RPS28

General Information

Gene Name : RPS28
Gene ID : ENSG00000233927.4
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: RPS28)

Not Available

Antibody Validation

Bethyl Labs: A305-095A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	RPS28	ENSG00000233927.4	46.57	442.97	244.77	ENCSR000AEF
HepG2	RPS28	ENSG00000233927.4	1314.19	223.56	768.88	ENCSR985KAT
K562	RPS28	ENSG00000233927.4	763.52	582.50	673.01	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	RPS28-BGKcLV33-K562		RPS28	K562	Released			CRISPR	KD% = 12.2,18.2
knockdown followed by RNA-seq	RPS28-BGKcLV33-K562	ENCSR928XFZ	RPS28	K562	Released		ENCSR271ZAB	CRISPR	KD% = 0.0,0.0

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
RPS28	ENCAB557DQG	A305-095A	1					BGKcLV33-13, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
RPS28	K562	CRISPR			BGKcLV33-5,BGKcLV33-6	BGKcLV33-1,BGKcLV33-2	12.2,18.2	Released	ENCODE4
RPS28	K562	CRISPR	ENCSR928XFZ	ENCSR271ZAB	RPS28-BGKcLV33-13,RPS28-BGKcLV33-14	NT-BGKcLV33-1,NT-BGKcLV33-2	0.0,0.0	Released	ENCODE4
RPS28	HepG2	CRISPR			BGHcLV31-7,BGHcLV31-8	BGHcLV31-1,BGHcLV31-2	0.0,0.0	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
RPS28	Product_ID: A305-095A Lot_ID: 1 Source: Bethyl Labs Target Name: RPS28-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for RPS28 is not avaliable on ENCORE database.

Proteins bound to RNA

RPS28 RNA is bound by 18 proteins.

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eCLIP assay for RPS28 is not avaliable on ENCORE database.

18 proteins are bound to RPS28 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr19	8386555	8386563	+	CDS	3.31312	400.00000	K562	YBX3
chr19	8386539	8386555	+	CDS,intron-Proximal	3.32714	400.00000	K562	YBX3
chr19	8386450	8386453	+	CDS	3.49113	400.00000	K562	YBX3
chr19	8386435	8386443	+	CDS	3.54057	400.00000	K562	YBX3
chr19	8386443	8386450	+	CDS	3.46256	400.00000	K562	YBX3
chr19	8386557	8386588	+	CDS,intron-Proximal	3.98846	400.00000	K562	PABPC4
chr19	8386836	8386854	+	CDS,intron-Proximal	4.29916	67.72941	K562	PABPC4
chr19	8386557	8386583	+	CDS	3.55877	61.38149	K562	DDX3X
chr19	8386947	8386975	+	5'-UTR,CDS	3.28485	49.94950	K562	PABPC4
chr19	8386583	8386588	+	CDS,intron-Proximal	3.92588	49.53567	K562	DDX3X
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 73 entries

eCLIP assay for RPS28 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for RPS28 is not available on ENCORE database.
106 RBPs can affect the levels of RPS28.

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The Knockdown/knockout RNA-Seq assay for RPS28 is not available on ENCORE database.

106 RBPs can affect the gene expression of RPS28 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
RPS28	ENSG00000233927.4	2209.71070	910.94710	1.27830	68.67630	66.04190	HepG2	HNRNPL
RPS28	ENSG00000233927.4	2146.75520	1004.91670	1.09500	53.14950	51.23760	HepG2	PUM1
RPS28	ENSG00000233927.4	1003.05860	333.17920	1.58990	50.68520	48.67250	HepG2	TRIM56
RPS28	ENSG00000233927.4	2010.07880	907.72030	1.14680	42.44630	40.39960	HepG2	SLBP
RPS28	ENSG00000233927.4	1080.07660	302.63970	1.83540	37.64040	35.68900	HepG2	SAFB2
RPS28	ENSG00000233927.4	1775.30170	1000.04840	0.82790	27.73330	25.85160	HepG2	DAZAP1
RPS28	ENSG00000233927.4	642.97210	301.98490	1.09020	22.12890	20.44560	HepG2	DNAJC2
RPS28	ENSG00000233927.4	708.83880	347.13900	1.02980	20.20690	18.43920	HepG2	ILF2
RPS28	ENSG00000233927.4	9057.63070	5088.33660	0.83180	19.09320	17.72510	K562	AGGF1
RPS28	ENSG00000233927.4	4927.13730	1979.88760	1.31520	19.76970	16.17960	K562	TRA2A
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 118 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for RPS28 is not available on ENCORE database.

5 RBPs can affect the splicing of RPS28 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
MXE	RPS28	ENSG00000233927.4	chr19	+	8386539	8386587	8386836	8386975	8386383	8386453	8387155	8388224	0.07300	0.00002	0.00331	1included	K562	TRA2A
MXE	RPS28	ENSG00000233927.4	chr19	+	8386539	8386587	8386836	8386975	8386383	8386453	8387155	8388224	0.06600	0.00018	0.00672	1included	K562	SUPV3L1
MXE	RPS28	ENSG00000233927.4	chr19	+	8386539	8386587	8386836	8386975	8386383	8386453	8387155	8388224	0.08500	0.00116	0.07820	1included	K562	CPSF6
MXE	RPS28	ENSG00000233927.4	chr19	+	8386539	8386587	8386836	8386975	8386383	8386453	8387155	8388224	0.07800	0.00192	0.03218	1included	K562	SF3B4
RI	RPS28	ENSG00000233927.4	chr19	+	8386525	8386703			8386525	8386587	8386667	8386703	-0.11500	0.01058	0.05650	excluded	K562	AQR

Showing 1 to 5 of 5 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

RPS28

General Information

Functions

Localization

Domains (AlphaFold: RPS28)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for RPS28 is not avaliable on ENCORE database.

18 proteins are bound to RPS28 RNA:

eCLIP assay for RPS28 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for RPS28 is not available on ENCORE database.

106 RBPs can affect the gene expression of RPS28 :

The Knockdown/knockout RNA-Seq assay for RPS28 is not available on ENCORE database.

5 RBPs can affect the splicing of RPS28 :

The Knockdown/knockout RNA-Seq assay for RPS28 is not avaliable on ENCORE database.