CCNT1

General Information

Gene Name       : CCNT1
Gene ID             : ENSG00000129315.10
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: CCNT1)


Antibody Validation

 View validation images for all CCNT1 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CCNT1ENSG00000129315.1016.7315.9916.36ENCSR000AEF
HepG2CCNT1ENSG00000129315.1011.4212.0911.75ENCSR985KAT
K562CCNT1ENSG00000129315.1019.6122.4921.05ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CCNT1-BGKcLV26-K562ENCSR035MKJCCNT1K562ReleasedENCSR900GQL   CRISPRKD% = 74.6,72.5






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CCNT1ENCAB283VHIA303-496A1Released, ENCODE4BGKcLV26-65, Released, ENCODE4
CCNT1ENCAB973MTRA303-497A1Released, ENCODE4BGKcLV26-65, Released, ENCODE4
CCNT181464S1Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CCNT1K562CRISPRENCSR035MKJENCSR900GQLCCNT1-BGKcLV26-65,CCNT1-BGKcLV26-66NT-BGKcLV26-1,NT-BGKcLV26-274.6,72.5ReleasedENCODE4
CCNT1K562CRISPRBGKcLV44-91,BGKcLV44-92BGKcLV44-1,BGKcLV44-279.5,85.5NotSatisfiedENCORE2
CCNT1K562CRISPRBGKcLV44-93,BGKcLV44-94BGKcLV44-1,BGKcLV44-283.9,85.3NotSatisfiedENCORE2
CCNT1HepG2CRISPRBGHcLV27-47,BGHcLV27-48BGHcLV27-1,BGHcLV27-21.0,0.0NotSatisfiedENCODE4
CCNT1HepG2CRISPRBGHcLV45-9,BGHcLV45-10BGHcLV45-1,BGHcLV45-283.7,84.2148SequencingENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CCNT1Product_ID: A303-496A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CCNT1-human		 Bethyl_A303-496A_1_CCNT1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CCNT1 specific antibody, A303-496A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CCNT1-specific antibody, A303-496A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CCNT1-K562-CRISPR-A303-496A.png<br>Caption: Western blot following CRISPR against CCNT1 in K562 whole cell lysate using CCNT1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCNT1. CCNT1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
CCNT1Product_ID: A303-497A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CCNT1-human		 Bethyl_A303-497A_1_CCNT1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CCNT1 specific antibody, A303-497A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CCNT1-specific antibody, A303-497A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CCNT1-K562-CRISPR-A303-497A.png<br>Caption: Western blot following CRISPR against CCNT1 in K562 whole cell lysate using CCNT1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CCNT1. CCNT1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
CCNT1Product_ID: 81464S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: CCNT1-human		 CST_81464S_1_CCNT1.png<br>Caption: IP-WB analysis of 81464S whole cell lysate using the CCNT1 specific antibody, 81464S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CCNT1-specific antibody, 81464S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.








RNA Targets

  • eCLIP assay for CCNT1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • CCNT1 RNA is bound by 24 proteins.  







eCLIP assay for CCNT1 is not avaliable on ENCORE database.





