CNOT2

General Information

Gene Name : CNOT2
Gene ID : ENSG00000111596.12
Essential Gene : No

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: CNOT2)

Not Available

Antibody Validation

Not Avaiable

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	CNOT2	ENSG00000111596.12	65.84	60.87	63.36	ENCSR000AEF
HepG2	CNOT2	ENSG00000111596.12	52.02	48.37	50.20	ENCSR985KAT
K562	CNOT2	ENSG00000111596.12	66.66	66.79	66.72	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Controls	Method	Note
knockdown followed by RNA-seq	CNOT2-BGKcLV23-K562	ENCSR663BBL	CNOT2	K562	Released	ENCSR877JEM	CRISPR	KD% = 71.6,75.8
knockdown followed by RNA-seq	CNOT2-BGHcLV24-HepG2	ENCSR918MUU	CNOT2	HepG2	Released	ENCSR671FDZ	CRISPR	KD% = 73.0,73.0
knockdown followed by RNA-seq	CNOT2-BG293LV06-WT293		CNOT2	293T	Released		shRNA	KD% = ,

eCLIP

No eCLIP experiment available

Antibody Validation

No Antibody Primary Validation image or Secondary image available

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
CNOT2	K562	CRISPR			BGKcLV21-9,BGKcLV21-10	BGKcLV21-1,BGKcLV21-2	0.0,0.0	NotSatisfied	ENCODE4
CNOT2	K562	CRISPR	ENCSR663BBL	ENCSR877JEM	CNOT2-BGKcLV23-29,CNOT2-BGKcLV23-30	NT-BGKcLV23-1,NT-BGKcLV23-2	71.6,75.8	Released	ENCODE4
CNOT2	HepG2	CRISPR			BGHcLV22-11,BGHcLV22-12	BGHcLV22-1,BGHcLV22-2	0.0,0.0	NotSatisfied	ENCODE4
CNOT2	HepG2	CRISPR	ENCSR918MUU	ENCSR671FDZ	CNOT2-BGHcLV24-21,CNOT2-BGHcLV24-22	NT-BGHcLV24-1,NT-BGHcLV24-2	73.0,73.0	Released	ENCODE4
CNOT2	293T	shRNA			CNOT2-BG293LV02-3,CNOT2-BG293LV02-4	NT-BG293LV02-1,NT-BG293LV02-2	,	Revoked	ENCORE2
CNOT2	293T	shRNA			CNOT2-BG293LV06-21,CNOT2-BG293LV06-22	NT-BG293LV06-1,NT-BG293LV06-2	,	Released	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for CNOT2 is not avaliable on ENCORE database.

Proteins bound to RNA

CNOT2 RNA is bound by 36 proteins.

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eCLIP assay for CNOT2 is not avaliable on ENCORE database.

36 proteins are bound to CNOT2 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr12	70733451	70733583	+	intron-Distal	4.39129	19.26018	K562	RBFOX2
chr12	70637489	70637570	+	5'-UTR,intron-Distal	4.10576	16.42308	K562	CSTF2T
chr12	70683526	70683643	+	intron-Distal	4.09514	14.41060	K562	QKI
chr12	70682741	70682813	+	intron-Distal	5.15799	14.40752	K562	PTBP1
chr12	70682813	70682908	+	intron-Distal	5.33196	14.40752	K562	PTBP1
chr12	70682786	70682848	+	intron-Distal	4.24837	13.00530	HepG2	PTBP1
chr12	70659123	70659199	+	intron-Distal	4.87449	12.92095	HepG2	PTBP1
chr12	70702072	70702192	+	intron-Distal	5.60402	11.99995	K562	FMR1
chr12	70748387	70748455	+	3'-UTR	4.64810	11.84897	K562	PUM2
chr12	70659126	70659231	+	intron-Distal	4.88172	11.73060	K562	PTBP1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 156 entries

eCLIP assay for CNOT2 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for CNOT2 is not available on ENCORE database.
41 RBPs can affect the levels of CNOT2.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for CNOT2 is not available on ENCORE database.

