RPS15

General Information

Gene Name       : RPS15
Gene ID             : ENSG00000115268.9
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RPS15)


Antibody Validation

 View validation images for all RPS15 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RPS15ENSG00000115268.9131.81535.02333.41ENCSR000AEF
HepG2RPS15ENSG00000115268.91022.15415.27718.71ENCSR985KAT
K562RPS15ENSG00000115268.91377.52613.47995.50ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RPS15ENCAB674WDFA305-041A1Released, ENCODE4BGHcLV36-65, Submitted, ENCORE2BGKcLV44-57, Submitting, ENCORE2
RPS15ENCAB057VEZA305-042A1Released, ENCODE4BGHcLV36-65, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RPS15K562CRISPRBGKcLV30-65,BGKcLV30-66BGKcLV30-1,BGKcLV30-20.0,0.0NotSatisfiedENCODE4
RPS15K562CRISPRBGKcLV35-53,BGKcLV35-54BGKcLV35-1,BGKcLV35-228.6,15.5NotSatisfiedENCORE2
RPS15K562CRISPRRPS15-BGKcLV44-57,RPS15-BGKcLV44-58NT-BGKcLV44-1,NT-BGKcLV44-262.7,52.7SequencedENCORE2
RPS15K562CRISPRBGKcLV44-59,BGKcLV44-60BGKcLV44-1,BGKcLV44-2NotSatisfiedENCORE2
RPS15HepG2CRISPRBGHcLV32-63,BGHcLV32-64BGHcLV32-1,BGHcLV32-20.0,0.0NotSatisfiedENCODE4
RPS15HepG2CRISPRBGHcLV36-63,BGHcLV36-64BGHcLV36-1,BGHcLV36-20.0,0.0NotSatisfiedENCORE2
RPS15HepG2CRISPRRPS15-BGHcLV36-65,RPS15-BGHcLV36-66NT-BGHcLV36-1,NT-BGHcLV36-252.7,51.3SubmittingENCORE2
RPS15HepG2CRISPRBGHcLV36-67,BGHcLV36-68BGHcLV36-1,BGHcLV36-254.0,53.0NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPS15Product_ID: A305-041A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPS15-human		 RPS15-HEPG2-CRISPR-A305-041A.png<br>Caption: Western blot following CRISPR against RPS15 in HepG2 whole cell lysate using RPS15 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS15. RPS15 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A305-041A_1_RPS15.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPS15 specific antibody, A305-041A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RPS15-specific antibody, A305-041A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RPS15-K562-CRISPR-A305-041A.png<br>Caption: Western blot following CRISPR against RPS15 in K562 whole cell lysate using RPS15 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS15. RPS15 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RPS15Product_ID: A305-042A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPS15-human		 RPS15-HEPG2-CRISPR-A305-042A.png<br>Caption: Western blot following CRISPR against RPS15 in HepG2 whole cell lysate using RPS15 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS15. RPS15 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A305-042A_1_RPS15.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPS15 specific antibody, A305-042A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RPS15-specific antibody, A305-042A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.








RNA Targets

  • eCLIP assay for RPS15 is not avaliable on ENCORE database.



Proteins bound to RNA
  • RPS15 RNA is bound by 17 proteins.  







eCLIP assay for RPS15 is not avaliable on ENCORE database.





