CSTF1

General Information

Gene Name       : CSTF1
Gene ID             : ENSG00000101138.11
Essential Gene : Yes

Functions

Localization(RBPImage: CSTF1)

Domains (AlphaFold: CSTF1)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CSTF1ENSG00000101138.1136.5038.2337.36ENCSR000AEF
HepG2CSTF1ENSG00000101138.1172.4868.6370.56ENCSR985KAT
K562CSTF1ENSG00000101138.1134.7538.0936.42ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CSTF1-BGKcLV21-K562ENCSR529UYQCSTF1K562ReleasedENCSR854OFV   CRISPRKD% = 66.9,69.3677307
knockdown followed by RNA-seq CSTF1-BGHcLV22-HepG2ENCSR751TUXCSTF1HepG2ReleasedENCSR704VPL   CRISPRKD% = 61.1,59.9






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CSTF1ENCAB678HIEA301-250A1BGHcLV22-15, Released, ENCODE4BGKcLV21-13, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CSTF1K562CRISPRENCSR529UYQENCSR854OFVCSTF1-BGKcLV21-13,CSTF1-BGKcLV21-14NT-BGKcLV21-1,NT-BGKcLV21-266.9,69.3677307ReleasedENCODE4
CSTF1HepG2CRISPRENCSR751TUXENCSR704VPLCSTF1-BGHcLV22-15,CSTF1-BGHcLV22-16NT-BGHcLV22-1,NT-BGHcLV22-261.1,59.9ReleasedENCODE4
CSTF1293TshRNABG293LV02-9,BG293LV02-10BG293LV02-1,BG293LV02-2NotSatisfiedENCORE2
CSTF1293TshRNABG293LV03-5,BG293LV03-6BG293LV03-1,BG293LV03-2NotSatisfiedENCORE2
CSTF1293TshRNABG293LV03-7,BG293LV03-8BG293LV03-1,BG293LV03-2NotSatisfiedENCORE2
CSTF1293TshRNABG293LV04-15,BG293LV04-16BG293LV04-1,BG293LV04-2NotSatisfiedENCORE2
CSTF1293TshRNABG293LV04-17,BG293LV04-18BG293LV04-1,BG293LV04-2NotSatisfiedENCORE2
CSTF1293TshRNABG293LV05-103,BG293LV05-104BG293LV05-1,BG293LV05-2NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  
    CSTF1: Click to RBP Image Database
  • Nuclei
  • Nucleoplasm
  • Mutually Exclusive from Nucleolus
  • Cytoplasm
  • Compartment/Organelle
  • Mitochondria
  • P bodies












    • RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
    CSTF1Product_ID: A301-250A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: CSTF1-human    		 CSTF1-HEPG2-CRISPR-A301-250A.png<br>Caption: Western blot following CRISPR against CSTF1 in HepG2 whole cell lysate using CSTF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CSTF1. CSTF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
    		 CSTF1-k562-CRISPR-A301-250A.png<br>Caption: Western blot following CRISPR against CSTF1 in K562 whole cell lysate using CSTF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CSTF1. CSTF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








    RNA Targets

    • eCLIP assay for CSTF1 is not avaliable on ENCORE database.



    Proteins bound to RNA
    • CSTF1 RNA is bound by 9 proteins.  







    eCLIP assay for CSTF1 is not avaliable on ENCORE database.





    9 proteins are bound to CSTF1 RNA:

    Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
    chr205497902754979074+3'-UTR4.087326.12308K562PUM2
    chr205497893154978999+3'-UTR5.452176.79809K562PABPC4
    chr205497893454978984+3'-UTR3.621446.67890K562PUM2
    chr205497917854979225+3'-UTR4.2791511.77823K562PABPC4
    chr205497912454979181+3'-UTR4.5703911.22023K562PUM2
    chr205497934054979493+3'-UTR4.340244.65489K562IGF2BP1
    chr205497894254978991+3'-UTR3.267633.77784K562RBM15
    chr205497880754978846+3'-UTR3.940834.22136HepG2RBM15
    chr205497875154978804+3'-UTR,CDS4.102797.03491K562RBM15
    chr205496772254967781+5'-UTR,intron-Distal,intron-Proximal3.418498.13495HepG2NCBP2
    chr205497056654970616+5'-UTR,CDS,intron-Proximal3.323893.73965HepG2U2AF1
    chr205497051254970636+5'-UTR,CDS,intron-Proximal4.6307410.03577HepG2AQR
    chr205496776654967810+5'-UTR,intron-Distal,intron-Proximal4.282514.28193HepG2AQR
    chr205497059554970699+5'-UTR,CDS,intron-Proximal4.469154.54659HepG2G3BP1
    chr205496778154967848+5'-UTR,intron-Distal,intron-Proximal4.250414.61711HepG2NCBP2
    chr205497411054974167+CDS4.670426.30966K562YBX3
    chr205497403554974110+CDS3.413626.20049K562YBX3
    chr205497859354978653+CDS3.087534.18590HepG2RBM15
    chr205497861254978660+CDS5.332279.68097K562RBM15
    chr205497237254972421+CDS4.184643.11282HepG2G3BP1
    chr205497858754978612+CDS4.404145.16052K562RBM15
    chr205497845954978553+CDS,intron-Proximal4.729054.97857HepG2AQR
    chr205497851754978573+CDS,intron-Proximal3.276485.85084K562RBM15
    ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