24 proteins are bound to CCNT1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr124908503949085090-3'-UTR,intron-Distal3.832634.87260HepG2HNRNPM
chr124908503649085136-3'-UTR,intron-Distal5.1117512.87307K562EXOSC5
chr124908776749087871-3'-UTR,CDS,intron-Distal4.057623.67596K562GRWD1
chr124908688749086938-3'-UTR,CDS,intron-Distal4.144083.81597K562RBM15
chr124908294249082988-3'-UTR,intron-Distal3.818343.02850K562YBX3
chr124908734649087452-3'-UTR,CDS,intron-Distal3.150524.05663K562HNRNPL
chr124908613249086182-3'-UTR,intron-Distal3.584374.14074K562UPF1
chr124908227149082365-3'-UTR,intron-Distal3.742495.40624K562KHSRP
chr124908618249086255-3'-UTR,intron-Distal5.0583421.21593K562GTF2F1
chr124908540949085459-3'-UTR,intron-Distal3.320193.68481HepG2HNRNPM
chr124908618749086249-3'-UTR,intron-Distal3.208506.91711K562TIA1
chr124908578149085804-3'-UTR,intron-Distal3.167983.43829K562EXOSC5
chr124908494249084992-3'-UTR,intron-Distal4.319865.04680K562HNRNPM
chr124908782249087866-3'-UTR,CDS,intron-Distal3.497344.51666K562RBM15
chr124908804449088090-3'-UTR,CDS,intron-Distal4.012754.07825K562YBX3
chr124908619949086270-3'-UTR,intron-Distal4.017115.64899HepG2PCBP2
chr124908605849086173-3'-UTR,intron-Distal4.649489.34567HepG2FUBP3
chr124908629849086325-3'-UTR,intron-Distal3.006334.23390K562TIA1
chr124908713049087204-3'-UTR,CDS,intron-Distal4.563675.00478HepG2PCBP2
chr124908735149087442-3'-UTR,CDS,intron-Distal4.432433.98311K562IGF2BP1
chr124908414749084196-3'-UTR,intron-Distal4.118217.31419K562EXOSC5
chr124908469749084737-3'-UTR,intron-Distal3.125804.62871HepG2HNRNPM
chr124908399849084084-3'-UTR,intron-Distal4.053743.74511K562IGF2BP1
chr124908414049084199-3'-UTR,intron-Distal3.535513.68481HepG2HNRNPM
chr124908605349086140-3'-UTR,intron-Distal3.587619.80275K562FAM120A
chr124908529449085330-3'-UTR,intron-Distal4.931027.06230K562EXOSC5
chr124908619649086267-3'-UTR,intron-Distal3.523477.14301HepG2FAM120A
chr124908607849086121-3'-UTR,intron-Distal3.173663.08258K562TIA1
chr124908801349088146-3'-UTR,CDS,intron-Distal4.444765.13243HepG2GRWD1
chr124908619649086245-3'-UTR,intron-Distal4.9420514.46250K562DDX6
chr124908618049086254-3'-UTR,intron-Distal3.5519811.83905K562FAM120A
chr124908519549085221-3'-UTR,intron-Distal4.542215.12432K562EXOSC5
chr124911041449110476-5'-UTR,CDS,intron-Distal,intron-Proximal3.851886.84505K562ZNF622
chr124911039749110492-5'-UTR,CDS,intron-Distal,intron-Proximal4.215343.39325K562SRSF1
chr124908989549089967-CDS,intron-Distal,intron-Proximal3.465134.17944K562BUD13
chr124909192749091975-CDS,intron-Distal,intron-Proximal5.154437.19009HepG2U2AF2
chr124909354449093624-CDS,intron-Distal,intron-Proximal3.345603.94979HepG2BCLAF1
chr124910822449108268-CDS,intron-Distal,intron-Proximal3.931524.01763HepG2GRWD1
chr124911030349110349-CDS,intron-Distal4.467584.07072K562UCHL5
chr124909480549094852-CDS,intron-Distal4.351884.55944K562ZNF622
chr124909351849093606-CDS,intron-Distal,intron-Proximal4.9880512.15723HepG2PRPF8
chr124911036049110458-CDS,intron-Distal3.144955.54854HepG2GRWD1
chr124909481349094900-CDS,intron-Distal,intron-Proximal3.577174.57435K562SF3B4
chr124909960849099646-CDS,intron-Distal3.152333.02345K562ZNF622
chr124910027649100329-intron-Distal4.831498.24653K562KHSRP
chr124909775649097820-intron-Distal4.275063.90026K562KHSRP
chr124909125349091308-intron-Distal3.771776.98396HepG2HNRNPC
chr124909944949099505-intron-Distal,intron-Proximal4.281204.22101K562PRPF8
chr124909868749098771-intron-Distal4.107634.71638K562HNRNPL
chr124910021749100276-intron-Distal3.808596.48117K562KHSRP
chr124910810849108190-intron-Distal,intron-Proximal4.020945.99968K562PRPF8
chr124909782049097850-intron-Distal4.381265.02888K562KHSRP
chr124909945449099500-intron-Distal,intron-Proximal4.196975.31925K562U2AF2
chr124909182849091889-intron-Distal,intron-Proximal4.676915.82778K562PRPF8
chr124910810749108184-intron-Distal,intron-Proximal3.137215.34873K562KHSRP
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CCNT1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CCNT1 is not available on ENCORE database.

  • 65 RBPs can affect the levels of CCNT1.  








The Knockdown/knockout RNA-Seq assay for CCNT1 is not available on ENCORE database.





