MANF

General Information

Gene Name       : MANF
Gene ID             : ENSG00000145050.16
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: MANF)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878MANFENSG00000145050.1679.1481.6280.38ENCSR000AEF
HepG2MANFENSG00000145050.16182.54196.84189.69ENCSR985KAT
K562MANFENSG00000145050.1637.0435.4636.25ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  

No Antibody Primary Validation image or Secondary image available



  KD RNA-Seq  

No KD RNA-Seq experiment available



  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela








RNA Targets

  • eCLIP assay for MANF is not avaliable on ENCORE database.



Proteins bound to RNA
  • MANF RNA is bound by 26 proteins.  







eCLIP assay for MANF is not avaliable on ENCORE database.





26 proteins are bound to MANF RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr35142661051426621+3'-UTR4.7438511.09831HepG2DDX55
chr35142660051426692+3'-UTR4.0964310.40043K562DDX6
chr35142662451426657+3'-UTR3.4192718.28994K562TIA1
chr35142638851426448+3'-UTR,CDS4.482203.83751K562SND1
chr35142639251426443+3'-UTR,CDS3.832256.34803HepG2LARP4
chr35142667351426726+3'-UTR3.8637814.32705K562TIA1
chr35142638551426469+3'-UTR,CDS3.084685.06369HepG2GRWD1
chr35142516851425216+3'-UTR,CDS3.858999.05969K562YBX3
chr35142659951426610+3'-UTR5.0932611.88205HepG2DDX55
chr35142652551426562+3'-UTR4.289508.89412HepG2LARP4
chr35142660551426624+3'-UTR4.1476215.39771K562TIA1
chr35142633751426460+3'-UTR,CDS4.2260113.42460HepG2BCLAF1
chr35142660051426631+3'-UTR5.301908.65456HepG2TIAL1
chr35142660151426702+3'-UTR4.872556.08097HepG2TIA1
chr35142525551425302+3'-UTR,CDS4.916556.37937HepG2BCLAF1
chr35142633451426375+3'-UTR,CDS,intron-Proximal3.305675.95143HepG2LIN28B
chr35142522451425277+3'-UTR,CDS3.983553.30795HepG2SND1
chr35142659851426686+3'-UTR3.930667.75227HepG2DDX6
chr35142659751426716+3'-UTR3.044714.77070HepG2ZC3H11A
chr35142647351426525+3'-UTR,CDS4.3201313.09540HepG2LARP4
chr35142654451426596+3'-UTR3.593923.68294K562PUM2
chr35142629651426372+3'-UTR,CDS,intron-Proximal3.4352810.23609HepG2BUD13
chr35142662451426734+3'-UTR4.9604245.34070HepG2LARP4
chr35142639151426448+3'-UTR,CDS5.3197612.50669HepG2SND1
chr35142663451426681+3'-UTR3.998965.12374K562PABPC4
chr35142660051426621+3'-UTR4.9107633.14418HepG2LARP4
chr35142527951425312+3'-UTR,CDS,intron-Proximal4.653376.67079HepG2LIN28B
chr35142660051426679+3'-UTR4.974399.05620HepG2SUB1
chr35142625551426388+3'-UTR,CDS,intron-Proximal3.194726.41819HepG2AQR
chr35142516751425215+3'-UTR,CDS,intron-Proximal3.435974.76748HepG2SND1
chr35142527051425308+3'-UTR,CDS4.531613.56019HepG2GRWD1
chr35142637551426425+3'-UTR,CDS3.805679.10188HepG2LIN28B
chr35142516751425250+3'-UTR,CDS,intron-Proximal3.107183.99689HepG2GRWD1
chr35142516951425295+3'-UTR,CDS3.780005.13799K562SND1
chr35142639651426452+3'-UTR,CDS3.233824.86973K562UCHL5
chr35142663151426680+3'-UTR4.412496.12154HepG2TIAL1
chr35142633451426349+3'-UTR,CDS,intron-Proximal4.319343.31827HepG2GRWD1
chr35142659651426648+3'-UTR3.798796.74304K562PUM2
chr35142633751426391+3'-UTR,CDS5.339655.84094HepG2SND1
chr35142276151422823+5'-UTR,CDS4.1686317.61238K562DROSHA
chr35142275451422863+5'-UTR,CDS3.031554.00925HepG2EIF3H
chr35142275651422876+5'-UTR,CDS,intron-Proximal5.4699317.42561HepG2PRPF4
chr35142276351422828+5'-UTR,CDS5.9921937.41210K562FTO
chr35142276551422824+5'-UTR,CDS3.108503.20426K562DDX6
chr35142276451422823+5'-UTR,CDS3.7855215.61126HepG2RPS3
chr35142276151422866+5'-UTR,CDS3.0484521.01714HepG2NCBP2
chr35142276451422865+5'-UTR,CDS3.672108.88489K562UCHL5
chr35142274551422820+5'-UTR,CDS5.28214400.00000HepG2DDX3X
chr35142365951423713+CDS3.178075.79905HepG2BCLAF1
chr35142282351422866+CDS5.49333400.00000HepG2DDX3X
chr35142370651423767+CDS,intron-Proximal3.355414.91547HepG2GRWD1
chr35142286151422915+CDS,intron-Proximal4.7205310.26986HepG2PRPF8
chr35142372051423802+CDS,intron-Proximal3.406473.39008HepG2AQR
chr35142281951422865+CDS3.896055.53636HepG2GTF2F1
chr35142282851422867+CDS7.0783528.26180K562FTO
chr35142282351422854+CDS5.678783.42986HepG2RPS3
chr35142364651423706+CDS4.556575.79466HepG2RPS3
chr35142282351422866+CDS4.673968.79778K562DROSHA
chr35142282451422865+CDS3.983283.90326K562DDX6
chr35142286751422920+intron-Proximal4.422564.92499HepG2AQR
chr35142377751423813+intron-Proximal4.065707.90583K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for MANF is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for MANF is not available on ENCORE database.

