DHX57

General Information

Gene Name       : DHX57
Gene ID             : ENSG00000163214.20
Essential Gene : No

Functions

Localization

Domains (AlphaFold: DHX57)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878DHX57ENSG00000163214.2013.2110.7211.96ENCSR000AEF
HepG2DHX57ENSG00000163214.2014.7319.0916.91ENCSR985KAT
K562DHX57ENSG00000163214.2011.2712.0611.66ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq DHX57-BGKcLV17-K562ENCSR359ICPDHX57K562ReleasedENCSR516TLX   CRISPRKD% = 82.1,81.9023796
knockdown followed by RNA-seq DHX57-BGHcLV18-HepG2ENCSR029VGUDHX57HepG2ReleasedENCSR670KAS   CRISPRKD% = 96.3,88.9






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
DHX57ENCAB163YWKPA5-57548UC2739261BReleased, ENCODE4BGHcLV18-11, Released, ENCODE4BGKcLV17-11, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
DHX57K562CRISPRENCSR359ICPENCSR516TLXDHX57-BGKcLV17-11,DHX57-BGKcLV17-12NT-BGKcLV17-1,NT-BGKcLV17-282.1,81.9023796ReleasedENCODE4
DHX57HepG2CRISPRENCSR029VGUENCSR670KASDHX57-BGHcLV18-11,DHX57-BGHcLV18-12NT-BGHcLV18-1,NT-BGHcLV18-296.3,88.9ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DHX57Product_ID: PA5-57548
Lot_ID: UC2739261B
Source: Thermo Fisher
Target Name: DHX57-human		 DHX57-HEPG2-CRISPR-PA5-57548.png<br>Caption: Western blot following CRISPR against DHX57 in HepG2 whole cell lysate using DHX57 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DHX57. DHX57 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 ThermoFisher_PA5-57548_UC2739261B_DHX57.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DHX57 specific antibody, PA5-57548. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DHX57-specific antibody, PA5-57548. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DHX57-K562-CRISPR-PA5-57548.png<br>Caption: Western blot following CRISPR against DHX57 in K562 whole cell lysate using DHX57 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DHX57. DHX57 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for DHX57 is not avaliable on ENCORE database.



Proteins bound to RNA
  • DHX57 RNA is bound by 5 proteins.  







eCLIP assay for DHX57 is not avaliable on ENCORE database.





5 proteins are bound to DHX57 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr23902530339025426-3'-UTR,intron-Distal,intron-Proximal3.260053.68829K562YBX3
chr23908126639081303-5'-UTR,CDS,intron-Distal3.561065.73766K562YBX3
chr23908355739083611-CDS,intron-Distal,intron-Proximal4.185133.45799HepG2U2AF2
chr23908868739088809-CDS,intron-Distal4.512879.30195HepG2BCLAF1
chr23906187039061933-intron-Distal3.728783.16028HepG2SUGP2
chr23908647739086541-intron-Distal3.985533.31570HepG2HNRNPK
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for DHX57 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for DHX57 is not available on ENCORE database.

  • 68 RBPs can affect the levels of DHX57.  








The Knockdown/knockout RNA-Seq assay for DHX57 is not available on ENCORE database.





