PCBP2

General Information

Gene Name : PCBP2
Gene ID : ENSG00000197111.15
Essential Gene : Yes

Functions

Splicing regulation
Translation regulation
Viral RNA regulation

Localization(RBPImage: PCBP2)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Speckles
Cytoplasm
Cytosolic
Nuclear release mitosis

Domains (AlphaFold: PCBP2)

KH

Antibody Validation

View validation images for all PCBP2 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	PCBP2	ENSG00000197111.15	397.07	468.62	432.85	ENCSR000AEF
HepG2	PCBP2	ENSG00000197111.15	493.40	427.05	460.23	ENCSR985KAT
K562	PCBP2	ENSG00000197111.15	579.75	558.93	569.34	ENCSR000AEO

Assays

4 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 345	ENCSR339FUY	PCBP2	HepG2	Released	ENCAB000BFY	ENCSR115GAA
knockdown followed by RNA-seq	PCBP2-BGKLV11-K562	ENCSR648QFY	PCBP2	K562	Released		ENCSR129RWD	shRNA	KD% = 46.5,41.7
knockdown followed by RNA-seq	PCBP2-BGHLV14-HepG2	ENCSR028ITN	PCBP2	HepG2	Released		ENCSR491FOC	shRNA	KD% = 75.5,63.5
RNA Bind-n-Seq	RBNS: PCBP2	ENCSR673FLQ	PCBP2	cell-free sample	Released		ENCSR265XUT

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
PCBP2	345	HepG2	ENCSR339FUY	ENCSR115GAA	ENCAB000BFY	RN025P	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
PCBP2	ENCAB000BFY	RN025P	1		Released, ENCODE3	Released, ENCODE3	BGHLV14-29, Released, ENCODE3	BGKLV11-31, Released, ENCODE3
PCBP2	ENCAB000AVQ	ARP40568_T100	QC10208		Released, ENCODE3	Released, ENCODE3	BGHLV14-29, Released, ENCODE3	BGKLV11-31, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
PCBP2	K562	shRNA	ENCSR648QFY	ENCSR129RWD	PCBP2-LV11-31,PCBP2-LV11-32	Nontarget-LV11-1,nontarget-LV11-2	46.5,41.7	Released	ENCODE3
PCBP2	HepG2	shRNA			BGHLV12-57,BGHLV12-58	BGHLV12-1,BGHLV12-2		NotSatisfied	ENCODE3
PCBP2	HepG2	shRNA	ENCSR028ITN	ENCSR491FOC	PCBP2_BGHLV14-29,PCBP2_BGHLV14-30	NT_BGHLV14-1,NT_BGHLV14-2	75.5,63.5	Released	ENCODE3

RNA Bind-n-Seqn

GeneName	gene_aliases	Status	Project	Note
PCBP2	PCBP2		Released	ENCODE3

Localization Image in HepG2

PCBP2: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Speckles

Cytoplasm

Cytosolic

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
PCBP2	Product_ID: RN025P Lot_ID: 1 Source: MBLI Target Name: PCBP2-human
PCBP2	Product_ID: ARP40568_T100 Lot_ID: QC10208 Source: Aviva Target Name: PCBP2-human

RNA Targets

PCBP2 protein binds to 8,654 RNA regions.

CanvasJS.com

The binding motifs of PCBP2

Proteins bound to RNA

PCBP2 RNA is bound by 53 proteins.

CanvasJS.com

PCBP2 protein has 8,654 RNA targets:

Only display 100 entries as example. Please click "View All" button to see all 8,654 entries.

