RNASEH2A

General Information

Gene Name       : RNASEH2A
Gene ID             : ENSG00000104889.6
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RNASEH2A)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RNASEH2AENSG00000104889.66.5820.8613.72ENCSR000AEF
HepG2RNASEH2AENSG00000104889.652.2433.0142.62ENCSR985KAT
K562RNASEH2AENSG00000104889.653.8731.5342.70ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RNASEH2A-BGKcLV30-K562ENCSR002JOWRNASEH2AK562ReleasedENCSR347NKX   CRISPRKD% = 68.3,66.9
knockdown followed by RNA-seq RNASEH2A-BGHcLV32-HepG2ENCSR703FPCRNASEH2AHepG2ReleasedENCSR232ERY   CRISPRKD% = 93.8,94.7






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RNASEH2AENCAB029VMCA304-149A1Released, ENCODE4BGHcLV32-35, Released, ENCODE4BGKcLV30-37, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RNASEH2AK562CRISPRENCSR002JOWENCSR347NKXRNASEH2A-BGKcLV30-37,RNASEH2A-BGKcLV30-38NT-BGKcLV30-1,NT-BGKcLV30-268.3,66.9ReleasedENCODE4
RNASEH2AHepG2CRISPRENCSR703FPCENCSR232ERYRNASEH2A-BGHcLV32-35,RNASEH2A-BGHcLV32-36NT-BGHcLV32-1,NT-BGHcLV32-293.8,94.7ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RNASEH2AProduct_ID: A304-149A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RNASEH2A-human		 RNASEH2A-HEPG2-CRISPR-A304-149A.png<br>Caption: Western blot following CRISPR against RNASEH2A in HepG2 whole cell lysate using RNASEH2A specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RNASEH2A. RNASEH2A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A304-149A_1_RNASEH2A.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RNASEH2A specific antibody, A304-149A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RNASEH2A-specific antibody, A304-149A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RNASEH2A-K562-CRISPR-A304-149A.png<br>Caption: Western blot following CRISPR against RNASEH2A in K562 whole cell lysate using RNASEH2A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RNASEH2A. RNASEH2A protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for RNASEH2A is not avaliable on ENCORE database.



Proteins bound to RNA
  • RNASEH2A RNA is bound by 6 proteins.  







eCLIP assay for RNASEH2A is not avaliable on ENCORE database.





6 proteins are bound to RNASEH2A RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr191292422912924282+3'-UTR,CDS4.214223.72779HepG2LARP4
chr191292423612924293+3'-UTR,CDS4.9104110.44987K562PABPC4
chr191291740512917448+5'-UTR4.679735.07098K562DDX3X
chr191291744812917512+5'-UTR,CDS4.6070616.20885K562DDX3X
chr191291757712917614+CDS4.102917.63469K562DDX3X
chr191291823212918272+CDS,intron-Proximal3.525868.63185K562YBX3
chr191292414112924176+CDS,intron-Proximal4.267293.83925K562YBX3
chr191291813712918180+CDS,intron-Proximal3.903994.75655HepG2PRPF8
chr191291751012917616+CDS,intron-Proximal4.581506.15796HepG2DDX3X
chr191291793212918050+CDS,intron-Proximal3.993245.00154HepG2AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RNASEH2A is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RNASEH2A is not available on ENCORE database.

  • 105 RBPs can affect the levels of RNASEH2A.  








The Knockdown/knockout RNA-Seq assay for RNASEH2A is not available on ENCORE database.





