EIF5B      CRISPR in HepG2      Control: NT-BGHcLV41-1,NT-BGHcLV41-2

General Information
RBPEIF5B
Cell_LineHepG2
MethodCRISPR
Exp_NameEIF5B-BGHcLV41-HepG2
ENCODE_series_ID
Batch_IDBGHcLV41
Pool IDPool-240314
Local_Set_Nameset70
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF5B-BGHcLV41-39
Rep2EIF5B-BGHcLV41-40
CN1NT-BGHcLV41-1
CN2NT-BGHcLV41-2
Rep1_qPCR31.5
Rep2_qPCR55.6
Rep1_WBtoo many bands
Rep2_WB
Antibody Cat#A301-745A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1453




Experiment Information (Status: Submitting)
BGHcLV41-39BGHcLV41-40
idx13081309
TRCN#_or_BGC#BGC#0000985BGC#0000985
shRNA_or_gRNA_sequenceAGCGCCATTGACAAGCAATGAGCGCCATTGACAAGCAATG
PAMGGGGGG
NameEIF5B_81EIF5B_81
Sample_IDBGHcLV41-39BGHcLV41-40
transduction_Date1/12/241/12/24
daysD6D6
RBP_nameEIF5BEIF5B
qPCR_result31.555.6
Ave_qPCR43.6
RT-qPCR_primer-Fagagagaagccagagccaga
RT-qPCR_primer-Rtttgatggcacttcaacacc
protein_conc30793421
WB_resulttoo many bands
Ave_WB
WB_DONE_date2/1/24
MW139kd
IP
antibody_Cat#A301-745Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Notecheck result befor release check result befor release
ID95519552




Western Blot
Western Blot info was not avaliable




Experiment Status
BGHcLV41-39BGHcLV41-40
Sample_IDBGHcLV41-39BGHcLV41-40
Sample Name
Sample NameEIF5B-BGHcLV41-39EIF5B-BGHcLV41-40
RBPEIF5BEIF5B
Cell_LineHepG2HepG2
Exp UID
StatusSubmittingSubmitting
Status_date2024-09-202024-09-20
ProjectENCORE2ENCORE2
Note
ID1296612967




Library-Prep Information
BGHcLV41-39BGHcLV41-40
Sample #1920
Sample NameEIF5B-BGHcLV41-39EIF5B-BGHcLV41-40
Sample_Name_Alias
Index Well PositionC03D03
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-03-012024-03-01
Lib_IDLib-240301Lib-240301
Tecan_Location
Tecan
Tecan_date
Size_bp299302
Peak_Molarity110.0088.00
libSampleQC_DNA_WellDNA_Library_set70/set1/C3.pngDNA_Library_set70/set1/D3.png
RIN9.99.9
libSample_RNA_WellRNA_Library_set70/set1/G8.pngRNA_Library_set70/set1/H8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-03-142024-03-14
Sample_IDBGHcLV41-39BGHcLV41-40
RBPEIF5BEIF5B
Batch_IDBGHcLV41BGHcLV41
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42444245




Sequencing Information
BGHcLV41-39BGHcLV41-40
Sample_IDBGHcLV41-39BGHcLV41-40
Sample NameEIF5B-BGHcLV41-39EIF5B-BGHcLV41-40
Pool IDPool-240314Pool-240314
LocalServer_folderset70set70
total_reads31,259,83338,564,119
total_aligned_reads29,394,61536,291,807
unique_aligned_reads27,861,41434,291,632
percent_uniqueAligned0.891280.88921
correlation_replicates0.9763890.976389
spikein_reads3,4621,322
percent_spikeins0.000110.00003
original_ReadLength151151
QC_StatusSubmittingSubmitting
ID29632964




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database