RPL28

General Information

Gene Name       : RPL28
Gene ID             : ENSG00000108107.14
Essential Gene : No

Functions

Localization

Domains (AlphaFold: RPL28)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RPL28ENSG00000108107.14142.171196.34669.25ENCSR000AEF
HepG2RPL28ENSG00000108107.141046.46279.00662.73ENCSR985KAT
K562RPL28ENSG00000108107.141496.401343.571419.99ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RPL28-BGKcLV15-K562ENCSR234FWRRPL28K562ReleasedENCSR404OHQ   CRISPRKD% = 64.3,52.0
knockdown followed by RNA-seq RPL28-BGHcLV18-HepG2ENCSR687ITWRPL28HepG2ReleasedENCSR670KAS   CRISPRKD% = 91.6,93.4






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RPL28ENCAB131AOZLS-C352792NotAvailableBGKcLV15-53, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RPL28K562CRISPRENCSR234FWRENCSR404OHQRPL28-BGKcLV15-53,RPL28-BGKcLV15-54NT-BGKcLV15-1,NT-BGKcLV15-264.3,52.0ReleasedENCODE4
RPL28HepG2CRISPRBGHcLV16-49,BGHcLV16-50BGHcLV16-1,BGHcLV16-20.0,0.0NotSatisfiedENCODE4
RPL28HepG2CRISPRENCSR687ITWENCSR670KASRPL28-BGHcLV18-41,RPL28-BGHcLV18-42NT-BGHcLV18-1,NT-BGHcLV18-291.6,93.4ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPL28Product_ID: LS-C352792
Lot_ID: NotAvailable
Source: LSBio
Target Name: RPL28-human		 RPL28-K562-CRIPSR-LS-C352792.png<br>Caption: Western blot following CRISPR against RPL28 in K562 whole cell lysate using RPL28 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPL28. RPL28 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for RPL28 is not avaliable on ENCORE database.



Proteins bound to RNA
  • RPL28 RNA is bound by 17 proteins.  







eCLIP assay for RPL28 is not avaliable on ENCORE database.





