WRN

General Information

Gene Name : WRN
Gene ID : ENSG00000165392.9
Essential Gene : No

Functions

Novel RBP

Localization(RBPImage: WRN)

Nuclei
Mutually Exclusive from Nucleolus
Speckles
Variable Nuclear Intensity
Cytoplasm
Cytosolic
Nuclear release mitosis

Domains (AlphaFold: WRN)

Helicase
Nuclease

Antibody Validation

GeneTex: GTX101081

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	WRN	ENSG00000165392.9	19.94	16.71	18.33	ENCSR000AEF
HepG2	WRN	ENSG00000165392.9	5.39	10.35	7.87	ENCSR985KAT
K562	WRN	ENSG00000165392.9	28.12	27.87	28.00	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 416	ENCSR038JME	WRN	K562	Released	ENCAB362RRM	ENCSR925ZRV
knockdown followed by RNA-seq	WRN-BGKLV38-K562	ENCSR165BCF	WRN	K562	Released		ENCSR718EWL	shRNA	KD% = 71.2,80.4
knockdown followed by RNA-seq	WRN-BGHLV37-HepG2	ENCSR093FHC	WRN	HepG2	Released		ENCSR225PRV	shRNA	KD% = 98.1,89.6

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
WRN	416	K562	ENCSR038JME	ENCSR925ZRV	ENCAB362RRM	GTX101081	39812	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
WRN	ENCAB362RRM	GTX101081	39812			Released, ENCODE3	BGHLV37-87, Released, ENCODE3	BGKLV38-109, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
WRN	K562	shRNA	ENCSR165BCF	ENCSR718EWL	WRN_BGKLV38-109,WRN_BGKLV38-110	NT_BGKLV38-1,NT_BGKLV38-2	71.2,80.4	Released	ENCODE3
WRN	K562	shRNA			BGKLV38-111,BGKLV38-112	BGKLV38-1,BGKLV38-2	43.5,17.8	NotSatisfied	ENCODE3
WRN	K562	shRNA			BGKLV38-113,BGKLV38-114	BGKLV38-1,BGKLV38-2	72.1,67.7	NotSatisfied	ENCODE3
WRN	HepG2	shRNA			BGHLV33-41,BGHLV33-42	BGHLV33-1,BGHLV33-2	0.0,37.0	NotSatisfied	ENCODE3
WRN	HepG2	shRNA	ENCSR093FHC	ENCSR225PRV	WRN-BGHLV37-87C,WRN-BGHLV37-88C	non-target-BGHLV37-1C,non-target-BGHLV37-2C	98.1,89.6	Released	ENCODE4
WRN	HepG2	shRNA			BGHLV37-89,BGHLV37-90	BGHLV37-1,BGHLV37-2	88.0,76.9	NotSatisfied	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

WRN: Click to RBP Image Database

Nuclei

Mutually Exclusive from Nucleolus

Speckles

Variable Nuclear Intensity

Cytoplasm

Cytosolic

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
WRN	Product_ID: GTX101081 Lot_ID: 39812 Source: GeneTex Target Name: WRN-human

RNA Targets

WRN protein binds to 105 RNA regions.

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Proteins bound to RNA

WRN RNA is bound by 32 proteins.

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WRN protein has 105 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
WRN	chr19	45981871	45981875	-	6.01448	400.00000	K562	ERCC1	intron-Distal
WRN	chr13	41634901	41634936	-	4.17672	400.00000	K562	ELF1	intron-Distal
WRN	chr3	45730491	45730527	-	3.01301	400.00000	K562	LIMD1-AS1	intron-Distal
WRN	chr19	45981875	45981898	-	5.28655	400.00000	K562	ERCC1	intron-Distal
WRN	chr13	41634860	41634880	-	3.59528	400.00000	K562	ELF1	intron-Distal
WRN	chr2	27273734	27273743	+	3.00087	400.00000	K562	AGBL5	intron-Distal
WRN	chr19	45981854	45981857	-	6.10909	400.00000	K562	ERCC1	intron-Distal
WRN	chr19	45981857	45981871	-	6.01448	400.00000	K562	ERCC1	intron-Distal
WRN	chr13	41634880	41634901	-	3.66861	400.00000	K562	ELF1	intron-Distal
WRN	chr3	45730478	45730489	-	3.09870	400.00000	K562	LIMD1-AS1	intron-Distal

