RPUSD2

General Information

Gene Name       : RPUSD2
Gene ID             : ENSG00000166133.17
Essential Gene : No

Functions

Localization

Domains (AlphaFold: RPUSD2)


Antibody Validation

 View validation images for all RPUSD2 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RPUSD2ENSG00000166133.175.378.616.99ENCSR000AEF
HepG2RPUSD2ENSG00000166133.1712.907.6910.29ENCSR985KAT
K562RPUSD2ENSG00000166133.179.929.169.54ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RPUSD2-BGKcLV33-K562RPUSD2K562Released   CRISPRKD% = 88.7,85.3
knockdown followed by RNA-seq RPUSD2-BGKcLV33-K562ENCSR563QCQRPUSD2K562ReleasedENCSR271ZAB   CRISPRKD% = 0.0,0.0
knockdown followed by RNA-seq RPUSD2-BGHcLV31-HepG2ENCSR235JSARPUSD2HepG2ReleasedENCSR452TFV   CRISPRKD% = 83.9,81.2






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RPUSD2ENCAB305MHJA305-209A1Released, ENCODE4BGHcLV31-13, Released, ENCODE4BGKcLV33-17, Released, ENCODE4
RPUSD2ENCAB939JYDA305-210A1Released, ENCODE4BGHcLV31-13, Released, ENCODE4BGKcLV33-17, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RPUSD2K562CRISPRBGKcLV33-9,BGKcLV33-10BGKcLV33-1,BGKcLV33-288.7,85.3ReleasedENCODE4
RPUSD2K562CRISPRENCSR563QCQENCSR271ZABRPUSD2-BGKcLV33-17,RPUSD2-BGKcLV33-18NT-BGKcLV33-1,NT-BGKcLV33-20.0,0.0ReleasedENCODE4
RPUSD2HepG2CRISPRENCSR235JSAENCSR452TFVRPUSD2-BGHcLV31-13,RPUSD2-BGHcLV31-14NT-BGHcLV31-1,NT-BGHcLV31-283.9,81.2ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPUSD2Product_ID: A305-209A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPUSD2-human		 RPUSD2-HEPG2-CRISPR-A305-209A.png<br>Caption: Western blot following CRISPR against RPUSD2 in HepG2 whole cell lysate using RPUSD2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPUSD2. RPUSD2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A305-209A_1_RPUSD2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPUSD2 specific antibody, A305-209A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RPUSD2-specific antibody, A305-209A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RPUSD2-K562-CRISPR-A305-209A.png<br>Caption: Western blot following CRISPR against RPUSD2 in K562 whole cell lysate using RPUSD2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPUSD2. RPUSD2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RPUSD2Product_ID: A305-210A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPUSD2-human		 Bethyl_A305-210A_1_RPUSD2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RPUSD2 specific antibody, A305-210A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RPUSD2-specific antibody, A305-210A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RPUSD2-K562-CRISPR-A305-210A.png<br>Caption: Western blot following CRISPR against RPUSD2 in K562 whole cell lysate using RPUSD2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPUSD2. RPUSD2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for RPUSD2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • RPUSD2 RNA is bound by 1 proteins.  







eCLIP assay for RPUSD2 is not avaliable on ENCORE database.





1 proteins are bound to RPUSD2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr154086153940861637+CDS5.194925.82881K562DDX3X
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RPUSD2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RPUSD2 is not available on ENCORE database.

  • 37 RBPs can affect the levels of RPUSD2.  








The Knockdown/knockout RNA-Seq assay for RPUSD2 is not available on ENCORE database.





