SRSF1

General Information

Gene Name : SRSF1
Gene ID : ENSG00000136450.12
Essential Gene : Yes

Functions

Splicing regulation
Viral RNA regulation

Localization(RBPImage: SRSF1)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Speckles
Cytoplasm
Cytosolic

Domains (AlphaFold: SRSF1)

RRM

Antibody Validation

View validation images for all SRSF1 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	SRSF1	ENSG00000136450.12	293.21	256.89	275.05	ENCSR000AEF
HepG2	SRSF1	ENSG00000136450.12	294.85	294.15	294.50	ENCSR985KAT
K562	SRSF1	ENSG00000136450.12	277.29	255.34	266.31	ENCSR000AEO

Assays

5 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 301	ENCSR989VIY	SRSF1	HepG2	Released	ENCAB962KAD	ENCSR499ZCU
eCLIP	eCLIP: 312	ENCSR432XUP	SRSF1	K562	Released	ENCAB712TFO	ENCSR925TYQ
eCLIP	eCLIP: 4045	ENCSR321PWZ	SRSF1	K562	Released	ENCAB916GNL	ENCSR961NOR
knockdown followed by RNA-seq	SRSF1-BGKLV11-K562	ENCSR066VOO	SRSF1	K562	Released		ENCSR129RWD	shRNA	KD% = 89.9,84.1
knockdown followed by RNA-seq	SRSF1-BGHLV12-HepG2	ENCSR094KBY	SRSF1	HepG2	Released		ENCSR603TCV	shRNA	KD% = 80.0,88.3

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
SRSF1	301	HepG2	ENCSR989VIY	ENCSR499ZCU	ENCAB962KAD	A302-052A	1	Released	ENCODE3
SRSF1	312	K562	ENCSR432XUP	ENCSR925TYQ	ENCAB712TFO	A302-052A	1	Released	ENCODE3
SRSF1	4045	K562	ENCSR321PWZ	ENCSR961NOR	ENCAB916GNL	32-4500	TG273235	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
SRSF1	ENCAB916GNL	32-4500	TG273235			Released, ENCODE4	BGHLV12-75, Released, ENCODE4
SRSF1	ENCAB962KAD	A302-052A	1		Released, ENCODE3	Released, ENCODE3	BGHLV12-75, Released, ENCODE3	BGKLV11-49, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
SRSF1	K562	shRNA	ENCSR066VOO	ENCSR129RWD	SRSF1-LV11-49,SRSF1-LV11-50	Nontarget-LV11-1,nontarget-LV11-2	89.9,84.1	Released	ENCODE3
SRSF1	HepG2	shRNA	ENCSR094KBY	ENCSR603TCV	SRSF1-BGHLV12-75,SRSF1-BGHLV12-76	NT-BGHLV12-93,NT-BGHLV12-94	80.0,88.3	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

SRSF1: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Speckles

Cytoplasm

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
SRSF1	Product_ID: A302-052A Lot_ID: 1 Source: Bethyl Labs Target Name: SRSF1-human
SRSF1	Product_ID: 32-4500 Lot_ID: TG273235 Source: Thermo Fisher Target Name: SRSF1-human

RNA Targets

SRSF1 protein binds to 3,810 RNA regions.

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Proteins bound to RNA

SRSF1 RNA is bound by 45 proteins.

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SRSF1 protein has 3,810 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
SRSF1	chr9	100777645	100777650	+	5.73369	400.00000	K562	ANP32B	CDS,intron-Proximal
SRSF1	chrX	73071754	73071768	-	4.39766	40.93945	K562	XIST	intron-Distal
SRSF1	chr12	76449898	76449920	-	3.75349	23.86223	K562	NAP1L1	3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal
SRSF1	chr20	42087071	42087085	+	4.46867	19.91934	HepG2	SRSF6	CDS
SRSF1	chr6	33239984	33240048	+	6.30301	18.53122	K562	RPS18	intron-Distal,intron-Proximal
SRSF1	chr9	131456285	131456297	+	5.05909	14.16163	HepG2	SET	CDS
SRSF1	chr11	67810197	67810271	+	4.22611	13.72367	K562	TCIRG1	CDS
SRSF1	chr12	124017715	124017849	-	3.74396	13.61401	HepG2	RILPL1	CDS,intron-Distal
SRSF1	chr12	11323852	11324005	-	4.36072	11.58574	HepG2	RP11-785H5.2, PRR4, TAS2R14	intron-Distal,intron-Proximal
SRSF1	chr20	61460124	61460155	+	3.44590	11.36772	HepG2	COL9A3	CDS

