockdown/knockout RNA-Seq assay for EIF2B4 is not available on ENCORE database.





52 RBPs can affect the gene expression of EIF2B4 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
EIF2B4ENSG00000115211.11785.611301106.66930-0.494303.651802.62950HepG2BCLAF1
EIF2B4ENSG00000115211.111443.824401244.126100.214702.347501.72690HepG2CKAP4
EIF2B4ENSG00000115211.11883.044001279.76490-0.535302.917502.13180HepG2CNOT7
EIF2B4ENSG00000115211.11785.846601509.67920-0.9419014.3432012.92360HepG2CPSF6
EIF2B4ENSG00000115211.111308.683901076.430300.281802.674901.67590HepG2YBX3
EIF2B4ENSG00000115211.111723.827301957.20360-0.183201.952101.31340HepG2DAZAP1
EIF2B4ENSG00000115211.11908.087301047.63340-0.206201.893001.33500HepG2DNAJC2
EIF2B4ENSG00000115211.11771.171801086.35290-0.494403.193801.99560HepG2AGO1
EIF2B4ENSG00000115211.112330.041401930.998500.270903.655302.71540HepG2EIF4G1
EIF2B4ENSG00000115211.111334.168501849.75560-0.471404.574003.54420HepG2EIF4G2
EIF2B4ENSG00000115211.111607.553102264.30780-0.4942010.061209.03220HepG2ETF1
EIF2B4ENSG00000115211.112094.994201457.948400.522905.728504.75190HepG2HNRNPK
EIF2B4ENSG00000115211.111792.623501462.900800.293101.933101.35970HepG2IGF2BP2
EIF2B4ENSG00000115211.111170.125401665.56090-0.509303.104402.07360HepG2MTPAP
EIF2B4ENSG00000115211.111598.637501940.76510-0.279802.713401.95770HepG2NOL12
EIF2B4ENSG00000115211.111071.584401267.56250-0.242302.498801.90540HepG2PUS1
EIF2B4ENSG00000115211.111371.801101645.11810-0.262102.343201.75970HepG2QKI
EIF2B4ENSG00000115211.112506.419801588.085000.658205.764604.76980HepG2SF3A3
EIF2B4ENSG00000115211.111326.133601616.56560-0.285702.042001.43060HepG2SF3B1
EIF2B4ENSG00000115211.111556.974201776.48820-0.190301.983601.37180HepG2SLBP
EIF2B4ENSG00000115211.111493.798601224.551600.286604.019303.26710HepG2SMN1
EIF2B4ENSG00000115211.111868.466802137.52780-0.194102.289101.81940HepG2SRFBP1
EIF2B4ENSG00000115211.111417.150901650.58030-0.220001.902501.31400HepG2SRSF7
EIF2B4ENSG00000115211.111557.783001342.985000.214002.299001.78610HepG2SRSF9
EIF2B4ENSG00000115211.111308.584401591.75500-0.282601.979001.35320HepG2TARDBP
EIF2B4ENSG00000115211.111461.341501145.756800.350903.607903.05300HepG2TIAL1
EIF2B4ENSG00000115211.112222.057601549.111200.520405.851904.95490HepG2U2AF1
EIF2B4ENSG00000115211.113053.313101743.777200.808102.238401.36120HepG2U2AF2
EIF2B4ENSG00000115211.11964.77700736.386500.389602.555201.82650HepG2UCHL5
EIF2B4ENSG00000115211.111794.486701359.744800.400106.643505.78360HepG2UTP3
EIF2B4ENSG00000115211.111528.608801227.140000.316801.954701.33750HepG2XRN2
EIF2B4ENSG00000115211.111960.145601548.958400.339606.273705.53670HepG2ZNF622
EIF2B4ENSG00000115211.111397.425901048.779900.414005.351704.60420K562AGGF1
EIF2B4ENSG00000115211.112116.951301482.299700.514105.770404.97690K562AQR
EIF2B4ENSG00000115211.113048.126501633.390200.9000032.7974030.80980K562DDX47
EIF2B4ENSG00000115211.111130.76410842.521100.424403.358102.34900K562EFTUD2
EIF2B4ENSG00000115211.111346.17980647.031501.056909.100007.23710K562EIF3A
EIF2B4ENSG00000115211.111160.97370593.224400.9686020.6770018.45120K562EIF3G
EIF2B4ENSG00000115211.111124.34850707.242600.6687011.125809.99870K562FXR1
EIF2B4ENSG00000115211.11360.52600555.05820-0.622503.736702.88750K562G3BP2
EIF2B4ENSG00000115211.11593.90990861.37780-0.536405.063804.27890K562HNRNPC
EIF2B4ENSG00000115211.111012.04100743.345400.445103.293601.93300K562HNRNPK
EIF2B4ENSG00000115211.111799.451901476.050600.285702.395701.41950K562HNRNPL
EIF2B4ENSG00000115211.111667.098201234.135600.433703.254402.23720K562HNRNPM
EIF2B4ENSG00000115211.111162.81540819.869700.504104.367403.42610K562PRPF8
EIF2B4ENSG00000115211.111008.70720760.053400.408203.783903.01890K562PTBP1
EIF2B4ENSG00000115211.111692.609701082.456900.644806.963706.14730K562PUS1
EIF2B4ENSG00000115211.111707.08600835.138701.0313014.5419012.64260K562RPS19
EIF2B4ENSG00000115211.111385.550001040.703200.412803.963402.65880K562SAFB
EIF2B4ENSG00000115211.111582.163801284.451900.300603.127402.26100K562SF3A3
EIF2B4ENSG00000115211.11742.64520577.097700.363802.843201.97650K562SF3B4
EIF2B4ENSG00000115211.111130.332201315.93710-0.219302.310701.44670K562SRPK2
EIF2B4ENSG00000115211.11994.98430745.136800.417103.722402.90720K562SRSF1
EIF2B4ENSG00000115211.111537.302701171.495600.391904.325503.29220K562SYNCRIP
EIF2B4ENSG00000115211.11738.12810537.958500.456302.823001.71780K562U2AF1
EIF2B4ENSG00000115211.111769.783801228.171800.5270011.413109.82760K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for EIF2B4 is not available on ENCORE database.