17 proteins are bound to RPL28 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr195590033655900432+3'-UTR,intron-Distal3.4817213.70441K562UPF1
chr195590023455900303+3'-UTR,intron-Distal6.1100115.20889K562UPF1
chr195590017655900234+3'-UTR,intron-Distal5.558148.76530K562UPF1
chr195590145655901491+3'-UTR,intron-Distal3.699436.84689K562UPF1
chr195590056555900627+3'-UTR,intron-Distal4.010243.35877HepG2UPF1
chr195590110055901164+3'-UTR,intron-Distal4.8714112.56575K562UPF1
chr195589957755899613+3'-UTR,CDS,intron-Distal,intron-Proximal4.761264.27476HepG2AQR
chr195590117255901245+3'-UTR,intron-Distal3.828803.17235HepG2HNRNPK
chr195589941555899457+3'-UTR,CDS,intron-Proximal4.693515.68078HepG2AQR
chr195589955055899624+3'-UTR,CDS,intron-Distal,intron-Proximal3.797694.74839HepG2XRN2
chr195589957755899621+3'-UTR,CDS,intron-Distal,intron-Proximal3.234826.02419K562AQR
chr195589981255899845+3'-UTR,intron-Distal4.2587110.66391K562YBX3
chr195589953255899625+3'-UTR,CDS,intron-Distal,intron-Proximal4.056309.84780HepG2NKRF
chr195589811555898174+3'-UTR,CDS,intron-Distal,intron-Proximal3.196674.65130K562PRPF8
chr195590211655902164+3'-UTR,intron-Distal4.510939.20161K562UPF1
chr195590252755902583+3'-UTR,intron-Distal4.8562211.38624K562UPF1
chr195589940955899487+3'-UTR,CDS,intron-Proximal3.966165.73400HepG2PPIG
chr195589984555899908+3'-UTR,intron-Distal3.658878.00668K562YBX3
chr195590244755902527+3'-UTR,intron-Distal4.4755517.66749K562UPF1
chr195590043255900539+3'-UTR,intron-Distal4.2981920.81548K562UPF1
chr195590078155900831+3'-UTR,intron-Distal4.722666.69007K562YBX3
chr195589954155899621+3'-UTR,CDS,intron-Distal,intron-Proximal4.009486.94346HepG2BUD13
chr195590056455900644+3'-UTR,intron-Distal6.3638618.82180K562UPF1
chr195590031855900403+3'-UTR,intron-Distal3.523555.09665HepG2HNRNPK
chr195589966855899743+3'-UTR,CDS,intron-Distal,polyA_site3.681354.95875K562IGF2BP2
chr195590013855900223+3'-UTR,intron-Distal3.792703.47885K562LIN28B
chr195590177155901814+3'-UTR,intron-Distal3.165356.18673K562UPF1
chr195590225155902308+3'-UTR,intron-Distal5.6860111.61633K562UPF1
chr195590120955901322+3'-UTR,intron-Distal3.8011014.48199K562UPF1
chr195589807355898127+3'-UTR,CDS,intron-Proximal4.277073.78162K562HNRNPM
chr195589733055897334+5'-UTR,intron-Proximal3.7302752.97556HepG2LARP4
chr195589771755897731+5'-UTR,CDS,intron-Proximal3.6712348.94761HepG2LARP4
chr195589731655897330+5'-UTR3.7365852.74795HepG2LARP4
chr195589729955897316+5'-UTR3.7288752.52040HepG2LARP4
chr195589730055897334+5'-UTR,intron-Proximal3.087894.25070HepG2DDX3X
chr195589733355897383+5'-UTR,intron-Proximal3.906473.39008HepG2AQR
chr195589763855897711+5'-UTR,intron-Proximal4.052263.56713HepG2SF3B4
chr195589732955897340+5'-UTR,intron-Proximal5.03020400.00000K562PABPC4
chr195589730155897321+5'-UTR3.127387.36020HepG2RPS3
chr195589730255897329+5'-UTR5.82344400.00000K562PABPC4
chr195589734555897397+5'-UTR,intron-Proximal6.181139.75034HepG2PRPF8
chr195589733455897340+5'-UTR,intron-Proximal3.716633.59926K562DDX3X
chr195589729955897330+5'-UTR3.1063737.10199K562DDX3X
chr195589771755897730+5'-UTR,CDS,intron-Proximal5.08253400.00000K562PABPC4
chr195589923655899310+CDS,intron-Proximal3.659485.30514HepG2BUD13
chr195589922155899300+CDS,intron-Proximal4.512228.29703HepG2AQR
chr195589804955898112+CDS,intron-Proximal4.271639.08333HepG2PRPF8
chr195589944155899481+CDS,intron-Proximal6.4730817.53326K562AQR
chr195589941655899441+CDS,intron-Proximal5.1882713.23057K562AQR
chr195589780455897853+CDS,intron-Proximal4.413425.34978HepG2PRPF8
chr195589806155898116+CDS,intron-Proximal4.442346.99120K562AQR
chr195589804455898107+CDS,intron-Proximal3.465133.99908K562BUD13
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RPL28 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RPL28 is not available on ENCORE database.

  • 109 RBPs can affect the levels of RPL28.  








The Knockdown/knockout RNA-Seq assay for RPL28 is not available on ENCORE database.