  • 130 RBPs can affect the levels of MANF.  








The Knockdown/knockout RNA-Seq assay for MANF is not available on ENCORE database.





130 RBPs can affect the gene expression of MANF :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
MANFENSG00000145050.11709.888401055.91710-0.572805.409104.08170HepG2ADAR
MANFENSG00000145050.11819.398401132.12080-0.466406.476305.34990HepG2AKAP8L
MANFENSG00000145050.11933.021201996.39460-1.0974022.8172021.36030HepG2BOP1
MANFENSG00000145050.112614.571301502.137300.799508.084106.86480HepG2CALR
MANFENSG00000145050.111322.854902117.23290-0.678506.079104.60780HepG2CCAR1
MANFENSG00000145050.11833.827001126.57170-0.434106.589305.64330HepG2CKAP4
MANFENSG00000145050.111683.893701384.250900.282602.463401.42330HepG2CPSF7
MANFENSG00000145050.111288.547301082.800000.250902.208501.30140HepG2YBX3
MANFENSG00000145050.112411.499201975.251400.287804.923603.97550HepG2DAZAP1
MANFENSG00000145050.11775.981101042.17120-0.425506.208605.03100HepG2DDX19B
MANFENSG00000145050.11616.482901107.62220-0.8453011.9968010.70240HepG2DDX21
MANFENSG00000145050.11950.000901256.94890-0.403902.291101.58170HepG2DDX55
MANFENSG00000145050.111221.166202081.25140-0.769205.138203.81770HepG2DDX59
MANFENSG00000145050.11705.829601025.53250-0.539002.905601.90410HepG2DHX30
MANFENSG00000145050.11778.697301078.09910-0.469406.138605.20570HepG2DKC1
MANFENSG00000145050.11862.912801063.30770-0.301302.736802.03610HepG2DNAJC21
MANFENSG00000145050.111008.958901275.06400-0.337702.727501.56880HepG2EIF2S2
MANFENSG00000145050.11556.177201045.96020-0.9112014.9886012.89000HepG2EIF3G
MANFENSG00000145050.11983.236101948.65030-0.9869032.0206029.85100HepG2EIF4G1
MANFENSG00000145050.111150.313201455.99430-0.340002.383501.36840HepG2ESF1
MANFENSG00000145050.11887.347401468.76160-0.7270015.8923014.64650HepG2ETF1
MANFENSG00000145050.11751.908901055.51660-0.489304.788503.34710HepG2FASTKD2
MANFENSG00000145050.111201.807901697.09270-0.497903.527502.66560HepG2FIP1L1
MANFENSG00000145050.111145.23830911.178900.329702.086101.48210HepG2FXR1
MANFENSG00000145050.111645.943102077.49260-0.335902.254601.67340HepG2GPKOW
MANFENSG00000145050.11628.240901073.49320-0.7729010.079808.96140HepG2HNRNPA1
MANFENSG00000145050.11283.88290410.93810-0.533602.688401.30800HepG2HNRNPAB
MANFENSG00000145050.11891.955901194.02450-0.420803.596202.81360HepG2HNRNPC
MANFENSG00000145050.111137.738902092.17110-0.8788026.5040024.62090HepG2HNRNPF
MANFENSG00000145050.111501.80100855.973100.8110011.219509.89910HepG2HNRNPK
MANFENSG00000145050.111066.302601982.59760-0.8948013.7716012.51230HepG2HNRNPLL
MANFENSG00000145050.11785.872101186.13310-0.593908.752907.72110HepG2HNRNPU
MANFENSG00000145050.111215.02400762.455900.672205.424003.72740HepG2HSPD1
MANFENSG00000145050.111217.105102060.20250-0.7593010.017708.72300HepG2IGF2BP1
MANFENSG00000145050.111153.45360859.098500.425003.143902.42980HepG2IGF2BP2
MANFENSG00000145050.11304.07920485.28170-0.674403.722202.16170HepG2ILF3
MANFENSG00000145050.11709.876001030.06880-0.537109.129507.37370HepG2KIF1C
MANFENSG00000145050.111217.83830761.932700.676505.002703.32270HepG2KRR1
MANFENSG00000145050.111560.99260911.497600.776109.219008.03380HepG2MAGOH