Showing 1 to 10 of 100 entries

32 proteins are bound to WRN RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr8	30976707	30976719	+	intron-Distal	5.74676	68.23125	K562	PTBP1
chr8	30976650	30976707	+	intron-Distal	4.95318	62.81887	K562	PTBP1
chr8	30976719	30976754	+	intron-Distal	6.90538	60.20258	K562	PTBP1
chr8	30976705	30976749	+	intron-Distal	7.28678	36.96902	HepG2	PTBP1
chr8	30976628	30976754	+	intron-Distal	4.68775	29.48948	K562	MATR3
chr8	30976635	30976705	+	intron-Distal	5.48057	29.30575	HepG2	PTBP1
chr8	30938588	30938718	+	CDS	3.93309	28.89493	K562	UCHL5
chr8	30938686	30938766	+	CDS	4.13681	24.52185	K562	ZNF622
chr8	30976840	30976906	+	intron-Distal	5.09947	23.82345	K562	PTBP1
chr8	30945373	30945432	+	CDS	4.94192	23.17584	K562	ZNF622
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 167 entries

No autoregularoty interactions between protein WRN and its RNA.

Diffrential Expression

1,510 differentially expressed genes after Knockdown/Knockout WRN protein.

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71 RBPs can affect the levels of WRN.

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1,510 differentially expressed genes after Knockdown/Knockout WRN protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
ABHD5	ENSG00000011198.3	3843.01710	1893.78300	1.02090	27.21590	23.84730	K562	WRN
HDAC7	ENSG00000061273.13	2894.52990	5550.31660	-0.93920	24.46240	21.30770	K562	WRN
SLC4A1	ENSG00000004939.9	2439.15290	405.62580	2.58810	17.79000	15.01350	K562	WRN
ATP2B4	ENSG00000058668.10	1319.76890	2320.30810	-0.81400	15.30360	12.69980	K562	WRN
SLC2A3	ENSG00000059804.11	6076.92740	10208.68530	-0.74840	13.36450	10.84660	K562	WRN
PTPRN	ENSG00000054356.9	189.18520	59.02870	1.68020	12.06230	9.58680	K562	WRN
ABCA7	ENSG00000064687.8	2650.63020	4146.93540	-0.64570	11.57190	9.13960	K562	WRN
PRKAR2B	ENSG00000005249.8	7547.80360	11051.31080	-0.55010	10.06790	7.75140	K562	WRN
FAM13B	ENSG00000031003.6	1748.45010	1127.59180	0.63270	9.16280	6.90200	K562	WRN
TKTL1	ENSG00000007350.12	161.80310	56.95100	1.50630	9.12300	6.86530	K562	WRN
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

71 RBPs can affect the gene expression of WRN :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
WRN	ENSG00000165392.5	203.55320	523.44330	-1.36260	20.47030	19.34190	HepG2	TIAL1
WRN	ENSG00000165392.5	1650.17720	2895.69500	-0.81130	20.15690	18.47960	K562	EIF2S2
WRN	ENSG00000165392.5	957.60820	504.71840	0.92390	16.69090	15.48040	HepG2	TAF15
WRN	ENSG00000165392.5	1455.79060	922.41410	0.65820	14.24760	13.34620	HepG2	SRFBP1
WRN	ENSG00000165392.5	368.08380	725.26620	-0.97850	12.74850	11.59430	HepG2	PUF60
WRN	ENSG00000165392.5	1132.93080	2048.34940	-0.85440	13.13090	11.48760	K562	PRPF8
WRN	ENSG00000165392.5	250.47560	542.32030	-1.11450	12.22950	10.86130	HepG2	HNRNPC
WRN	ENSG00000165392.5	361.08760	628.22680	-0.79890	11.91740	10.79250	HepG2	PUS1
WRN	ENSG00000165392.5	1100.46510	1815.95390	-0.72260	11.73350	10.57850	K562	HNRNPC
WRN	ENSG00000165392.5	4080.45570	5831.43740	-0.51510	12.45890	10.43250	K562	PUM2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 79 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