65 RBPs can affect the gene expression of CCNT1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CCNT1ENSG00000129315.51800.429001430.742500.331501.994401.53350HepG2AKAP1
CCNT1ENSG00000129315.5820.050201124.74780-0.455802.443701.71270HepG2CIRBP
CCNT1ENSG00000129315.5629.50180846.41920-0.427204.317003.45560HepG2DNAJC21
CCNT1ENSG00000129315.5718.67610838.99970-0.223301.901701.34240HepG2DNAJC2
CCNT1ENSG00000129315.5657.544101131.90790-0.783605.158504.36350HepG2EIF2S1
CCNT1ENSG00000129315.5926.595001251.03840-0.433102.038501.48730HepG2EIF4A3
CCNT1ENSG00000129315.52204.002801843.390600.257703.322302.67240HepG2ETF1
CCNT1ENSG00000129315.5964.555801261.80020-0.387502.010701.41750HepG2FXR1
CCNT1ENSG00000129315.5827.038401059.80190-0.357802.071601.51350HepG2GPKOW
CCNT1ENSG00000129315.5680.87900854.52870-0.327702.259701.67140HepG2HNRNPA1
CCNT1ENSG00000129315.5702.57390950.52340-0.436103.452602.68380HepG2HNRNPC
CCNT1ENSG00000129315.5618.38940944.23780-0.610609.492808.42160HepG2HNRNPU
CCNT1ENSG00000129315.5728.74130964.50450-0.404405.442304.44200HepG2ILF2
CCNT1ENSG00000129315.51133.498701528.01930-0.430902.752901.92510HepG2LARP4
CCNT1ENSG00000129315.5811.714401269.37570-0.645103.976403.23240HepG2MATR3
CCNT1ENSG00000129315.5979.892101243.82900-0.344103.595802.72680HepG2NOL12
CCNT1ENSG00000129315.51494.66560868.373500.783303.211602.25050HepG2PABPC1
CCNT1ENSG00000129315.5771.12180934.61100-0.277402.384901.59320HepG2PARN
CCNT1ENSG00000129315.51542.847501167.321400.402305.957705.12010HepG2PCBP1
CCNT1ENSG00000129315.5424.03150611.89840-0.529102.542701.36830HepG2PKM
CCNT1ENSG00000129315.5569.91280842.18150-0.563404.462003.54570HepG2PPIL4
CCNT1ENSG00000129315.5905.98040676.256300.421802.995502.15720HepG2PRPF8
CCNT1ENSG00000129315.5840.934801187.29980-0.497604.950504.18090HepG2PUF60
CCNT1ENSG00000129315.51005.127901259.43120-0.325403.099502.38930HepG2PUM2
CCNT1ENSG00000129315.51365.08880953.754400.517202.526401.85200HepG2RAVER1
CCNT1ENSG00000129315.51920.521001540.741100.317803.702403.12570HepG2RBM22
CCNT1ENSG00000129315.51113.791801321.90330-0.247102.210101.71580HepG2NELFE
CCNT1ENSG00000129315.5509.76340719.91620-0.498002.325501.75670HepG2RPS19
CCNT1ENSG00000129315.5880.44720668.969700.396202.465501.71960HepG2RRP9
CCNT1ENSG00000129315.5597.40730840.87480-0.493206.370305.45290HepG2SAFB2
CCNT1ENSG00000129315.52137.097801389.157600.621304.487503.61760HepG2SF3A3
CCNT1ENSG00000129315.5826.43350980.58410-0.246702.451401.84060HepG2SMN1
CCNT1ENSG00000129315.52352.550101740.224000.434909.338108.56740HepG2SRFBP1
CCNT1ENSG00000129315.51952.591601290.790100.5970013.4486012.57450HepG2SRSF1
CCNT1ENSG00000129315.51368.872301050.134300.382305.526704.79340HepG2SRSF9
CCNT1ENSG00000129315.51010.478501333.19440-0.399806.427805.54910HepG2SUCLG1
CCNT1ENSG00000129315.5532.16530925.70960-0.7987014.8504013.69190HepG2TRIM56
CCNT1ENSG00000129315.51342.210701031.740600.379402.882802.24220HepG2U2AF1
CCNT1ENSG00000129315.51050.810901231.96840-0.229502.265901.62340HepG2UPF1
CCNT1ENSG00000129315.51386.836301189.420100.221402.028901.49950HepG2UTP3
CCNT1ENSG00000129315.5587.63820858.93470-0.547603.787302.94800HepG2XRN2
CCNT1ENSG00000129315.51152.434201354.93220-0.233502.712202.16880HepG2ZNF622
CCNT1ENSG00000129315.52052.067701523.483800.429602.616301.47400K562AARS
CCNT1ENSG00000129315.51586.746602088.76470-0.396606.617705.79480K562AGGF1
CCNT1ENSG00000129315.57295.949903268.223201.1585031.7109029.91990K562AQR
CCNT1ENSG00000129315.51542.484201864.60360-0.273603.337002.28000K562BCLAF1
CCNT1ENSG00000129315.51945.503702496.25740-0.359604.263502.75960K562CCDC124
CCNT1ENSG00000129315.51879.700602400.39650-0.352803.202101.80970K562CIRBP
CCNT1ENSG00000129315.52514.417103061.77840-0.284103.917102.59230K562CPEB4
CCNT1ENSG00000129315.51689.177202004.30250-0.246802.096001.40460K562CPSF6
CCNT1ENSG00000129315.51694.897202272.98020-0.423406.717905.66940K562EIF2S2
CCNT1ENSG00000129315.52058.135701676.470800.295802.360801.60090K562EIF4A3
CCNT1ENSG00000129315.51711.082702502.07520-0.548206.923005.52810K562FIP1L1
CCNT1ENSG00000129315.52015.510001743.455800.209101.989301.45970K562FXR1
CCNT1ENSG00000129315.52788.637201830.916100.606905.184104.14730K562G3BP2
CCNT1ENSG00000129315.52357.225203029.16880-0.361803.896703.00380K562GRSF1
CCNT1ENSG00000129315.51137.771701488.96480-0.388102.360001.32010K562HNRNPF
CCNT1ENSG00000129315.52372.996902863.23570-0.270902.425301.44370K562HNRNPL
CCNT1ENSG00000129315.51529.396502057.97930-0.428303.397402.35770K562HNRNPM
CCNT1ENSG00000129315.52350.158703392.30140-0.529506.796605.55850K562HSPD1
CCNT1ENSG00000129315.52252.088902549.65220-0.179002.601701.87490K562NSUN2
CCNT1ENSG00000129315.52904.943602094.366200.471905.752604.33180K562PCBP2
CCNT1ENSG00000129315.52005.395302840.33110-0.502205.757804.50080K562PPIG
CCNT1ENSG00000129315.52197.763701679.828000.387603.558502.70680K562PRPF8
CCNT1ENSG00000129315.51102.681801495.20920-0.439302.487601.63550K562PSIP1
CCNT1ENSG00000129315.53462.509804375.70610-0.337705.070003.75310K562PUM2
CCNT1ENSG00000129315.52334.151701904.646500.293303.088202.18270K562SF3B4
CCNT1ENSG00000129315.51814.392602188.63700-0.270502.456001.69030K562TIA1
CCNT1ENSG00000129315.51181.118801527.40870-0.370902.362901.49990K562TRIM56
CCNT1ENSG00000129315.53084.656601775.670100.7966010.547408.20380K562U2AF1
CCNT1ENSG00000129315.52767.038101833.580600.5936010.868009.26630K562U2AF2
CCNT1ENSG00000129315.52116.209502703.45030-0.353306.627305.56950K562UPF1
CCNT1ENSG00000129315.51969.178602405.51870-0.288804.638003.56380K562WDR43
CCNT1ENSG00000129315.51331.802601752.78440-0.396303.071801.77680K562XRN2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CCNT1 is not available on ENCORE database.





