ADAT3

General Information

Gene Name       : ADAT3
Gene ID             : ENSG00000213638.5
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: ADAT3)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ADAT3ENSG00000213638.50.000.020.01ENCSR000AEF
HepG2ADAT3ENSG00000213638.50.750.000.38ENCSR985KAT
K562ADAT3ENSG00000213638.50.490.020.26ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq ADAT3-BGKcLV23-K562ENCSR994EWLADAT3K562ReleasedENCSR877JEM   CRISPRKD% = 73.0,73.6
knockdown followed by RNA-seq ADAT3-BGHcLV24-HepG2ENCSR840HSLADAT3HepG2ReleasedENCSR671FDZ   CRISPRKD% = 72.7,80.0






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ADAT3ENCAB860NAKPA5-63626UK2881676BBGHcLV24-7, Released, ENCODE4BGKcLV23-7, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ADAT3K562CRISPRENCSR994EWLENCSR877JEMADAT3-BGKcLV23-7,ADAT3-BGKcLV23-8NT-BGKcLV23-1,NT-BGKcLV23-273.0,73.6ReleasedENCODE4
ADAT3K562CRISPRBGKcLV23-9,BGKcLV23-10BGKcLV23-1,BGKcLV23-20.0,0.0NotSatisfiedENCODE4
ADAT3HepG2CRISPRENCSR840HSLENCSR671FDZADAT3-BGHcLV24-7,ADAT3-BGHcLV24-8NT-BGHcLV24-1,NT-BGHcLV24-272.7,80.0ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ADAT3Product_ID: PA5-63626
Lot_ID: UK2881676B
Source: Thermo Fisher
Target Name: ADAT3-human		 ADAT3-HEPG2-CRISPR-PA5-63626.png<br>Caption: Western blot following CRISPR against ADAT3 in HepG2 whole cell lysate using ADAT3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ADAT3. ADAT3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ADAT3-K562-CRISPR-PA5-63626.png<br>Caption: Western blot following CRISPR against ADAT3 in K562 whole cell lysate using ADAT3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ADAT3. ADAT3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for ADAT3 is not avaliable on ENCORE database.



Proteins bound to RNA
  • ADAT3 RNA is bound by 14 proteins.  







eCLIP assay for ADAT3 is not avaliable on ENCORE database.





14 proteins are bound to ADAT3 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr1919119101912002+3'-UTR,intron-Distal3.740345.59069HepG2SLTM
chr1919133731913433+3'-UTR,5'-UTR,intron-Distal4.155844.33692K562NONO
chr1919054051905474+5'-UTR,intron-Proximal4.514154.82934K562RBM15
chr1919081251908250+5'-UTR,intron-Distal4.684706.64417K562NONO
chr1919053931905442+5'-UTR,intron-Proximal4.7220812.43092HepG2DDX3X
chr1919054101905504+5'-UTR,intron-Proximal4.124067.26133HepG2PRPF8
chr1919080291908197+5'-UTR,intron-Distal3.8709011.90935HepG2HNRNPM
chr1919054321905450+5'-UTR,intron-Proximal4.925216.74224HepG2AQR
chr1919054371905449+5'-UTR,intron-Proximal4.603313.11721K562AQR
chr1919126271912736+CDS,intron-Distal4.143306.66289HepG2SLTM
chr1919098771909966+intron-Distal3.723705.37976K562NONO
chr1919055481905636+intron-Distal5.384425.08709K562GEMIN5
chr1919059631906032+intron-Distal3.189403.84859K562NONO
chr1919101431910188+intron-Distal3.972433.30336K562EWSR1
chr1919099781910068+intron-Distal4.902757.99471K562NONO
chr1919055451905738+intron-Distal4.155833.31384K562XRN2
chr1919092421909298+intron-Distal4.199864.23759K562NONO
chr1919058301905900+intron-Distal3.436784.71513K562NONO
chr1919077951907904+intron-Distal3.267563.99385K562CSTF2T
chr1919102141910294+intron-Distal3.620645.37486K562NONO
chr1919055291905652+intron-Distal,intron-Proximal3.043176.66905HepG2CSTF2T
chr1919078241907895+intron-Distal4.010463.19474K562LSM11
chr1919055811905658+intron-Distal4.074093.53896K562NONO
chr1919055811905699+intron-Distal4.876853.92230K562EFTUD2
chr1919057291905830+intron-Distal4.498094.72743K562NONO
chr1919054671905517+intron-Proximal4.729705.00814HepG2RBM22
chr1919054501905503+intron-Proximal4.931786.03362HepG2AQR
chr1919054491905480+intron-Proximal4.860594.65563K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for ADAT3 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for ADAT3 is not available on ENCORE database.

  • 47 RBPs can affect the levels of ADAT3.  








The Knockdown/knockout RNA-Seq assay for ADAT3 is not available on ENCORE database.





