SMG5

General Information

Gene Name : SMG5
Gene ID : ENSG00000198952.8
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: SMG5)

Not Available

Antibody Validation

Bethyl Labs: A302-804A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	SMG5	ENSG00000198952.8	17.54	31.13	24.34	ENCSR000AEF
HepG2	SMG5	ENSG00000198952.8	53.90	42.44	48.17	ENCSR985KAT
K562	SMG5	ENSG00000198952.8	50.11	46.91	48.51	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	SMG5-BGKcLV33-K562		SMG5	K562	Released			CRISPR	KD% = no band on the position,
knockdown followed by RNA-seq	SMG5-BGKcLV33-K562	ENCSR183WOW	SMG5	K562	Released		ENCSR271ZAB	CRISPR	KD% = no band on the position,

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
SMG5	ENCAB166GHC	A302-804A	1					BGKcLV33-55, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
SMG5	K562	CRISPR			BGKcLV33-51,BGKcLV33-52	BGKcLV33-1,BGKcLV33-2	no band on the position,	Released	ENCODE4
SMG5	K562	CRISPR	ENCSR183WOW	ENCSR271ZAB	SMG5-BGKcLV33-55,SMG5-BGKcLV33-56	NT-BGKcLV33-1,NT-BGKcLV33-2	no band on the position,	Released	ENCODE4
SMG5	HepG2	CRISPR			BGHcLV31-51,BGHcLV31-52	BGHcLV31-1,BGHcLV31-2	0.0,0.0	NotSatisfied	ENCODE4
SMG5	HepG2	CRISPR			BGHcLV34-51,BGHcLV34-52	BGHcLV34-1,BGHcLV34-2	0.0,0.0	NotSatisfied	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
SMG5	Product_ID: A302-804A Lot_ID: 1 Source: Bethyl Labs Target Name: SMG5-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for SMG5 is not avaliable on ENCORE database.

Proteins bound to RNA

SMG5 RNA is bound by 37 proteins.

CanvasJS.com

eCLIP assay for SMG5 is not avaliable on ENCORE database.

37 proteins are bound to SMG5 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr1	156219235	156219390	-	3'-UTR,intron-Distal	5.89628	39.24249	K562	UPF1
chr1	156219071	156219232	-	3'-UTR,intron-Distal	5.82852	37.46656	K562	UPF1
chr1	156219926	156220045	-	3'-UTR,intron-Distal	5.71014	35.04290	K562	UPF1
chr1	156220076	156220234	-	3'-UTR,intron-Distal	4.40723	33.74352	K562	UPF1
chr1	156220781	156220863	-	CDS,intron-Distal,intron-Proximal	4.95845	32.01008	HepG2	SF3A3
chr1	156219668	156219765	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.58824	31.97885	K562	UPF1
chr1	156220088	156220201	-	3'-UTR,intron-Distal	3.70717	30.33726	HepG2	UPF1
chr1	156219520	156219598	-	3'-UTR,CDS,intron-Distal,intron-Proximal	5.40309	27.40392	K562	UPF1
chr1	156220089	156220173	-	3'-UTR,intron-Distal	4.50679	21.82906	HepG2	AKAP1
chr1	156220280	156220383	-	3'-UTR,CDS,intron-Distal	4.93055	19.29184	HepG2	AKAP1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 124 entries

eCLIP assay for SMG5 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for SMG5 is not available on ENCORE database.
75 RBPs can affect the levels of SMG5.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for SMG5 is not available on ENCORE database.

75 RBPs can affect the gene expression of SMG5 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SMG5	ENSG00000198952.7	3776.60780	2088.72580	0.85440	18.95130	16.58040	K562	MAGOH
SMG5	ENSG00000198952.7	2411.03970	1411.22400	0.77260	18.14680	16.04680	K562	EIF3G
SMG5	ENSG00000198952.7	3354.24520	2029.42920	0.72480	14.95550	13.47680	K562	PTBP1
SMG5	ENSG00000198952.7	763.23120	1799.64810	-1.23750	13.88840	12.16060	HepG2	SNRNP70
SMG5	ENSG00000198952.7	3313.73850	2305.34260	0.52340	11.42330	10.28040	K562	FXR1
SMG5	ENSG00000198952.7	2222.81680	3015.43720	-0.44000	10.59310	9.56380	HepG2	ACO1
SMG5	ENSG00000198952.7	3468.07300	2216.77200	0.64560	10.23740	8.64050	K562	EIF4A3
SMG5	ENSG00000198952.7	3041.13650	4585.97550	-0.59260	9.13850	8.17260	K562	AQR
SMG5	ENSG00000198952.7	621.96780	1306.81690	-1.07110	9.34490	7.88990	K562	G3BP2
SMG5	ENSG00000198952.7	3863.13860	2911.74270	0.40780	8.40170	7.30280	HepG2	SRSF7
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 82 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for SMG5 is not available on ENCORE database.

15 RBPs can affect the splicing of SMG5 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	SMG5	ENSG00000198952.7	chr1	-	156243354	156243439	156243151	156243241	156244387	156244477	0.09600	0.00000	0.00000	included	HepG2	HNRNPK
SE	SMG5	ENSG00000198952.7	chr1	-	156221193	156221268	156220648	156220787	156222204	156222295	-0.13100	0.00000	0.00003	excluded	K562	SUPV3L1
SE	SMG5	ENSG00000198952.7	chr1	-	156243354	156243439	156243151	156243241	156244387	156244477	0.08700	0.00000	0.00097	included	K562	CPSF6
SE	SMG5	ENSG00000198952.7	chr1	-	156243354	156243439	156243151	156243241	156244387	156244477	0.07600	0.00001	0.00306	included	K562	FMR1
SE	SMG5	ENSG00000198952.7	chr1	-	156243354	156243439	156243151	156243241	156244387	156244477	0.07500	0.00001	0.00490	included	K562	GPKOW
A3SS	SMG5	ENSG00000198952.7	chr1	-	156231123	156231231	156231123	156231199	156233185	156233361	0.06100	0.00001	0.00010	longer	K562	AQR
A3SS	SMG5	ENSG00000198952.7	chr1	-	156223239	156223318	156223239	156223299	156228795	156228925	0.05700	0.00003	0.00031	longer	K562	AQR
A3SS	SMG5	ENSG00000198952.7	chr1	-	156246843	156247056	156246843	156247032	156247715	156247839	0.05600	0.00005	0.00047	longer	K562	AQR
A3SS	SMG5	ENSG00000198952.7	chr1	-	156248726	156248958	156248726	156248825	156252397	156252620	0.08100	0.00010	0.00090	longer	K562	AQR
A3SS	SMG5	ENSG00000198952.7	chr1	-	156247715	156247878	156247715	156247839	156248726	156248958	0.05200	0.00014	0.00119	longer	K562	AQR

Showing 1 to 10 of 24 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

SMG5

General Information

Functions

Localization

Domains (AlphaFold: SMG5)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for SMG5 is not avaliable on ENCORE database.

37 proteins are bound to SMG5 RNA:

eCLIP assay for SMG5 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for SMG5 is not available on ENCORE database.

75 RBPs can affect the gene expression of SMG5 :

The Knockdown/knockout RNA-Seq assay for SMG5 is not available on ENCORE database.

15 RBPs can affect the splicing of SMG5 :

The Knockdown/knockout RNA-Seq assay for SMG5 is not avaliable on ENCORE database.