13 RBPs can affect the splicing of CCNT1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.137000.005460.08127excludedK562SRSF1
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.250000.000240.00637excludedK562SF3B4
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.379000.000000.00004excludedK562PRPF8
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.436000.000000.00000excludedK562XRCC6
SECCNT1ENSG00000129315.5chr12-490895414908961249086815490882194908978149089945-0.065000.005130.04751excludedK562AQR
SECCNT1ENSG00000129315.5chr12-490995504909967949082246490882194910822549108307-0.405000.000140.00326excludedK562PUS1
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.355000.000140.00638excludedHepG2SF3A3
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.319000.001150.07450excludedHepG2TIAL1
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.208000.000660.01422excludedK562U2AF2
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.474000.000000.00048excludedHepG2HNRNPU
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091966-0.306000.000100.03033excludedK562SERBP1
SECCNT1ENSG00000129315.5chr12-490897814908994549089541490896124909192049091962-0.321000.000000.00001excludedK562PTBP1
A3SSCCNT1ENSG00000129315.5chr12-4908954149089681490895414908961249091920490919660.459000.000810.00578longerK562AQR
A3SSCCNT1ENSG00000129315.5chr12-4909479549094884490947954909485649099550490997420.322000.000000.00000longerK562AQR
MXECCNT1ENSG00000129315.5chr12-4909479549094856490995504909967949082246490882194910822549108307-0.292000.000020.000872includedK562PUS1
MXECCNT1ENSG00000129315.5chr12-4909479549094856490995504909967949086815490882194910822549108307-0.095000.011530.071082includedK562AQR
MXECCNT1ENSG00000129315.5chr12-4908978149089945490919204909196649082246490882194909356049093623-0.100000.003780.028412includedK562AQR
MXECCNT1ENSG00000129315.5chr12-49089781490899454909192049091966490895414908961249094795490948560.247000.002270.030621includedK562U2AF2
MXECCNT1ENSG00000129315.5chr12-4909192049091966490935604909362349089541490896124909479549094856-0.140000.000940.066012includedK562AARS

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CCNT1 is not avaliable on ENCORE database.