NSUN4

General Information

Gene Name       : NSUN4
Gene ID             : ENSG00000117481.10
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: NSUN4)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878NSUN4ENSG00000117481.1015.4321.1418.29ENCSR000AEF
HepG2NSUN4ENSG00000117481.1017.8514.2216.04ENCSR985KAT
K562NSUN4ENSG00000117481.1034.6432.1233.38ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
eCLIPeCLIP: 4047ENCSR224QFQNSUN4K562ReleasedENCAB297AWKENCSR290MTQ  
knockdown followed by RNA-seq NSUN4-BGKcLV15-K562ENCSR550VELNSUN4K562ReleasedENCSR404OHQ   CRISPRKD% = 86.1,81.6
knockdown followed by RNA-seq NSUN4-BGHcLV16-HepG2ENCSR504CPZNSUN4HepG2ReleasedENCSR964HKT   CRISPRKD% = 72.9,83.3






  eCLIP  
  RBP    Exp_UID    Cell_Line    ENCODE_ACCESSION    Control_ENCODE_ACCESSION    ENCODE_Antibody_ID    Antibody    Lot_num    Status    Project  
NSUN44047K562ENCSR224QFQENCSR290MTQENCAB297AWK720212SA246721ReleasedENCODE4


  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
NSUN4ENCAB297AWK720212SA246721BGHcLV16-37, Released, ENCODE4BGKcLV15-43, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
NSUN4K562CRISPRENCSR550VELENCSR404OHQNSUN4-BGKcLV15-43,NSUN4-BGKcLV15-44NT-BGKcLV15-1,NT-BGKcLV15-286.1,81.6ReleasedENCODE4
NSUN4HepG2CRISPRENCSR504CPZENCSR964HKTNSUN4-BGHcLV16-37,NSUN4-BGHcLV16-38NT-BGHcLV16-1,NT-BGHcLV16-272.9,83.3ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NSUN4Product_ID: 720212
Lot_ID: SA246721
Source: Thermo Fisher
Target Name: NSUN4-human		 NSUN4-HEPG2-CRISPR-720212.png<br>Caption: Western blot following CRISPR against NSUN4 in HepG2 whole cell lysate using NSUN4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NSUN4. NSUN4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 NSUN4-K562-CRISPRE-720212.png<br>Caption: Western blot following CRISPR against NSUN4 in K562 whole cell lysate using NSUN4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NSUN4. NSUN4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for NSUN4 is not avaliable on ENCORE database.



Proteins bound to RNA
  • NSUN4 RNA is bound by 11 proteins.  







eCLIP assay for NSUN4 is not avaliable on ENCORE database.





11 proteins are bound to NSUN4 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr14682643646826501+3'-UTR,CDS,intron-Proximal4.727946.16445HepG2PPIG
chr14682742246827480+3'-UTR,CDS3.117964.80096K562TIA1
chr14682722046827300+3'-UTR,CDS,intron-Proximal3.254963.13705K562BUD13
chr14682646946826545+3'-UTR,CDS,intron-Proximal4.780034.68690HepG2PRPF8
chr14682786646827894+3'-UTR3.595143.66308K562UPF1
chr14682922946829304+3'-UTR3.862248.52451K562UPF1
chr14683038446830461+3'-UTR3.536414.75109K562UPF1
chr14682649446826560+3'-UTR,CDS,intron-Proximal3.727154.63311K562AQR
chr14682646246826501+3'-UTR,CDS,intron-Proximal3.690923.95301K562ZNF622
chr14682900046829049+3'-UTR3.378664.14964K562UPF1
chr14680653546806580+5'-UTR,CDS3.9464812.79258K562DDX3X
chr14680646746806516+5'-UTR,CDS4.4031910.17596K562DDX3X
chr14680651646806535+5'-UTR,CDS4.6859611.35836K562DDX3X
chr14681047146810607+5'-UTR,CDS,intron-Proximal4.587057.13477K562DDX3X
chr14681076846810839+CDS,intron-Distal,intron-Proximal3.110914.24993HepG2PPIG
chr14681319646813250+intron-Distal4.264513.82619K562ILF3
chr14681549946815589+intron-Distal3.140544.91867K562ILF3
chr14682072846820812+intron-Distal4.672844.85866HepG2QKI
chr14682629646826337+intron-Proximal4.4132310.47661K562SF3B4
chr14682627946826296+intron-Proximal3.884476.26407K562SF3B4
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for NSUN4 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for NSUN4 is not available on ENCORE database.

  • 44 RBPs can affect the levels of NSUN4.  








The Knockdown/knockout RNA-Seq assay for NSUN4 is not available on ENCORE database.