109 RBPs can affect the gene expression of RPL28 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RPL28ENSG00000108107.87256.5552013715.82400-0.918504.002803.38000HepG2AKAP1
RPL28ENSG00000108107.84796.705003466.704400.468403.289802.26970HepG2BCCIP
RPL28ENSG00000108107.82658.786005197.09710-0.966902.756501.99300HepG2CNOT7
RPL28ENSG00000108107.87352.745505857.759400.327804.568903.65270HepG2DAZAP1
RPL28ENSG00000108107.85830.160804619.468900.335704.124003.12850HepG2DDX19B
RPL28ENSG00000108107.82908.440904273.05480-0.555004.698603.84080HepG2DDX21
RPL28ENSG00000108107.85528.0169013136.31860-1.248706.330705.42240HepG2DDX3X
RPL28ENSG00000108107.83586.413202804.019600.354904.251903.47650HepG2DNAJC2
RPL28ENSG00000108107.88439.0108011985.33480-0.506102.042801.49090HepG2EIF4A3
RPL28ENSG00000108107.87478.018405536.792400.433504.432703.41660HepG2EIF4G2
RPL28ENSG00000108107.85968.875209250.84280-0.6321016.8065015.53030HepG2ETF1
RPL28ENSG00000108107.88186.8550012100.92190-0.563702.129101.51840HepG2FXR1
RPL28ENSG00000108107.87513.4683011221.05460-0.578702.290601.68200HepG2G3BP1
RPL28ENSG00000108107.85959.383103653.509400.7058010.849209.15240HepG2GEMIN5
RPL28ENSG00000108107.89348.0610011369.08200-0.282401.963501.41820HepG2GPKOW
RPL28ENSG00000108107.88788.856005573.432200.657006.087905.07940HepG2HNRNPF
RPL28ENSG00000108107.88657.550305335.589300.6982016.7677015.23370HepG2HNRNPL
RPL28ENSG00000108107.83762.565003155.771900.253601.903701.39190HepG2HNRNPU
RPL28ENSG00000108107.87955.170205488.173400.535504.926803.98680HepG2IGF2BP1
RPL28ENSG00000108107.87862.8526011404.41830-0.536502.062001.46980HepG2IGF2BP2
RPL28ENSG00000108107.84638.934403223.508700.5251021.0080019.20130HepG2ILF2
RPL28ENSG00000108107.85514.266604565.739700.272202.310401.37710HepG2KIF1C
RPL28ENSG00000108107.85687.0361012105.18320-1.089905.093404.21210HepG2MAGOH
RPL28ENSG00000108107.85758.8218012171.59730-1.079705.110304.27710HepG2MATR3
RPL28ENSG00000108107.87298.6620011064.44860-0.600204.580203.31090HepG2MTPAP
RPL28ENSG00000108107.84189.4721012457.21940-1.572108.506107.42600HepG2NCBP2
RPL28ENSG00000108107.84517.536103066.982700.558603.381201.61220HepG2PA2G4
RPL28ENSG00000108107.87030.5914011088.97170-0.657402.915802.17620HepG2PABPC4
RPL28ENSG00000108107.84374.607403123.616800.4858012.2899010.71400HepG2PARN
RPL28ENSG00000108107.87095.5712012836.71460-0.855308.118307.16830HepG2PCBP1
RPL28ENSG00000108107.84372.524303393.606400.365503.898402.43030HepG2PKM
RPL28ENSG00000108107.84650.971902814.688400.724509.174307.87780HepG2PPIL4
RPL28ENSG00000108107.81644.424902628.37090-0.676607.922406.63920HepG2PRPF8
RPL28ENSG00000108107.84876.8663013063.08380-1.4215014.9276013.85360HepG2PTBP1
RPL28ENSG00000108107.86874.093505886.855100.223602.311401.75950HepG2PUM1
RPL28ENSG00000108107.87979.966405882.301700.439907.605806.51800HepG2PUM2
RPL28ENSG00000108107.84106.458105087.79710-0.309102.715902.10410HepG2PUS1
RPL28ENSG00000108107.85418.4390014144.98530-1.3843015.5011014.19480HepG2QKI
RPL28ENSG00000108107.86394.3093012838.48720-1.005606.812405.79530HepG2RBM25
RPL28ENSG00000108107.86838.7788014536.92220-1.087908.671807.81190HepG2NELFE
RPL28ENSG00000108107.88416.9681013541.04630-0.686005.013104.21380HepG2RECQL
RPL28ENSG00000108107.81687.148102599.88770-0.623904.822603.81710HepG2RRP9
RPL28ENSG00000108107.84970.098802810.327500.8224042.0529039.99770HepG2SAFB2
RPL28ENSG00000108107.85066.049204007.212800.338202.268001.41330HepG2SERBP1
RPL28ENSG00000108107.88647.2161010739.26790-0.312603.061202.31920HepG2SF3B1
RPL28ENSG00000108107.86615.3533013598.12070-1.0395010.970909.91300HepG2SF3B4
RPL28ENSG00000108107.83621.899403277.271400.144202.049901.48370HepG2SMN1
RPL28ENSG00000108107.82993.3783011949.24060-1.9971035.6635032.94050HepG2SNRNP70
RPL28ENSG00000108107.87095.906508733.07710-0.299507.105906.40540HepG2SRFBP1
RPL28ENSG00000108107.83754.2410014194.40660-1.9187024.3349023.23610HepG2SRSF1
RPL28ENSG00000108107.84321.825005475.54060-0.341402.530801.92770HepG2SRSF5
RPL28ENSG00000108107.87333.996005475.941700.421405.596504.66350HepG2SRSF7
RPL28ENSG00000108107.86079.6790011548.69170-0.925708.668207.78710HepG2SRSF9
RPL28ENSG00000108107.85204.842807582.45940-0.5428018.0222016.95660HepG2SSB
RPL28ENSG00000108107.85045.7500012761.01810-1.338608.804007.89500HepG2TAF15
RPL28ENSG00000108107.816035.4121010574.087700.600603.635902.81890HepG2TARDBP
RPL28ENSG00000108107.86240.6667011722.95060-0.909605.244304.10650HepG2TIA1
RPL28ENSG00000108107.82057.816903066.25070-0.5754010.556509.68940HepG2TIAL1
RPL28ENSG00000108107.85274.619503093.844700.7696014.3775013.23360HepG2TRIM56
RPL28ENSG00000108107.84345.956205418.06640-0.318102.203701.42990HepG2TUFM
RPL28ENSG00000108107.86818.126409654.34560-0.501803.166402.49720HepG2U2AF1
RPL28ENSG00000108107.82830.384802004.103700.497904.455503.49160HepG2UCHL5
RPL28ENSG00000108107.86512.078208350.81900-0.358809.614108.44770HepG2UPF1