17 proteins are bound to RPS15 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr1914403961440404+3'-UTR,CDS3.57976400.00000K562YBX3
chr1914404201440442+3'-UTR,CDS3.93066400.00000K562YBX3
chr1914402771440326+3'-UTR,CDS,intron-Proximal3.862085.15019HepG2EFTUD2
chr1914401781440229+3'-UTR,CDS3.2572410.06439K562PUM1
chr1914401001440178+3'-UTR,CDS3.270865.80774HepG2DDX55
chr1914398141439888+3'-UTR,intron-Distal,intron-Proximal3.989385.29525HepG2RBFOX2
chr1914401781440232+3'-UTR,CDS3.6579011.17747HepG2DDX55
chr1914400171440053+3'-UTR,CDS,intron-Proximal3.7133930.56349K562PABPC4
chr1914400161440052+3'-UTR,CDS,intron-Proximal4.218658.63182HepG2RPS3
chr1914402231440245+3'-UTR,CDS,intron-Proximal3.247788.28327HepG2LARP4
chr1914402231440248+3'-UTR,CDS,intron-Proximal3.2207524.33423K562PABPC4
chr1914403731440396+3'-UTR,CDS3.33946400.00000K562YBX3
chr1914402261440332+3'-UTR,CDS,intron-Proximal4.1991016.25866HepG2PRPF8
chr1914402871440338+3'-UTR,CDS,intron-Proximal3.820608.43886HepG2SF3B4
chr1914403721440442+3'-UTR,CDS3.242119.79461K562METAP2
chr1914402481440252+3'-UTR,CDS,intron-Proximal3.2601925.01270K562PABPC4
chr1914403471440370+3'-UTR,CDS,intron-Proximal3.079614.91193K562GRWD1
chr1914403821440434+3'-UTR,CDS3.1527711.21409K562RPS3
chr1914402051440212+3'-UTR,CDS3.2500055.75530K562YBX3
chr1914403471440366+3'-UTR,CDS,intron-Proximal3.4346025.70899K562PABPC4
chr1914401751440181+3'-UTR,CDS3.2204737.52288K562YBX3
chr1914403471440362+3'-UTR,CDS,intron-Proximal3.0811210.66908K562RPS3
chr1914399501440047+3'-UTR,CDS,intron-Proximal3.078326.12965HepG2AQR
chr1914399941440041+3'-UTR,CDS,intron-Proximal4.141265.64484HepG2PRPF8
chr1914401811440205+3'-UTR,CDS3.1579954.87976K562YBX3
chr1914399491440007+3'-UTR,intron-Proximal4.890046.39664HepG2SF3B4
chr1914404041440420+3'-UTR,CDS3.67444400.00000K562YBX3
chr1914388441438848+5'-UTR,CDS3.67592400.00000K562YBX3
chr1914388831438892+5'-UTR,CDS,intron-Proximal3.0293628.40918K562PABPC4
chr1914388781438942+5'-UTR,CDS,intron-Proximal3.6029410.07874HepG2PRPF8
chr1914388051438809+5'-UTR,CDS,intron-Proximal4.4654052.16889K562PABPC4
chr1914386651438804+5'-UTR,intron-Distal,intron-Proximal3.5815110.36120HepG2SF3B4
chr1914388341438894+5'-UTR,CDS,intron-Proximal3.4618419.38092HepG2RPS3
chr1914384061438428+5'-UTR,CDS,intron-Proximal4.6708253.49850K562PABPC4
chr1914388091438828+5'-UTR,CDS,intron-Proximal3.7571050.23142K562PABPC4
chr1914388481438882+5'-UTR,CDS3.65047400.00000K562YBX3
chr1914388281438883+5'-UTR,CDS3.1121859.21952K562PABPC4
chr1914387711438801+5'-UTR,intron-Proximal4.795275.58015HepG2AQR
chr1914384381438458+5'-UTR,CDS,intron-Proximal4.321663.49003K562PRPF8
chr1914388011438829+5'-UTR,CDS,intron-Proximal3.377575.33249HepG2AQR
chr1914384131438462+5'-UTR,CDS,intron-Proximal3.618724.57180HepG2AQR
chr1914388821438892+5'-UTR,CDS,intron-Proximal3.10221400.00000K562YBX3
chr1914384511438496+5'-UTR,CDS,intron-Proximal3.548066.46344HepG2PRPF8
chr1914384051438428+5'-UTR,CDS,intron-Proximal3.433825.07065HepG2TRA2A
chr1914385951438646+CDS,intron-Distal,intron-Proximal3.121755.54603K562PRPF8
chr1914398351439876+intron-Distal,intron-Proximal5.069204.69961K562SF3B4
chr1914389511439015+intron-Distal,intron-Proximal3.8592011.96791HepG2PRPF8
chr1914398751439933+intron-Distal,intron-Proximal3.786304.14792HepG2XPO5
chr1914389551439014+intron-Distal,intron-Proximal4.018177.40171K562PRPF8
chr1914389631439020+intron-Distal,intron-Proximal4.649156.58773K562RBFOX2
chr1914389601439012+intron-Distal,intron-Proximal3.073864.70509K562AQR
chr1914389271439020+intron-Distal,intron-Proximal4.176529.49794HepG2PRPF4
chr1914389661439036+intron-Distal,intron-Proximal4.195234.72743K562NONO
chr1914389051439034+intron-Distal,intron-Proximal4.5409210.61789HepG2RBFOX2
chr1914389041438937+intron-Proximal5.035845.26064K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RPS15 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RPS15 is not available on ENCORE database.