Showing 1 to 10 of 100 entries

45 proteins are bound to SRSF1 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr17	56080823	56080856	-	3'-UTR,intron-Distal,intron-Proximal	5.54804	400.00000	K562	CSTF2T
chr17	56082483	56082519	-	3'-UTR,5'-UTR,intron-Distal,intron-Proximal	4.35771	400.00000	K562	KHSRP
chr17	56082535	56082552	-	3'-UTR,5'-UTR,intron-Distal,intron-Proximal	4.41981	400.00000	HepG2	KHSRP
chr17	56082519	56082535	-	3'-UTR,5'-UTR,intron-Distal,intron-Proximal	4.46317	400.00000	K562	KHSRP
chr17	56082279	56082342	-	3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal	4.62930	400.00000	HepG2	BCLAF1
chr17	56080803	56080823	-	3'-UTR,intron-Distal,intron-Proximal	5.59694	400.00000	K562	CSTF2T
chr17	56081089	56081151	-	3'-UTR,intron-Distal,intron-Proximal	4.78217	400.00000	K562	FAM120A
chr17	56082535	56082552	-	3'-UTR,5'-UTR,intron-Distal,intron-Proximal	4.37115	400.00000	K562	KHSRP
chr17	56082487	56082523	-	3'-UTR,5'-UTR,intron-Distal,intron-Proximal	4.96127	400.00000	HepG2	KHSRP
chr17	56082416	56082465	-	3'-UTR,5'-UTR,intron-Distal,intron-Proximal	5.57308	400.00000	K562	SF3B4
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 226 entries

The SRSF1 protein binds to its own RNA.

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
SRSF1	chr17	56081309	56081363	-	3.54099	19.74314	HepG2	RP11-159D12.5, SRSF1	3'-UTR,intron-Distal
SRSF1	chr17	56081217	56081277	-	3.08837	10.41437	HepG2	RP11-159D12.5, SRSF1	3'-UTR,intron-Distal
SRSF1	chr17	56083074	56083132	-	4.77120	10.29660	K562	SRSF1	3'-UTR,CDS,intron-Distal,intron-Proximal
SRSF1	chr17	56082280	56082302	-	3.37838	9.13591	HepG2	SRSF1, RP11-159D12.5	3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal
SRSF1	chr17	56081277	56081299	-	3.30677	8.18487	HepG2	RP11-159D12.5, SRSF1	3'-UTR,intron-Distal

Showing 1 to 5 of 5 entries

Diffrential Expression

15,808 differentially expressed genes after Knockdown/Knockout SRSF1 protein.

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129 RBPs can affect the levels of SRSF1.

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15,808 differentially expressed genes after Knockdown/Knockout SRSF1 protein :

Only display 100 entries as example. Please click "View All" button to see all 15,808 entries.

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SLC7A2	ENSG00000003989.12	2143.25480	13857.05280	-2.69270	99999.99999	99999.99999	HepG2	SRSF1
TFPI	ENSG00000003436.10	5759.98070	21981.41060	-1.93210	270.11570	267.47730	HepG2	SRSF1
ABCB4	ENSG00000005471.11	311.64650	1219.00670	-1.96770	74.56070	72.87740	HepG2	SRSF1
ZNF195	ENSG00000005801.12	1416.13180	408.30640	1.79410	71.09470	69.44290	HepG2	SRSF1
CYP51A1	ENSG00000001630.11	5077.84490	13604.14690	-1.42180	63.28240	61.69660	HepG2	SRSF1
NFYA	ENSG00000001167.10	2111.17110	967.48210	1.12560	42.42580	41.05840	HepG2	SRSF1
CROT	ENSG00000005469.7	886.70760	309.88230	1.51660	38.04160	36.73490	HepG2	SRSF1
CDC27	ENSG00000004897.7	4753.15220	2701.11900	0.81520	36.41960	35.13400	HepG2	SRSF1
CDKL5	ENSG00000008086.6	344.99850	59.10150	2.54520	35.27640	34.00970	HepG2	SRSF1
TRAPPC6A	ENSG00000007255.6	51.24720	312.40670	-2.60790	34.61310	33.35260	HepG2	SRSF1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