Show entries

Search:

RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
PCBP2	chr1	32799874	32799929	-	4.90305	400.00000	HepG2	MARCKSL1	3'-UTR,intron-Distal
PCBP2	chr11	2153688	2153695	-	4.17154	400.00000	HepG2	INS-IGF2, IGF2	3'-UTR,intron-Distal,intron-Proximal
PCBP2	chr17	79222938	79222999	-	7.28348	99.77527	HepG2	SLC38A10	intron-Distal
PCBP2	chr16	87734136	87734225	-	8.28186	66.03546	HepG2	KLHDC4, FLJ00104	3'-UTR,intron-Distal
PCBP2	chr16	69737755	69737813	+	5.08022	58.12994	HepG2	NFAT5	3'-UTR
PCBP2	chr20	62573157	62573175	-	5.08014	53.52575	HepG2	UCKL1	intron-Distal
PCBP2	chr16	18818032	18818108	-	4.61262	50.72455	HepG2	SMG1	3'-UTR,intron-Distal
PCBP2	chr12	49434206	49434257	-	5.23136	50.06858	HepG2	KMT2D	CDS,intron-Distal
PCBP2	chr11	64895324	64895395	-	4.72579	40.96928	HepG2	SYVN1	3'-UTR,intron-Distal
PCBP2	chr8	62577154	62577197	-	6.08937	40.06010	HepG2	ASPH	intron-Distal,intron-Proximal

Showing 1 to 10 of 100 entries

53 proteins are bound to PCBP2 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr12	53848511	53848572	+	3'-UTR,5'-UTR	4.73052	400.00000	HepG2	DDX59
chr12	53848526	53848556	+	3'-UTR,5'-UTR	3.83056	400.00000	K562	ILF3
chr12	53848517	53848527	+	3'-UTR,5'-UTR	4.71288	400.00000	K562	HNRNPK
chr12	53848549	53848566	+	3'-UTR,5'-UTR	3.55349	400.00000	K562	CSTF2T
chr12	53848517	53848526	+	3'-UTR,5'-UTR	3.84625	400.00000	K562	CSTF2T
chr12	53848517	53848526	+	3'-UTR,5'-UTR	4.35286	400.00000	K562	ILF3
chr12	53848508	53848511	+	3'-UTR,5'-UTR,intron-Proximal	5.20488	400.00000	HepG2	DDX59
chr12	53848526	53848549	+	3'-UTR,5'-UTR	3.52067	400.00000	K562	CSTF2T
chr12	53848509	53848560	+	3'-UTR,5'-UTR,intron-Proximal	4.44481	400.00000	K562	PCBP1
chr12	53848509	53848511	+	3'-UTR,5'-UTR,intron-Proximal	3.91860	400.00000	K562	CSTF2T
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 469 entries

The PCBP2 protein binds to its own RNA.

Show entries

Search:

RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
PCBP2	chr12	53846032	53846037	+	6.85959	400.00000	HepG2	PCBP2, RP11-793H13.8	5'-UTR,intron-Distal
PCBP2	chr12	53846125	53846129	+	6.51795	400.00000	HepG2	PCBP2, RP11-793H13.8	5'-UTR,intron-Distal
PCBP2	chr12	53846129	53846165	+	6.67619	400.00000	HepG2	RP11-793H13.8, PCBP2	5'-UTR,intron-Distal,intron-Proximal
PCBP2	chr12	53845990	53846032	+	7.02946	400.00000	HepG2	PCBP2, RP11-793H13.8	5'-UTR,intron-Distal
PCBP2	chr12	53846113	53846118	+	6.26964	400.00000	HepG2	RP11-793H13.8, PCBP2	5'-UTR,intron-Distal
PCBP2	chr12	53846118	53846125	+	6.27618	400.00000	HepG2	RP11-793H13.8, PCBP2	5'-UTR,intron-Distal
PCBP2	chr12	53846037	53846082	+	6.59278	400.00000	HepG2	RP11-793H13.8, PCBP2	5'-UTR,intron-Distal
PCBP2	chr12	53846093	53846113	+	5.78854	83.69471	HepG2	PCBP2, RP11-793H13.8	5'-UTR,intron-Distal
PCBP2	chr12	53858988	53859067	+	8.01215	68.88896	HepG2	RP11-793H13.8, PCBP2	intron-Distal
PCBP2	chr12	53846082	53846093	+	5.72045	57.70086	HepG2	PCBP2, RP11-793H13.8	5'-UTR,intron-Distal

Showing 1 to 10 of 19 entries

Diffrential Expression

6,038 differentially expressed genes after Knockdown/Knockout PCBP2 protein.