  • 101 RBPs can affect the levels of RPS15.  








The Knockdown/knockout RNA-Seq assay for RPS15 is not available on ENCORE database.





101 RBPs can affect the gene expression of RPS15 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RPS15ENSG00000115268.53688.244905988.99000-0.699402.167801.69050HepG2AKAP1
RPS15ENSG00000115268.53129.346804954.82380-0.663002.119101.42680HepG2ASCC1
RPS15ENSG00000115268.52121.792301507.872400.492703.264002.62070HepG2BOP1
RPS15ENSG00000115268.51108.503201652.38530-0.575904.522403.56730HepG2CALR
RPS15ENSG00000115268.53023.773704707.60550-0.638602.059501.38520HepG2CIRBP
RPS15ENSG00000115268.51897.368501384.231600.454802.552601.88780HepG2CPSF6
RPS15ENSG00000115268.5883.964501999.70610-1.177703.223401.71760HepG2CSTF2T
RPS15ENSG00000115268.53841.313403376.578300.185902.612601.88880HepG2DAZAP1
RPS15ENSG00000115268.5944.046802149.34440-1.187005.353004.44190HepG2DDX21
RPS15ENSG00000115268.5806.129901223.19600-0.601604.193802.99840HepG2DHX30
RPS15ENSG00000115268.51859.918801256.829400.565407.365906.37190HepG2DNAJC2
RPS15ENSG00000115268.53109.958105233.03860-0.750802.362601.77010HepG2EIF4A3
RPS15ENSG00000115268.54905.305603191.105600.620209.037607.60140HepG2EIF4G2
RPS15ENSG00000115268.54628.241406599.42950-0.5119015.8046014.56090HepG2ETF1
RPS15ENSG00000115268.53317.524404859.56280-0.550704.137403.18430HepG2FUBP3
RPS15ENSG00000115268.53216.483504899.55620-0.607201.864701.31280HepG2G3BP1
RPS15ENSG00000115268.52637.991503103.19200-0.234302.980202.01570HepG2HNRNPA2B1
RPS15ENSG00000115268.52898.773002060.458100.4924010.004308.74790HepG2HNRNPF
RPS15ENSG00000115268.55113.279403075.791900.733208.777407.64160HepG2HNRNPL
RPS15ENSG00000115268.51860.170001414.745800.394803.441102.77260HepG2HNRNPU
RPS15ENSG00000115268.53068.004702028.940500.596507.195906.07700HepG2IGF2BP1
RPS15ENSG00000115268.52782.372904979.51860-0.839702.917302.22780HepG2IGF2BP2
RPS15ENSG00000115268.52378.046604676.17730-0.975602.632501.67530HepG2IGF2BP3
RPS15ENSG00000115268.52147.475601445.130300.571307.851106.68280HepG2ILF2
RPS15ENSG00000115268.54053.193703125.121400.375006.971405.40170HepG2KIF1C
RPS15ENSG00000115268.53287.062905285.91140-0.685402.019501.45520HepG2MAGOH
RPS15ENSG00000115268.54692.632403348.210300.486902.773602.00830HepG2NOL12
RPS15ENSG00000115268.53589.896505066.46660-0.4970013.8323012.64040HepG2PCBP1
RPS15ENSG00000115268.52212.732201261.854800.810206.067905.00940HepG2PPIL4
RPS15ENSG00000115268.52166.008605155.35580-1.2510044.1718042.54150HepG2PTBP1
RPS15ENSG00000115268.54140.981303392.889800.287406.663405.82660HepG2PUM1
RPS15ENSG00000115268.52291.982103482.49970-0.603508.419407.44400HepG2PUS1
RPS15ENSG00000115268.52486.511505583.29970-1.1670049.1432047.14240HepG2QKI
RPS15ENSG00000115268.52709.879305067.23280-0.903006.846005.82580HepG2RBM25
RPS15ENSG00000115268.53616.427205737.38570-0.6658023.8427022.56670HepG2NELFE
RPS15ENSG00000115268.53677.795105343.65010-0.539008.099407.12790HepG2RECQL
RPS15ENSG00000115268.52262.334201259.942800.844405.577404.71630HepG2SAFB2