105 RBPs can affect the gene expression of RNASEH2A :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RNASEH2AENSG00000104889.4316.76870458.42760-0.533304.766404.00840HepG2ACO1
RNASEH2AENSG00000104889.4331.55040460.50910-0.474003.763502.88190HepG2AKAP8L
RNASEH2AENSG00000104889.4127.66030342.47960-1.4237012.6967011.12040HepG2ASCC1
RNASEH2AENSG00000104889.4286.30640654.71650-1.1933022.0735020.47780HepG2AUH
RNASEH2AENSG00000104889.4160.06490343.55730-1.101908.509107.04570HepG2BCLAF1
RNASEH2AENSG00000104889.4107.97060336.86630-1.6415016.8671015.57110HepG2BOP1
RNASEH2AENSG00000104889.4123.52970298.78740-1.274305.402204.37020HepG2CALR
RNASEH2AENSG00000104889.4219.49410325.38120-0.567902.848302.06420HepG2CIRBP
RNASEH2AENSG00000104889.4152.54960458.24100-1.5868023.9900022.46710HepG2CKAP4
RNASEH2AENSG00000104889.4171.21780309.25390-0.853005.476004.53900HepG2CPSF6
RNASEH2AENSG00000104889.4405.88250629.34080-0.632807.068606.13050HepG2CELF1
RNASEH2AENSG00000104889.4208.87460326.57520-0.644803.897603.10640HepG2DDX21
RNASEH2AENSG00000104889.4167.63810319.65010-0.931107.149305.69100HepG2DDX24
RNASEH2AENSG00000104889.4140.48670396.49280-1.4969014.8158013.48190HepG2DDX3X
RNASEH2AENSG00000104889.4202.83620388.80970-0.938706.703505.56130HepG2DDX55
RNASEH2AENSG00000104889.4134.01010351.18310-1.389908.621806.95250HepG2DDX59
RNASEH2AENSG00000104889.4198.54010340.62670-0.778804.337703.57900HepG2DDX6
RNASEH2AENSG00000104889.4325.21200438.56850-0.431402.960902.26270HepG2DKC1
RNASEH2AENSG00000104889.4177.05830277.79820-0.649803.460202.21600HepG2EFTUD2
RNASEH2AENSG00000104889.4142.37220300.78800-1.079107.113805.37030HepG2AGO1
RNASEH2AENSG00000104889.4101.79010327.42180-1.6856015.4083014.07950HepG2EIF2S1
RNASEH2AENSG00000104889.4122.34720270.53150-1.144807.643006.20720HepG2EIF3D
RNASEH2AENSG00000104889.4222.21480352.31560-0.664902.998902.28480HepG2EIF4B
RNASEH2AENSG00000104889.4387.19800667.60140-0.7859010.925609.47870HepG2EIF4G1
RNASEH2AENSG00000104889.4332.98350639.51870-0.9415010.305208.78220HepG2EIF4G2
RNASEH2AENSG00000104889.4256.96040751.72430-1.5487035.8012034.08100HepG2ETF1
RNASEH2AENSG00000104889.4313.49900447.88850-0.514703.542102.59650HepG2EWSR1
RNASEH2AENSG00000104889.4267.41950447.50550-0.742803.920403.01650HepG2FIP1L1
RNASEH2AENSG00000104889.4118.58600309.80010-1.385405.806504.33620HepG2FTO
RNASEH2AENSG00000104889.4543.36370365.111100.573503.716402.92400HepG2FXR1
RNASEH2AENSG00000104889.4247.32410338.67030-0.453502.020901.44890HepG2G3BP1
RNASEH2AENSG00000104889.4170.20990240.86280-0.500902.124001.34380HepG2GEMIN5
RNASEH2AENSG00000104889.4215.07420350.54230-0.704803.929903.17800HepG2GPKOW
RNASEH2AENSG00000104889.4147.31520326.31790-1.147402.699901.35450HepG2HLTF
RNASEH2AENSG00000104889.4179.40910292.37830-0.704603.804403.06430HepG2HNRNPA1
RNASEH2AENSG00000104889.4407.23960621.90070-0.610806.654105.31270HepG2HNRNPA2B1
RNASEH2AENSG00000104889.4483.26810616.41210-0.351102.931802.23990HepG2HNRNPL
RNASEH2AENSG00000104889.498.15420334.53080-1.7690017.1766015.79710HepG2HNRNPLL
RNASEH2AENSG00000104889.4258.54410349.60580-0.435302.426101.74630HepG2IGF2BP1
RNASEH2AENSG00000104889.4101.19950344.24250-1.7662017.7885016.25180HepG2IGF2BP2
RNASEH2AENSG00000104889.4212.32020132.484500.680302.871501.65150HepG2KHDRBS1
RNASEH2AENSG00000104889.4350.21390590.57720-0.753904.941103.82020HepG2LARP4
