NCBP1

General Information

Gene Name : NCBP1
Gene ID : ENSG00000136937.12
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: NCBP1)

Not Available

Antibody Validation

View validation images for all NCBP1 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	NCBP1	ENSG00000136937.12	27.75	25.85	26.80	ENCSR000AEF
HepG2	NCBP1	ENSG00000136937.12	21.28	30.00	25.64	ENCSR985KAT
K562	NCBP1	ENSG00000136937.12	40.21	43.48	41.84	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	NCBP1-BGKcLV11-K562	ENCSR090SBZ	NCBP1	K562	Released		ENCSR292PXV	CRISPR	KD% = 64.6,66.5
knockdown followed by RNA-seq	NCBP1-BGHcLV10-HepG2	ENCSR324GSI	NCBP1	HepG2	Released		ENCSR775ZWO	CRISPR	KD% = 60.2,54.4

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
NCBP1	ENCAB987FLC	RN103PW	1		Released, ENCODE3		BGHcLV10-25, Released, ENCODE4	BGKcLV11-61, Released, ENCODE4
NCBP1		24964S	1		Released, ENCODE3	Submitted, ENCORE2	BGHcLV10-25, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
NCBP1	K562	CRISPR			BGKcLV11-59,BGKcLV11-60	BGKcLV11-1,BGKcLV11-2	56.7,62.3	NotSatisfied	ENCODE4
NCBP1	K562	CRISPR	ENCSR090SBZ	ENCSR292PXV	NCBP1-BGKcLV11-61,NCBP1-BGKcLV11-62	NT-BGKcLV11-1,NT-BGKcLV11-2	64.6,66.5	Released	ENCODE4
NCBP1	K562	CRISPR			BGKcLV11-63,BGKcLV11-64	BGKcLV11-1,BGKcLV11-2	34.4,56.0	NotSatisfied	ENCODE4
NCBP1	HepG2	CRISPR	ENCSR324GSI	ENCSR775ZWO	NCBP1-BGHcLV10-25,NCBP1-BGHcLV10-26	NT-BGHcLV10-1,NT-BGHcLV10-2	60.2,54.4	Released	ENCODE4
NCBP1	HepG2	CRISPR			BGHcLV10-27,BGHcLV10-28	BGHcLV10-1,BGHcLV10-2	48.1,53.8	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
NCBP1	Product_ID: RN103PW Lot_ID: 1 Source: MBLI Target Name: NCBP1-human
NCBP1	Product_ID: 24964S Lot_ID: 1 Source: Cell Signaling Technology Target Name: NCBP1-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for NCBP1 is not avaliable on ENCORE database.

Proteins bound to RNA

NCBP1 RNA is bound by 20 proteins.

CanvasJS.com

eCLIP assay for NCBP1 is not avaliable on ENCORE database.

20 proteins are bound to NCBP1 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr9	100431881	100431923	+	CDS	6.08098	17.94432	K562	YBX3
chr9	100435532	100435671	+	3'-UTR,intron-Distal	4.07172	10.92054	K562	UPF1
chr9	100396076	100396198	+	5'-UTR,CDS,intron-Proximal	4.69567	10.17596	K562	DDX3X
chr9	100433539	100433611	+	3'-UTR	4.28494	9.94910	K562	KHSRP
chr9	100403969	100403993	+	intron-Proximal	5.56086	8.83608	K562	AQR
chr9	100403996	100404147	+	intron-Distal,intron-Proximal	4.43098	8.53255	K562	PRPF8
chr9	100416123	100416145	+	CDS	3.58270	8.28018	K562	YBX3
chr9	100403943	100403969	+	CDS,intron-Proximal	5.46454	7.63570	K562	AQR
chr9	100403842	100403938	+	CDS,intron-Proximal	3.63745	7.21479	K562	UCHL5
chr9	100431953	100432028	+	CDS,intron-Proximal	3.98246	7.02084	HepG2	PRPF8
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 41 entries

eCLIP assay for NCBP1 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for NCBP1 is not available on ENCORE database.
126 RBPs can affect the levels of NCBP1.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for NCBP1 is not available on ENCORE database.