CanvasJS.com

107 RBPs can affect the levels of PCBP2.

CanvasJS.com

6,038 differentially expressed genes after Knockdown/Knockout PCBP2 protein :

Only display 100 entries as example. Please click "View All" button to see all 6,038 entries.

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
ANLN	ENSG00000011426.6	741.64980	2387.58870	-1.68670	26.51850	23.75800	HepG2	PCBP2
BRCA1	ENSG00000012048.15	343.94990	1026.99540	-1.57820	20.02770	17.62750	HepG2	PCBP2
DEPDC1	ENSG00000024526.12	154.42200	496.77510	-1.68570	17.42030	15.17410	HepG2	PCBP2
POLA2	ENSG00000014138.4	108.21920	375.97360	-1.79670	17.00290	14.76950	HepG2	PCBP2
HMGB3	ENSG00000029993.10	368.33640	975.25320	-1.40480	16.14750	13.95890	HepG2	PCBP2
GAS7	ENSG00000007237.14	304.31510	95.92040	1.66560	13.64760	11.61080	HepG2	PCBP2
TMEM98	ENSG00000006042.7	617.26900	1425.82090	-1.20780	13.43820	11.42860	HepG2	PCBP2
GRAMD1B	ENSG00000023171.10	59.42170	3.45050	4.10600	12.89490	10.90540	HepG2	PCBP2
FAM214A	ENSG00000047346.8	930.51490	396.13670	1.23190	12.64100	10.66390	HepG2	PCBP2
KITLG	ENSG00000049130.9	473.45170	182.19720	1.37760	12.27290	10.31290	HepG2	PCBP2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

107 RBPs can affect the gene expression of PCBP2 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
PCBP2	ENSG00000197111.11	6509.48650	15960.27670	-1.29390	103.10590	100.34410	HepG2	PCBP1
PCBP2	ENSG00000197111.11	4824.03940	14315.99190	-1.56930	89.77330	86.58080	HepG2	PUM2
PCBP2	ENSG00000197111.11	9713.80280	16907.77590	-0.79960	42.73170	40.93610	HepG2	SF3B4
PCBP2	ENSG00000197111.11	10911.45600	17648.39420	-0.69370	37.97820	36.67260	HepG2	SRSF1
PCBP2	ENSG00000197111.11	4198.05870	8448.19930	-1.00890	36.99930	35.55540	HepG2	TIAL1
PCBP2	ENSG00000197111.11	7100.88830	17929.75340	-1.33630	34.33590	32.40460	HepG2	DDX3X
PCBP2	ENSG00000197111.11	5763.31090	12344.04120	-1.09880	33.60350	30.26600	K562	PCBP2
PCBP2	ENSG00000197111.11	5356.80990	9040.12060	-0.75500	31.38650	29.59650	HepG2	ZC3H8
PCBP2	ENSG00000197111.11	10134.90900	16240.63830	-0.68030	29.28790	27.88040	HepG2	PTBP1
PCBP2	ENSG00000197111.11	11311.61060	18073.88890	-0.67610	28.50930	27.14810	HepG2	NELFE
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 130 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

722 alternative splicing events after Knockdown/Knockout PCBP2 protein :

Only display 100 entries as example. Please click "View All" button to see all 722 entries.