MANFENSG00000145050.111299.41850916.580700.503403.733903.01080HepG2MATR3
MANFENSG00000145050.111212.373102022.18970-0.738105.794904.36700HepG2MTPAP
MANFENSG00000145050.11684.95430938.15630-0.453802.992902.33520HepG2NCBP2
MANFENSG00000145050.11587.83760792.78980-0.431503.410901.84260HepG2NIP7
MANFENSG00000145050.111209.33590722.571200.742905.782503.71890HepG2NKRF
MANFENSG00000145050.11723.716001154.05810-0.673203.214602.03020HepG2NONO
MANFENSG00000145050.111165.902801758.01770-0.592506.667405.53270HepG2NSUN2
MANFENSG00000145050.111444.971502955.56160-1.032403.407101.37270HepG2NUFIP2
MANFENSG00000145050.11703.13230454.951700.628003.089402.14920HepG2PABPC1
MANFENSG00000145050.111394.19470907.273900.6197011.7048010.59480HepG2PCBP1
MANFENSG00000145050.11905.692401316.76650-0.539903.278602.15110HepG2PCBP2
MANFENSG00000145050.11543.45360746.31320-0.457604.131302.61890HepG2PKM
MANFENSG00000145050.11770.752501009.43830-0.389204.451803.49520HepG2PRPF4
MANFENSG00000145050.11850.631201384.22780-0.702504.977803.37760HepG2PRPF6
MANFENSG00000145050.11381.13520969.79530-1.3474020.0966018.21440HepG2PRPF8
MANFENSG00000145050.11893.848601869.68620-1.0647023.2790021.77520HepG2PUF60
MANFENSG00000145050.111229.013501984.73310-0.6914022.0587020.71270HepG2PUM1
MANFENSG00000145050.112739.861301983.296600.466106.929305.88610HepG2PUM2
MANFENSG00000145050.11725.659501147.78700-0.6615013.0356011.86810HepG2PUS1
MANFENSG00000145050.11389.50590999.87020-1.3601031.0773029.33560HepG2QKI
MANFENSG00000145050.11677.014001270.64520-0.908306.183505.15500HepG2RAVER1
MANFENSG00000145050.111372.929101723.71020-0.328302.312401.51990HepG2RBM15
MANFENSG00000145050.11702.437001227.61210-0.8054015.0896014.07770HepG2RBM22
MANFENSG00000145050.11641.400502253.61160-1.8129057.9151055.78920HepG2RPLP0
MANFENSG00000145050.11720.976501248.27960-0.791906.062804.69840HepG2RPS10
MANFENSG00000145050.11458.82360878.17140-0.9366013.7049012.46960HepG2RPS19
MANFENSG00000145050.11556.62290975.08980-0.808809.817408.38950HepG2RPS2
MANFENSG00000145050.11519.51740959.34410-0.884908.717407.42840HepG2RRP9
MANFENSG00000145050.111287.218801056.326500.285103.332102.63610HepG2SAFB2
MANFENSG00000145050.11684.94140881.30920-0.363702.448901.56260HepG2SERBP1
MANFENSG00000145050.11740.904401962.32780-1.4052034.2694032.12580HepG2SF3B1
MANFENSG00000145050.11687.78560961.18210-0.482905.688104.89140HepG2SF3B4
MANFENSG00000145050.111178.014901792.69370-0.6058015.4753014.06180HepG2SLBP
MANFENSG00000145050.11703.718501239.85520-0.817103.680301.84520HepG2SLTM
MANFENSG00000145050.11938.528101231.89320-0.392403.716002.98870HepG2SMN1
MANFENSG00000145050.11745.172101236.86610-0.731006.191203.76890HepG2SND1
MANFENSG00000145050.111037.267101386.54120-0.418706.024405.36390HepG2SRFBP1
MANFENSG00000145050.111308.320801003.239500.383004.637004.06430HepG2SRSF1
MANFENSG00000145050.111386.768902111.62000-0.606605.097303.70090HepG2SRSF3
MANFENSG00000145050.111441.419901846.01720-0.356905.269704.44780HepG2SRSF5
MANFENSG00000145050.111136.345702055.44990-0.8551025.0909023.40530HepG2SRSF7
MANFENSG00000145050.11904.628901203.84350-0.412205.618704.93780HepG2SSB
MANFENSG00000145050.11295.02170434.67160-0.559103.056501.89710HepG2STIP1