RNASEH2AENSG00000104889.4266.40520362.30250-0.443602.034501.33600HepG2MSI2
RNASEH2AENSG00000104889.4190.87080357.13280-0.903904.573703.14730HepG2NONO
RNASEH2AENSG00000104889.4196.77810296.64090-0.592102.778202.02790HepG2NSUN2
RNASEH2AENSG00000104889.468.06240127.06800-0.900702.741301.85840HepG2PABPC1
RNASEH2AENSG00000104889.4132.18810334.75470-1.3405011.5266010.07930HepG2PABPC4
RNASEH2AENSG00000104889.4224.47970336.61750-0.584504.064403.35530HepG2PCBP1
RNASEH2AENSG00000104889.4103.62690321.51860-1.633504.792203.45190HepG2PCBP2
RNASEH2AENSG00000104889.4159.41270268.47830-0.752005.166504.14390HepG2PRPF4
RNASEH2AENSG00000104889.471.30290299.82430-2.0721020.6740018.77230HepG2PRPF8
RNASEH2AENSG00000104889.4319.33750640.53490-1.004208.940307.95140HepG2PUF60
RNASEH2AENSG00000104889.4408.83360679.95770-0.7339011.4146010.38150HepG2PUM1
RNASEH2AENSG00000104889.4412.03980679.46000-0.721608.030406.91410HepG2PUM2
RNASEH2AENSG00000104889.4362.99630466.92550-0.363202.584201.98330HepG2PUS1
RNASEH2AENSG00000104889.4257.86780370.94700-0.524603.163102.50230HepG2QKI
RNASEH2AENSG00000104889.4161.27050310.09850-0.943202.502201.83040HepG2RAVER1
RNASEH2AENSG00000104889.4379.58380510.91240-0.428702.995302.10500HepG2RBM15
RNASEH2AENSG00000104889.4336.88830628.29780-0.8992011.5173010.61390HepG2RBM22
RNASEH2AENSG00000104889.4179.09820336.66710-0.910607.051606.01490HepG2RBM25
RNASEH2AENSG00000104889.4481.51880381.196000.337002.044101.56400HepG2NELFE
RNASEH2AENSG00000104889.4515.22410355.048900.537104.674603.90230HepG2RECQL
RNASEH2AENSG00000104889.4122.19600382.47730-1.6462018.8947017.42620HepG2RPLP0
RNASEH2AENSG00000104889.4118.54140304.92980-1.363103.664102.57860HepG2RPS10
RNASEH2AENSG00000104889.497.91530263.27510-1.4270012.4324011.24320HepG2RPS19
RNASEH2AENSG00000104889.4233.59130399.37200-0.773705.696504.57330HepG2RPS2
RNASEH2AENSG00000104889.450.83080296.57740-2.5446025.9873024.06220HepG2RRP9
RNASEH2AENSG00000104889.4378.32740287.703200.395002.517701.89830HepG2SAFB2
RNASEH2AENSG00000104889.4130.56820264.18060-1.016706.552905.11940HepG2SERBP1
RNASEH2AENSG00000104889.4191.39290396.44970-1.050606.837605.74460HepG2SF3A3
RNASEH2AENSG00000104889.4138.82170331.10720-1.2541010.678209.37640HepG2SF3B1
RNASEH2AENSG00000104889.4238.77260356.60010-0.578703.925403.24170HepG2SF3B4
RNASEH2AENSG00000104889.4408.46690614.17640-0.588406.846005.82210HepG2SLBP
RNASEH2AENSG00000104889.4153.83740368.41920-1.259908.154206.81300HepG2SNRNP70
RNASEH2AENSG00000104889.4157.75830709.63100-2.1694058.5084056.97390HepG2SRFBP1
RNASEH2AENSG00000104889.4199.31240356.30730-0.838104.602903.28070HepG2SRSF3
RNASEH2AENSG00000104889.4384.74360632.43680-0.717008.995607.95310HepG2SRSF5
RNASEH2AENSG00000104889.4203.23330348.81350-0.779306.808405.79620HepG2SRSF7
RNASEH2AENSG00000104889.4133.13670302.82890-1.1856012.0646011.05650HepG2SRSF9
RNASEH2AENSG00000104889.4381.00180616.11350-0.693408.865008.04790HepG2SSB
RNASEH2AENSG00000104889.4599.69410719.25330-0.262302.002001.45660HepG2SUCLG1
RNASEH2AENSG00000104889.482.31200387.02190-2.2332026.0372024.35110HepG2SUPV3L1
RNASEH2AENSG00000104889.4113.79960326.12010-1.5189014.0204012.59230HepG2TARDBP
RNASEH2AENSG00000104889.484.62690259.38760-1.615908.978307.39190HepG2TFIP11
RNASEH2AENSG00000104889.4105.65800313.94480-1.5711017.1687016.11640HepG2TIAL1
