AFF4

General Information

Gene Name : AFF4
Gene ID : ENSG00000072364.12
Essential Gene : No

Functions

Other

Localization

Not Available

Domains (AlphaFold: AFF4)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

View validation images for all AFF4 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	AFF4	ENSG00000072364.12	10.97	11.55	11.26	ENCSR000AEF
HepG2	AFF4	ENSG00000072364.12	9.72	15.40	12.56	ENCSR985KAT
K562	AFF4	ENSG00000072364.12	12.29	13.16	12.72	ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
AFF4	ENCAB106ONT	A302-538A	2			Released, ENCODE4
AFF4	ENCAB734KFK	A302-539A	1			Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
AFF4	K562	CRISPR			BGKcLV26-13,BGKcLV26-14	BGKcLV26-1,BGKcLV26-2	25.7,11.3	NotSatisfied	ENCODE4
AFF4	HepG2	CRISPR			BGHcLV25-17,BGHcLV25-18	BGHcLV25-1,BGHcLV25-2	no clear band on the position,	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
AFF4	Product_ID: A302-538A Lot_ID: 2 Source: Bethyl Labs Target Name: AFF4-human
AFF4	Product_ID: A302-539A Lot_ID: 1 Source: Bethyl Labs Target Name: AFF4-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for AFF4 is not avaliable on ENCORE database.

Proteins bound to RNA

AFF4 RNA is bound by 39 proteins.

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eCLIP assay for AFF4 is not avaliable on ENCORE database.

39 proteins are bound to AFF4 RNA:

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Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr5	132211851	132211913	-	3'-UTR,intron-Distal	4.37406	23.18737	K562	FAM120A
chr5	132227994	132228097	-	CDS,intron-Distal,intron-Proximal	4.88492	19.71265	K562	ZNF622
chr5	132211114	132211184	-	3'-UTR,intron-Distal	4.75488	18.52095	K562	PUM2
chr5	132227849	132227953	-	CDS,intron-Distal,intron-Proximal	3.90093	15.86208	K562	ZNF622
chr5	132227802	132227905	-	CDS,intron-Distal,intron-Proximal	4.38095	15.77157	HepG2	PRPF8
chr5	132234794	132234834	-	CDS,intron-Distal,intron-Proximal	5.17116	15.46734	K562	ZNF622
chr5	132227766	132227822	-	3'-UTR,CDS,intron-Distal,intron-Proximal	6.57238	14.81397	K562	AQR
chr5	132227989	132228095	-	CDS,intron-Distal	3.24705	14.47671	HepG2	BCLAF1
chr5	132248638	132248811	-	intron-Distal	4.18660	14.24414	K562	KHSRP
chr5	132228722	132228812	-	CDS,intron-Distal,intron-Proximal	5.99678	13.58086	HepG2	BCLAF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 149 entries

eCLIP assay for AFF4 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for AFF4 is not available on ENCORE database.
116 RBPs can affect the levels of AFF4.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for AFF4 is not available on ENCORE database.

116 RBPs can affect the gene expression of AFF4 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
AFF4	ENSG00000072364.8	8698.68520	4249.14830	1.03350	74.03740	72.35310	HepG2	SRFBP1
AFF4	ENSG00000072364.8	4895.76590	2545.76070	0.94330	48.80990	47.05930	HepG2	SRSF9
AFF4	ENSG00000072364.8	1490.87050	2853.28880	-0.93650	36.88300	35.12110	HepG2	TRIM56
AFF4	ENSG00000072364.8	1491.53770	2828.04600	-0.92300	24.93070	23.71420	HepG2	TIAL1
AFF4	ENSG00000072364.8	936.14080	2227.24250	-1.25050	23.54380	21.53160	HepG2	XRN2
AFF4	ENSG00000072364.8	8096.51820	4270.92170	0.92270	22.74950	20.32910	K562	PUF60
AFF4	ENSG00000072364.8	3704.56190	2066.08720	0.84230	21.66430	19.39000	K562	U2AF2
AFF4	ENSG00000072364.8	1551.25360	3417.24630	-1.13940	20.61870	19.04220	HepG2	GPKOW
AFF4	ENSG00000072364.8	1758.43790	2585.89830	-0.55640	17.10590	15.61770	HepG2	DNAJC2
AFF4	ENSG00000072364.8	4365.63040	2997.90400	0.54210	15.43110	14.20550	HepG2	SF3B4
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 140 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for AFF4 is not available on ENCORE database.

9 RBPs can affect the splicing of AFF4 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	AFF4	ENSG00000072364.8	chr5	-	132224770	132224865			132223788	132223852	132227855	132228096	-0.19600	0.00000	0.00000	excluded	HepG2	U2AF1
SE	AFF4	ENSG00000072364.8	chr5	-	132224770	132224865			132223788	132223852	132227855	132228096	-0.08500	0.00000	0.00000	excluded	HepG2	U2AF2
SE	AFF4	ENSG00000072364.8	chr5	-	132224770	132224865			132223788	132223852	132227855	132228096	-0.30600	0.00000	0.00000	excluded	K562	AQR
SE	AFF4	ENSG00000072364.8	chr5	-	132273340	132273448			132272758	132272885	132298950	132299326	0.11600	0.00000	0.00027	included	HepG2	LARP4
SE	AFF4	ENSG00000072364.8	chr5	-	132224770	132224865			132223788	132223852	132227855	132228096	-0.22800	0.00000	0.00001	excluded	K562	U2AF2
SE	AFF4	ENSG00000072364.8	chr5	-	132224770	132224865			132223788	132223852	132227855	132228096	-0.12100	0.00001	0.00022	excluded	K562	PUS1
SE	AFF4	ENSG00000072364.8	chr5	-	132233921	132234084			132232502	132232932	132234795	132234833	-0.16500	0.00003	0.00087	excluded	K562	PUS1
SE	AFF4	ENSG00000072364.8	chr5	-	132224770	132224865			132223788	132223852	132227855	132228096	-0.15100	0.00013	0.00405	excluded	K562	U2AF1
SE	AFF4	ENSG00000072364.8	chr5	-	132262812	132262899			132240059	132240096	132267869	132267914	0.09800	0.00017	0.01237	included	HepG2	SFPQ
MXE	AFF4	ENSG00000072364.8	chr5	-	132228721	132228810	132232014	132232932	132227508	132228096	132233921	132234084	0.20300	0.00051	0.00508	1included	K562	AQR

Showing 1 to 10 of 18 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

AFF4

General Information

Functions

Localization

Domains (AlphaFold: AFF4)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for AFF4 is not avaliable on ENCORE database.

39 proteins are bound to AFF4 RNA:

eCLIP assay for AFF4 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for AFF4 is not available on ENCORE database.

116 RBPs can affect the gene expression of AFF4 :

The Knockdown/knockout RNA-Seq assay for AFF4 is not available on ENCORE database.

9 RBPs can affect the splicing of AFF4 :

The Knockdown/knockout RNA-Seq assay for AFF4 is not avaliable on ENCORE database.