RTCB

General Information

Gene Name       : RTCB
Gene ID             : ENSG00000100220.11
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RTCB)


Antibody Validation

 View validation images for all RTCB antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RTCBENSG00000100220.1173.1369.5571.34ENCSR000AEF
HepG2RTCBENSG00000100220.11102.0295.2098.61ENCSR985KAT
K562RTCBENSG00000100220.1166.8663.5365.19ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RTCB-BGHcLV31-HepG2ENCSR726RTMRTCBHepG2ReleasedENCSR452TFV   CRISPRKD% = 70.4,75.7






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RTCBENCAB377OYKA305-078A1Released, ENCODE4BGHcLV31-17, Released, ENCODE4BGKcLV35-63, Released, ENCODE4
RTCBENCAB418OYGA305-079A1Released, ENCODE4BGHcLV31-17, Released, ENCODE4BGKcLV35-63, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RTCBK562CRISPRBGKcLV33-15,BGKcLV33-16BGKcLV33-1,BGKcLV33-277.5,79.3NotSatisfiedENCODE4
RTCBK562CRISPRBGKcLV33-21,BGKcLV33-22BGKcLV33-1,BGKcLV33-20.0,0.0NotSatisfiedENCODE4
RTCBK562CRISPRRTCB-BGKcLV35-63,RTCB-BGKcLV35-64NT-BGKcLV35-1,NT-BGKcLV35-252.9,57.1SubmittingENCORE2
RTCBHepG2CRISPRENCSR726RTMENCSR452TFVRTCB-BGHcLV31-17,RTCB-BGHcLV31-18NT-BGHcLV31-1,NT-BGHcLV31-270.4,75.7ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RTCBProduct_ID: A305-078A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RTCB-human		 RTCB-HEPG2-CRISPR-A305-078A.png<br>Caption: Western blot following CRISPR against RTCB in HepG2 whole cell lysate using RTCB specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RTCB. RTCB protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A305-078A_1_RTCB.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RTCB specific antibody, A305-078A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RTCB-specific antibody, A305-078A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RTCB-K562-CRISPR-A305-078A.png<br>Caption: Western blot following CRISPR against RTCB in K562 whole cell lysate using RTCB specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RTCB. RTCB protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RTCBProduct_ID: A305-079A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RTCB-human		 Bethyl_A305-079A_1_RTCB.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RTCB specific antibody, A305-079A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RTCB-specific antibody, A305-079A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RTCB-K562-CRISPR-A305-079A.png<br>Caption: Western blot following CRISPR against RTCB in K562 whole cell lysate using RTCB specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RTCB. RTCB protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for RTCB is not avaliable on ENCORE database.



Proteins bound to RNA
  • RTCB RNA is bound by 13 proteins.  







eCLIP assay for RTCB is not avaliable on ENCORE database.





13 proteins are bound to RTCB RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr223278393332783993-3'-UTR,CDS,intron-Distal4.256018.19301K562PABPC4
chr223278382132783900-3'-UTR,intron-Distal4.229678.02236HepG2LIN28B
chr223278375732783819-3'-UTR,intron-Distal4.385065.84664K562PUM2
chr223278382132783878-3'-UTR,intron-Distal4.5568912.71765K562LIN28B
chr223278363632783704-3'-UTR,intron-Distal5.0334910.44987K562PABPC4
chr223278367232783728-3'-UTR,intron-Distal5.634985.74792HepG2SUB1
chr223280808832808216-5'-UTR,CDS,intron-Distal4.0311315.52136K562DDX3X
chr223279114732791223-CDS,intron-Distal,intron-Proximal3.942344.15711K562AQR
chr223280412932804195-CDS,intron-Distal,intron-Proximal4.379548.11807HepG2PRPF8
chr223279392732794004-CDS,intron-Distal,intron-Proximal3.814884.79276K562ZNF622
chr223279392932794022-CDS,intron-Distal,intron-Proximal3.749289.04169HepG2G3BP1
chr223280416832804221-CDS,intron-Distal4.390174.69712HepG2G3BP1
chr223280418732804230-CDS,intron-Distal,intron-Proximal3.353485.15500HepG2BCLAF1
chr223280805232808088-CDS,intron-Distal3.443963.92957K562DDX3X
chr223279197532792063-CDS,intron-Distal,intron-Proximal4.264275.97994K562AQR
chr223279393332793999-CDS,intron-Distal,intron-Proximal3.463304.40383K562UCHL5
chr223279405232794083-CDS,intron-Distal3.266953.50786HepG2G3BP1
chr223280799232808087-CDS,intron-Distal5.672029.06713HepG2PRPF8
chr223280267932802742-CDS,intron-Distal3.139953.20659K562SND1
chr223280792032808018-intron-Distal5.024574.83371K562EFTUD2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RTCB is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RTCB is not available on ENCORE database.

