| K562 | 
Caption: IP-WB analysis of K562 whole cell lysate using the RTCB specific antibody, A305-079A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RTCB-specific antibody, A305-079A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Comments: The buffer compositions are different between lanes 1 (lysis) and 3 (wash buffer), which may cause proteins to run slightly differently. See attached brochure Method: immunoprecipitation Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against RTCB in K562 whole cell lysate using RTCB specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RTCB. RTCB protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow. Releated Sample: BGKcLV35-63 Status: Released Lab: Graveley Lab |
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