41 RBPs can affect the gene expression of CNOT2 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
CNOT2	ENSG00000111596.7	836.61420	1649.21070	-0.97910	20.41780	18.50910	K562	SRSF1
CNOT2	ENSG00000111596.7	3562.48490	2191.22560	0.70100	10.81160	9.78590	K562	PUS1
CNOT2	ENSG00000111596.7	734.60890	1095.54310	-0.57660	9.59570	8.52090	HepG2	HNRNPU
CNOT2	ENSG00000111596.7	1023.31030	1537.13540	-0.58700	9.64560	7.99280	K562	HNRNPU
CNOT2	ENSG00000111596.7	910.88150	1436.36530	-0.65710	6.64320	5.71600	HepG2	DDX3X
CNOT2	ENSG00000111596.7	1521.57170	1894.01150	-0.31590	5.70560	4.68910	K562	NSUN2
CNOT2	ENSG00000111596.7	880.45020	1137.76160	-0.36990	5.20530	4.37030	HepG2	SMN1
CNOT2	ENSG00000111596.7	1026.17810	1430.00590	-0.47870	4.79080	4.03230	HepG2	PUF60
CNOT2	ENSG00000111596.7	2710.13290	2134.96790	0.34400	4.55580	3.81420	K562	RBM15
CNOT2	ENSG00000111596.7	1276.19220	1682.30300	-0.39860	4.35950	3.54340	K562	PTBP1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 44 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for CNOT2 is not available on ENCORE database.

28 RBPs can affect the splicing of CNOT2 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	CNOT2	ENSG00000111596.7	chr12	+	70688016	70688074	70671911	70672054	70704674	70704797	0.16400	0.00001	included	K562	SRSF1
SE	CNOT2	ENSG00000111596.7	chr12	+	70726546	70726626	70724066	70724249	70729217	70729246	-0.10500	0.00023	excluded	K562	AKAP8L
SE	CNOT2	ENSG00000111596.7	chr12	+	70723202	70723350	70713077	70713144	70729217	70729244	-0.60800	0.00000	excluded	K562	PUS1
SE	CNOT2	ENSG00000111596.7	chr12	+	70726546	70726626	70724066	70724249	70729217	70729246	-0.10000	0.00009	excluded	K562	UTP18
SE	CNOT2	ENSG00000111596.7	chr12	+	70723202	70723350	70713077	70713144	70729217	70729244	-0.36200	0.00000	excluded	K562	AGGF1
SE	CNOT2	ENSG00000111596.7	chr12	+	70679179	70679366	70671911	70672054	70704674	70704797	0.27100	0.00000	included	HepG2	HNRNPC
SE	CNOT2	ENSG00000111596.7	chr12	+	70679179	70679366	70671911	70672054	70704674	70704797	0.57900	0.00000	included	K562	HNRNPC
A3SS	CNOT2	ENSG00000111596.7	chr12	+	70732423	70732602	70732445	70732602	70732222	70732343	0.05800	0.00005	longer	K562	AQR
A3SS	CNOT2	ENSG00000111596.7	chr12	+	70735837	70735948	70735886	70735948	70732423	70732602	0.05800	0.00000	longer	K562	AQR
A3SS	CNOT2	ENSG00000111596.7	chr12	+	70704658	70704797	70704674	70704797	70671911	70672054	0.11400	0.00000	longer	K562	AQR

Showing 1 to 10 of 80 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

CNOT2

General Information

Functions

Localization

Domains (AlphaFold: CNOT2)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for CNOT2 is not avaliable on ENCORE database.

36 proteins are bound to CNOT2 RNA:

eCLIP assay for CNOT2 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for CNOT2 is not available on ENCORE database.

41 RBPs can affect the gene expression of CNOT2 :

The Knockdown/knockout RNA-Seq assay for CNOT2 is not available on ENCORE database.

28 RBPs can affect the splicing of CNOT2 :

The Knockdown/knockout RNA-Seq assay for CNOT2 is not avaliable on ENCORE database.