129 RBPs can affect the gene expression of SRSF1 :

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Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SRSF1	ENSG00000136450.8	1292.41040	7496.09820	-2.53610	81.79360	78.31820	K562	SRSF1
SRSF1	ENSG00000136450.8	17545.48240	7565.53640	1.21350	34.92970	32.95420	K562	PUS1
SRSF1	ENSG00000136450.8	4427.42310	6846.21080	-0.62880	33.41960	32.06080	HepG2	ZNF622
SRSF1	ENSG00000136450.8	4158.79090	6485.70700	-0.64110	31.36220	29.67250	HepG2	CKAP4
SRSF1	ENSG00000136450.8	3088.18460	6687.55510	-1.11470	30.90080	29.16050	HepG2	RPLP0
SRSF1	ENSG00000136450.8	2573.37340	4292.34910	-0.73810	29.60250	28.42030	HepG2	SRSF1
SRSF1	ENSG00000136450.8	2997.32800	6291.58260	-1.06970	27.96240	26.39150	HepG2	BOP1
SRSF1	ENSG00000136450.8	3685.04770	6099.29350	-0.72700	28.00910	26.24850	HepG2	SRSF7
SRSF1	ENSG00000136450.8	5208.30210	8536.05350	-0.71280	25.00840	23.76410	HepG2	RBM22
SRSF1	ENSG00000136450.8	6352.35080	4094.21960	0.63360	23.44260	21.95460	HepG2	RECQL
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 163 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

5,940 alternative splicing events after Knockdown/Knockout SRSF1 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	CUL1	ENSG00000055130.11	chr7	+	148456395	148456446	148454059	148454242	148456639	148456730	0.10200	0.00001	included	K562	SRSF1
SE	PLEKHB2	ENSG00000115762.12	chr2	+	131901980	131902022	131897739	131897848	131904209	131905370	0.17600	0.00000	included	K562	SRSF1
SE	SPAG9	ENSG00000008294.16	chr17	-	49109041	49109083	49098518	49098726	49118861	49119012	0.32500	0.00021	included	K562	SRSF1
SE	AZI2	ENSG00000163512.9	chr3	-	28383002	28383087	28381892	28382113	28390091	28390281	0.08200	0.00004	included	K562	SRSF1
SE	EEF1E1-BLOC1S5	ENSG00000265818.1	chr6	-	8054502	8054605	8041371	8041501	8062766	8062849	0.15400	0.00003	included	K562	SRSF1
SE	YWHAZ	ENSG00000164924.13	chr8	-	101964156	101964356	101960962	101961128	101965496	101965569	0.22200	0.00000	included	K562	SRSF1
SE	KTN1	ENSG00000126777.13	chr14	+	56139889	56139973	56139646	56139730	56142552	56142636	0.34800	0.00000	included	K562	SRSF1
SE	KTN1	ENSG00000126777.13	chr14	+	56147403	56147459	56146282	56146403	56150816	56151297	0.14100	0.00000	included	K562	SRSF1
SE	KDM5B	ENSG00000117139.12	chr1	-	202735524	202735632	202733176	202733273	202736053	202736188	0.10900	0.00007	included	K562	SRSF1
SE	POLA1	ENSG00000101868.6	chrX	+	24873443	24873598	24861662	24861794	24906122	24906239	0.28100	0.00000	included	K562	SRSF1

Showing 1 to 10 of 100 entries

32 RBPs can affect the splicing of SRSF1 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082961	56081948	56082402	56083161	56083356	-0.11600	0.00000	excluded	K562	AQR
SE	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082961	56081948	56082402	56083161	56083334	-0.30300	0.00000	excluded	HepG2	TIA1
SE	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082961	56081948	56082402	56083161	56083334	-0.20600	0.00000	excluded	HepG2	PCBP2
SE	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082961	56081948	56082402	56083161	56083334	-0.23300	0.00000	excluded	HepG2	XPO5
SE	SRSF1	ENSG00000136450.8	chr17	-	56082758	56082961	56082283	56082402	56083161	56083334	-0.32500	0.00000	excluded	K562	HNRNPUL1
SE	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082961	56081948	56082402	56083161	56083334	-0.15000	0.00030	excluded	HepG2	SF3B4
SE	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082961	56081948	56082402	56083161	56083334	-0.33100	0.00000	excluded	HepG2	TAF15
SE	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082961	56081948	56082402	56083161	56083334	-0.23000	0.00019	excluded	HepG2	FUBP3
SE	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082961	56081948	56082402	56083161	56083334	-0.15900	0.00006	excluded	HepG2	SMNDC1
A3SS	SRSF1	ENSG00000136450.8	chr17	-	56082774	56082978	56082774	56082961	56083161	56083334	0.22700	0.00000	longer	K562	AGGF1

Showing 1 to 10 of 45 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

SRSF1

General Information

Functions

Localization(RBPImage: SRSF1)

Domains (AlphaFold: SRSF1)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

SRSF1 protein has 3,810 RNA targets:

45 proteins are bound to SRSF1 RNA:

The SRSF1 protein binds to its own RNA.

Diffrential Expression

15,808 differentially expressed genes after Knockdown/Knockout SRSF1 protein :

129 RBPs can affect the gene expression of SRSF1 :

5,940 alternative splicing events after Knockdown/Knockout SRSF1 protein :

32 RBPs can affect the splicing of SRSF1 :

No autoregularoty alternative splicing events between protein SRSF1 and its RNA.