SMG8

General Information

Gene Name       : SMG8
Gene ID             : ENSG00000167447.12
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: SMG8)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878SMG8ENSG00000167447.1211.9310.6211.27ENCSR000AEF
HepG2SMG8ENSG00000167447.1215.2022.0618.63ENCSR985KAT
K562SMG8ENSG00000167447.1212.9815.4614.22ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq SMG8-BGKcLV33-K562SMG8K562Released   CRISPRKD% = no band on the position,
knockdown followed by RNA-seq SMG8-BGKcLV33-K562ENCSR869YRRSMG8K562ReleasedENCSR271ZAB   CRISPRKD% = band isn't on the position,






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
SMG8ENCAB236BQWA301-471A1BGKcLV33-59, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
SMG8K562CRISPRBGKcLV33-55,BGKcLV33-56BGKcLV33-1,BGKcLV33-2no band on the position,ReleasedENCODE4
SMG8K562CRISPRENCSR869YRRENCSR271ZABSMG8-BGKcLV33-59,SMG8-BGKcLV33-60NT-BGKcLV33-1,NT-BGKcLV33-2band isn't on the position,ReleasedENCODE4
SMG8HepG2CRISPRBGHcLV31-55,BGHcLV31-56BGHcLV31-1,BGHcLV31-2NO CLEAR BAND,NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMG8Product_ID: A301-471A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SMG8-human		 SMG8-K562-CRISPR-A301-471A.png<br>Caption: Western blot following CRISPR against SMG8 in K562 whole cell lysate using SMG8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG8. SMG8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for SMG8 is not avaliable on ENCORE database.



Proteins bound to RNA
  • SMG8 RNA is bound by 5 proteins.  







eCLIP assay for SMG8 is not avaliable on ENCORE database.





5 proteins are bound to SMG8 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr175729240357292497+3'-UTR5.945079.27187HepG2FUBP3
chr175729016057290319+3'-UTR,CDS,intron-Distal,intron-Proximal3.107563.61537HepG2G3BP1
chr175729084257290949+CDS,intron-Distal4.020923.46958K562YBX3
chr175729072657290840+CDS,intron-Distal3.533783.27569HepG2BCLAF1
chr175728763657287751+CDS,intron-Distal,intron-Proximal3.436563.54074K562RBM15
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for SMG8 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for SMG8 is not available on ENCORE database.

  • 50 RBPs can affect the levels of SMG8.  








The Knockdown/knockout RNA-Seq assay for SMG8 is not available on ENCORE database.