    eCLIP assay for CSTF1 is not avaliable on ENCORE database.



    Diffrential Expression

    • The Knockdown/knockout assay for CSTF1 is not available on ENCORE database.

    • 64 RBPs can affect the levels of CSTF1.  








    The Knockdown/knockout RNA-Seq assay for CSTF1 is not available on ENCORE database.





    64 RBPs can affect the gene expression of CSTF1 :

    Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
    CSTF1ENSG00000101138.72202.803801692.869800.379802.528102.01750HepG2AKAP1
    CSTF1ENSG00000101138.71175.389601428.54780-0.281401.943601.43450HepG2BOP1
    CSTF1ENSG00000101138.71166.55250819.430400.509502.645101.89790HepG2CNOT7
    CSTF1ENSG00000101138.71430.554801870.82570-0.387107.126206.01630HepG2DAZAP1
    CSTF1ENSG00000101138.71011.530301393.02010-0.461703.162002.50580HepG2DDX6
    CSTF1ENSG00000101138.7940.207801169.36230-0.314704.086803.32500HepG2DNAJC2
    CSTF1ENSG00000101138.7914.054001339.14910-0.551004.904904.12720HepG2EIF2S1
    CSTF1ENSG00000101138.7937.488201479.91960-0.658606.104605.15850HepG2EIF4A3
    CSTF1ENSG00000101138.7986.819201440.90480-0.546103.570302.79330HepG2EIF4B
    CSTF1ENSG00000101138.71314.432501768.13170-0.427803.845702.89670HepG2EIF4G2
    CSTF1ENSG00000101138.71602.091801946.22430-0.280703.347102.69530HepG2ETF1
    CSTF1ENSG00000101138.7893.666101191.14240-0.414503.578702.85620HepG2HNRNPA1
    CSTF1ENSG00000101138.71418.516301719.40290-0.277503.032902.06120HepG2HNRNPA2B1
    CSTF1ENSG00000101138.7671.183901324.90600-0.9811016.1122014.57140HepG2HNRNPC
    CSTF1ENSG00000101138.71401.384901704.14090-0.282203.813603.03010HepG2HNRNPL
    CSTF1ENSG00000101138.71082.469801418.77890-0.390302.946802.30040HepG2HNRNPLL
    CSTF1ENSG00000101138.7898.535801407.96340-0.648006.618005.61510HepG2IGF2BP2
    CSTF1ENSG00000101138.71545.084502126.94640-0.461103.162402.27190HepG2LARP4
    CSTF1ENSG00000101138.71205.075901493.69070-0.309801.890701.34340HepG2MAGOH
    CSTF1ENSG00000101138.71558.479401855.11360-0.251402.221501.53760HepG2NOL12
    CSTF1ENSG00000101138.71006.454701369.19030-0.444003.393502.59940HepG2PABPC4
    CSTF1ENSG00000101138.7856.273001173.90230-0.455205.731404.70520HepG2PPIL4
    CSTF1ENSG00000101138.71008.549501770.90300-0.8122013.7436012.55270HepG2PUF60
    CSTF1ENSG00000101138.71582.581701879.93240-0.248402.389801.83050HepG2PUM1
    CSTF1ENSG00000101138.71402.241201878.53400-0.421905.079704.18480HepG2PUM2
    CSTF1ENSG00000101138.71333.663901562.23960-0.228201.836801.31210HepG2QKI
    CSTF1ENSG00000101138.71385.598201626.68550-0.231402.107201.63920HepG2RBM22
    CSTF1ENSG00000101138.7924.199701417.85500-0.617408.221807.10910HepG2RBM25
    CSTF1ENSG00000101138.71212.127501495.23220-0.302803.452602.77330HepG2RECQL
    CSTF1ENSG00000101138.71137.488001583.87330-0.477605.208204.30950HepG2RPLP0
    CSTF1ENSG00000101138.7722.32700924.08690-0.355402.608002.01030HepG2RPS19
    CSTF1ENSG00000101138.7861.687801195.38320-0.472203.866102.92520HepG2RPS2
    CSTF1ENSG00000101138.7901.340001172.09960-0.379005.095104.26910HepG2SAFB2
    CSTF1ENSG00000101138.71111.350901404.32100-0.337602.542901.86440HepG2SF3B1
    CSTF1ENSG00000101138.71165.465601501.81310-0.365804.728603.98620HepG2SF3B4
    CSTF1ENSG00000101138.71317.793401698.10340-0.365805.903004.94000HepG2SLBP
    CSTF1ENSG00000101138.71156.021801366.88570-0.241702.875002.22020HepG2SMN1