68 RBPs can affect the gene expression of DHX57 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
DHX57ENSG00000163214.161602.69610799.375301.0035012.5271011.47870HepG2AKAP1
DHX57ENSG00000163214.16932.666501268.21240-0.443406.112705.15550HepG2AUH
DHX57ENSG00000163214.16478.30550796.62470-0.736005.313204.00310HepG2BCCIP
DHX57ENSG00000163214.16693.817401034.98250-0.577005.676904.85730HepG2BOP1
DHX57ENSG00000163214.16784.75300667.241800.233901.878001.31550HepG2CKAP4
DHX57ENSG00000163214.16613.23540950.19000-0.631805.943204.97020HepG2CPSF6
DHX57ENSG00000163214.16473.45040706.02870-0.576504.792403.36220HepG2CPSF7
DHX57ENSG00000163214.16791.784901092.66150-0.464707.117806.00850HepG2DAZAP1
DHX57ENSG00000163214.16848.32330617.225600.458706.159604.98510HepG2DDX19B
DHX57ENSG00000163214.16602.57320765.79740-0.345801.831101.31080HepG2DDX3X
DHX57ENSG00000163214.16700.208301079.00410-0.623803.190002.14230HepG2DDX59
DHX57ENSG00000163214.16444.02630657.70300-0.566803.457102.77420HepG2DDX6
DHX57ENSG00000163214.16483.77360678.87620-0.488803.554502.76400HepG2DNAJC21
DHX57ENSG00000163214.16543.72980672.75940-0.307201.901001.34190HepG2DNAJC2
DHX57ENSG00000163214.16831.23770627.630700.405202.535801.64970HepG2EEF2
DHX57ENSG00000163214.16378.99980632.39770-0.738605.886405.03820HepG2EIF2S1
DHX57ENSG00000163214.16658.702601032.64230-0.648606.797605.54770HepG2EIF4G2
DHX57ENSG00000163214.16982.278101456.14570-0.5680010.317809.27720HepG2ETF1
DHX57ENSG00000163214.161304.53340865.476900.591905.131404.11240HepG2FIP1L1
DHX57ENSG00000163214.16581.00860951.77770-0.712102.709601.70340HepG2FTO
DHX57ENSG00000163214.16970.03040705.005700.460302.326101.68960HepG2FXR1
DHX57ENSG00000163214.16645.84170453.634300.509603.288802.30170HepG2GEMIN5
DHX57ENSG00000163214.16731.573801077.16840-0.558204.341003.55090HepG2GPKOW
DHX57ENSG00000163214.16588.36070762.31650-0.373702.444801.78990HepG2HNRNPC
DHX57ENSG00000163214.16747.087301038.16480-0.474706.446205.41420HepG2HNRNPF
DHX57ENSG00000163214.16697.26210995.32710-0.513508.115507.01650HepG2HNRNPL
DHX57ENSG00000163214.16585.348901027.95680-0.812409.108108.03950HepG2HNRNPLL
DHX57ENSG00000163214.16758.74820597.263800.345202.574801.65580HepG2HNRNPM
DHX57ENSG00000163214.16739.772001022.29360-0.466704.885003.94880HepG2IGF2BP1
DHX57ENSG00000163214.161111.91370709.229600.648604.236903.47240HepG2MATR3
DHX57ENSG00000163214.16529.56120911.44110-0.783408.596007.32960HepG2NSUN2
DHX57ENSG00000163214.161059.47070664.614700.6727011.9747010.85390HepG2PCBP1
DHX57ENSG00000163214.16937.90100676.306400.471705.684504.95670HepG2PTBP1
DHX57ENSG00000163214.16680.133201034.28160-0.604706.662505.79080HepG2PUF60
DHX57ENSG00000163214.16790.074501097.13520-0.473705.087504.19150HepG2PUM2
DHX57ENSG00000163214.16947.41970732.379500.371303.356002.67730HepG2QKI
DHX57ENSG00000163214.16860.79410664.710600.372802.939202.24760HepG2RBM25
DHX57ENSG00000163214.161142.79130752.634100.602409.478808.58560HepG2NELFE
DHX57ENSG00000163214.16958.93780701.030800.451905.473704.64300HepG2RECQL
DHX57ENSG00000163214.16701.270001118.39340-0.673408.124007.05010HepG2RPLP0
DHX57ENSG00000163214.16297.66180597.84460-1.006108.572407.29090HepG2RRP9
DHX57ENSG00000163214.161432.87890825.277400.795905.837804.83370HepG2SF3A3
DHX57ENSG00000163214.16822.816701017.50530-0.306401.900701.30820HepG2SF3B1
DHX57ENSG00000163214.16869.66630704.057500.304702.660402.07850HepG2SF3B4
