DDX18

General Information

Gene Name : DDX18
Gene ID : ENSG00000088205.12
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: DDX18)

Not Available

Antibody Validation

Bethyl Labs: A300-636A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	DDX18	ENSG00000088205.12	74.90	69.12	72.01	ENCSR000AEF
HepG2	DDX18	ENSG00000088205.12	66.84	113.18	90.01	ENCSR985KAT
K562	DDX18	ENSG00000088205.12	90.48	104.62	97.55	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	DDX18-BGKcLV28-K562	ENCSR300LXO	DDX18	K562	Released		ENCSR220DTX	CRISPR	KD% = 83.6,83.8
knockdown followed by RNA-seq	DDX18-BGHcLV29-HepG2	ENCSR992YRL	DDX18	HepG2	Released		ENCSR462MFN	CRISPR	KD% = 77.0,84.5

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
DDX18	ENCAB781GEV	A300-636A	1			Released, ENCODE4	BGHcLV29-71, Released, ENCODE4	BGKcLV28-71, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
DDX18	K562	CRISPR	ENCSR300LXO	ENCSR220DTX	DDX18-BGKcLV28-71,DDX18-BGKcLV28-72	NT-BGKcLV28-1,NT-BGKcLV28-2	83.6,83.8	Released	ENCODE4
DDX18	HepG2	CRISPR	ENCSR992YRL	ENCSR462MFN	DDX18-BGHcLV29-71,DDX18-BGHcLV29-72	NT-BGHcLV29-1,NT-BGHcLV29-2	77.0,84.5	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
DDX18	Product_ID: A300-636A Lot_ID: 1 Source: Bethyl Labs Target Name: DDX18-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for DDX18 is not avaliable on ENCORE database.

Proteins bound to RNA

DDX18 RNA is bound by 28 proteins.

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eCLIP assay for DDX18 is not avaliable on ENCORE database.

28 proteins are bound to DDX18 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr2	118572329	118572439	+	5'-UTR,CDS,intron-Proximal	3.49137	25.42358	K562	ZNF622
chr2	118577298	118577371	+	CDS,intron-Proximal	4.39497	16.95425	HepG2	BCLAF1
chr2	118582197	118582286	+	CDS,intron-Proximal	4.13808	16.85893	HepG2	BCLAF1
chr2	118575029	118575103	+	CDS	4.77650	16.44959	HepG2	BCLAF1
chr2	118577292	118577372	+	CDS,intron-Proximal	5.13984	16.43851	K562	ZNF622
chr2	118572332	118572383	+	5'-UTR,CDS	3.92691	16.39160	K562	YBX3
chr2	118579629	118579676	+	CDS,intron-Proximal	4.31164	16.17387	HepG2	PRPF8
chr2	118578735	118578819	+	CDS,intron-Proximal	4.29145	15.85384	HepG2	BCLAF1
chr2	118572338	118572437	+	5'-UTR,CDS	3.50308	14.75988	HepG2	BCLAF1
chr2	118579477	118579574	+	CDS	3.95778	14.30125	HepG2	BCLAF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 108 entries

eCLIP assay for DDX18 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for DDX18 is not available on ENCORE database.
100 RBPs can affect the levels of DDX18.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for DDX18 is not available on ENCORE database.

100 RBPs can affect the gene expression of DDX18 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
DDX18	ENSG00000088205.8	5239.05060	2818.63720	0.89420	44.44060	43.04660	HepG2	SRSF1
DDX18	ENSG00000088205.8	4307.55520	2609.59110	0.72290	22.40020	20.79600	K562	FXR1
DDX18	ENSG00000088205.8	1564.27710	2465.75200	-0.65650	22.46470	20.76860	HepG2	DNAJC2
DDX18	ENSG00000088205.8	2754.06530	4140.47640	-0.58820	22.09620	19.85300	K562	SRPK2
DDX18	ENSG00000088205.8	1958.83470	2834.81090	-0.53330	17.77130	16.09730	HepG2	ILF2
DDX18	ENSG00000088205.8	2980.79840	4342.82070	-0.54290	15.77770	14.22790	K562	UPF1
DDX18	ENSG00000088205.8	1657.77280	2696.75240	-0.70200	15.07580	14.07350	HepG2	TIAL1
DDX18	ENSG00000088205.8	3584.23670	5384.20760	-0.58710	15.38870	13.89900	K562	EIF2S2
DDX18	ENSG00000088205.8	2016.32930	3007.08860	-0.57660	14.48850	13.02150	HepG2	HNRNPF
DDX18	ENSG00000088205.8	7014.39810	4967.46880	0.49770	14.27970	12.91370	K562	DDX47
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 120 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for DDX18 is not available on ENCORE database.

11 RBPs can affect the splicing of DDX18 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	DDX18	ENSG00000088205.8	chr2	+	118580328	118580421	118579722	118579837	118582144	118582284	0.63400	0.00000	0.00000	included	HepG2	U2AF1
SE	DDX18	ENSG00000088205.8	chr2	+	118580328	118580421	118579722	118579837	118582144	118582284	0.08300	0.00000	0.00000	included	HepG2	U2AF2
SE	DDX18	ENSG00000088205.8	chr2	+	118580328	118580421	118579722	118579837	118582144	118582284	0.15500	0.00000	0.00000	included	HepG2	SFPQ
SE	DDX18	ENSG00000088205.8	chr2	+	118580328	118580421	118579722	118579837	118582144	118582284	0.05500	0.00000	0.00000	included	K562	U2AF1
SE	DDX18	ENSG00000088205.8	chr2	+	118580328	118580421	118579722	118579837	118582144	118582284	0.10100	0.00000	0.00065	included	K562	SAFB
A3SS	DDX18	ENSG00000088205.8	chr2	+	118579413	118579637	118579437	118579637	118579156	118579326	0.15200	0.00000	0.00000	longer	K562	AQR
A5SS	DDX18	ENSG00000088205.8	chr2	+	118583064	118583271	118583064	118583175	118583844	118587042	0.13100	0.00000	0.00000	longer	K562	AQR
RI	DDX18	ENSG00000088205.8	chr2	+	118579722	118580356	118579722	118579837	118580328	118580356	-0.43800	0.00001	0.00013	excluded	HepG2	U2AF1
SE	DDX18	ENSG00000088205.8	chr2	+	118580328	118580421	118579722	118579837	118582144	118582284	0.07300	0.00002	0.00413	included	HepG2	TBRG4
SE	DDX18	ENSG00000088205.8	chr2	+	118580328	118580421	118579722	118579837	118582144	118582284	0.06000	0.00002	0.00535	included	HepG2	PCBP2

Showing 1 to 10 of 18 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

DDX18

General Information

Functions

Localization

Domains (AlphaFold: DDX18)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for DDX18 is not avaliable on ENCORE database.

28 proteins are bound to DDX18 RNA:

eCLIP assay for DDX18 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for DDX18 is not available on ENCORE database.

100 RBPs can affect the gene expression of DDX18 :

The Knockdown/knockout RNA-Seq assay for DDX18 is not available on ENCORE database.

11 RBPs can affect the splicing of DDX18 :

The Knockdown/knockout RNA-Seq assay for DDX18 is not avaliable on ENCORE database.