RPL28ENSG00000108107.87106.936708583.46360-0.272304.708203.37830HepG2UTP18
RPL28ENSG00000108107.810318.751308062.385500.355905.924305.10290HepG2UTP3
RPL28ENSG00000108107.86200.000103340.024300.8923014.5167012.87980HepG2XRN2
RPL28ENSG00000108107.87482.396909184.30040-0.295708.922908.08410HepG2ZNF622
RPL28ENSG00000108107.840890.9477022618.963300.8541054.0513051.61970K562AGGF1
RPL28ENSG00000108107.833322.7382024854.942200.422905.758604.21590K562AKAP8
RPL28ENSG00000108107.838805.9086023536.086200.721302.020501.46040K562AKAP8L
RPL28ENSG00000108107.815708.1384018499.23190-0.236002.608701.40490K562APOBEC3C
RPL28ENSG00000108107.823327.3493012186.566400.9366024.2587022.71360K562AQR
RPL28ENSG00000108107.832924.6894024695.076300.414806.421304.95700K562ATP5C1
RPL28ENSG00000108107.833705.1885021984.975000.616403.837502.09500K562CKAP4
RPL28ENSG00000108107.87790.252406180.224300.333903.676102.43290K562CSTF2
RPL28ENSG00000108107.815636.1196011601.490900.4305010.199007.09080K562DDX24
RPL28ENSG00000108107.813166.0788010949.023100.265902.359901.40120K562DDX3X
RPL28ENSG00000108107.814798.2432023314.40590-0.6558027.3920025.54810K562DDX47
RPL28ENSG00000108107.813149.3579011101.009400.244203.467901.96120K562DDX6
RPL28ENSG00000108107.836638.2113025326.328500.532604.966603.74040K562DKC1
RPL28ENSG00000108107.814602.4796012027.193700.279805.448903.72390K562EIF2S1
RPL28ENSG00000108107.85456.556803955.625700.464003.296102.43050K562EIF3G
RPL28ENSG00000108107.814209.7800011439.264100.312803.349002.45000K562EIF4A3
RPL28ENSG00000108107.813519.0809010301.904800.392002.583401.48110K562EIF4B
RPL28ENSG00000108107.815324.368509985.462100.6178012.767809.68940K562EWSR1
RPL28ENSG00000108107.820032.8065013793.955400.538208.294006.73560K562FIP1L1
RPL28ENSG00000108107.810190.0278011896.39410-0.223402.422501.84930K562FXR1
RPL28ENSG00000108107.81758.402005755.94970-1.710808.464607.08530K562G3BP2
RPL28ENSG00000108107.818618.1961014582.981500.352302.528701.80300K562GRSF1
RPL28ENSG00000108107.828519.7172018305.188200.639603.002601.71750K562HNRNPA2B1
RPL28ENSG00000108107.814998.6243011346.302300.402503.675702.58960K562HNRNPM
RPL28ENSG00000108107.830266.3017018700.865900.6945013.1749011.48120K562HSPD1
RPL28ENSG00000108107.814734.889807571.309700.960503.826502.54420K562IGF2BP2
RPL28ENSG00000108107.811274.360807699.344500.550103.118501.83540K562IGF2BP3
RPL28ENSG00000108107.832110.7167024977.565900.362305.149203.95390K562KIF1C
RPL28ENSG00000108107.836503.0381023909.761100.610305.635603.91430K562MAK16
RPL28ENSG00000108107.826919.9458019612.802100.456803.117002.17250K562NAA15
RPL28ENSG00000108107.811923.012907234.326900.720704.365802.63610K562NONO
RPL28ENSG00000108107.826464.8425019744.761500.422506.454805.37790K562NSUN2
RPL28ENSG00000108107.830412.1265016008.452600.925705.868804.26710K562PABPN1
RPL28ENSG00000108107.810050.733507775.960000.370103.061501.63710K562PCBP1
RPL28ENSG00000108107.812288.1457021797.31910-0.826902.931302.10630K562POLR2G
RPL28ENSG00000108107.826079.7876015659.700000.735806.021304.73090K562PPIG
RPL28ENSG00000108107.817859.2350022874.76290-0.357104.490303.45600K562PUF60
RPL28ENSG00000108107.820922.9743024334.97150-0.217902.943201.84020K562PUM1
RPL28ENSG00000108107.848100.2696023341.129201.043103.251102.67020K562PUS1
RPL28ENSG00000108107.839997.3054022742.588200.814409.398908.32890K562RBM15
RPL28ENSG00000108107.88096.871105837.244700.472004.368602.12120K562RECQL
RPL28ENSG00000108107.825914.4102019480.418000.411604.970803.90550K562SF3A3
RPL28ENSG00000108107.88819.876105979.893400.560503.964002.93410K562SF3B4
RPL28ENSG00000108107.831974.7512017842.705900.841502.575001.33840K562SFPQ
RPL28ENSG00000108107.814835.847209115.946600.702506.186204.48600K562SRFBP1
RPL28ENSG00000108107.811336.936007130.017100.669005.891103.39460K562SRP68
RPL28ENSG00000108107.816186.8040019957.87210-0.302102.607401.69400K562SRPK2
RPL28ENSG00000108107.825291.8189016519.041400.614402.858601.78380K562SRSF4
RPL28ENSG00000108107.811838.197207155.752000.7262013.3640010.89940K562SRSF5
RPL28ENSG00000108107.819292.2015015842.213700.284104.683103.14040K562SRSF7
RPL28ENSG00000108107.811818.110806906.395200.774904.487803.16750K562SUPV3L1
RPL28ENSG00000108107.812788.587807665.980600.738206.892105.72620K562TARDBP
RPL28ENSG00000108107.89543.080206298.063700.599405.051102.82540K562TFIP11
RPL28ENSG00000108107.811441.865207394.971600.629606.426305.10140K562TIA1
RPL28ENSG00000108107.811667.465007259.078500.684504.123302.74630K562TRA2A
RPL28ENSG00000108107.827694.5638024439.174000.180302.831101.54630K562TROVE2
RPL28ENSG00000108107.810796.037407311.580500.562104.391003.07840K562UCHL5
RPL28ENSG00000108107.819958.0906024949.59770-0.322002.580901.42560K562UTP3
RPL28ENSG00000108107.811651.395707448.406400.645405.626103.68140K562XRN2
RPL28ENSG00000108107.816645.4135013726.688900.278002.808601.93770K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RPL28 is not available on ENCORE database.