RPS15ENSG00000115268.55044.837303304.948300.610102.066801.48270HepG2SF3A3
RPS15ENSG00000115268.53606.638705367.32530-0.573507.832006.91820HepG2SF3B4
RPS15ENSG00000115268.54485.260803064.656700.5494019.4340017.89620HepG2SLBP
RPS15ENSG00000115268.54930.155706230.00980-0.337607.843907.11960HepG2SRFBP1
RPS15ENSG00000115268.51568.675305602.35230-1.83650167.09850164.89950HepG2SRSF1
RPS15ENSG00000115268.52533.247802024.409800.323404.468703.61700HepG2SRSF7
RPS15ENSG00000115268.52076.849404557.83510-1.1340066.3045064.34970HepG2SRSF9
RPS15ENSG00000115268.53599.491605409.12530-0.5876012.1984011.27180HepG2SSB
RPS15ENSG00000115268.5584.370202253.69010-1.947304.600603.76120HepG2SUPV3L1
RPS15ENSG00000115268.52043.175205035.68690-1.3014038.1288036.35770HepG2TAF15
RPS15ENSG00000115268.51996.454101459.937800.451402.450201.76260HepG2TARDBP
RPS15ENSG00000115268.52653.799704627.03150-0.8020018.6901016.59730HepG2TIA1
RPS15ENSG00000115268.5988.581601375.00230-0.476002.403101.93140HepG2TIAL1
RPS15ENSG00000115268.52441.930801386.958500.8160014.8329013.67520HepG2TRIM56
RPS15ENSG00000115268.53908.238702829.749500.465705.340504.55380HepG2UTP3
RPS15ENSG00000115268.51736.567202209.18250-0.347302.263501.64780HepG2ZC3H8
RPS15ENSG00000115268.512721.724406089.962501.0627059.1709056.60800K562AGGF1
RPS15ENSG00000115268.56525.643005257.459300.311704.077902.20830K562AKAP1
RPS15ENSG00000115268.510255.398106692.261800.615703.421702.30320K562AKAP8
RPS15ENSG00000115268.513598.470206336.959201.101505.642604.69170K562AKAP8L
RPS15ENSG00000115268.513049.092309056.234400.526902.907302.30730K562AQR
RPS15ENSG00000115268.59933.714906649.141400.579105.687404.32160K562ATP5C1
RPS15ENSG00000115268.55049.108003714.346200.442808.572006.85070K562BCLAF1
RPS15ENSG00000115268.58600.476007277.588500.240902.927301.70890K562CCDC124
RPS15ENSG00000115268.57520.250805835.128900.365907.368405.53540K562CPEB4
RPS15ENSG00000115268.54129.421103256.399900.342605.896003.96720K562DDX27
RPS15ENSG00000115268.56575.058005401.098600.283704.115602.42630K562DDX6
RPS15ENSG00000115268.58668.024106818.538200.346106.789405.31810K562DKC1
RPS15ENSG00000115268.52314.038003125.61470-0.433702.809901.45470K562DNAJC21
RPS15ENSG00000115268.55091.051503415.790200.575602.946901.62250K562EEF2
RPS15ENSG00000115268.54775.755303299.377600.533402.396401.54840K562EFTUD2
RPS15ENSG00000115268.52408.342501659.210400.537403.935702.99430K562EIF3G
RPS15ENSG00000115268.56026.517705012.189400.265803.408602.10300K562EIF4B
RPS15ENSG00000115268.56338.528704858.256200.383604.655802.90350K562EWSR1
RPS15ENSG00000115268.55031.794002974.616100.758308.978707.35720K562FIP1L1
RPS15ENSG00000115268.55147.082605788.03850-0.169301.815901.30510K562FXR1
RPS15ENSG00000115268.5229.777601436.95720-2.6447014.5589012.64640K562G3BP2
RPS15ENSG00000115268.54902.004805766.27740-0.234302.798601.65250K562GLRX3
RPS15ENSG00000115268.510811.567307335.427700.559503.346202.51850K562GRSF1
RPS15ENSG00000115268.57013.516205456.684000.362003.257002.10050K562HNRNPL
RPS15ENSG00000115268.53910.474302447.037600.676203.470602.41710K562HNRNPM