19 alternative splicing events after Knockdown/Knockout WRN protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	RPS24	ENSG00000138326.14	chr10	+	79799961	79799983			79796951	79797062	79800372	79800455	0.08700	0.00000	0.00000	included	K562	WRN
SE	NSFL1C	ENSG00000088833.13	chr20	-	1443979	1444086			1438844	1438919	1444973	1445071	0.07300	0.00000	0.00011	included	K562	WRN
SE	SRRM1	ENSG00000133226.12	chr1	+	24973569	24973699			24973117	24973280	24975349	24975520	0.14900	0.00000	0.00049	included	K562	WRN
SE	RPLP0	ENSG00000089157.11	chr12	-	120636656	120636704			120635124	120635265	120636920	120637008	0.09400	0.00000	0.00007	included	K562	WRN
SE	CDK10	ENSG00000185324.17	chr16	+	89755659	89755732			89753113	89753167	89756960	89757032	-0.12600	0.00000	0.02016	excluded	K562	WRN
SE	SRRM2	ENSG00000167978.12	chr16	+	2818505	2818600			2817722	2818262	2818997	2819249	-0.07700	0.00000	0.00193	excluded	K562	WRN
MXE	PPP2R5C	ENSG00000078304.15	chr14	+	102252354	102252520	102276249	102276373	102229241	102229307	102323022	102323222	0.05400	0.00000	0.00461	1included	K562	WRN
SE	MYB	ENSG00000118513.14	chr6	+	135520663	135520719			135520045	135520188	135521222	135521337	0.05700	0.00001	0.04178	included	K562	WRN
SE	LYRM5	ENSG00000205707.6	chr12	+	25356658	25356766			25348205	25348271	25356853	25356941	0.06500	0.00001	0.04171	included	K562	WRN
SE	RPL27	ENSG00000131469.8	chr17	+	41150765	41150842			41150452	41150488	41151949	41152119	0.09000	0.00001	0.02846	included	K562	WRN

Showing 1 to 10 of 19 entries

11 RBPs can affect the splicing of WRN :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	WRN	ENSG00000165392.5	chr8	+	30947980	30948048	30946405	30946481	30948349	30948458	-0.30600	0.00000	0.00000	excluded	K562	SF3B4
A3SS	WRN	ENSG00000165392.5	chr8	+	30969115	30969315	30969130	30969315	30958364	30958471	0.11200	0.00000	0.00000	longer	K562	AQR
A3SS	WRN	ENSG00000165392.5	chr8	+	30916610	30916781	30916668	30916781	30915898	30916059	0.20800	0.00000	0.00157	longer	K562	UTP18
SE	WRN	ENSG00000165392.5	chr8	+	30947980	30948048	30946405	30946481	30948349	30948458	-0.19200	0.00001	0.00035	excluded	K562	U2AF1
SE	WRN	ENSG00000165392.5	chr8	+	30957995	30958068	30954283	30954366	30958364	30958471	0.10200	0.00001	0.00091	included	K562	SF3B1
SE	WRN	ENSG00000165392.5	chr8	+	31023591	31023682	31014883	31015046	31024537	31024746	0.06600	0.00001	0.00057	included	K562	PTBP1
A3SS	WRN	ENSG00000165392.5	chr8	+	30916610	30916781	30916668	30916781	30915887	30916059	0.21000	0.00001	0.00050	longer	K562	PUS1
SE	WRN	ENSG00000165392.5	chr8	+	30947980	30948048	30946405	30946481	30948349	30948458	-0.33400	0.00002	0.00062	excluded	K562	HNRNPC
SE	WRN	ENSG00000165392.5	chr8	+	30947980	30948048	30946405	30946481	30948349	30948458	-0.25200	0.00004	0.00122	excluded	K562	U2AF2
A3SS	WRN	ENSG00000165392.5	chr8	+	30947958	30948048	30947980	30948048	30946239	30946481	0.14600	0.00004	0.00035	longer	K562	AQR

Showing 1 to 10 of 19 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

WRN

General Information

Functions

Localization(RBPImage: WRN)

Domains (AlphaFold: WRN)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

WRN protein has 105 RNA targets:

32 proteins are bound to WRN RNA:

No autoregularoty interactions between protein WRN and its RNA.

Diffrential Expression

1,510 differentially expressed genes after Knockdown/Knockout WRN protein :

71 RBPs can affect the gene expression of WRN :

19 alternative splicing events after Knockdown/Knockout WRN protein :

11 RBPs can affect the splicing of WRN :

No autoregularoty alternative splicing events between protein WRN and its RNA.