    CSTF1ENSG00000101138.71618.984601837.28510-0.182501.843001.40880HepG2SRFBP1
    CSTF1ENSG00000101138.71372.423301748.55750-0.349404.754003.97050HepG2SRSF5
    CSTF1ENSG00000101138.71152.627501444.43580-0.325604.238203.40400HepG2SRSF7
    CSTF1ENSG00000101138.7799.811401275.33360-0.6731013.9427012.87330HepG2SRSF9
    CSTF1ENSG00000101138.71751.858201988.54990-0.182801.860501.33180HepG2SUCLG1
    CSTF1ENSG00000101138.7727.134101140.34340-0.649205.250904.36640HepG2SUPV3L1
    CSTF1ENSG00000101138.71048.487801294.67950-0.304302.483901.68900HepG2TIA1
    CSTF1ENSG00000101138.7855.550901278.95730-0.580007.810707.04850HepG2TIAL1
    CSTF1ENSG00000101138.71020.074101290.31130-0.339004.160803.49740HepG2TRIM56
    CSTF1ENSG00000101138.71273.676901730.16360-0.441904.869503.67640HepG2TUFM
    CSTF1ENSG00000101138.7520.51430673.65580-0.372102.052301.39890HepG2UCHL5
    CSTF1ENSG00000101138.71137.745901349.84250-0.246602.632101.94760HepG2UPF1
    CSTF1ENSG00000101138.71550.052101303.221700.250102.874202.26730HepG2UTP3
    CSTF1ENSG00000101138.7995.952401228.56050-0.302803.651502.89690HepG2ZC3H8
    CSTF1ENSG00000101138.71143.161201484.57000-0.377006.413005.67050HepG2ZNF622
    CSTF1ENSG00000101138.71008.81780698.895000.529405.086803.42300K562AARS
    CSTF1ENSG00000101138.71113.45600884.756700.331603.581302.95040K562AGGF1
    CSTF1ENSG00000101138.71159.28560920.632400.332402.561401.93220K562AKAP8L
    CSTF1ENSG00000101138.72629.407101303.334001.0124020.1516018.73720K562AQR
    CSTF1ENSG00000101138.7852.60580690.476100.304202.525101.94120K562FXR1
    CSTF1ENSG00000101138.7364.17920764.81430-1.0705015.1778013.86410K562HNRNPC
    CSTF1ENSG00000101138.71431.122401094.500200.386803.712102.63310K562MBNL1
    CSTF1ENSG00000101138.71059.68930767.166300.465902.813001.92140K562NAA15
    CSTF1ENSG00000101138.71004.06370794.919400.336902.741602.08000K562PTBP1
    CSTF1ENSG00000101138.71436.433401774.83500-0.305202.490201.71790K562PUF60
    CSTF1ENSG00000101138.71298.07860889.592200.545107.731406.77020K562RBM15
    CSTF1ENSG00000101138.71042.14780858.501500.279602.118201.39850K562SYNCRIP
    CSTF1ENSG00000101138.7846.03690623.676300.439802.861401.74850K562U2AF1
    CSTF1ENSG00000101138.7941.22050643.956900.547506.419705.20200K562U2AF2
    Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".






    The Knockdown/knockout RNA-Seq assay for CSTF1 is not available on ENCORE database.





    1 RBPs can affect the splicing of CSTF1 :

    Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
    SECSTF1ENSG00000101138.7chr20+5497207754972167549705765497077754972262549725400.061000.000010.00014includedK562AQR
    A5SSCSTF1ENSG00000101138.7chr20+5497057654970815549705765497077754972262549725400.210000.000000.00000longerK562AQR
    A5SSCSTF1ENSG00000101138.7chr20+549677065496779554967706549677075497057654970815-0.593000.001140.02085shorterK562AQR
    MXECSTF1ENSG00000101138.7chr20+54970538549707775497226254972540549674265496759454972699549728970.051000.000130.001501includedK562AQR
    MXECSTF1ENSG00000101138.7chr20+54970576549707775497226254972540549674265496759454972699549728970.154000.000040.000581includedK562AQR

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".





    The Knockdown/knockout RNA-Seq assay for CSTF1 is not avaliable on ENCORE database.