CD3EAP

General Information

Gene Name       : CD3EAP
Gene ID             : ENSG00000117877.10
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: CD3EAP)


Antibody Validation

 View validation images for all CD3EAP antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CD3EAPENSG00000117877.108.4115.0511.73ENCSR000AEF
HepG2CD3EAPENSG00000117877.1023.5820.5222.05ENCSR985KAT
K562CD3EAPENSG00000117877.1023.5325.1424.34ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CD3EAP-BGKcLV15-K562ENCSR443BEFCD3EAPK562ReleasedENCSR404OHQ   CRISPRKD% = 51.4,66.4
knockdown followed by RNA-seq CD3EAP-BGKcLV28-K562ENCSR323SDLCD3EAPK562ReleasedENCSR220DTX   CRISPRKD% = 60.2,64.5
knockdown followed by RNA-seq CD3EAP-BGHcLV16-HepG2ENCSR400XYBCD3EAPHepG2ReleasedENCSR964HKT   CRISPRKD% = 52.9,52.4






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CD3EAPENCAB088EWXPA5-27632TL2692990NotSatisfied, ENCODE4BGHcLV16-11, Released, ENCODE4BGKcLV15-9, Released, ENCODE4
CD3EAPENCAB469OLRA301-294A2Released, ENCODE4BGHcLV16-11, Released, ENCODE4BGKcLV28-9, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CD3EAPK562CRISPRENCSR443BEFENCSR404OHQCD3EAP-BGKcLV15-9,CD3EAP-BGKcLV15-10NT-BGKcLV15-1,NT-BGKcLV15-251.4,66.4ReleasedENCODE4
CD3EAPK562CRISPRENCSR323SDLENCSR220DTXCD3EAP-BGKcLV28-9,CD3EAP-BGKcLV28-10NT-BGKcLV28-1,NT-BGKcLV28-260.2,64.5ReleasedENCODE4
CD3EAPHepG2CRISPRENCSR400XYBENCSR964HKTCD3EAP-BGHcLV16-11,CD3EAP-BGHcLV16-12NT-BGHcLV16-1,NT-BGHcLV16-252.9,52.4ReleasedENCODE4
CD3EAPHepG2CRISPRBGHcLV27-59,BGHcLV27-60BGHcLV27-1,BGHcLV27-213.0,17.0NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CD3EAPProduct_ID: PA5-27632
Lot_ID: TL2692990
Source: Thermo Fisher
Target Name: CD3EAP-human		 CD3EAP-HEPG2-CRISPR-PA5-7632.png<br>Caption: Western blot following CRISPR against CD3EAP in HepG2 whole cell lysate using CD3EAP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CD3EAP. CD3EAP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_PA5-27632_TL2692990_CD3EAP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CD3EAP specific antibody, PA5-27632. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the CD3EAP-specific antibody, PA5-27632. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CD3EAP-K562-CRISPRE-PA5-27632.png<br>Caption: Western blot following CRISPR against CD3EAP in K562 whole cell lysate using CD3EAP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CD3EAP. CD3EAP protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
CD3EAPProduct_ID: A301-294A
Lot_ID: 2
Source: Bethyl Labs
Target Name: CD3EAP-human		 Bethyl_A301-294A_2_CD3EAP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CD3EAP specific antibody, A301-294A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CD3EAP-specific antibody, A301-294A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CD3EAP-K562-CRISPR-A301-294A.png<br>Caption: Western blot following CRISPR against CD3EAP in K562 whole cell lysate using CD3EAP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CD3EAP. CD3EAP protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for CD3EAP is not avaliable on ENCORE database.



Proteins bound to RNA
  • CD3EAP RNA is bound by 8 proteins.  







eCLIP assay for CD3EAP is not avaliable on ENCORE database.





8 proteins are bound to CD3EAP RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr194591291045912992+3'-UTR4.041055.53799K562FAM120A
chr194591264045912764+3'-UTR,CDS4.844554.78571K562UCHL5
chr194591333345913403+3'-UTR3.706344.50550K562UPF1
chr194591345945913495+3'-UTR4.286453.20319K562UPF1
chr194591139745911531+3'-UTR,CDS4.457764.23295HepG2GRWD1
chr194591383345913910+3'-UTR3.612956.37005K562UPF1
chr194591384045913928+3'-UTR4.831306.42251K562FAM120A
chr194591384445913943+3'-UTR3.472676.21080HepG2PCBP2
chr194590997645910101+5'-UTR,CDS,intron-Distal,intron-Proximal4.464546.77156K562AQR
chr194591227345912342+CDS4.805545.86954K562UCHL5
chr194591243345912490+CDS3.294783.73423K562UCHL5
chr194591221545912273+CDS4.644585.86954K562UCHL5
chr194591203245912100+CDS3.621525.49349K562ZNF800
chr194591201845912087+CDS3.327625.07480K562PUM1
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CD3EAP is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CD3EAP is not available on ENCORE database.