MANFENSG00000145050.111418.741601146.111400.307802.936801.96950HepG2SUPT6H
MANFENSG00000145050.11601.861101251.31990-1.0559012.8328011.55470HepG2SUPV3L1
MANFENSG00000145050.11748.84500901.67720-0.267901.824401.35110HepG2TAF15
MANFENSG00000145050.111117.230501932.65880-0.7907012.0483010.69790HepG2TARDBP
MANFENSG00000145050.111229.04390746.333100.719503.213802.06540HepG2TBRG4
MANFENSG00000145050.11434.639701152.69320-1.4071035.4551034.03490HepG2TIAL1
MANFENSG00000145050.111776.195301162.843700.611007.820606.95050HepG2TRIM56
MANFENSG00000145050.11506.92650717.07580-0.500303.111401.45710HepG2TROVE2
MANFENSG00000145050.111458.299401826.56930-0.324803.066702.14120HepG2TUFM
MANFENSG00000145050.111179.921601451.85420-0.299201.992701.45030HepG2U2AF1
MANFENSG00000145050.111339.449201933.37190-0.529504.604303.50580HepG2UBE2L3
MANFENSG00000145050.11486.01810739.22500-0.605004.759003.75740HepG2UCHL5
MANFENSG00000145050.112097.587501564.761000.422702.472301.47330HepG2UTP18
MANFENSG00000145050.11980.875701469.75550-0.5834011.0774010.00750HepG2UTP3
MANFENSG00000145050.11900.269001249.60370-0.473002.735001.48060HepG2XRCC5
MANFENSG00000145050.11945.609001231.78580-0.381402.332301.66240HepG2XRN2
MANFENSG00000145050.111428.206801674.27730-0.229302.962202.40230HepG2ZNF622
MANFENSG00000145050.11707.432001269.59870-0.843707.855705.55600HepG2ZRANB2
MANFENSG00000145050.11622.22530866.11050-0.477103.761202.31050K562ABCF1
MANFENSG00000145050.113211.057902170.539500.564902.494801.95090K562AGGF1
MANFENSG00000145050.111261.08620922.609300.450805.200203.07830K562AKAP1
MANFENSG00000145050.113670.748902385.631400.621602.980301.94900K562AKAP8
MANFENSG00000145050.113616.610802258.676800.679103.307802.59190K562AKAP8L
MANFENSG00000145050.113885.442102370.145100.713003.857802.76390K562ATP5C1
MANFENSG00000145050.11434.65920570.83520-0.393202.156801.39310K562BOP1
MANFENSG00000145050.11384.24990603.28630-0.650804.757802.82520K562BUD13
MANFENSG00000145050.11805.862301672.02490-1.053002.363901.39580K562CNOT7
MANFENSG00000145050.11410.91130606.99280-0.562902.790702.00470K562CPSF6
MANFENSG00000145050.111466.57350766.398500.936205.618602.91770K562DAZAP1
MANFENSG00000145050.111357.73950990.611300.454706.331203.70010K562DDX24
MANFENSG00000145050.111206.26760934.895200.367603.443802.26560K562DDX3X
MANFENSG00000145050.113733.386302429.670200.619602.868201.97520K562DKC1
MANFENSG00000145050.11271.00280439.85680-0.698705.166303.31750K562DNAJC21
MANFENSG00000145050.11286.39580475.62440-0.731807.260405.98100K562EIF3G
MANFENSG00000145050.11829.853101246.47480-0.586902.641101.72130K562EIF4G2
MANFENSG00000145050.11424.07460661.98530-0.642504.357302.26890K562ESF1
MANFENSG00000145050.11370.09730554.51830-0.583304.998603.24720K562EXOSC5
MANFENSG00000145050.11703.85240508.838500.468003.409601.61910K562FASTKD2
MANFENSG00000145050.11341.82090617.54030-0.853307.625906.16390K562FMR1
MANFENSG00000145050.11549.314401015.79880-0.8869016.5767015.20480K562FXR1
MANFENSG00000145050.111328.55820970.560200.452903.014501.51020K562G3BP1
MANFENSG00000145050.11446.25600579.55380-0.377102.085301.39230K562GPKOW
MANFENSG00000145050.11388.19150663.57000-0.773508.015707.04840K562HNRNPC