  • 70 RBPs can affect the levels of RTCB.  








The Knockdown/knockout RNA-Seq assay for RTCB is not available on ENCORE database.





70 RBPs can affect the gene expression of RTCB :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RTCBENSG00000100220.71513.735902077.85720-0.457003.081202.04950HepG2CCAR1
RTCBENSG00000100220.7851.134501278.06880-0.586505.998905.02100HepG2CPSF6
RTCBENSG00000100220.71056.348901425.25990-0.432105.782404.79880HepG2DNAJC21
RTCBENSG00000100220.71141.794301412.56560-0.307004.284003.50570HepG2DNAJC2
RTCBENSG00000100220.71819.653202413.33600-0.407403.363602.71390HepG2EIF2S1
RTCBENSG00000100220.71836.184202667.01040-0.538504.839403.99780HepG2EIF4A3
RTCBENSG00000100220.71639.424602568.80220-0.647905.780104.62140HepG2EIF4G2
RTCBENSG00000100220.72685.873503626.67170-0.433309.007808.02690HepG2ETF1
RTCBENSG00000100220.72286.935601726.126200.405804.619303.54520HepG2EWSR1
RTCBENSG00000100220.71101.969001455.73000-0.401703.740902.83470HepG2FUBP3
RTCBENSG00000100220.71859.385802496.21750-0.424903.383902.65180HepG2G3BP1
RTCBENSG00000100220.71029.551601600.48860-0.636508.164807.02210HepG2HNRNPC
RTCBENSG00000100220.71701.752702168.42720-0.349605.539704.56770HepG2HNRNPF
RTCBENSG00000100220.7924.377001382.61370-0.580805.723904.85180HepG2HNRNPLL
RTCBENSG00000100220.71329.948801589.90980-0.257602.874702.25770HepG2HNRNPU
RTCBENSG00000100220.71790.917002135.27210-0.253702.340801.67280HepG2IGF2BP1
RTCBENSG00000100220.71882.588702537.34510-0.430603.721402.94830HepG2IGF2BP2
RTCBENSG00000100220.71671.711902552.14580-0.610405.090003.69250HepG2KHSRP
RTCBENSG00000100220.71933.704202691.87020-0.477204.430103.60240HepG2MAGOH
RTCBENSG00000100220.7991.955001410.07080-0.507402.830201.85150HepG2MTPAP
RTCBENSG00000100220.72250.873902770.69120-0.299801.943401.40760HepG2NCBP2
RTCBENSG00000100220.72084.919002694.92430-0.370303.665302.78660HepG2NOL12
RTCBENSG00000100220.71779.753402467.46360-0.471404.219703.33950HepG2PABPC4
RTCBENSG00000100220.72020.834402474.94500-0.292404.345603.61470HepG2PCBP1
RTCBENSG00000100220.72019.760702518.30580-0.318304.761604.08640HepG2PTBP1
RTCBENSG00000100220.72210.778602727.47960-0.303003.651802.94890HepG2QKI
RTCBENSG00000100220.72214.870601525.142500.538202.852902.13850HepG2RAVER1
RTCBENSG00000100220.72172.387003031.23480-0.480608.835108.02980HepG2RBM22
RTCBENSG00000100220.71977.785202475.32820-0.323703.303602.57230HepG2RBM25
RTCBENSG00000100220.72209.817002802.66850-0.342905.703604.97050HepG2NELFE
RTCBENSG00000100220.72124.317202610.23970-0.297204.506603.74490HepG2RECQL
RTCBENSG00000100220.71558.642701909.17520-0.292702.049001.35390HepG2RPS2
RTCBENSG00000100220.71434.718101885.45740-0.394102.287201.55830HepG2RPS3A
RTCBENSG00000100220.71180.817901415.90630-0.261903.061702.39000HepG2SAFB2
RTCBENSG00000100220.72322.131401792.256500.373602.320701.70310HepG2SF3A3
RTCBENSG00000100220.72167.924602621.95900-0.274303.945103.25960HepG2SF3B4
RTCBENSG00000100220.72000.642202467.19160-0.302402.317201.66600HepG2SLBP
RTCBENSG00000100220.73075.414402736.725900.168201.839301.43120HepG2SRSF1