44 RBPs can affect the gene expression of NSUN4 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
NSUN4ENSG00000117481.6566.72980457.771400.307902.144501.45780HepG2AKAP8L
NSUN4ENSG00000117481.6631.98070393.124200.684806.104205.25480HepG2BOP1
NSUN4ENSG00000117481.6540.76770424.187800.350202.040201.37070HepG2CALR
NSUN4ENSG00000117481.6408.43960603.71490-0.563702.690401.93680HepG2CNOT7
NSUN4ENSG00000117481.6177.25250274.88420-0.633003.182602.03340HepG2CSTF2
NSUN4ENSG00000117481.6602.78080758.41480-0.331401.819501.32610HepG2EIF2S1
NSUN4ENSG00000117481.6587.57280838.14940-0.512403.349502.64720HepG2EIF4A3
NSUN4ENSG00000117481.6600.82000845.62150-0.493103.546602.76980HepG2FXR1
NSUN4ENSG00000117481.6543.59330784.45700-0.529203.689502.92490HepG2G3BP1
NSUN4ENSG00000117481.6642.21690832.77680-0.374902.268501.46580HepG2G3BP2
NSUN4ENSG00000117481.6605.72730409.106300.566103.599502.87840HepG2GPKOW
NSUN4ENSG00000117481.6584.05440390.418500.581004.007803.26500HepG2HNRNPLL
NSUN4ENSG00000117481.6582.39030802.02560-0.461702.466001.52920HepG2KHSRP
NSUN4ENSG00000117481.6459.34140845.91660-0.880909.259608.07190HepG2MAGOH
NSUN4ENSG00000117481.6295.32790406.87970-0.462302.528101.36800HepG2NAA15
NSUN4ENSG00000117481.6584.85300794.86610-0.442604.699004.02850HepG2PTBP1
NSUN4ENSG00000117481.6596.07840464.115500.360903.007902.37220HepG2PUS1
NSUN4ENSG00000117481.6627.64700781.29640-0.315902.119801.52390HepG2RBM25
NSUN4ENSG00000117481.6492.67060884.61770-0.8444014.2420013.19500HepG2NELFE
NSUN4ENSG00000117481.6510.53190823.88660-0.690409.949408.89220HepG2RECQL
NSUN4ENSG00000117481.6515.77450386.432000.416402.337201.68630HepG2SF3B1
NSUN4ENSG00000117481.6664.16280827.57560-0.317402.717902.13110HepG2SF3B4
NSUN4ENSG00000117481.6661.18800429.975200.620703.232902.38570HepG2SNRNP70
NSUN4ENSG00000117481.6666.38780863.80270-0.374303.700503.17360HepG2SRSF1
NSUN4ENSG00000117481.6449.39900545.33830-0.279201.964701.36730HepG2SRSF7
NSUN4ENSG00000117481.6573.48270697.30000-0.282002.226201.75450HepG2SSB
NSUN4ENSG00000117481.6547.84520380.560900.525503.563502.75310HepG2TARDBP
NSUN4ENSG00000117481.6548.39960420.809800.382002.993802.41220HepG2TRIM56
NSUN4ENSG00000117481.6725.67350396.167600.873102.367001.46330HepG2U2AF2
NSUN4ENSG00000117481.6678.26000570.312400.250001.960101.47800HepG2ZNF622
NSUN4ENSG00000117481.63764.059103172.977700.246402.633101.68210K562CELF1
NSUN4ENSG00000117481.62434.564801965.743000.308504.466503.66570K562DDX47
NSUN4ENSG00000117481.6903.503201215.36990-0.427803.346302.33850K562EFTUD2
NSUN4ENSG00000117481.61379.900301642.16130-0.251002.518201.82620K562EIF2S2
NSUN4ENSG00000117481.61249.46300983.328900.345503.664502.75810K562EIF3G
NSUN4ENSG00000117481.61608.764002188.26590-0.443805.250104.20400K562GRSF1
NSUN4ENSG00000117481.61422.718501698.88230-0.255902.498801.64900K562HNRNPU
NSUN4ENSG00000117481.61138.378601433.18210-0.332204.258903.27410K562MTPAP
NSUN4ENSG00000117481.61306.952601750.57600-0.421603.354902.43930K562PCBP3
NSUN4ENSG00000117481.61020.482201342.71470-0.395903.963202.99880K562POLR2G
NSUN4ENSG00000117481.6826.139501182.69050-0.517604.375903.43400K562PRPF8
NSUN4ENSG00000117481.61004.183001377.85000-0.456403.990102.64280K562NELFE
NSUN4ENSG00000117481.61927.056401571.261100.294403.316702.42750K562SF3A3
NSUN4ENSG00000117481.61355.893301609.78410-0.247603.077802.09530K562SRPK2
NSUN4ENSG00000117481.61650.243601170.632500.495304.078402.69430K562U2AF1
NSUN4ENSG00000117481.61861.210901208.873500.6225010.461608.90030K562U2AF2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for NSUN4 is not available on ENCORE database.





5 RBPs can affect the splicing of NSUN4 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SENSUN4ENSG00000117481.6chr1+4680699446807169468064784680659146810472468108160.098000.000560.07308includedK562DROSHA
SENSUN4ENSG00000117481.6chr1+4680699446807169468064784680659146810472468108160.156000.000130.01584includedK562SUPV3L1
SENSUN4ENSG00000117481.6chr1+4680699446807169468064784680659146810472468108160.170000.000790.01592includedK562HNRNPC
A5SSNSUN4ENSG00000117481.6chr1+468104724681081646810472468106344681259246812747-0.079000.000280.00641shorterK562AQR
MXENSUN4ENSG00000117481.6chr1+4681259246812747468185394681870046810472468108164682637546826500-0.260000.000010.001192includedK562PHF6

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for NSUN4 is not avaliable on ENCORE database.