MANFENSG00000145050.11417.23920592.53360-0.506003.693802.35510K562HNRNPF
MANFENSG00000145050.11711.83330512.090800.475002.990502.01900K562HNRNPM
MANFENSG00000145050.11567.73250721.23830-0.345302.676001.79800K562HNRNPU
MANFENSG00000145050.111266.41030844.100400.585206.217405.03640K562HSPD1
MANFENSG00000145050.111548.694601014.855800.609705.362803.73800K562IGF2BP2
MANFENSG00000145050.111607.772001032.385100.639005.551403.75310K562IGF2BP3
MANFENSG00000145050.11329.40990494.80110-0.587003.049802.06510K562KRR1
MANFENSG00000145050.11629.857001066.91810-0.7604010.754609.02880K562MAGOH
MANFENSG00000145050.113472.364902294.470900.597702.820801.73320K562MAK16
MANFENSG00000145050.11776.336001064.80600-0.455804.568903.35490K562MATR3
MANFENSG00000145050.11400.94670565.69860-0.496602.922402.02070K562METAP2
MANFENSG00000145050.111365.50680975.317400.485405.971604.18570K562NCBP2
MANFENSG00000145050.11557.86880869.11600-0.6396010.014108.71530K562NSUN2
MANFENSG00000145050.11678.703201606.31430-1.242903.150001.82280K562PABPC1
MANFENSG00000145050.111383.557701042.746800.407904.272802.56600K562PCBP1
MANFENSG00000145050.11495.39230949.35580-0.9384013.1952010.94660K562PCBP2
MANFENSG00000145050.11491.88310705.34520-0.520003.497402.56240K562PCBP3
MANFENSG00000145050.11401.11790541.02480-0.431702.767001.96510K562POLR2G
MANFENSG00000145050.111062.36760706.765100.587905.874604.60450K562PPIG
MANFENSG00000145050.11923.25300594.950200.633902.465201.61770K562PSIP1
MANFENSG00000145050.11422.380901036.55200-1.2952028.4140026.38030K562PTBP1
MANFENSG00000145050.111008.986601447.17080-0.520302.305601.33980K562PUM1
MANFENSG00000145050.11673.42720994.11770-0.561905.610804.36400K562RBM22
MANFENSG00000145050.11521.806201046.10590-1.0034012.4890010.17290K562NELFE
MANFENSG00000145050.11550.19680931.60380-0.759807.339605.61150K562RPS3
MANFENSG00000145050.11320.26760482.06310-0.589904.269902.91380K562SAFB
MANFENSG00000145050.11296.28770498.39870-0.750306.247704.10530K562SDAD1
MANFENSG00000145050.11507.85250857.70260-0.756109.912808.43880K562SF3A3
MANFENSG00000145050.11324.65170551.08520-0.763405.682704.25630K562SF3B1
MANFENSG00000145050.11822.311801169.25010-0.507803.458402.49830K562SF3B4
MANFENSG00000145050.11727.082101016.12790-0.482904.792003.86670K562SRSF1
MANFENSG00000145050.111242.72110959.560500.373002.959201.86620K562SRSF5
MANFENSG00000145050.11420.41430542.66060-0.368202.435801.66590K562SYNCRIP
MANFENSG00000145050.11545.047901028.47100-0.9160012.3542010.67080K562TARDBP
MANFENSG00000145050.11344.10050572.24830-0.733803.787002.68720K562TIAL1
MANFENSG00000145050.11663.27270820.04360-0.306102.044401.30950K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for MANF is not available on ENCORE database.





2 RBPs can affect the splicing of MANF :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
A3SSMANFENSG00000145050.11chr3+514251675142530951425189514253095142283951423766-0.059000.000000.00000shorterK562SF3B4
A3SSMANFENSG00000145050.11chr3+5142514451425309514251675142530951422839514237660.054000.000000.00000longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for MANF is not avaliable on ENCORE database.