BUD13

General Information

Gene Name : BUD13
Gene ID : ENSG00000137656.11
Essential Gene : Yes

Functions

Splicing regulation
Spliceosome
RNA localization
RNA export

Localization(RBPImage: BUD13)

Nuclei
Mutually Exclusive from Nucleolus
Speckles
Cytoplasm
Cytosolic

Domains (AlphaFold: BUD13)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A303-320A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	BUD13	ENSG00000137656.11	29.29	24.87	27.08	ENCSR000AEF
HepG2	BUD13	ENSG00000137656.11	20.74	24.06	22.40	ENCSR985KAT
K562	BUD13	ENSG00000137656.11	23.49	22.91	23.20	ENCSR000AEO

Assays

4 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 497	ENCSR830BSQ	BUD13	HepG2	Released	ENCAB673IND	ENCSR777PDA
eCLIP	eCLIP: 508	ENCSR663WES	BUD13	K562	Released	ENCAB673IND	ENCSR736LKF
knockdown followed by RNA-seq	BUD13-BGKLV29-K562	ENCSR267RHP	BUD13	K562	Released		ENCSR032YMP	shRNA	KD% = 82.7,85.7
knockdown followed by RNA-seq	BUD13-BGHLV30-HepG2	ENCSR382QKD	BUD13	HepG2	Released		ENCSR067GHD	shRNA	KD% = 88.6,72.4

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
BUD13	497	HepG2	ENCSR830BSQ	ENCSR777PDA	ENCAB673IND	A303-320A	1	Released	ENCODE3
BUD13	508	K562	ENCSR663WES	ENCSR736LKF	ENCAB673IND	A303-320A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
BUD13	ENCAB673IND	A303-320A	1		Released, ENCODE3	Released, ENCODE3	BGHLV30-17, Released, ENCODE3	BGKLV29-15, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
BUD13	K562	shRNA	ENCSR267RHP	ENCSR032YMP	BUD13_BGKLV29-15,BUD13_BGKLV29-16	NT_BGKLV29-1,NT_BGKLV29-2	82.7,85.7	Released	ENCODE3
BUD13	HepG2	shRNA	ENCSR382QKD	ENCSR067GHD	BUD13_BGHLV30-17,BUD13_BGHLV30-18	NT_BGHLV30-1,NT_BGHLV30-2	88.6,72.4	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

BUD13: Click to RBP Image Database

Nuclei

Mutually Exclusive from Nucleolus

Speckles

Cytoplasm

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
BUD13	Product_ID: A303-320A Lot_ID: 1 Source: Bethyl Labs Target Name: BUD13-human

RNA Targets

BUD13 protein binds to 12,580 RNA regions.

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Proteins bound to RNA

BUD13 RNA is bound by 2 proteins.

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BUD13 protein has 12,580 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
BUD13	chr10	101577010	101577113	+	3.08487	400.00000	HepG2	ABCC2	CDS,intron-Proximal
BUD13	chr17	74732198	74732257	-	3.56743	72.45796	HepG2	SRSF2	3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal
BUD13	chr1	43296420	43296486	+	6.11628	38.10506	K562	ERMAP	5'-UTR,CDS,intron-Distal,intron-Proximal
BUD13	chr16	29816104	29816194	+	3.81379	31.33894	K562	KIF22	3'-UTR,CDS,intron-Proximal
BUD13	chr5	176980381	176980471	-	3.51524	26.93141	K562	FAM193B	5'-UTR,intron-Distal,intron-Proximal
BUD13	chr17	40369413	40369495	-	3.67304	18.63671	K562	STAT5B	CDS,intron-Distal,intron-Proximal
BUD13	chr12	57033772	57033798	-	3.61491	18.21194	K562	ATP5B	CDS,intron-Distal,intron-Proximal
BUD13	chr22	50354717	50354779	+	3.76710	17.63786	HepG2	PIM3	CDS,intron-Proximal
BUD13	chr3	49456534	49456658	-	4.86869	16.71417	HepG2	AMT	3'-UTR,CDS,intron-Distal,intron-Proximal
BUD13	chr2	27354659	27354726	-	4.30710	16.34099	HepG2	PREB	3'-UTR,CDS,intron-Distal,intron-Proximal

Showing 1 to 10 of 100 entries

2 proteins are bound to BUD13 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr11	116633887	116633975	-	CDS,intron-Distal	3.32073	3.63681	K562	RBM15
chr11	116620470	116620589	-	intron-Distal	3.44119	3.35103	HepG2	QKI
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 2 of 2 entries

No autoregularoty interactions between protein BUD13 and its RNA.

Diffrential Expression

2,578 differentially expressed genes after Knockdown/Knockout BUD13 protein.

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51 RBPs can affect the levels of BUD13.