  • 102 RBPs can affect the levels of CD3EAP.  








The Knockdown/knockout RNA-Seq assay for CD3EAP is not available on ENCORE database.





102 RBPs can affect the gene expression of CD3EAP :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CD3EAPENSG00000117877.6414.80630287.285100.529902.463401.95880HepG2AKAP1
CD3EAPENSG00000117877.6297.19760510.76340-0.781209.315208.17880HepG2AUH
CD3EAPENSG00000117877.6124.66260293.53530-1.235508.485007.02330HepG2BCLAF1
CD3EAPENSG00000117877.6336.99750621.10540-0.882109.290408.27530HepG2BOP1
CD3EAPENSG00000117877.6273.07450506.98410-0.892606.367704.86660HepG2CCAR1
CD3EAPENSG00000117877.6138.30240225.83360-0.707403.144102.32170HepG2CIRBP
CD3EAPENSG00000117877.6246.55790570.19490-1.2095013.8037012.40650HepG2CPSF6
CD3EAPENSG00000117877.6467.61550347.218000.429402.975702.31270HepG2CELF1
CD3EAPENSG00000117877.6259.70450373.37870-0.523804.230203.34400HepG2DAZAP1
CD3EAPENSG00000117877.6124.61850206.09280-0.725803.456102.70510HepG2DDX21
CD3EAPENSG00000117877.6142.36850275.19090-0.950805.650504.78630HepG2DDX3X
CD3EAPENSG00000117877.6343.21670647.50270-0.915805.417404.06320HepG2DDX59
CD3EAPENSG00000117877.6247.09640342.02700-0.469002.388001.44600HepG2DDX5
CD3EAPENSG00000117877.6136.70910236.41010-0.790203.543402.85240HepG2DDX6
CD3EAPENSG00000117877.6122.17660210.14350-0.782404.020402.85250HepG2DHX30
CD3EAPENSG00000117877.6172.14820278.13660-0.692104.397203.57950HepG2DKC1
CD3EAPENSG00000117877.6137.07800217.87290-0.668503.515702.73000HepG2DNAJC21
CD3EAPENSG00000117877.677.26840227.25140-1.5563010.596909.48350HepG2EIF2S1
CD3EAPENSG00000117877.6435.96970310.760300.488303.012801.78560HepG2EIF2S2
CD3EAPENSG00000117877.6179.82700251.13210-0.481801.838101.31300HepG2EIF4A3
CD3EAPENSG00000117877.6383.20570244.526500.648003.008702.29410HepG2EIF4B
CD3EAPENSG00000117877.6228.93950352.84340-0.624103.839802.89130HepG2EIF4G2
CD3EAPENSG00000117877.6458.46740586.44910-0.355202.744602.14600HepG2ETF1
CD3EAPENSG00000117877.6433.25580284.053000.609004.496203.43770HepG2EWSR1
CD3EAPENSG00000117877.6330.60150571.20070-0.788902.937701.88990HepG2FTO
CD3EAPENSG00000117877.6253.85780646.32290-1.3482015.8795014.46600HepG2GPKOW
CD3EAPENSG00000117877.6122.00280219.96040-0.850304.085903.32390HepG2HNRNPA1
CD3EAPENSG00000117877.6234.34840343.12930-0.550103.797702.73060HepG2HNRNPA2B1
CD3EAPENSG00000117877.6123.76600244.67270-0.983205.629004.66700HepG2HNRNPC
CD3EAPENSG00000117877.6346.44640620.64180-0.8411011.4456010.12160HepG2HNRNPF
CD3EAPENSG00000117877.6363.77960238.044800.611703.425302.65100HepG2HNRNPK
CD3EAPENSG00000117877.6181.55570616.80120-1.7644025.8662024.26350HepG2HNRNPLL
CD3EAPENSG00000117877.6275.89930611.16000-1.1474016.1229014.53340HepG2IGF2BP1
CD3EAPENSG00000117877.6486.97070254.907900.933807.081306.10730HepG2MATR3
CD3EAPENSG00000117877.6239.39290629.13900-1.3940012.7152010.61910HepG2MTPAP
CD3EAPENSG00000117877.6259.03510546.93950-1.0782010.910709.51000HepG2NSUN2
CD3EAPENSG00000117877.6181.19730112.729800.684602.177701.40440HepG2PABPC1
CD3EAPENSG00000117877.6335.93190209.173700.683405.327404.52910HepG2PCBP1
CD3EAPENSG00000117877.6178.78650272.61770-0.608602.520801.52970HepG2PCBP2
CD3EAPENSG00000117877.6197.15660110.602100.833903.401501.70600HepG2PNPT1