RPS15ENSG00000115268.55431.812404032.424100.429702.450701.69810K562HSPD1
RPS15ENSG00000115268.56326.960102987.788601.082303.908202.60240K562IGF2BP2
RPS15ENSG00000115268.54462.267003038.250400.554402.792901.59490K562IGF2BP3
RPS15ENSG00000115268.510167.907206724.827100.5964011.494809.45760K562KIF1C
RPS15ENSG00000115268.54563.146903138.229500.540006.931205.57390K562MAGOH
RPS15ENSG00000115268.510522.240806437.533600.708809.961607.49900K562MAK16
RPS15ENSG00000115268.54672.284403131.586400.577102.730201.81400K562MATR3
RPS15ENSG00000115268.510480.864307533.542700.476308.633506.84250K562MBNL1
RPS15ENSG00000115268.52854.599002414.735400.241302.076101.31640K562METAP2
RPS15ENSG00000115268.57317.012505280.491600.470503.797802.72070K562NAA15
RPS15ENSG00000115268.54683.501505557.59010-0.246902.614301.41920K562NCBP2
RPS15ENSG00000115268.57701.339406735.791900.193204.859403.90890K562NSUN2
RPS15ENSG00000115268.59323.624104925.160100.920606.347504.66360K562PABPN1
RPS15ENSG00000115268.54152.563803068.468500.436402.641801.32500K562PCBP1
RPS15ENSG00000115268.54096.000105910.74770-0.529102.832901.99070K562PCBP3
RPS15ENSG00000115268.53597.643904533.50980-0.333602.742501.94490K562POLR2G
RPS15ENSG00000115268.57482.266103377.316201.147509.746707.94960K562PPIG
RPS15ENSG00000115268.59394.454505858.690900.681103.432101.74600K562PPP1R8
RPS15ENSG00000115268.55203.351703210.714900.696403.940203.04650K562PRPF8
RPS15ENSG00000115268.54188.140803049.122500.457803.249902.53640K562PTBP1
RPS15ENSG00000115268.513888.710706283.973401.1441027.9144026.17300K562PUS1
RPS15ENSG00000115268.512577.453106123.242201.0384019.7086018.08620K562RBM15
RPS15ENSG00000115268.53784.904002922.750800.372802.611101.79630K562RBM22
RPS15ENSG00000115268.52997.461804176.77460-0.478604.882602.94650K562SDAD1
RPS15ENSG00000115268.52345.294101492.678700.651803.128102.21770K562SF3B4
RPS15ENSG00000115268.5951.446801523.54420-0.679204.384002.26860K562SND1
RPS15ENSG00000115268.55855.363006809.85750-0.217902.274501.41720K562SRPK2
RPS15ENSG00000115268.54576.945202989.360800.614405.099304.14290K562SRSF1
RPS15ENSG00000115268.54138.832602379.943900.7982010.119507.97940K562SRSF4
RPS15ENSG00000115268.54875.465202823.731400.787808.818706.84550K562SRSF5
RPS15ENSG00000115268.5458.238001410.41690-1.622003.921302.05670K562TAF15
RPS15ENSG00000115268.55650.093603024.988200.9012016.6002014.57850K562TARDBP
RPS15ENSG00000115268.5924.409801496.42870-0.694903.538501.72400K562TBRG4
RPS15ENSG00000115268.55126.394702918.058300.8128012.3691010.52440K562TIA1
RPS15ENSG00000115268.54533.844102864.524600.662304.543903.07980K562TRA2A
RPS15ENSG00000115268.5845.207301437.34680-0.766003.658902.73240K562U2AF2
RPS15ENSG00000115268.59948.060706371.851800.642603.654202.10380K562UTP18
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RPS15 is not available on ENCORE database.






No RBPs affect the alternative splcing events of RPS15 on ENCORE database.





The Knockdown/knockout RNA-Seq assay for RPS15 is not avaliable on ENCORE database.