SMYD3

General Information

Gene Name : SMYD3
Gene ID : ENSG00000185420.18
Essential Gene : No

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: SMYD3)

Not Available

Antibody Validation

Thermo Fisher: PA5-31919

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	SMYD3	ENSG00000185420.18	18.03	12.48	15.26	ENCSR000AEF
HepG2	SMYD3	ENSG00000185420.18	21.86	19.19	20.52	ENCSR985KAT
K562	SMYD3	ENSG00000185420.18	101.42	103.51	102.47	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	SMYD3-BGKcLV19-K562	ENCSR775USN	SMYD3	K562	Released		ENCSR978MMZ	CRISPR	KD% = 91.4,90.6
knockdown followed by RNA-seq	SMYD3-BGHcLV20-HepG2	ENCSR989GNV	SMYD3	HepG2	Released		ENCSR306EWE	CRISPR	KD% = 99.0,99.0

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
SMYD3	ENCAB101UYP	PA5-31919	UA2701668D			Released, ENCODE4	BGHcLV20-35, Released, ENCODE4	BGKcLV19-49, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
SMYD3	K562	CRISPR			BGKcLV19-49,BGKcLV19-50	BGKcLV19-1,BGKcLV19-2	88.7,89.2	NotSatisfied	ENCODE4
SMYD3	K562	CRISPR	ENCSR775USN	ENCSR978MMZ	SMYD3-BGKcLV19-51,SMYD3-BGKcLV19-52	NT-BGKcLV19-1,NT-BGKcLV19-2	91.4,90.6	Released	ENCODE4
SMYD3	HepG2	CRISPR	ENCSR989GNV	ENCSR306EWE	SMYD3-BGHcLV20-35,SMYD3-BGHcLV20-36	NT-BGHcLV20-1,NT-BGHcLV20-2	99.0,99.0	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
SMYD3	Product_ID: PA5-31919 Lot_ID: UA2701668D Source: Thermo Fisher Target Name: SMYD3-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for SMYD3 is not avaliable on ENCORE database.

Proteins bound to RNA

SMYD3 RNA is bound by 61 proteins.

CanvasJS.com

eCLIP assay for SMYD3 is not avaliable on ENCORE database.

61 proteins are bound to SMYD3 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr1	246591917	246592128	-	intron-Distal	7.67874	96.94041	K562	DROSHA
chr1	245961598	245961708	-	intron-Distal	6.73754	57.63913	K562	EIF4G2
chr1	246488312	246488382	-	intron-Distal	8.07894	55.68701	HepG2	PCBP2
chr1	246647160	246647270	-	intron-Distal,intron-Proximal	7.43663	42.28135	K562	EXOSC5
chr1	246592069	246592140	-	intron-Distal	6.47629	39.54783	HepG2	RBFOX2
chr1	246488196	246488312	-	intron-Distal	6.04620	37.92990	HepG2	PCBP2
chr1	246309039	246309161	-	intron-Distal	7.27471	33.79917	HepG2	QKI
chr1	246283145	246283222	-	intron-Distal	4.86833	31.89509	K562	CSTF2T
chr1	246309056	246309159	-	intron-Distal	5.63723	31.45872	K562	QKI
chr1	246592024	246592069	-	intron-Distal	6.98923	30.06238	HepG2	RBFOX2
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 579 entries

eCLIP assay for SMYD3 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for SMYD3 is not available on ENCORE database.
79 RBPs can affect the levels of SMYD3.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for SMYD3 is not available on ENCORE database.

79 RBPs can affect the gene expression of SMYD3 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SMYD3	ENSG00000185420.14	208.67420	645.89560	-1.63000	30.52990	28.80290	HepG2	QKI
SMYD3	ENSG00000185420.14	818.02210	1619.24770	-0.98510	26.64520	24.89060	K562	FXR1
SMYD3	ENSG00000185420.14	2470.88480	1390.09590	0.82970	20.30350	18.61860	K562	EIF2S2
SMYD3	ENSG00000185420.14	1048.17430	1979.86710	-0.91750	19.71150	17.82160	K562	SRSF1
SMYD3	ENSG00000185420.14	241.60440	527.26310	-1.12590	17.89150	16.71160	HepG2	SRSF9
SMYD3	ENSG00000185420.14	211.92540	606.65390	-1.51730	18.01320	16.51860	HepG2	MATR3
SMYD3	ENSG00000185420.14	490.70760	1260.64000	-1.36120	18.27510	16.21040	K562	PCBP3
SMYD3	ENSG00000185420.14	1122.37040	2074.47500	-0.88620	18.59140	16.13730	K562	MATR3
SMYD3	ENSG00000185420.14	376.74830	967.00530	-1.35990	17.86800	15.92990	K562	POLR2G
SMYD3	ENSG00000185420.14	301.98640	620.91030	-1.03990	15.63070	14.40140	HepG2	SF3B4
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 89 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for SMYD3 is not available on ENCORE database.

13 RBPs can affect the splicing of SMYD3 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942			246027100	246027188	246091232	246091335	-0.30900	0.00000	0.00000	excluded	K562	SF3B4
SE	SMYD3	ENSG00000185420.14	chr1	-	245927342	245927451			245912864	245912966	246021797	246021972	-0.10700	0.00000	0.00018	excluded	K562	EFTUD2
SE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942			246027100	246027188	246091232	246091335	-0.25700	0.00000	0.00009	excluded	K562	SF3B1
MXE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942	246091232	246091385	246027100	246027188	246093171	246093239	0.17500	0.00000	0.00001	1included	K562	AQR
MXE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942	246091232	246091358	246027100	246027188	246093171	246093239	0.17200	0.00000	0.00002	1included	K562	AQR
SE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942			246027100	246027188	246091232	246091358	-0.13100	0.00001	0.00021	excluded	K562	AQR
SE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942			246027100	246027188	246091232	246091335	-0.06700	0.00001	0.00046	excluded	K562	PUS1
SE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942			246027100	246027188	246091232	246091335	-0.12500	0.00002	0.00098	excluded	K562	U2AF1
SE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942			246027100	246027188	246091232	246091335	-0.07000	0.00003	0.00139	excluded	K562	RBM15
MXE	SMYD3	ENSG00000185420.14	chr1	-	246078831	246078942	246091232	246091314	246027100	246027188	246151695	246151864	0.07900	0.00003	0.00134	1included	K562	PUS1

Showing 1 to 10 of 25 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

SMYD3

General Information

Functions

Localization

Domains (AlphaFold: SMYD3)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for SMYD3 is not avaliable on ENCORE database.

61 proteins are bound to SMYD3 RNA:

eCLIP assay for SMYD3 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for SMYD3 is not available on ENCORE database.

79 RBPs can affect the gene expression of SMYD3 :

The Knockdown/knockout RNA-Seq assay for SMYD3 is not available on ENCORE database.

13 RBPs can affect the splicing of SMYD3 :

The Knockdown/knockout RNA-Seq assay for SMYD3 is not avaliable on ENCORE database.