CD3EAPENSG00000117877.6118.38160256.21760-1.113906.790105.58710HepG2PRPF8
CD3EAPENSG00000117877.6189.24630353.41680-0.901107.177806.27420HepG2PUF60
CD3EAPENSG00000117877.6582.53370375.156400.634707.729906.84250HepG2PUM1
CD3EAPENSG00000117877.6180.49110296.11920-0.714305.473704.66040HepG2PUS1
CD3EAPENSG00000117877.6139.75150209.20490-0.582102.477801.83570HepG2RBM25
CD3EAPENSG00000117877.6408.44030288.974600.499102.565501.61280HepG2RBM27
CD3EAPENSG00000117877.6359.71680668.50830-0.894109.541308.39730HepG2RPLP0
CD3EAPENSG00000117877.6133.32200258.46600-0.955104.840403.60680HepG2RPS10
CD3EAPENSG00000117877.6201.48940116.648700.788403.444001.99320HepG2SART3
CD3EAPENSG00000117877.6380.75850610.48970-0.681105.764804.77710HepG2SF3B1
CD3EAPENSG00000117877.6402.60860252.405800.673504.706303.90420HepG2SMN1
CD3EAPENSG00000117877.6299.22830679.28460-1.182809.898708.42660HepG2SNRNP70
CD3EAPENSG00000117877.6359.62080553.60620-0.622406.787306.09930HepG2SRFBP1
CD3EAPENSG00000117877.6452.40740656.95060-0.538203.088302.02270HepG2SRSF3
CD3EAPENSG00000117877.6315.12650609.74270-0.9523014.0803012.71960HepG2SRSF7
CD3EAPENSG00000117877.6177.54900480.64560-1.4368023.8659022.68830HepG2SSB
CD3EAPENSG00000117877.6349.56390238.015200.554402.893101.58160HepG2SSRP1
CD3EAPENSG00000117877.6632.69140396.845800.672808.537907.54600HepG2SUCLG1
CD3EAPENSG00000117877.687.91690330.64570-1.9111017.5354016.08620HepG2SUPV3L1
CD3EAPENSG00000117877.6133.60620207.90960-0.638003.003502.42310HepG2TAF15
CD3EAPENSG00000117877.6266.59060601.28960-1.1734014.0565012.62700HepG2TARDBP
CD3EAPENSG00000117877.6123.19060236.14520-0.938806.145805.45350HepG2TIAL1
CD3EAPENSG00000117877.6466.45070353.854200.398501.960601.42200HepG2U2AF1
CD3EAPENSG00000117877.6222.44620358.30110-0.687705.722704.77570HepG2UPF1
CD3EAPENSG00000117877.6184.96200302.76450-0.711003.235602.14630HepG2UPF2
CD3EAPENSG00000117877.6508.61140345.925900.556005.066304.29680HepG2UTP3
CD3EAPENSG00000117877.6223.65520325.48450-0.541302.451901.76540HepG2XRN2
CD3EAPENSG00000117877.6196.31940359.55890-0.873004.934104.08080HepG2ZC3H8
CD3EAPENSG00000117877.6253.07320394.06290-0.638905.953905.23120HepG2ZNF622
CD3EAPENSG00000117877.6485.16240818.96960-0.755307.511006.04610K562BOP1
CD3EAPENSG00000117877.6551.50620870.82970-0.659007.697906.37990K562CPSF6
CD3EAPENSG00000117877.61689.106001103.123500.614607.052906.07890K562DDX47
CD3EAPENSG00000117877.6411.03900267.004300.622304.931203.71050K562DKC1
CD3EAPENSG00000117877.6609.311401189.55180-0.9652021.5804019.86120K562EIF2S2
CD3EAPENSG00000117877.6602.37380427.199200.495702.187901.30590K562EIF3A
CD3EAPENSG00000117877.6622.25730259.151001.2636021.2616019.00290K562EIF3G
CD3EAPENSG00000117877.6367.07550223.778300.713905.123204.00530K562EIF4A3
CD3EAPENSG00000117877.6292.91730201.519300.539503.358902.06310K562EIF4B
CD3EAPENSG00000117877.6163.72630277.24610-0.759904.333102.29000K562FASTKD2
CD3EAPENSG00000117877.6717.181201100.40530-0.617603.322402.38780K562FIP1L1
CD3EAPENSG00000117877.6487.09830885.99050-0.8631010.441008.72270K562FMR1
CD3EAPENSG00000117877.6513.93550750.93160-0.547104.193703.04840K562FTO
CD3EAPENSG00000117877.6307.15260211.111700.540803.410201.40640K562FUBP3