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	ACSL4	ENSG00000068366.15	chrX	-	108934231	108934360	108926364	108926601	108939372	108939425	0.08300	0.00011	included	HepG2	PCBP2
SE	PLD1	ENSG00000075651.11	chr3	-	171404474	171404588	171395355	171395484	171405160	171405374	0.12400	0.00001	included	HepG2	PCBP2
SE	CIZ1	ENSG00000148337.15	chr9	-	130950141	130950213	130947825	130948055	130952607	130952723	0.38300	0.00000	included	HepG2	PCBP2
SE	SREK1	ENSG00000153914.11	chr5	+	65451892	65454760	65449290	65449424	65455046	65455162	0.30800	0.00003	included	HepG2	PCBP2
SE	SEC31A	ENSG00000138674.12	chr4	-	83782783	83782861	83778841	83778917	83784470	83784545	0.19300	0.00003	included	HepG2	PCBP2
SE	DENND4A	ENSG00000174485.10	chr15	-	65992881	65993010	65989604	65989721	65993388	65993530	0.33400	0.00143	included	HepG2	PCBP2
SE	TANK	ENSG00000136560.9	chr2	+	162017795	162017923	162016859	162016997	162036124	162036272	0.18300	0.00000	included	HepG2	PCBP2
SE	ZNF687	ENSG00000143373.13	chr1	+	151257681	151258550	151254787	151254871	151258750	151260882	0.46100	0.00089	included	HepG2	PCBP2
SE	ABCC6	ENSG00000091262.10	chr16	-	16314129	16314245	16313678	16313804	16315505	16315688	0.17900	0.00147	included	HepG2	PCBP2
SE	HNRNPD	ENSG00000138668.14	chr4	-	83292680	83292737	83280721	83280792	83294598	83295109	0.11900	0.00020	included	HepG2	PCBP2

Showing 1 to 10 of 100 entries

54 RBPs can affect the splicing of PCBP2 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	PCBP2	ENSG00000197111.11	chr12	+	53861588	53861627	53861004	53861077	53862560	53862616	0.12900	0.00000	included	K562	SRSF1
SE	PCBP2	ENSG00000197111.11	chr12	+	53859715	53859757	53856276	53856351	53861004	53861312	-0.05300	0.00008	excluded	K562	SF3B4
SE	PCBP2	ENSG00000197111.11	chr12	+	53859715	53859757	53856264	53856351	53861004	53861077	-0.06900	0.00008	excluded	K562	PRPF8
SE	PCBP2	ENSG00000197111.11	chr12	+	53861588	53861627	53861004	53861077	53862560	53862616	-0.10800	0.00003	excluded	K562	WDR3
SE	PCBP2	ENSG00000197111.11	chr12	+	53861588	53861627	53861004	53861077	53862560	53862616	-0.28000	0.00000	excluded	HepG2	U2AF1
SE	PCBP2	ENSG00000197111.11	chr12	+	53858543	53858636	53856276	53856351	53859715	53859757	-0.14100	0.00000	excluded	HepG2	U2AF1
SE	PCBP2	ENSG00000197111.11	chr12	+	53861588	53861627	53861004	53861077	53862560	53862616	-0.12600	0.00000	excluded	K562	AKAP8L
SE	PCBP2	ENSG00000197111.11	chr12	+	53861588	53861627	53861004	53861077	53862560	53862616	-0.28400	0.00000	excluded	HepG2	DDX59
SE	PCBP2	ENSG00000197111.11	chr12	+	53861588	53861627	53861004	53861077	53862560	53862616	-0.22300	0.00006	excluded	HepG2	U2AF2
SE	PCBP2	ENSG00000197111.11	chr12	+	53858543	53858636	53856276	53856351	53859715	53859757	-0.18200	0.00000	excluded	HepG2	U2AF2

Showing 1 to 10 of 91 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The PCBP2 protein affects to its own RNA splicing after knockdown/knockout.

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
SE	PCBP2	ENSG00000197111.11	chr12	+	53861588	53861627			53861004	53861077	53862560	53862616	0.11000	0.00002	0.00436	included	HepG2	PCBP2

Showing 1 to 1 of 1 entries