50 RBPs can affect the gene expression of SMG8 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
SMG8ENSG00000167447.8846.90970658.740200.362403.841503.14680HepG2ACO1
SMG8ENSG00000167447.82321.455301083.771301.0989016.1284014.95320HepG2AKAP1
SMG8ENSG00000167447.81286.343401024.403300.328403.827003.04400HepG2AUH
SMG8ENSG00000167447.8931.89450465.715801.0006014.2512013.04090HepG2BOP1
SMG8ENSG00000167447.8802.11060658.523000.284502.597001.94830HepG2CKAP4
SMG8ENSG00000167447.8863.82110551.367700.647603.679602.79180HepG2CNOT7
SMG8ENSG00000167447.8457.43750595.01730-0.379402.858601.82300HepG2YBX3
SMG8ENSG00000167447.81132.84340939.491000.269902.618801.99150HepG2CELF1
SMG8ENSG00000167447.81374.638101038.161000.404902.884902.24780HepG2DDX3X
SMG8ENSG00000167447.8652.81580891.82510-0.450102.662602.05630HepG2DDX6
SMG8ENSG00000167447.8470.75040857.30990-0.864909.037208.00310HepG2EIF2S1
SMG8ENSG00000167447.8897.38540675.496600.409702.675101.91720HepG2FIP1L1
SMG8ENSG00000167447.8740.61750941.42730-0.346102.116401.34390HepG2G3BP2
SMG8ENSG00000167447.8759.29870628.863400.271802.255101.36730HepG2HDGF
SMG8ENSG00000167447.8524.93270651.77370-0.312202.387501.82120HepG2HNRNPU
SMG8ENSG00000167447.8644.84910812.58430-0.333602.642601.93450HepG2IGF2BP1
SMG8ENSG00000167447.8539.37140665.76550-0.303702.778702.04450HepG2ILF2
SMG8ENSG00000167447.8677.07710906.62660-0.421202.242501.35220HepG2KHSRP
SMG8ENSG00000167447.8823.448701001.60210-0.282602.086601.42220HepG2NOL12
SMG8ENSG00000167447.8423.33460581.33030-0.457603.926903.06430HepG2PPIL4
SMG8ENSG00000167447.8691.79090956.14450-0.466904.013803.31050HepG2PUF60
SMG8ENSG00000167447.8816.29470670.958800.282802.491501.89880HepG2PUS1
SMG8ENSG00000167447.8657.31900992.57250-0.594607.390006.44200HepG2QKI
SMG8ENSG00000167447.81476.17770983.007000.586507.034906.29820HepG2RBM22
SMG8ENSG00000167447.8745.830001019.97310-0.451605.262904.55520HepG2NELFE
SMG8ENSG00000167447.8705.58900949.98500-0.429104.810104.02630HepG2RECQL
SMG8ENSG00000167447.8455.33300580.43400-0.350202.266701.67420HepG2SAFB2
SMG8ENSG00000167447.8752.73810916.86010-0.284602.562001.88330HepG2SLBP
SMG8ENSG00000167447.8845.27990509.372600.730603.293002.43680HepG2SNRNP70
SMG8ENSG00000167447.81553.165701110.292900.484209.026508.26430HepG2SRFBP1
SMG8ENSG00000167447.81256.36530995.965400.335003.915403.37680HepG2SRSF1
SMG8ENSG00000167447.8648.54560810.75230-0.322103.079202.35110HepG2SRSF7
SMG8ENSG00000167447.8595.30160895.23760-0.588607.206706.37510HepG2TAF15
SMG8ENSG00000167447.8488.61130633.32430-0.374302.820702.31630HepG2TIAL1
SMG8ENSG00000167447.8512.60240638.98110-0.317902.673202.11640HepG2TRIM56
SMG8ENSG00000167447.8855.83140577.953000.566305.020904.18810HepG2U2AF1
SMG8ENSG00000167447.8267.26400409.29390-0.614903.857302.96200HepG2UCHL5
SMG8ENSG00000167447.8436.99610669.86850-0.616307.863106.78370HepG2UPF1
SMG8ENSG00000167447.8493.10820682.04390-0.468002.832602.09990HepG2XRN2
SMG8ENSG00000167447.83579.05930921.660401.9572072.2915069.67560K562AQR
SMG8ENSG00000167447.8572.44250453.925000.334602.156801.39410K562DKC1
SMG8ENSG00000167447.8755.81480388.815600.9588012.4942010.70550K562EIF4A3
SMG8ENSG00000167447.8888.72840404.349901.1360023.2043021.57010K562FXR1
SMG8ENSG00000167447.8579.50280437.361700.405901.913501.33750K562G3BP2
SMG8ENSG00000167447.8519.97750388.271800.421302.776601.54570K562NCBP2
SMG8ENSG00000167447.8645.93300518.623200.316602.077401.38320K562POLR2G
SMG8ENSG00000167447.81133.18660539.306001.0711015.8466014.03060K562PRPF8
SMG8ENSG00000167447.8780.13810592.356600.397203.255802.54120K562PTBP1
SMG8ENSG00000167447.81826.198101275.692100.517506.354605.11890K562PUF60
SMG8ENSG00000167447.8776.33080568.131200.450303.535202.57910K562RBM22
SMG8ENSG00000167447.8718.34000580.679900.306801.968501.35970K562SRSF1
SMG8ENSG00000167447.8743.32340520.203900.514804.306003.27480K562SYNCRIP
SMG8ENSG00000167447.8439.28020566.93040-0.368002.298501.55950K562TIA1
SMG8ENSG00000167447.8551.11610437.970700.331402.101701.40240K562U2AF2
SMG8ENSG00000167447.8397.75690552.65970-0.474502.842801.80660K562UCHL5
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for SMG8 is not available on ENCORE database.





1 RBPs can affect the splicing of SMG8 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
A3SSSMG8ENSG00000167447.8chr17+5728968257289847572897015728984757287260572891710.094000.000120.00104longerK562AQR
A3SSSMG8ENSG00000167447.8chr17+5729003057290217572900895729021757289701572898470.083000.001480.00989longerK562AQR
A5SSSMG8ENSG00000167447.8chr17+5728968257289930572896825728984757290089572909620.057000.002170.03624longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for SMG8 is not avaliable on ENCORE database.