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2,578 differentially expressed genes after Knockdown/Knockout BUD13 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
CREB3L3	ENSG00000060566.9	1274.50180	606.16500	1.07210	20.31180	17.39630	HepG2	BUD13
CD74	ENSG00000019582.10	3228.09040	1955.00650	0.72340	13.26900	10.79840	HepG2	BUD13
CTSA	ENSG00000064601.12	7258.64910	4339.99450	0.74190	13.05330	10.60450	HepG2	BUD13
TGFBR3	ENSG00000069702.6	11691.98350	7537.89100	0.63320	12.63570	10.22240	HepG2	BUD13
NCAPD2	ENSG00000010292.8	2334.66300	3710.98480	-0.66860	12.24340	9.85850	HepG2	BUD13
IDS	ENSG00000010404.13	1405.77020	820.85830	0.77610	11.49710	9.16780	HepG2	BUD13
TIMP2	ENSG00000035862.8	2085.06330	1302.26680	0.67900	10.65840	8.40290	HepG2	BUD13
DEPDC1	ENSG00000024526.12	322.16340	622.02860	-0.94920	10.65040	8.39840	HepG2	BUD13
TUBG2	ENSG00000037042.8	1199.12950	736.14050	0.70380	8.64070	6.58980	HepG2	BUD13
HEBP1	ENSG00000013583.4	157.77950	323.15180	-1.03430	8.52730	6.48350	HepG2	BUD13
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

51 RBPs can affect the gene expression of BUD13 :

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Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
BUD13	ENSG00000137656.7	207.80940	839.10090	-2.01360	36.70120	32.61510	K562	BUD13
BUD13	ENSG00000137656.7	170.98290	487.96600	-1.51290	20.78370	17.81900	HepG2	BUD13
BUD13	ENSG00000137656.7	1756.88990	1069.17900	0.71640	9.90210	8.92210	K562	PUS1
BUD13	ENSG00000137656.7	306.01270	550.52660	-0.84720	6.74440	5.77220	HepG2	GPKOW
BUD13	ENSG00000137656.7	379.20790	582.41940	-0.61910	6.65450	5.74060	HepG2	SRSF5
BUD13	ENSG00000137656.7	834.23380	1153.98380	-0.46810	6.68520	5.62170	K562	UPF1
BUD13	ENSG00000137656.7	428.75290	684.07720	-0.67400	6.30620	5.33370	HepG2	RPLP0
BUD13	ENSG00000137656.7	1616.35560	1041.92500	0.63340	5.67490	4.85070	K562	RBM15
BUD13	ENSG00000137656.7	394.43870	589.87570	-0.58060	4.71390	3.96100	HepG2	PUF60
BUD13	ENSG00000137656.7	447.39840	623.88580	-0.47970	4.75100	3.87690	HepG2	SRSF7
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 56 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

205 alternative splicing events after Knockdown/Knockout BUD13 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	SEC31A	ENSG00000138674.12	chr4	-	83752089	83752128	83750152	83750211	83763292	83763634	0.24800	0.00871	included	HepG2	BUD13
SE	FOXM1	ENSG00000111206.8	chr12	-	2974520	2974565	2973848	2973918	2975558	2975687	0.17400	0.01874	included	HepG2	BUD13
SE	KIF13A	ENSG00000137177.14	chr6	-	17790102	17790141	17788006	17788106	17794479	17794626	0.38700	0.00009	included	HepG2	BUD13
SE	GPR126	ENSG00000112414.10	chr6	+	142762044	142762090	142759348	142759501	142764473	142767403	0.13200	0.02007	included	HepG2	BUD13
SE	TXNL4A	ENSG00000141759.10	chr18	-	77737597	77737701	77732874	77733856	77748239	77748593	0.06900	0.00871	included	HepG2	BUD13
SE	GUCD1	ENSG00000138867.12	chr22	-	24950916	24951104	24944884	24944969	24951582	24951827	-0.39600	0.01140	excluded	HepG2	BUD13
SE	CARM1	ENSG00000142453.7	chr19	+	11032050	11032119	11031725	11031803	11032290	11033448	-0.19500	0.00518	excluded	HepG2	BUD13
SE	SNRNP70	ENSG00000104852.10	chr19	+	49605370	49606844	49604529	49604728	49607890	49607992	0.09300	0.00000	included	K562	BUD13
SE	TSNAX	ENSG00000116918.9	chr1	+	231699210	231699375	231696873	231697001	231700273	231702270	0.06700	0.00139	included	K562	BUD13
SE	WNK1	ENSG00000060237.12	chr12	+	977031	978270	971248	971436	980430	980514	0.16100	0.00000	included	K562	BUD13

Showing 1 to 10 of 100 entries

2 RBPs can affect the splicing of BUD13 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	BUD13	ENSG00000137656.7	chr11	-	116642702	116642797			116640844	116640938	116643537	116643703	0.12300	0.00004	0.00071	included	K562	AQR
A3SS	BUD13	ENSG00000137656.7	chr11	-	116640844	116641028			116640844	116640938	116643537	116643703	0.06400	0.00021	0.00170	longer	K562	AQR
A3SS	BUD13	ENSG00000137656.7	chr11	-	116636077	116636185			116636077	116636162	116640844	116640972	0.07900	0.00241	0.01522	longer	K562	AQR
MXE	BUD13	ENSG00000137656.7	chr11	-	116628481	116628666	116628984	116629123	116627861	116627943	116629789	116629895	-0.12000	0.00291	0.04523	2included	K562	HNRNPUL1
RI	BUD13	ENSG00000137656.7	chr11	-	116633268	116633982			116633268	116633453	116633670	116633982	-0.06400	0.01260	0.06597	excluded	K562	AQR

Showing 1 to 5 of 5 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

BUD13

General Information

Functions

Localization(RBPImage: BUD13)

Domains (AlphaFold: BUD13)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

BUD13 protein has 12,580 RNA targets:

2 proteins are bound to BUD13 RNA:

No autoregularoty interactions between protein BUD13 and its RNA.

Diffrential Expression

2,578 differentially expressed genes after Knockdown/Knockout BUD13 protein :

51 RBPs can affect the gene expression of BUD13 :

205 alternative splicing events after Knockdown/Knockout BUD13 protein :

2 RBPs can affect the splicing of BUD13 :

No autoregularoty alternative splicing events between protein BUD13 and its RNA.