RACK1

General Information

Gene Name       : RACK1
Gene ID             : ENSG00000204628.11
Essential Gene : Yes

Functions

Localization(RBPImage: RACK1)

Domains (AlphaFold: RACK1)


Antibody Validation

 View validation images for all RACK1 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RACK1ENSG00000204628.111811.302778.462294.88ENCSR000AEF
HepG2RACK1ENSG00000204628.113496.282151.692823.99ENCSR985KAT
K562RACK1ENSG00000204628.113085.072577.932831.50ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq GNB2L1-BGHLV18-HepG2ENCSR116YMUGNB2L1HepG2ReleasedENCSR674KEK   shRNAKD% = 41.0,46.3






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
GNB2L1ENCAB611SPGA302-545A1Released, ENCODE3BGHLV18-11, Released, ENCODE3BGKLV21-13, Released, ENCODE3


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
GNB2L1K562shRNABGKLV21-13,BGKLV21-14BGKLV21-1,BGKLV21-241.5,70.3NotSatisfiedENCODE3
GNB2L1HepG2shRNAENCSR116YMUENCSR674KEKGNB2L1_BGHLV18_11,GNB2L1_BGHLV18_12NT_BGHLV18_1,NT_BGHLV18_241.0,46.3ReleasedENCODE3


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  
    GNB2L1: Click to RBP Image Database
  • Nuclei
  • Nucleoplasm
  • Mutually Exclusive from Nucleolus
  • Speckles
  • Cytoplasm
  • Compartment/Organelle
  • Unknown Cyto Foci
  • Cytosolic












    • RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
    RACK1Product_ID: 5432S
    Lot_ID: 2
    Source: Cell Signaling Technology
    Target Name: RACK1-human    		 RACK1-HEPG2-CRISPR-5432S.png<br>Caption: Western blot following CRISPR against RACK1 in HepG2 whole cell lysate using RACK1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RACK1. RACK1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
    		 CST_5432S_2_RACK1.png<br>Caption: IP-WB analysis of 5432S whole cell lysate using the RACK1 specific antibody, 5432S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RACK1-specific antibody, 5432S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
    		 RACK1-K562-CRISPR-5432S.png<br>Caption: Western blot following CRISPR against RACK1 in K562 whole cell lysate using RACK1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RACK1. RACK1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








    RNA Targets

    • eCLIP assay for RACK1 is not avaliable on ENCORE database.



    Proteins bound to RNA

    • NO known proteins bind on RACK1 RNA with finished eCLIP experiments on ENCORE database.





    eCLIP assay for RACK1 is not avaliable on ENCORE database.






    NO known proteins are bound to RACK1 RNA with finished eCLIP experiments on ENCORE database.





    eCLIP assay for RACK1 is not avaliable on ENCORE database.



    Diffrential Expression

    • The Knockdown/knockout assay for RACK1 is not available on ENCORE database.

    • No RBPs affect the gene expression of RACK1 on ENCORE database.


    The Knockdown/knockout RNA-Seq assay for RACK1 is not available on ENCORE database.






    No RBPs affect the gene expression of RACK1 on ENCORE database.






    The Knockdown/knockout RNA-Seq assay for RACK1 is not available on ENCORE database.






    No RBPs affect the alternative splcing events of RACK1 on ENCORE database.





    The Knockdown/knockout RNA-Seq assay for RACK1 is not avaliable on ENCORE database.