ADD3

General Information

Gene Name : ADD3
Gene ID : ENSG00000148700.14
Essential Gene : No

Functions

Novel RBP

Localization

Not Available

Domains (AlphaFold: ADD3)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A303-715A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	ADD3	ENSG00000148700.14	125.58	87.49	106.53	ENCSR000AEF
HepG2	ADD3	ENSG00000148700.14	46.09	56.52	51.31	ENCSR985KAT
K562	ADD3	ENSG00000148700.14	38.58	64.33	51.45	ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	ADD3-BGHcLV25-HepG2	ENCSR558DZW	ADD3	HepG2	Released		ENCSR953VMB	CRISPR	KD% = 98.7,97.9

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
ADD3	ENCAB314ANI	A303-715A	1				BGHcLV25-13, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
ADD3	K562	CRISPR			BGKcLV26-9,BGKcLV26-10	BGKcLV26-1,BGKcLV26-2	no clear band on the position,	NotSatisfied	ENCODE4
ADD3	K562	CRISPR			BGKcLV46-3,BGKcLV46-4	BGKcLV46-1,BGKcLV46-2	0.0,0.0	NotSatisfied	ENCORE2
ADD3	K562	CRISPR			BGKcLV48-3,BGKcLV48-4	BGKcLV48-1,BGKcLV48-2	0.0,0.0	Experimented	ENCORE2
ADD3	K562	CRISPR			BGKcLV48-5,BGKcLV48-6	BGKcLV48-1,BGKcLV48-2	0.0,0.0	Experimented	ENCORE2
ADD3	HepG2	CRISPR	ENCSR558DZW	ENCSR953VMB	ADD3-BGHcLV25-13,ADD3-BGHcLV25-14	NT-BGHcLV25-1,NT-BGHcLV25-2	98.7,97.9	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
ADD3	Product_ID: A303-715A Lot_ID: 1 Source: Bethyl Labs Target Name: ADD3-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for ADD3 is not avaliable on ENCORE database.

Proteins bound to RNA

ADD3 RNA is bound by 21 proteins.

CanvasJS.com

eCLIP assay for ADD3 is not avaliable on ENCORE database.

21 proteins are bound to ADD3 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr10	111813381	111813472	+	intron-Distal	5.44283	11.15144	HepG2	QKI
chr10	111878341	111878396	+	CDS,intron-Distal,intron-Proximal	4.01376	10.98590	HepG2	BCLAF1
chr10	111881852	111881918	+	CDS,intron-Distal,intron-Proximal	4.71915	10.31463	HepG2	U2AF2
chr10	111885582	111885699	+	CDS,intron-Proximal	3.54504	9.97367	K562	UCHL5
chr10	111879291	111879327	+	CDS,intron-Distal,intron-Proximal	3.79646	9.74534	HepG2	BCLAF1
chr10	111893237	111893347	+	CDS	3.67162	9.72069	K562	UCHL5
chr10	111883949	111884029	+	CDS	4.02103	9.43617	K562	UCHL5
chr10	111893235	111893341	+	CDS	4.17163	9.37631	HepG2	BCLAF1
chr10	111800695	111800827	+	intron-Distal	5.39943	8.82480	HepG2	PTBP1
chr10	111885625	111885699	+	CDS,intron-Proximal	4.17916	8.79898	HepG2	BCLAF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 56 entries

eCLIP assay for ADD3 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for ADD3 is not available on ENCORE database.
113 RBPs can affect the levels of ADD3.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for ADD3 is not available on ENCORE database.

113 RBPs can affect the gene expression of ADD3 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
ADD3	ENSG00000148700.9	565.50180	1789.71510	-1.66210	91.97850	88.69040	HepG2	DNAJC2
ADD3	ENSG00000148700.9	915.33110	1870.02260	-1.03070	41.55500	39.69730	HepG2	CKAP4
ADD3	ENSG00000148700.9	982.71380	2260.17950	-1.20160	33.00220	30.59220	K562	SRSF1
ADD3	ENSG00000148700.9	831.69190	1704.87570	-1.03550	29.28150	27.45000	K562	FXR1
ADD3	ENSG00000148700.9	1149.09660	2064.39720	-0.84520	26.19020	24.63420	HepG2	UTP3
ADD3	ENSG00000148700.9	1812.34220	3758.66820	-1.05240	25.63880	23.09620	K562	PUF60
ADD3	ENSG00000148700.9	1727.02070	2611.53650	-0.59660	19.22580	17.59930	HepG2	DAZAP1
ADD3	ENSG00000148700.9	1355.51570	2057.55110	-0.60210	18.75400	17.03710	HepG2	ILF2
ADD3	ENSG00000148700.9	1236.11370	1974.77350	-0.67590	17.41150	16.15660	HepG2	TRIM56
ADD3	ENSG00000148700.9	1297.53910	2373.10650	-0.87100	18.11750	15.81050	K562	MAGOH
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 136 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for ADD3 is not available on ENCORE database.

34 RBPs can affect the splicing of ADD3 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.27800	0.00002	included	HepG2	RBM22
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.41100	0.00000	included	HepG2	HNRNPM
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.36700	0.00009	included	HepG2	TRA2A
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.70500	0.00000	included	HepG2	QKI
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.60000	0.00000	included	HepG2	DDX3X
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	-0.25700	0.00033	excluded	K562	HNRNPA1
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.33800	0.00001	included	K562	MTPAP
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.71800	0.00000	included	HepG2	NCBP2
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.39700	0.00005	included	K562	HNRNPM
SE	ADD3	ENSG00000148700.9	chr10	+	111892062	111892158	111890120	111890244	111893083	111893969	0.33500	0.00047	included	HepG2	GRSF1

Showing 1 to 10 of 45 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

ADD3

General Information

Functions

Localization

Domains (AlphaFold: ADD3)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for ADD3 is not avaliable on ENCORE database.

21 proteins are bound to ADD3 RNA:

eCLIP assay for ADD3 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for ADD3 is not available on ENCORE database.

113 RBPs can affect the gene expression of ADD3 :

The Knockdown/knockout RNA-Seq assay for ADD3 is not available on ENCORE database.

34 RBPs can affect the splicing of ADD3 :

The Knockdown/knockout RNA-Seq assay for ADD3 is not avaliable on ENCORE database.