37 RBPs can affect the gene expression of RPUSD2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RPUSD2ENSG00000166133.13373.32200221.290100.754404.111503.48060HepG2AKAP1
RPUSD2ENSG00000166133.13276.36130183.103100.593802.751201.96890HepG2ASCC1
RPUSD2ENSG00000166133.13294.39420211.988100.473701.970501.43430HepG2DDX3X
RPUSD2ENSG00000166133.13113.45200175.05990-0.625802.118101.59150HepG2EIF2S1
RPUSD2ENSG00000166133.13265.92850351.63870-0.403102.350201.78840HepG2ETF1
RPUSD2ENSG00000166133.13268.57200185.054300.537302.999802.37160HepG2PCBP1
RPUSD2ENSG00000166133.13116.91920221.88750-0.924305.260104.35840HepG2RPLP0
RPUSD2ENSG00000166133.1377.42850126.03950-0.702902.142201.44300HepG2RRP9
RPUSD2ENSG00000166133.13238.04130331.93570-0.479703.100302.58020HepG2SRFBP1
RPUSD2ENSG00000166133.13270.46960204.627900.402401.748501.34800HepG2SRSF1
RPUSD2ENSG00000166133.13106.72710164.49100-0.624102.123601.52060HepG2SUPV3L1
RPUSD2ENSG00000166133.1354.3096097.15800-0.839102.665701.92000HepG2UCHL5
RPUSD2ENSG00000166133.13245.69090167.547900.552202.930502.31730HepG2UTP3
RPUSD2ENSG00000166133.13995.34400498.684400.997005.406604.63330K562AQR
RPUSD2ENSG00000166133.13556.22430386.636500.524604.514803.70930K562DDX47
RPUSD2ENSG00000166133.13313.22520455.51310-0.540304.399303.51990K562EIF2S2
RPUSD2ENSG00000166133.13267.29590143.554500.896706.563605.35340K562EIF3G
RPUSD2ENSG00000166133.13330.74600163.092501.019908.316506.89420K562EIF4A3
RPUSD2ENSG00000166133.13391.66830169.606201.2073013.6395012.39890K562FXR1
RPUSD2ENSG00000166133.1344.4162088.58750-0.996002.799302.08620K562G3BP2
RPUSD2ENSG00000166133.13117.74550212.05570-0.848802.584901.41710K562HNRNPA2B1
RPUSD2ENSG00000166133.13303.16420436.56300-0.526103.117302.27470K562HSPD1
RPUSD2ENSG00000166133.13305.13990162.560500.908407.219605.83560K562MAGOH
RPUSD2ENSG00000166133.13262.88120162.205400.696502.437001.57700K562MATR3
RPUSD2ENSG00000166133.13274.98530360.76780-0.391702.451801.74510K562NSUN2
RPUSD2ENSG00000166133.13331.20280157.950201.068109.786108.59290K562PTBP1
RPUSD2ENSG00000166133.13710.92560574.897500.306302.083101.37510K562PUF60
RPUSD2ENSG00000166133.13121.02400187.36220-0.630502.349901.84110K562PUS1
RPUSD2ENSG00000166133.13226.11720151.364300.578902.741701.90560K562RBM22
RPUSD2ENSG00000166133.13276.36780159.308000.794702.689701.61380K562NELFE
RPUSD2ENSG00000166133.13119.0077063.467000.906902.441101.50740K562RPS3
RPUSD2ENSG00000166133.13286.56450154.862500.887806.176205.12980K562SRSF1
RPUSD2ENSG00000166133.13227.54720386.40750-0.764005.238103.43430K562STAU1
RPUSD2ENSG00000166133.13323.74320210.130400.623503.993303.00320K562SYNCRIP
RPUSD2ENSG00000166133.13234.79290156.690700.583402.712102.01100K562TARDBP
RPUSD2ENSG00000166133.13364.36100483.44910-0.408002.842601.55460K562TROVE2
RPUSD2ENSG00000166133.13123.04830272.33100-1.146107.891006.09930K562UCHL5
RPUSD2ENSG00000166133.13298.83830382.53000-0.356202.139301.50150K562UPF1
RPUSD2ENSG00000166133.13172.33540327.31850-0.925507.527306.04160K562WDR3
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RPUSD2 is not available on ENCORE database.






No RBPs affect the alternative splcing events of RPUSD2 on ENCORE database.





The Knockdown/knockout RNA-Seq assay for RPUSD2 is not avaliable on ENCORE database.