GPKOW

General Information

Gene Name : GPKOW
Gene ID : ENSG00000068394.10
Essential Gene : Yes

Functions

Splicing regulation
Spliceosome

Localization(RBPImage: GPKOW)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Speckles
Nuclear release mitosis

Domains (AlphaFold: GPKOW)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

GeneTex: GTX117716

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	GPKOW	ENSG00000068394.10	8.79	13.99	11.39	ENCSR000AEF
HepG2	GPKOW	ENSG00000068394.10	17.45	13.46	15.46	ENCSR985KAT
K562	GPKOW	ENSG00000068394.10	22.63	21.13	21.88	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 450	ENCSR647CLF	GPKOW	K562	Released	ENCAB445RKH	ENCSR393STP
knockdown followed by RNA-seq	GPKOW-BGKLV36-K562	ENCSR967QNT	GPKOW	K562	Released		ENCSR424QCW	shRNA	KD% = 50.0,57.0
knockdown followed by RNA-seq	GPKOW-BGHLV35-HepG2	ENCSR968YWY	GPKOW	HepG2	Released		ENCSR264TUE	shRNA	KD% = 62.3,67.3

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
GPKOW	450	K562	ENCSR647CLF	ENCSR393STP	ENCAB445RKH	GTX117716	40289	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
GPKOW	ENCAB445RKH	GTX117716	40289			Released, ENCODE3	BGHLV35-21, Released, ENCODE3	BGKLV36-27, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
GPKOW	K562	shRNA	ENCSR967QNT	ENCSR424QCW	GPKOW_BGKLV36-27,GPKOW_BGKLV36-28	NT_BGKLV36-1,NT_BGKLV36-2	50.0,57.0	Released	ENCODE3
GPKOW	HepG2	shRNA	ENCSR968YWY	ENCSR264TUE	GPKOW_BGHLV35-21,GPKOW_BGHLV35-22	NT_BGHLV35-1,NT_BGHLV35-2	62.3,67.3	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

GPKOW: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Speckles

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
GPKOW	Product_ID: GTX117716 Lot_ID: 40289 Source: GeneTex Target Name: GPKOW-human

RNA Targets

GPKOW protein binds to 1,128 RNA regions.

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Proteins bound to RNA

GPKOW RNA is bound by 5 proteins.

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GPKOW protein has 1,128 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
GPKOW	chr1	145441170	145441220	+	4.20310	400.00000	K562	TXNIP	3'-UTR,CDS,intron-Proximal
GPKOW	chr6	43745894	43745982	+	3.59820	400.00000	K562	VEGFA	intron-Distal
GPKOW	chr11	61732327	61732378	-	3.73452	42.61177	K562	FTH1	3'-UTR,CDS,intron-Distal,intron-Proximal
GPKOW	chr11	93466232	93466335	-	4.42623	27.99170	K562	TAF1D	3'-UTR,intron-Distal,intron-Proximal
GPKOW	chr1	145439591	145439628	+	3.20542	26.76718	K562	TXNIP	CDS,intron-Distal,intron-Proximal
GPKOW	chr4	166261533	166261564	+	4.11911	21.59709	K562	MSMO1	3'-UTR,intron-Proximal
GPKOW	chr1	150198945	150199072	-	3.47027	20.36579	K562	ANP32E	3'-UTR,CDS,intron-Distal,intron-Proximal
GPKOW	chr1	145440150	145440153	+	4.49205	18.92122	K562	TXNIP	intron-Distal,intron-Proximal
GPKOW	chr17	25635609	25635665	+	3.69310	18.13111	K562	WSB1	3'-UTR,intron-Distal
GPKOW	chr1	223957582	223957665	+	4.03304	17.14795	K562	CAPN2	CDS,intron-Proximal

Showing 1 to 10 of 100 entries

5 proteins are bound to GPKOW RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chrX	48973241	48973379	-	intron-Distal,intron-Proximal	6.97668	19.59836	K562	EFTUD2
chrX	48979813	48979898	-	CDS,intron-Distal	6.65727	17.53326	K562	AQR
chrX	48979081	48979177	-	CDS,intron-Distal,intron-Proximal	4.70760	12.35151	K562	AQR
chrX	48970455	48970504	-	3'-UTR,intron-Distal	5.27289	8.09274	K562	LIN28B
chrX	48970431	48970455	-	3'-UTR,intron-Distal	4.74649	5.20653	K562	LIN28B
chrX	48973897	48973952	-	CDS,intron-Distal,intron-Proximal	4.67206	4.86944	K562	AQR
chrX	48970448	48970556	-	3'-UTR,intron-Distal	4.00349	3.45275	HepG2	LARP4
chrX	48974082	48974109	-	CDS,intron-Distal	3.19874	3.24816	K562	YBX3
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 8 of 8 entries

No autoregularoty interactions between protein GPKOW and its RNA.

Diffrential Expression

12,245 differentially expressed genes after Knockdown/Knockout GPKOW protein.

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39 RBPs can affect the levels of GPKOW.