RTCBENSG00000100220.71660.861202130.44160-0.359205.960505.00080HepG2SRSF7
RTCBENSG00000100220.71453.411102226.44500-0.6153016.3074015.16810HepG2SRSF9
RTCBENSG00000100220.72612.626002972.59310-0.186202.212501.74140HepG2SSB
RTCBENSG00000100220.7971.911601347.60150-0.471504.305703.42710HepG2TARDBP
RTCBENSG00000100220.71201.764801545.02990-0.362503.881803.31060HepG2TIAL1
RTCBENSG00000100220.71177.969401558.69600-0.404006.424105.62890HepG2TRIM56
RTCBENSG00000100220.72058.579001534.558000.423707.508606.60060HepG2UTP3
RTCBENSG00000100220.71060.040601429.19400-0.431107.311706.30580HepG2ZC3H8
RTCBENSG00000100220.71078.338501523.82090-0.498903.287401.86270HepG2ZRANB2
RTCBENSG00000100220.72313.661802037.637100.183202.005601.50740K562AGGF1
RTCBENSG00000100220.72681.884802224.688300.269503.216502.22700K562ATP5C1
RTCBENSG00000100220.71514.979701904.87590-0.330404.811603.52550K562BCLAF1
RTCBENSG00000100220.7582.81130872.43490-0.582003.446602.24180K562CCAR1
RTCBENSG00000100220.7972.405601326.70400-0.448203.050302.22830K562CPSF6
RTCBENSG00000100220.72708.092702251.401300.266403.625302.89190K562DDX47
RTCBENSG00000100220.72866.281002281.488800.329104.821503.61390K562DKC1
RTCBENSG00000100220.71938.796302442.58900-0.333204.684603.78030K562EIF2S2
RTCBENSG00000100220.71819.647601492.028800.286303.041902.20770K562EIF3G
RTCBENSG00000100220.72837.857703573.11010-0.332405.278904.00170K562EIF4G2
RTCBENSG00000100220.71009.543001349.77460-0.419002.888602.01460K562FMR1
RTCBENSG00000100220.7895.126501144.00570-0.353902.307401.47660K562FTO
RTCBENSG00000100220.72030.859301618.325000.327504.312903.58140K562FXR1
RTCBENSG00000100220.71286.021901660.02630-0.368302.095601.49200K562G3BP2
RTCBENSG00000100220.7856.082301452.35210-0.7626011.7462010.59100K562HNRNPC
RTCBENSG00000100220.7999.564801296.91210-0.375703.226501.98470K562HNRNPF
RTCBENSG00000100220.72627.447102031.326800.371102.994101.70830K562HNRNPK
RTCBENSG00000100220.71977.060101481.262500.416403.252102.23590K562HNRNPM
RTCBENSG00000100220.7763.990501081.49900-0.501403.166602.15950K562KRR1
RTCBENSG00000100220.71634.892501935.84840-0.243803.763002.90840K562NSUN2
RTCBENSG00000100220.72086.371701623.934800.361403.234302.42300K562PRPF8
RTCBENSG00000100220.73401.048504146.84580-0.286003.323502.14840K562PUM1
RTCBENSG00000100220.73113.133304062.91440-0.384106.769605.24940K562PUM2
RTCBENSG00000100220.72424.963202048.773200.243102.575001.99970K562RBM15
RTCBENSG00000100220.7955.016901188.01170-0.315002.556201.60740K562RCC2
RTCBENSG00000100220.71981.927801487.537800.413905.194004.06130K562SYNCRIP
RTCBENSG00000100220.7916.519901252.48070-0.450603.446202.39650K562TIAL1
RTCBENSG00000100220.72188.218101826.402500.260702.218001.45280K562WDR43
RTCBENSG00000100220.71257.805501694.42230-0.429902.702401.51360K562XRN2
RTCBENSG00000100220.72699.953603607.62560-0.418104.835303.64660K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RTCB is not available on ENCORE database.






No RBPs affect the alternative splcing events of RTCB on ENCORE database.





The Knockdown/knockout RNA-Seq assay for RTCB is not avaliable on ENCORE database.