CD3EAPENSG00000117877.6119.79000229.12710-0.935604.522702.75880K562FUS
CD3EAPENSG00000117877.61034.63460232.719102.1524071.3871068.61860K562FXR1
CD3EAPENSG00000117877.662.56180136.92170-1.130004.567003.60770K562G3BP2
CD3EAPENSG00000117877.6497.81090831.45210-0.740007.882606.53500K562GPKOW
CD3EAPENSG00000117877.6208.00370415.91840-0.999704.575702.88110K562HNRNPA2B1
CD3EAPENSG00000117877.6251.84810570.57440-1.1799014.7838013.48740K562HNRNPC
CD3EAPENSG00000117877.61102.728101492.03400-0.436202.247401.52650K562HSPD1
CD3EAPENSG00000117877.6467.95670709.89350-0.601203.698602.60100K562KRR1
CD3EAPENSG00000117877.6533.17500249.760901.0940014.1886012.16760K562MAGOH
CD3EAPENSG00000117877.6509.72180249.248701.0320012.5812010.49280K562MATR3
CD3EAPENSG00000117877.6614.81820811.57990-0.400602.210801.42780K562METAP2
CD3EAPENSG00000117877.6761.36740954.95540-0.326802.289101.60430K562NSUN2
CD3EAPENSG00000117877.6221.00450363.92940-0.719602.467601.54640K562PABPN1
CD3EAPENSG00000117877.6379.55550222.218700.772205.731104.31450K562PCBP2
CD3EAPENSG00000117877.6625.98780998.14210-0.673106.245604.99270K562PCBP3
CD3EAPENSG00000117877.6514.29790759.22730-0.561904.442602.38520K562PKM
CD3EAPENSG00000117877.6608.45470765.56500-0.331402.375901.63470K562POLR2G
CD3EAPENSG00000117877.6340.41980511.56990-0.587604.340003.16900K562PSIP1
CD3EAPENSG00000117877.6764.89560242.661001.6562013.9195012.49130K562PTBP1
CD3EAPENSG00000117877.61843.552501269.541600.538102.567701.78290K562PUF60
CD3EAPENSG00000117877.61866.601501349.569700.467802.256701.30360K562PUM1
CD3EAPENSG00000117877.6344.87150232.663200.567703.459202.51400K562RBM22
CD3EAPENSG00000117877.6391.78850244.845400.678103.710502.42430K562NELFE
CD3EAPENSG00000117877.61200.96850682.275200.815703.198002.03470K562SAFB
CD3EAPENSG00000117877.6596.52240790.65300-0.406502.324601.44180K562SF3B1
CD3EAPENSG00000117877.6261.56250142.388600.877206.376305.04130K562SF3B4
CD3EAPENSG00000117877.6146.49860237.95680-0.699803.393501.60890K562SND1
CD3EAPENSG00000117877.6370.94330237.892800.640804.420703.53130K562SRSF1
CD3EAPENSG00000117877.6197.01750457.24220-1.214605.951603.62940K562SUPT6H
CD3EAPENSG00000117877.61416.24700767.942500.8829017.0906015.08310K562SYNCRIP
CD3EAPENSG00000117877.6347.34530240.754200.528702.902202.17740K562TARDBP
CD3EAPENSG00000117877.6367.50380492.05040-0.421002.181501.36060K562TIAL1
CD3EAPENSG00000117877.6889.705601446.94050-0.701607.168105.09650K562TROVE2
CD3EAPENSG00000117877.6356.09060581.51070-0.707605.670404.16070K562UCHL5
CD3EAPENSG00000117877.6513.165301012.55170-0.9805010.019808.76250K562UPF1
CD3EAPENSG00000117877.6447.00100866.42110-0.954804.492103.36390K562WDR3
CD3EAPENSG00000117877.6198.27430389.45030-0.973904.040001.91410K562XPO5
CD3EAPENSG00000117877.6234.12670449.86580-0.942203.923001.88680K562YTHDC2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CD3EAP is not available on ENCORE database.





1 RBPs can affect the splicing of CD3EAP :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
A5SSCD3EAPENSG00000117877.6chr19+4591035145910578459103514591049345911390459140240.172000.000040.00116longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CD3EAP is not avaliable on ENCORE database.