47 RBPs can affect the gene expression of ADAT3 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
ADAT3ENSG00000213638.466.6719025.979401.359602.280901.42170HepG2ABCF1
ADAT3ENSG00000213638.453.7615019.588901.456402.294301.74360HepG2BOP1
ADAT3ENSG00000213638.431.605103.558703.150604.785203.98010HepG2DDX3X
ADAT3ENSG00000213638.420.189400.984004.358603.147202.33030HepG2DDX55
ADAT3ENSG00000213638.418.107803.049602.569802.426301.84420HepG2DDX6
ADAT3ENSG00000213638.47.441900.0000099999.999902.489001.86430HepG2DNAJC2
ADAT3ENSG00000213638.416.297503.251502.325402.018301.46990HepG2EIF4A3
ADAT3ENSG00000213638.42.1397014.82910-2.793002.243701.52900HepG2FUBP3
ADAT3ENSG00000213638.423.944703.269902.872301.904301.32550HepG2FXR1
ADAT3ENSG00000213638.417.847203.064102.542102.281601.64390HepG2HNRNPK
ADAT3ENSG00000213638.45.982900.0000099999.999901.805801.30690HepG2HNRNPU
ADAT3ENSG00000213638.4112.7610022.261902.3405010.475208.85660HepG2LARP4
ADAT3ENSG00000213638.4115.585903.271005.1430025.1513023.30670HepG2MAGOH
ADAT3ENSG00000213638.497.242003.291104.8849019.2032017.65970HepG2MATR3
ADAT3ENSG00000213638.466.648003.370004.3056012.9124011.60170HepG2NCBP2
ADAT3ENSG00000213638.426.244207.143801.877102.372801.80190HepG2PCBP1
ADAT3ENSG00000213638.417.061200.0000099999.999905.748604.72030HepG2PPIL4
ADAT3ENSG00000213638.42.0954012.98680-2.631702.165801.47440HepG2PRPF4
ADAT3ENSG00000213638.424.628303.774102.706004.018103.33180HepG2PUM1
ADAT3ENSG00000213638.417.481300.980504.156102.990101.95710HepG2RBM27
ADAT3ENSG00000213638.431.323108.088201.953202.538202.01700HepG2NELFE
ADAT3ENSG00000213638.414.397000.903403.994102.491901.73020HepG2RPS2
ADAT3ENSG00000213638.411.022600.590304.222802.028901.34390HepG2RPS3A
ADAT3ENSG00000213638.47.512800.0000099999.999902.076101.50520HepG2SAFB2
ADAT3ENSG00000213638.478.7544035.086201.166403.336402.59410HepG2SF3A3
ADAT3ENSG00000213638.416.392200.0000099999.999904.395703.71790HepG2TRIM56
ADAT3ENSG00000213638.476.3792022.410201.768901.964601.42550HepG2U2AF1
ADAT3ENSG00000213638.469.657608.456603.042005.975604.47420HepG2U2AF2
ADAT3ENSG00000213638.422.358203.676502.604302.463001.67690HepG2UBE2L3
ADAT3ENSG00000213638.497.0832030.041601.692201.978001.45400HepG2UTP3
ADAT3ENSG00000213638.492.1573034.221801.429102.687902.14710HepG2ZNF622
ADAT3ENSG00000213638.472.4697014.925602.279508.284707.09400K562AKAP8L
ADAT3ENSG00000213638.449.4563015.660901.658904.134702.99660K562ATP5C1
ADAT3ENSG00000213638.425.650205.572202.202602.992101.99530K562BCLAF1
ADAT3ENSG00000213638.460.403909.008202.745206.945205.10370K562CCAR1
ADAT3ENSG00000213638.438.409504.227303.183502.615801.59030K562CSTF2
ADAT3ENSG00000213638.412.9267041.78380-1.692603.413202.32770K562CELF1
ADAT3ENSG00000213638.4102.8284042.978001.258503.686202.94830K562DDX47
ADAT3ENSG00000213638.443.7284010.139802.108404.494803.60470K562EIF2S2
ADAT3ENSG00000213638.456.4796013.500002.064702.704901.95580K562GRSF1
ADAT3ENSG00000213638.464.6143032.207801.004302.482701.63600K562HNRNPU
ADAT3ENSG00000213638.413.352801.479303.174102.271201.36730K562IGF2BP2
ADAT3ENSG00000213638.416.711501.550903.429603.154702.25570K562MAGOH
ADAT3ENSG00000213638.42.4473018.85410-2.945603.140301.83070K562NCBP2
ADAT3ENSG00000213638.420.025702.934802.770402.260601.57890K562PRPF8
ADAT3ENSG00000213638.425.643501.507004.088706.200905.23970K562PTBP1
ADAT3ENSG00000213638.47.5421039.43360-2.386404.990803.52460K562PUM1
ADAT3ENSG00000213638.447.1557014.800601.671703.271602.68910K562PUS1
ADAT3ENSG00000213638.425.4008067.79500-1.416304.132403.14770K562SF3A3
ADAT3ENSG00000213638.47.2105027.99600-1.957102.741201.39070K562SSRP1
ADAT3ENSG00000213638.413.859901.494703.212902.690901.99200K562TARDBP
ADAT3ENSG00000213638.415.402301.442103.416803.027102.16360K562TIA1
ADAT3ENSG00000213638.417.5253041.79440-1.253902.689001.30850K562UBE2L3
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for ADAT3 is not available on ENCORE database.






No RBPs affect the alternative splcing events of ADAT3 on ENCORE database.





The Knockdown/knockout RNA-Seq assay for ADAT3 is not avaliable on ENCORE database.