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12,245 differentially expressed genes after Knockdown/Knockout GPKOW protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
CRLF1	ENSG00000006016.6	515.74310	3080.52340	-2.57850	57.32850	54.87300	HepG2	GPKOW
ZFX	ENSG00000005889.11	2587.35770	659.77170	1.97130	51.67590	49.33880	HepG2	GPKOW
IL32	ENSG00000008517.12	8630.76480	2569.23070	1.74810	51.24220	48.91300	HepG2	GPKOW
ACSM3	ENSG00000005187.7	4414.09130	547.97240	3.00980	49.42130	47.13310	HepG2	GPKOW
MLXIPL	ENSG00000009950.11	13182.49640	4425.77770	1.57450	43.15710	41.01560	HepG2	GPKOW
ASB4	ENSG00000005981.8	5.74060	283.41090	-5.62560	39.51910	37.43910	HepG2	GPKOW
CYP51A1	ENSG00000001630.11	11396.75040	4539.94150	1.32780	31.20950	29.34150	HepG2	GPKOW
JHDM1D	ENSG00000006459.6	428.48120	1309.86630	-1.61210	29.60190	27.77020	HepG2	GPKOW
MASP2	ENSG00000009724.12	798.06220	235.40190	1.76130	27.26780	25.49430	HepG2	GPKOW
RHOBTB2	ENSG00000008853.12	1139.21360	448.97090	1.34320	20.53940	18.96700	HepG2	GPKOW
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

39 RBPs can affect the gene expression of GPKOW :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
GPKOW	ENSG00000068394.9	101.19850	473.51430	-2.22620	29.06150	27.25160	HepG2	GPKOW
GPKOW	ENSG00000068394.9	202.09630	591.30930	-1.54890	23.70100	21.21690	K562	GPKOW
GPKOW	ENSG00000068394.9	108.60730	280.04180	-1.36650	9.71720	8.23350	K562	G3BP2
GPKOW	ENSG00000068394.9	226.38090	447.26500	-0.98240	8.95350	7.75280	HepG2	SF3B1
GPKOW	ENSG00000068394.9	241.74540	429.32490	-0.82860	7.91180	7.04240	HepG2	TAF15
GPKOW	ENSG00000068394.9	278.40440	457.54610	-0.71670	6.56010	5.71210	HepG2	SF3B4
GPKOW	ENSG00000068394.9	572.54540	392.54710	0.54440	6.13280	5.40200	HepG2	ZNF622
GPKOW	ENSG00000068394.9	378.87540	558.65310	-0.56020	5.77040	4.95500	HepG2	ETF1
GPKOW	ENSG00000068394.9	268.23960	440.50450	-0.71560	5.12880	4.18590	HepG2	TARDBP
GPKOW	ENSG00000068394.9	215.32520	333.93970	-0.63310	4.91710	3.91560	HepG2	PRPF4
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 44 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

285 alternative splicing events after Knockdown/Knockout GPKOW protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	SNRNP70	ENSG00000104852.10	chr19	+	49605370	49606844	49604646	49604728	49607890	49607992	0.17400	0.00000	included	K562	GPKOW
SE	STXBP2	ENSG00000076944.10	chr19	+	7712239	7712397	7712047	7712133	7712610	7712759	0.13100	0.00177	included	K562	GPKOW
SE	NBEAL2	ENSG00000160796.12	chr3	+	47048555	47048645	47047808	47047939	47048730	47048840	0.05500	0.00024	included	K562	GPKOW
SE	C6orf48	ENSG00000204387.8	chr6	+	31804200	31804294	31803187	31803228	31805012	31805212	0.32800	0.00004	included	K562	GPKOW
SE	COPS3	ENSG00000141030.8	chr17	-	17164913	17165006	17163687	17163788	17165279	17165420	0.07300	0.00001	included	K562	GPKOW
SE	NDUFV3	ENSG00000160194.13	chr21	+	44323291	44324386	44317036	44317157	44328973	44329215	0.19800	0.00000	included	K562	GPKOW
SE	BAG6	ENSG00000204463.8	chr6	-	31607276	31607423	31606804	31607003	31607975	31608083	0.17900	0.00076	included	K562	GPKOW
SE	ANKHD1	ENSG00000131503.16	chr5	+	139914946	139915123	139907768	139909381	139916922	139917174	0.08900	0.00255	included	K562	GPKOW
SE	HMGN1	ENSG00000205581.6	chr21	-	40719841	40719878	40717755	40717884	40720217	40720265	0.44100	0.00072	included	K562	GPKOW
SE	HNRNPD	ENSG00000138668.14	chr4	-	83276047	83276154	83274986	83275307	83276456	83276554	0.10400	0.00000	included	K562	GPKOW

Showing 1 to 10 of 100 entries

2 RBPs can affect the splicing of GPKOW :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
A3SS	GPKOW	ENSG00000068394.9	chrX	-	48972244	48972442			48972244	48972368	48972574	48972677	0.08500	0.00000	0.00000	longer	K562	AQR
A3SS	GPKOW	ENSG00000068394.9	chrX	-	48972528	48972705			48972528	48972677	48973383	48973516	0.06300	0.00002	0.00370	longer	K562	AKAP8L

Showing 1 to 2 of 2 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

GPKOW

General Information

Functions

Localization(RBPImage: GPKOW)

Domains (AlphaFold: GPKOW)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

GPKOW protein has 1,128 RNA targets:

5 proteins are bound to GPKOW RNA:

No autoregularoty interactions between protein GPKOW and its RNA.

Diffrential Expression

12,245 differentially expressed genes after Knockdown/Knockout GPKOW protein :

39 RBPs can affect the gene expression of GPKOW :

285 alternative splicing events after Knockdown/Knockout GPKOW protein :

2 RBPs can affect the splicing of GPKOW :

No autoregularoty alternative splicing events between protein GPKOW and its RNA.