126 RBPs can affect the gene expression of NCBP1 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
NCBP1	ENSG00000136937.8	1006.22200	2280.77090	-1.18060	30.58620	28.67680	K562	HNRNPC
NCBP1	ENSG00000136937.8	578.82020	1280.44490	-1.14550	20.75920	19.04510	HepG2	HNRNPC
NCBP1	ENSG00000136937.8	897.58110	1508.54680	-0.74900	20.32760	18.66410	HepG2	HNRNPL
NCBP1	ENSG00000136937.8	714.99270	1410.04290	-0.97970	19.56280	18.01870	HepG2	SRSF7
NCBP1	ENSG00000136937.8	695.18270	1460.67050	-1.07120	17.89500	16.32490	HepG2	CPSF6
NCBP1	ENSG00000136937.8	1801.14270	1104.33650	0.70560	16.99370	16.03270	HepG2	SRSF1
NCBP1	ENSG00000136937.8	756.20570	1247.00900	-0.72160	15.38190	14.20220	HepG2	TRIM56
NCBP1	ENSG00000136937.8	924.22720	1540.28750	-0.73690	15.34610	14.07460	HepG2	CELF1
NCBP1	ENSG00000136937.8	683.94540	1132.77400	-0.72790	15.40170	14.06700	HepG2	SAFB2
NCBP1	ENSG00000136937.8	811.93610	1580.19430	-0.96070	14.38790	13.10760	HepG2	HNRNPLL
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 155 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for NCBP1 is not available on ENCORE database.

12 RBPs can affect the splicing of NCBP1 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	NCBP1	ENSG00000136937.8	chr9	+	100413591	100413655			100412784	100412882	100416079	100416190	-0.24600	0.00000	excluded	HepG2	U2AF1
SE	NCBP1	ENSG00000136937.8	chr9	+	100413591	100413655			100412784	100412882	100416079	100416190	-0.29200	0.00000	excluded	HepG2	U2AF2
SE	NCBP1	ENSG00000136937.8	chr9	+	100407892	100408014			100407367	100407506	100409773	100409843	-0.08300	0.00000	excluded	K562	AQR
SE	NCBP1	ENSG00000136937.8	chr9	+	100413591	100413655			100412784	100412882	100416079	100416190	-0.11500	0.00000	excluded	K562	AQR
SE	NCBP1	ENSG00000136937.8	chr9	+	100413591	100413655			100412784	100412882	100416079	100416190	-0.11100	0.00005	excluded	K562	PUS1
SE	NCBP1	ENSG00000136937.8	chr9	+	100413591	100413655			100412784	100412882	100416079	100416190	-0.25100	0.00000	excluded	K562	U2AF1
SE	NCBP1	ENSG00000136937.8	chr9	+	100413591	100413655			100412784	100412882	100416079	100416190	-0.18700	0.00001	excluded	K562	U2AF2
SE	NCBP1	ENSG00000136937.8	chr9	+	100413591	100413655			100412784	100412882	100416079	100416190	-0.09700	0.00022	excluded	K562	RBM15
MXE	NCBP1	ENSG00000136937.8	chr9	+	100409773	100409807	100409807	100409843	100407892	100408014	100410289	100410505	-0.14300	0.00000	2included	K562	DROSHA
MXE	NCBP1	ENSG00000136937.8	chr9	+	100407398	100407506	100407892	100408014	100405485	100405642	100409773	100409843	0.20200	0.00001	1included	HepG2	U2AF2

Showing 1 to 10 of 18 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

NCBP1

General Information

Functions

Localization

Domains (AlphaFold: NCBP1)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for NCBP1 is not avaliable on ENCORE database.

20 proteins are bound to NCBP1 RNA:

eCLIP assay for NCBP1 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for NCBP1 is not available on ENCORE database.

126 RBPs can affect the gene expression of NCBP1 :

The Knockdown/knockout RNA-Seq assay for NCBP1 is not available on ENCORE database.

12 RBPs can affect the splicing of NCBP1 :

The Knockdown/knockout RNA-Seq assay for NCBP1 is not avaliable on ENCORE database.