BRD2

General Information

Gene Name : BRD2
Gene ID : ENSG00000204256.12
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: BRD2)

Not Available

Antibody Validation

View validation images for all BRD2 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	BRD2	ENSG00000204256.12	182.62	159.18	170.90	ENCSR000AEF
HepG2	BRD2	ENSG00000204256.12	133.87	160.23	147.05	ENCSR985KAT
K562	BRD2	ENSG00000204256.12	152.50	169.55	161.03	ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	BRD2-BGKcLV26-K562	ENCSR046XQE	BRD2	K562	Released		ENCSR900GQL	CRISPR	KD% = 73.4,72.1

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
BRD2	ENCAB518SCS	A302-582A	2			Released, ENCODE4		BGKcLV26-41, Submitted, ENCORE2
BRD2	ENCAB151CIF	A302-583A	6			Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
BRD2	K562	CRISPR	ENCSR046XQE	ENCSR900GQL	BRD2-BGKcLV26-41,BRD2-BGKcLV26-42	NT-BGKcLV26-1,NT-BGKcLV26-2	73.4,72.1	Released	ENCODE4
BRD2	HepG2	CRISPR			BGHcLV27-25,BGHcLV27-26	BGHcLV27-1,BGHcLV27-2	NO clear band,	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
BRD2	Product_ID: A302-582A Lot_ID: 2 Source: Bethyl Labs Target Name: BRD2-human
BRD2	Product_ID: A302-583A Lot_ID: 6 Source: Bethyl Labs Target Name: BRD2-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for BRD2 is not avaliable on ENCORE database.

Proteins bound to RNA

BRD2 RNA is bound by 69 proteins.

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eCLIP assay for BRD2 is not avaliable on ENCORE database.

69 proteins are bound to BRD2 RNA:

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Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr6	32942586	32942615	+	intron-Proximal	5.14741	67.75756	K562	EFTUD2
chr6	32948959	32949065	+	3'-UTR	3.85164	66.34170	K562	UPF1
chr6	32944017	32944058	+	3'-UTR,CDS,intron-Proximal	4.88739	64.08131	K562	U2AF2
chr6	32942193	32942296	+	5'-UTR,CDS,intron-Proximal	4.45553	63.12745	HepG2	BUD13
chr6	32939594	32939624	+	5'-UTR,CDS,intron-Distal	6.15835	61.06627	K562	RBM15
chr6	32942646	32942741	+	intron-Distal,intron-Proximal	5.20743	61.00744	K562	EFTUD2
chr6	32942211	32942282	+	5'-UTR,CDS,intron-Proximal	5.51052	59.68108	K562	AQR
chr6	32948966	32948995	+	3'-UTR	3.85383	59.33934	K562	TIA1
chr6	32942622	32942687	+	intron-Distal,intron-Proximal	5.02334	56.63231	HepG2	EFTUD2
chr6	32945960	32946086	+	3'-UTR,CDS	4.02103	53.14049	K562	UCHL5
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 378 entries

eCLIP assay for BRD2 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for BRD2 is not available on ENCORE database.
98 RBPs can affect the levels of BRD2.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for BRD2 is not available on ENCORE database.

98 RBPs can affect the gene expression of BRD2 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
BRD2	ENSG00000204256.8	41716.47880	8447.02270	2.30400	106.82180	103.76650	K562	AQR
BRD2	ENSG00000204256.8	11240.67650	6482.24800	0.79410	44.21850	42.24080	HepG2	UTP3
BRD2	ENSG00000204256.8	14195.71950	5756.54390	1.30210	39.54620	37.10780	HepG2	U2AF1
BRD2	ENSG00000204256.8	11917.64630	5813.48550	1.03550	34.27280	32.24620	K562	HNRNPC
BRD2	ENSG00000204256.8	9356.85640	6118.80170	0.61270	27.66160	26.50650	HepG2	SRFBP1
BRD2	ENSG00000204256.8	16442.35050	8571.29610	0.93970	22.30380	20.78590	K562	PUS1
BRD2	ENSG00000204256.8	7776.38840	4784.24740	0.70070	21.19960	19.63390	K562	FXR1
BRD2	ENSG00000204256.8	9508.26580	6481.64780	0.55270	18.83300	17.49860	HepG2	ETF1
BRD2	ENSG00000204256.8	11902.38060	8306.10400	0.51890	18.60690	17.25520	K562	AGGF1
BRD2	ENSG00000204256.8	13833.92440	8351.50140	0.72800	18.71990	17.14060	K562	RBM15
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 119 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for BRD2 is not available on ENCORE database.

31 RBPs can affect the splicing of BRD2 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	BRD2	ENSG00000204256.8	chr6	+	32942797	32942889	32942238	32942542	32943160	32943298	-0.09700	0.00000	excluded	K562	SF3B4
SE	BRD2	ENSG00000204256.8	chr6	+	32942797	32942889	32942238	32942542	32943160	32943298	-0.19600	0.00000	excluded	HepG2	U2AF1
SE	BRD2	ENSG00000204256.8	chr6	+	32942797	32942889	32942238	32942542	32943160	32943298	-0.10600	0.00006	excluded	K562	AKAP8L
SE	BRD2	ENSG00000204256.8	chr6	+	32942797	32942889	32942238	32942542	32943160	32943298	-0.13400	0.00000	excluded	K562	PUS1
SE	BRD2	ENSG00000204256.8	chr6	+	32942797	32942889	32942238	32942542	32943160	32943298	-0.09700	0.00016	excluded	HepG2	SF3A3
SE	BRD2	ENSG00000204256.8	chr6	+	32942797	32942889	32942238	32942542	32943160	32943298	-0.12300	0.00000	excluded	K562	RBM15
SE	BRD2	ENSG00000204256.8	chr6	+	32942797	32942889	32942238	32942542	32943160	32943298	-0.10400	0.00012	excluded	K562	AGGF1
SE	BRD2	ENSG00000204256.8	chr6	+	32942797	32942889	32942238	32942542	32943160	32943298	0.13000	0.00002	included	K562	HNRNPC
A3SS	BRD2	ENSG00000204256.8	chr6	+	32945036	32945347	32945218	32945347	32944338	32944713	0.05500	0.00000	longer	K562	AQR
A3SS	BRD2	ENSG00000204256.8	chr6	+	32948322	32948543	32948358	32948543	32948058	32948241	0.08700	0.00000	longer	K562	AQR

Showing 1 to 10 of 55 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

BRD2

General Information

Functions

Localization

Domains (AlphaFold: BRD2)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for BRD2 is not avaliable on ENCORE database.

69 proteins are bound to BRD2 RNA:

eCLIP assay for BRD2 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for BRD2 is not available on ENCORE database.

98 RBPs can affect the gene expression of BRD2 :

The Knockdown/knockout RNA-Seq assay for BRD2 is not available on ENCORE database.

31 RBPs can affect the splicing of BRD2 :

The Knockdown/knockout RNA-Seq assay for BRD2 is not avaliable on ENCORE database.