16 RBPs can affect the splicing of EIF2B4 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
A3SSEIF2B4ENSG00000115211.11chr2-2759089127591069275908912759100627591238275913300.054000.000350.03154longerHepG2SF3A3
A3SSEIF2B4ENSG00000115211.11chr2-2759228027592437275922802759241627592742275927860.155000.000000.00007longerK562U2AF2
A3SSEIF2B4ENSG00000115211.11chr2-2759089127591006275908912759098027591238275913300.078000.000000.00009longerHepG2U2AF2
A3SSEIF2B4ENSG00000115211.11chr2-2759089127591069275908912759100627591238275913300.054000.000530.09830longerHepG2NCBP2
A3SSEIF2B4ENSG00000115211.11chr2-2759089127591069275908912759100627591238275913300.072000.000180.03937longerHepG2TAF15
A3SSEIF2B4ENSG00000115211.11chr2-2759194227592109275919422759207927592280275924160.119000.000000.00000longerK562AQR
A3SSEIF2B4ENSG00000115211.11chr2-2759061227590710275906122759068927590891275909800.081000.000000.00003longerK562AQR
A3SSEIF2B4ENSG00000115211.11chr2-2758994027590118275899402759006827590323275904260.162000.000000.00000longerK562AQR
A3SSEIF2B4ENSG00000115211.11chr2-2759029827590502275902982759042627590612275907100.101000.000000.00000longerK562AQR
A3SSEIF2B4ENSG00000115211.11chr2-2759089127591069275908912759100627591238275912680.143000.000000.00000longerK562AQR
A3SSEIF2B4ENSG00000115211.11chr2-2759123827591412275912382759133027591520275916580.137000.000000.00000longerK562AQR
A3SSEIF2B4ENSG00000115211.11chr2-2759228027592473275922802759241627592742275927860.107000.000000.00000longerK562AQR
A3SSEIF2B4ENSG00000115211.11chr2-2759089127591069275908912759100627591238275913300.057000.000790.04049longerK562PUS1
A3SSEIF2B4ENSG00000115211.11chr2-2759089127591069275908912759100627591238275913300.091000.000000.00017longerHepG2FTO
A3SSEIF2B4ENSG00000115211.11chr2-2758995127590162275899512759006827590323275904260.097000.000010.01032longerHepG2SSB
A3SSEIF2B4ENSG00000115211.11chr2-2759228027592437275922802759241627592742275931930.094000.000540.02747longerK562U2AF1
A3SSEIF2B4ENSG00000115211.11chr2-275899512759016227589951275900682759032327590426-0.079000.000080.03466shorterK562HNRNPK
A3SSEIF2B4ENSG00000115211.11chr2-2759089127591069275908912759100627591238275913300.074000.000180.01864longerHepG2SF3B4
A3SSEIF2B4ENSG00000115211.11chr2-2758995127590162275899512759006827590323275904260.118000.000000.00005longerHepG2SF3B4
A3SSEIF2B4ENSG00000115211.11chr2-275896252758980327589625275897752758994027590068-0.058000.000270.02514shorterHepG2SF3B4
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.110000.000000.00000includedK562PUS1
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.083000.000000.00000includedK562AGGF1
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.054000.002850.03395includedK562PRPF8
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.054000.000000.00001includedK562AKAP8L
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.060000.000000.00012includedHepG2TAF15
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.052000.000000.00000includedHepG2U2AF1
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.117000.000000.00000includedK562RBM15
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.176000.000000.00015includedK562U2AF1
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.233000.000000.00000includedK562U2AF2
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.119000.000000.00000includedK562UTP18
RIEIF2B4ENSG00000115211.11chr2-2759228027592786275922802759241627592742275927860.141000.000000.00000includedK562AQR
MXEEIF2B4ENSG00000115211.11chr2-27590323275904262759123827591388275899402759006827591520275916300.051000.000070.000851includedK562AQR
MXEEIF2B4ENSG00000115211.11chr2-27590612275907102759089127591030275903232759042627591238275913300.087000.000030.000431includedK562AQR
MXEEIF2B4ENSG00000115211.11chr2-27591238275912682759152027591658275908912759100627591942275920790.067000.015810.091321includedK562AQR
MXEEIF2B4ENSG00000115211.11chr2-27591238275913302759152027591600275908912759100627591872275920790.117000.004290.031611includedK562AQR
MXEEIF2B4ENSG00000115211.11chr2-27590891275910062759123827591330275906122759068927591520275916000.142000.002730.035311includedK562U2AF2
MXEEIF2B4ENSG00000115211.11chr2-27590612275906892759089127591006275903232759042627591238275913300.208000.000000.000011includedHepG2U2AF2

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for EIF2B4 is not avaliable on ENCORE database.