DHX57ENSG00000163214.16756.60520991.72330-0.390404.834703.94780HepG2SLBP
DHX57ENSG00000163214.161474.04780734.910001.0040028.4049027.24050HepG2SRSF1
DHX57ENSG00000163214.16795.081401094.75060-0.461402.822501.80980HepG2SRSF3
DHX57ENSG00000163214.16557.719001021.20740-0.8727017.6703016.28190HepG2SRSF5
DHX57ENSG00000163214.16667.936001019.97080-0.610709.807008.63460HepG2SRSF7
DHX57ENSG00000163214.16980.201001193.42800-0.284003.130202.59250HepG2SSB
DHX57ENSG00000163214.16521.23870756.04100-0.536502.676101.49480HepG2SUB1
DHX57ENSG00000163214.16494.10880779.72830-0.658104.439403.61320HepG2SUPV3L1
DHX57ENSG00000163214.16602.351801001.79640-0.733908.266007.10100HepG2TARDBP
DHX57ENSG00000163214.16503.05650735.99070-0.549003.514802.96480HepG2TIAL1
DHX57ENSG00000163214.16526.27970742.40660-0.496403.789403.15180HepG2TRIM56
DHX57ENSG00000163214.16335.75160460.64160-0.456302.326101.62880HepG2UCHL5
DHX57ENSG00000163214.161119.90910706.616200.6643011.9238010.81640HepG2UTP3
DHX57ENSG00000163214.16671.78950885.15060-0.397904.158903.48820K562AGGF1
DHX57ENSG00000163214.16736.05790921.02720-0.323402.228801.64000K562AKAP8L
DHX57ENSG00000163214.16492.73500687.78360-0.481104.460402.73350K562BCCIP
DHX57ENSG00000163214.16610.67330845.59570-0.469605.011904.08470K562EIF2S2
DHX57ENSG00000163214.16739.36970949.25280-0.360502.629301.80320K562FIP1L1
DHX57ENSG00000163214.16568.39610801.66050-0.496103.913702.89340K562FMR1
DHX57ENSG00000163214.16869.23770648.533700.422504.270903.54230K562FXR1
DHX57ENSG00000163214.16850.31520635.680500.419602.246201.61840K562G3BP2
DHX57ENSG00000163214.16590.02500752.20750-0.350402.263701.54470K562GPKOW
DHX57ENSG00000163214.16872.305701126.92960-0.369502.802402.04270K562GRSF1
DHX57ENSG00000163214.16981.398901287.04860-0.391203.000002.17310K562HSPD1
DHX57ENSG00000163214.16760.311501077.52470-0.503104.660203.55650K562PPIG
DHX57ENSG00000163214.16619.24660913.49310-0.560904.998704.29590K562PUS1
DHX57ENSG00000163214.16733.14070889.98940-0.279702.114901.58350K562RBM15
DHX57ENSG00000163214.16321.47260607.85380-0.919006.893704.80830K562RRP9
DHX57ENSG00000163214.161081.79180735.094100.557306.430005.36390K562SRSF1
DHX57ENSG00000163214.16533.04270717.65890-0.429003.424302.49890K562TIA1
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for DHX57 is not available on ENCORE database.





5 RBPs can affect the splicing of DHX57 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEDHX57ENSG00000163214.16chr2-3904323639043327390426623904279739046018390461020.140000.000160.00251includedK562AQR
SEDHX57ENSG00000163214.16chr2-3903370039033835390298563903005739040962390410370.148000.003710.09982includedK562TARDBP
SEDHX57ENSG00000163214.16chr2-3904323639043327390426623904279739046018390461020.072000.002380.07260includedK562HNRNPU
SEDHX57ENSG00000163214.16chr2-390460183904610239042662390427973904619039046286-0.246000.000980.03928excludedK562SF3B1
A3SSDHX57ENSG00000163214.16chr2-3904266239042816390426623904279739046018390461240.197000.000320.00251longerK562AQR
A3SSDHX57ENSG00000163214.16chr2-3907541039075566390754103907554439081195390823940.171000.000070.00066longerK562AQR
A3SSDHX57ENSG00000163214.16chr2-3902542139025613390254213902558439029856390300710.091000.003540.02136longerK562AQR
RIDHX57ENSG00000163214.16chr2-3908119539082394390811953908132039082198390823940.115000.001420.03362includedHepG2FTO

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for DHX57 is not avaliable on ENCORE database.