7 RBPs can affect the splicing of RPL28 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SERPL28ENSG00000108107.8chr19+5589961055899852558992755589941655902921559031070.054000.002260.09183includedK562EFTUD2
SERPL28ENSG00000108107.8chr19+558996105589985255899297558994165590292155903107-0.247000.000000.00000excludedHepG2SRSF1
SERPL28ENSG00000108107.8chr19+558996105589985255899297558994165590292155903107-0.092000.000000.00000excludedHepG2PCBP1
SERPL28ENSG00000108107.8chr19+558996105589985255899297558994165590292155903107-0.219000.000000.00000excludedHepG2TAF15
SERPL28ENSG00000108107.8chr19+558996105589985255899297558994165590292155903107-0.118000.000000.00000excludedHepG2SRSF9
A5SSRPL28ENSG00000108107.8chr19+5589793755898050558979375589804255899297558994160.089000.001860.03166longerK562AQR
A5SSRPL28ENSG00000108107.8chr19+558979375589805055897937558980425589929755899416-0.237000.001400.05030shorterHepG2U2AF2
RIRPL28ENSG00000108107.8chr19+5589793755899416558979375589806155899297558994160.053000.000000.00000includedK562AQR
RIRPL28ENSG00000108107.8chr19+5589732855897806558973285589754655897717558978060.105000.012470.06538includedK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for RPL28 is not avaliable on ENCORE database.