EIF5B

General Information

Gene Name : EIF5B
Gene ID : ENSG00000158417.10
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: EIF5B)

Not Available

Antibody Validation

Bethyl Labs: A301-745A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	EIF5B	ENSG00000158417.10	90.19	81.02	85.60	ENCSR000AEF
HepG2	EIF5B	ENSG00000158417.10	45.06	89.66	67.36	ENCSR985KAT
K562	EIF5B	ENSG00000158417.10	87.44	95.90	91.67	ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
EIF5B		A301-745A	1					BGKcLV44-25, Submitted, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Sample_reps	Control_reps	KD%	Status	Project
EIF5B	K562	CRISPR	BGKcLV40-19,BGKcLV40-20	BGKcLV40-1,BGKcLV40-2	not enough cells,	NotSatisfied	ENCORE2
EIF5B	K562	CRISPR	EIF5B-BGKcLV44-25,EIF5B-BGKcLV44-26	NT-BGKcLV44-1,NT-BGKcLV44-2	69.2,67.4	Submitting	ENCORE2
EIF5B	HepG2	CRISPR	EIF5B-BGHcLV41-39,EIF5B-BGHcLV41-40	NT-BGHcLV41-1,NT-BGHcLV41-2	too many bands,	Submitting	ENCORE2
EIF5B	HepG2	CRISPR	EIF5B-BGHcLV45-29,EIF5B-BGHcLV45-30	NT-BGHcLV45-1,NT-BGHcLV45-2	73.1,77.8	Submitting	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
EIF5B	Product_ID: A301-745A Lot_ID: 1 Source: Bethyl Labs Target Name: EIF5B-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for EIF5B is not avaliable on ENCORE database.

Proteins bound to RNA

EIF5B RNA is bound by 33 proteins.

CanvasJS.com

eCLIP assay for EIF5B is not avaliable on ENCORE database.

33 proteins are bound to EIF5B RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr2	99992911	99993011	+	CDS	6.31097	77.27328	K562	UCHL5
chr2	99953941	99954051	+	5'-UTR,CDS	6.68120	72.34591	K562	UCHL5
chr2	99978117	99978277	+	CDS	7.72117	53.15288	K562	UCHL5
chr2	99978126	99978264	+	CDS	5.83606	38.38456	HepG2	BCLAF1
chr2	99992885	99993007	+	CDS	6.29473	36.39242	K562	ZNF622
chr2	99980209	99980270	+	CDS	6.29520	36.01611	K562	UCHL5
chr2	99992901	99993008	+	CDS	5.10209	33.34922	K562	TRA2A
chr2	99992895	99993018	+	CDS	4.45354	31.32209	K562	CPSF6
chr2	99992853	99992911	+	CDS	6.86896	30.48581	K562	UCHL5
chr2	99980149	99980209	+	CDS	6.07167	29.62248	K562	UCHL5
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 236 entries

eCLIP assay for EIF5B is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for EIF5B is not available on ENCORE database.
113 RBPs can affect the levels of EIF5B.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for EIF5B is not available on ENCORE database.

113 RBPs can affect the gene expression of EIF5B :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
EIF5B	ENSG00000158417.6	1763.03000	5286.31240	-1.58420	162.26820	159.94320	HepG2	ZNF622
EIF5B	ENSG00000158417.6	1783.82070	4640.56130	-1.37930	91.97730	89.29900	HepG2	UTP3
EIF5B	ENSG00000158417.6	4462.96130	10077.24990	-1.17500	65.19230	62.16550	K562	SF3A3
EIF5B	ENSG00000158417.6	7110.89520	3988.26920	0.83420	29.85180	28.66390	HepG2	SRSF1
EIF5B	ENSG00000158417.6	7265.87540	10212.85580	-0.49120	29.45070	27.43890	K562	NSUN2
EIF5B	ENSG00000158417.6	6934.87780	10828.89510	-0.64290	28.68660	26.68540	K562	UPF1
EIF5B	ENSG00000158417.6	3374.81750	8009.48250	-1.24690	23.50900	21.79510	HepG2	TARDBP
EIF5B	ENSG00000158417.6	6110.37980	9191.60590	-0.58910	23.47850	21.46390	K562	MTPAP
EIF5B	ENSG00000158417.6	7052.83720	4495.40480	0.64970	20.80050	19.25410	K562	FXR1
EIF5B	ENSG00000158417.6	2121.23860	3069.30010	-0.53300	16.91700	15.43830	HepG2	DNAJC2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 137 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for EIF5B is not available on ENCORE database.

3 RBPs can affect the splicing of EIF5B :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
A3SS	EIF5B	ENSG00000158417.6	chr2	+	99976669	99976824			99976698	99976824	99953815	99954052	0.05700	0.00000	0.00000	longer	K562	AQR
MXE	EIF5B	ENSG00000158417.6	chr2	+	99984896	99985054	99988118	99988193	99980733	99980884	99992809	99993099	0.05300	0.00000	0.00000	1included	K562	AQR
MXE	EIF5B	ENSG00000158417.6	chr2	+	99985854	99985944	99988118	99988193	99984955	99985054	99992809	99993099	-0.11000	0.00159	0.06981	2included	K562	TIA1
MXE	EIF5B	ENSG00000158417.6	chr2	+	99985854	99985944	99988118	99988193	99984955	99985054	99992809	99993099	0.09600	0.00634	0.07560	1included	K562	U2AF1

Showing 1 to 4 of 4 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

EIF5B

General Information

Functions

Localization

Domains (AlphaFold: EIF5B)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for EIF5B is not avaliable on ENCORE database.

33 proteins are bound to EIF5B RNA:

eCLIP assay for EIF5B is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for EIF5B is not available on ENCORE database.

113 RBPs can affect the gene expression of EIF5B :

The Knockdown/knockout RNA-Seq assay for EIF5B is not available on ENCORE database.

3 RBPs can affect the splicing of EIF5B :

The Knockdown/knockout RNA-Seq assay for EIF5B is not avaliable on ENCORE database.