NCL

General Information

Gene Name : NCL
Gene ID : ENSG00000115053.15
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: NCL)

Not Available

Antibody Validation

View validation images for all NCL antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	NCL	ENSG00000115053.15	868.90	712.03	790.46	ENCSR000AEF
HepG2	NCL	ENSG00000115053.15	1519.12	1387.14	1453.13	ENCSR985KAT
K562	NCL	ENSG00000115053.15	439.75	492.11	465.93	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	NCL-BGKcLV19-K562	ENCSR665CVU	NCL	K562	Released		ENCSR978MMZ	CRISPR	KD% = 66.3,64.2
knockdown followed by RNA-seq	NCL-BGHcLV18-HepG2	ENCSR013PBS	NCL	HepG2	Released		ENCSR670KAS	CRISPR	KD% = 76.6,78.8

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
NCL	ENCAB686POR	A300-710A	1			NotSatisfied, ENCODE4	BGHcLV18-29, Released, ENCODE4	BGKcLV19-25, Released, ENCODE4
NCL		14574S	1			Submitted, ENCORE2	BGHcLV18-29, Submitted, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
NCL	K562	CRISPR			BGKcLV17-33,BGKcLV17-34	BGKcLV17-1,BGKcLV17-2	33.9,34.8	NotSatisfied	ENCODE4
NCL	K562	CRISPR	ENCSR665CVU	ENCSR978MMZ	NCL-BGKcLV19-25,NCL-BGKcLV19-26	NT-BGKcLV19-1,NT-BGKcLV19-2	66.3,64.2	Released	ENCODE4
NCL	K562	CRISPR			BGKcLV19-27,BGKcLV19-28	BGKcLV19-1,BGKcLV19-2	39.9,46.9	NotSatisfied	ENCODE4
NCL	HepG2	CRISPR	ENCSR013PBS	ENCSR670KAS	NCL-BGHcLV18-29,NCL-BGHcLV18-30	NT-BGHcLV18-1,NT-BGHcLV18-2	76.6,78.8	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
NCL	Product_ID: A300-710A Lot_ID: 1 Source: Bethyl Labs Target Name: NCL-human
NCL	Product_ID: 14574S Lot_ID: 1 Source: Cell Signaling Technology Target Name: NCL-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for NCL is not avaliable on ENCORE database.

Proteins bound to RNA

NCL RNA is bound by 66 proteins.

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eCLIP assay for NCL is not avaliable on ENCORE database.

66 proteins are bound to NCL RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr2	232319529	232319566	-	3'-UTR,intron-Distal,intron-Proximal	4.79961	400.00000	K562	PABPC4
chr2	232327912	232328002	-	5'-UTR,CDS,intron-Distal	5.33949	400.00000	K562	UCHL5
chr2	232327912	232328004	-	5'-UTR,CDS,intron-Distal	3.32567	400.00000	HepG2	AQR
chr2	232327915	232328027	-	5'-UTR,CDS,intron-Distal	3.13018	400.00000	K562	ZNF800
chr2	232327913	232327968	-	CDS,intron-Distal	4.66982	400.00000	K562	ZNF622
chr2	232325238	232325261	-	CDS,intron-Distal	3.43859	400.00000	K562	PUM1
chr2	232325272	232325276	-	CDS,intron-Distal,intron-Proximal	3.60168	400.00000	HepG2	AQR
chr2	232325419	232325492	-	CDS,intron-Distal,intron-Proximal	3.47241	400.00000	K562	PUM1
chr2	232325190	232325206	-	CDS,intron-Distal	6.05486	400.00000	K562	UCHL5
chr2	232325379	232325383	-	CDS,intron-Distal,intron-Proximal	3.52226	400.00000	HepG2	AQR
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 742 entries

eCLIP assay for NCL is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for NCL is not available on ENCORE database.
132 RBPs can affect the levels of NCL.

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The Knockdown/knockout RNA-Seq assay for NCL is not available on ENCORE database.

132 RBPs can affect the gene expression of NCL :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
NCL	ENSG00000115053.11	4713.16550	43692.06270	-3.21260	235.59990	232.26900	HepG2	BOP1
NCL	ENSG00000115053.11	8548.34180	19664.83190	-1.20190	51.60420	49.95070	HepG2	TIAL1
NCL	ENSG00000115053.11	19966.42160	31707.16910	-0.66720	50.08510	48.50490	HepG2	ZNF622
NCL	ENSG00000115053.11	17608.27020	31736.68580	-0.84990	46.87470	44.76080	HepG2	SRSF7
NCL	ENSG00000115053.11	16034.02650	29272.71880	-0.86840	40.53010	38.41390	K562	EIF2S2
NCL	ENSG00000115053.11	10934.91500	31138.01950	-1.50970	41.04730	38.05560	K562	BOP1
NCL	ENSG00000115053.11	21139.34230	35935.84830	-0.76550	39.73230	37.65950	HepG2	AUH
NCL	ENSG00000115053.11	9791.70020	18142.77000	-0.88980	39.88560	37.64010	HepG2	DNAJC21
NCL	ENSG00000115053.11	8929.50700	21067.48070	-1.23840	38.36680	35.55790	HepG2	DDX24
NCL	ENSG00000115053.11	17463.57780	27833.95810	-0.67250	36.38770	34.58720	HepG2	UTP3
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 164 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for NCL is not available on ENCORE database.

1 RBPs can affect the splicing of NCL :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
A3SS	NCL	ENSG00000115053.11	chr2	-	232320152	232320376	232320152	232320335	232320720	232320897	0.05100	0.00000	0.00000	longer	K562	AQR
A3SS	NCL	ENSG00000115053.11	chr2	-	232324862	232325032	232324862	232325004	232325188	232325275	0.05900	0.00000	0.00000	longer	K562	AQR
A3SS	NCL	ENSG00000115053.11	chr2	-	232321711	232321866	232321711	232321835	232321996	232322128	0.30900	0.00848	0.04588	longer	K562	AQR
A3SS	NCL	ENSG00000115053.11	chr2	-	232321711	232321928	232321711	232321866	232322353	232322564	-0.22800	0.00988	0.05234	shorter	K562	AQR
A3SS	NCL	ENSG00000115053.11	chr2	-	232322976	232323213	232322976	232323133	232323711	232323904	-0.24800	0.01266	0.06470	shorter	K562	AQR

Showing 1 to 5 of 5 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

NCL

General Information

Functions

Localization

Domains (AlphaFold: NCL)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for NCL is not avaliable on ENCORE database.

66 proteins are bound to NCL RNA:

eCLIP assay for NCL is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for NCL is not available on ENCORE database.

132 RBPs can affect the gene expression of NCL :

The Knockdown/knockout RNA-Seq assay for NCL is not available on ENCORE database.

1 RBPs can affect the splicing of NCL :

The Knockdown/knockout RNA-Seq assay for NCL is not avaliable on ENCORE database.