RNASEH2AENSG00000104889.4476.29790625.77810-0.393802.620601.77170HepG2TUFM
RNASEH2AENSG00000104889.4139.80540382.88070-1.4535013.7600012.32200HepG2U2AF1
RNASEH2AENSG00000104889.481.95330424.34900-2.372403.310402.22770HepG2U2AF2
RNASEH2AENSG00000104889.4360.70040662.37790-0.876906.760405.42290HepG2UBE2L3
RNASEH2AENSG00000104889.4123.45240228.60380-0.888905.262304.20950HepG2UCHL5
RNASEH2AENSG00000104889.4122.02340351.59170-1.5267020.2274018.68090HepG2UPF1
RNASEH2AENSG00000104889.4217.74850361.37630-0.730806.095604.59850HepG2UTP18
RNASEH2AENSG00000104889.4208.39220339.44570-0.703905.344204.55710HepG2UTP3
RNASEH2AENSG00000104889.479.96830386.68150-2.2736041.1428039.67770HepG2ZNF622
RNASEH2AENSG00000104889.41521.610301070.952600.506608.888807.94710K562AGGF1
RNASEH2AENSG00000104889.41017.477801525.33350-0.584106.261105.43810K562AQR
RNASEH2AENSG00000104889.41454.197201169.279200.314503.460302.42870K562ATP5C1
RNASEH2AENSG00000104889.4504.12840898.87680-0.834306.005504.61800K562EFTUD2
RNASEH2AENSG00000104889.4462.57210962.12500-1.056508.267906.32420K562EIF3D
RNASEH2AENSG00000104889.41397.257301733.27690-0.310903.530502.47060K562EIF4G2
RNASEH2AENSG00000104889.4862.72290636.080400.439603.375402.43060K562FIP1L1
RNASEH2AENSG00000104889.4159.35410474.11730-1.573008.830707.42000K562G3BP2
RNASEH2AENSG00000104889.4658.63560901.21500-0.452402.709001.31160K562GEMIN5
RNASEH2AENSG00000104889.4870.46430523.170000.734406.690005.14580K562HNRNPM
RNASEH2AENSG00000104889.41329.70660862.411200.624606.755805.52160K562HSPD1
RNASEH2AENSG00000104889.4262.90310442.97710-0.752702.453801.58000K562KRR1
RNASEH2AENSG00000104889.4871.116001103.47520-0.341103.897002.95160K562MTPAP
RNASEH2AENSG00000104889.4421.77220694.33710-0.719204.003402.82140K562PCBP2
RNASEH2AENSG00000104889.41178.98600722.055100.707308.294206.69530K562PPIG
RNASEH2AENSG00000104889.4496.77670874.71820-0.816206.229905.10410K562PRPF8
RNASEH2AENSG00000104889.4993.64780758.321200.389803.269402.55340K562PTBP1
RNASEH2AENSG00000104889.41542.909601891.09780-0.293602.103901.39180K562PUF60
RNASEH2AENSG00000104889.41375.861401105.087200.316102.395101.88200K562PUS1
RNASEH2AENSG00000104889.4665.76400890.78150-0.420102.556001.77160K562RPS19
RNASEH2AENSG00000104889.4494.69980748.31940-0.597103.546001.75950K562SERBP1
RNASEH2AENSG00000104889.4931.630701209.78930-0.376904.370903.36350K562SF3A3
RNASEH2AENSG00000104889.4330.54630493.36550-0.577803.366802.29290K562SF3B1
RNASEH2AENSG00000104889.4949.180101239.45020-0.384905.440904.18300K562SRPK2
RNASEH2AENSG00000104889.4707.79020895.75620-0.339802.876601.32460K562SRSF9
RNASEH2AENSG00000104889.4565.97910846.52750-0.580804.283402.99610K562SUPV3L1
RNASEH2AENSG00000104889.4961.06610752.310700.353202.316601.66630K562TARDBP
RNASEH2AENSG00000104889.4625.14430820.79900-0.392802.500001.61510K562TIAL1
RNASEH2AENSG00000104889.4242.51530459.05020-0.920604.780403.26640K562U2AF1
RNASEH2AENSG00000104889.4178.34860474.27020-1.411009.155707.68750K562U2AF2
RNASEH2AENSG00000104889.4646.76110896.13970-0.470502.579601.60070K562UCHL5
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RNASEH2A is not available on ENCORE database.






No RBPs affect the alternative splcing events of RNASEH2A on ENCORE database.





The Knockdown/knockout RNA-Seq assay for RNASEH2A is not avaliable on ENCORE database.