RACK1

General Information

Gene Name : RACK1
Gene ID : ENSG00000204628.11
Essential Gene : Yes

Functions

Translation regulation
Viral RNA regulation

Localization(RBPImage: RACK1)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Speckles
Cytoplasm
Compartment/Organelle
Unknown Cyto Foci
Cytosolic

Domains (AlphaFold: RACK1)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

View validation images for all RACK1 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	RACK1	ENSG00000204628.11	1811.30	2778.46	2294.88	ENCSR000AEF
HepG2	RACK1	ENSG00000204628.11	3496.28	2151.69	2823.99	ENCSR985KAT
K562	RACK1	ENSG00000204628.11	3085.07	2577.93	2831.50	ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	GNB2L1-BGHLV18-HepG2	ENCSR116YMU	GNB2L1	HepG2	Released		ENCSR674KEK	shRNA	KD% = 41.0,46.3

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
GNB2L1	ENCAB611SPG	A302-545A	1		Released, ENCODE3		BGHLV18-11, Released, ENCODE3	BGKLV21-13, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
GNB2L1	K562	shRNA			BGKLV21-13,BGKLV21-14	BGKLV21-1,BGKLV21-2	41.5,70.3	NotSatisfied	ENCODE3
GNB2L1	HepG2	shRNA	ENCSR116YMU	ENCSR674KEK	GNB2L1_BGHLV18_11,GNB2L1_BGHLV18_12	NT_BGHLV18_1,NT_BGHLV18_2	41.0,46.3	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

GNB2L1: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Speckles

Cytoplasm

Compartment/Organelle

Unknown Cyto Foci

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
RACK1	Product_ID: 5432S Lot_ID: 2 Source: Cell Signaling Technology Target Name: RACK1-human

Summary

RNA Targets

eCLIP assay for RACK1 is not avaliable on ENCORE database.

Proteins bound to RNA

NO known proteins bind on RACK1 RNA with finished eCLIP experiments on ENCORE database.

eCLIP assay for RACK1 is not avaliable on ENCORE database.

NO known proteins are bound to RACK1 RNA with finished eCLIP experiments on ENCORE database.

eCLIP assay for RACK1 is not avaliable on ENCORE database.

Summary

Diffrential Expression

The Knockdown/knockout assay for RACK1 is not available on ENCORE database.
No RBPs affect the gene expression of RACK1 on ENCORE database.

RACK1

General Information

Functions

Localization(RBPImage: RACK1)

Domains (AlphaFold: RACK1)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for RACK1 is not avaliable on ENCORE database.

NO known proteins are bound to RACK1 RNA with finished eCLIP experiments on ENCORE database.

eCLIP assay for RACK1 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for RACK1 is not available on ENCORE database.

No RBPs affect the gene expression of RACK1 on ENCORE database.

The Knockdown/knockout RNA-Seq assay for RACK1 is not available on ENCORE database.

No RBPs affect the alternative splcing events of RACK1 on ENCORE database.

The Knockdown/knockout RNA-Seq assay for RACK1 is not avaliable on ENCORE database.