EIF3H

General Information

Gene Name : EIF3H
Gene ID : ENSG00000147677.10
Essential Gene : Yes

Functions

Ribosome & basic translation
Translation regulation

Localization(RBPImage: EIF3H)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Speckles
Cytoplasm
Cytosolic
Mitotic-Apparatus (Spindle; Centrosomes)

Domains (AlphaFold: EIF3H)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A301-754A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	EIF3H	ENSG00000147677.10	437.03	469.51	453.27	ENCSR000AEF
HepG2	EIF3H	ENSG00000147677.10	265.57	276.57	271.07	ENCSR985KAT
K562	EIF3H	ENSG00000147677.10	382.55	433.73	408.14	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 545	ENCSR916XIV	EIF3H	HepG2	Released	ENCAB957ITF	ENCSR756NPZ
knockdown followed by RNA-seq	EIF3H-BGKcLV04-K562	ENCSR096WVE	EIF3H	K562	Released		ENCSR226KWO	CRISPR	KD% = 69.1,73.6
knockdown followed by RNA-seq	EIF3H-BGHcLV05-HepG2	ENCSR733MVT	EIF3H	HepG2	Released		ENCSR805TIZ	CRISPR	KD% = 68.8,73.9

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
EIF3H	545	HepG2	ENCSR916XIV	ENCSR756NPZ	ENCAB957ITF	A301-754A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
EIF3H	ENCAB957ITF	A301-754A	1		Released, ENCODE3	Released, ENCODE3	BGHcLV05-15, Released, ENCODE3	BGKcLV04-11, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
EIF3H	K562	shRNA			BGKLV29-29,BGKLV29-30	BGKLV29-1,BGKLV29-2	32.4,36.0	NotSatisfied	ENCODE3
EIF3H	HepG2	shRNA			BGHLV30-33,BGHLV30-34	BGHLV30-1,BGHLV30-2	18.7,27.1	NotSatisfied	ENCODE3
EIF3H	K562	CRISPR	ENCSR096WVE	ENCSR226KWO	EIF3H_BGKcLV04_11B,EIF3H_BGKcLV04_12B	NT_BGKcLV04-1B,NT_BGKcLV04-2B	69.1,73.6	Released	ENCODE3
EIF3H	K562	CRISPR			BGKcLV04-11-D12,BGKcLV04-12-D12	BGKcLV04-1,BGKcLV04-2		NotSatisfied	ENCODE3
EIF3H	K562	CRISPR			BGKcLV04-12-D12,BGHcLV05-15	BGKcLV04-1,BGKcLV04-2	,68.8	NotSatisfied	ENCODE3
EIF3H	HepG2	CRISPR	ENCSR733MVT	ENCSR805TIZ	EIF3H-BGHcLV05-15D,EIF3H-BGHcLV05-16D	NT-BGHcLV05-1D,NT-BGHcLV05-2D	68.8,73.9	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

EIF3H: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Speckles

Cytoplasm

Cytosolic

Mitotic-Apparatus (Spindle; Centrosomes)

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
EIF3H	Product_ID: A301-754A Lot_ID: 1 Source: Bethyl Labs Target Name: EIF3H-human

RNA Targets

EIF3H protein binds to 2,901 RNA regions.

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Proteins bound to RNA

EIF3H RNA is bound by 28 proteins.

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EIF3H protein has 2,901 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
EIF3H	chr2	62932936	62933000	+	3.36900	70.65728	HepG2	EHBP1	5'-UTR,intron-Distal,intron-Proximal
EIF3H	chr1	19490856	19490924	-	5.92136	57.46986	HepG2	UBR4	CDS,intron-Distal
EIF3H	chr2	21234841	21234897	-	3.43444	55.05146	HepG2	APOB	CDS,intron-Distal
EIF3H	chr9	116930648	116930687	+	3.65878	53.22281	HepG2	COL27A1	CDS
EIF3H	chr1	19444331	19444388	-	4.83588	34.07491	HepG2	UBR4	5'-UTR,CDS,intron-Distal
EIF3H	chr1	19474588	19474620	-	5.24530	33.28690	HepG2	UBR4	CDS,intron-Distal
EIF3H	chr1	19441936	19442005	-	4.55068	31.24586	HepG2	UBR4	CDS,intron-Distal
EIF3H	chr6	144835838	144835896	+	3.51770	25.18684	HepG2	UTRN	CDS
EIF3H	chr9	117038038	117038048	+	3.07820	24.49876	HepG2	COL27A1	3'-UTR,CDS
EIF3H	chr16	18479317	18479430	-	5.56754	23.55979	HepG2	PKD1P5, RP11-1212A22.1	intron-Distal,intron-Proximal

Showing 1 to 10 of 100 entries

28 proteins are bound to EIF3H RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr8	117668125	117668186	-	CDS,intron-Distal,intron-Proximal	4.63734	67.12097	K562	PUM2
chr8	117671133	117671154	-	3'-UTR,CDS,intron-Distal	3.47975	59.85338	K562	YBX3
chr8	117657110	117657164	-	3'-UTR,intron-Distal	6.80330	52.72658	HepG2	SUB1
chr8	117671154	117671177	-	3'-UTR,CDS,intron-Distal	3.38494	52.47551	K562	YBX3
chr8	117671126	117671133	-	3'-UTR,CDS,intron-Distal	3.33526	42.79463	K562	YBX3
chr8	117657087	117657110	-	3'-UTR,intron-Distal	7.93045	41.84651	HepG2	SUB1
chr8	117671117	117671123	-	3'-UTR,CDS,intron-Distal,intron-Proximal	3.29346	41.61361	K562	YBX3
chr8	117671113	117671117	-	3'-UTR,CDS,intron-Distal,intron-Proximal	3.54333	41.50692	K562	YBX3
chr8	117658779	117658808	-	CDS,intron-Distal,intron-Proximal	3.30198	40.79102	K562	YBX3
chr8	117738278	117738318	-	5'-UTR,CDS,intron-Distal,intron-Proximal	3.45156	38.53664	K562	YBX3
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 113 entries

No autoregularoty interactions between protein EIF3H and its RNA.

Diffrential Expression

495 differentially expressed genes after Knockdown/Knockout EIF3H protein.

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100 RBPs can affect the levels of EIF3H.

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495 differentially expressed genes after Knockdown/Knockout EIF3H protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SPARC	ENSG00000113140.6	455.37610	871.83680	-0.93700	9.18550	5.73030	HepG2	EIF3H
EIF5	ENSG00000100664.6	5249.53450	3238.89450	0.69660	8.39780	5.14080	HepG2	EIF3H
WFS1	ENSG00000109501.9	858.05930	1397.46380	-0.70370	7.05560	4.00680	HepG2	EIF3H
SCNN1A	ENSG00000111319.8	774.81690	1239.07460	-0.67730	6.83530	3.82610	HepG2	EIF3H
MCM2	ENSG00000073111.9	215.78680	404.13680	-0.90520	6.65870	3.70270	HepG2	EIF3H
MED15	ENSG00000099917.13	1096.35860	1701.44350	-0.63400	6.65940	3.70270	HepG2	EIF3H
PFKFB4	ENSG00000114268.7	2562.95540	3850.29040	-0.58720	6.56940	3.63400	HepG2	EIF3H
EVC	ENSG00000072840.8	378.35370	652.19210	-0.78560	6.32050	3.46690	HepG2	EIF3H
PCOLCE	ENSG00000106333.8	297.81840	517.24810	-0.79640	6.33830	3.46690	HepG2	EIF3H
ZMIZ1	ENSG00000108175.12	3410.75410	5025.20130	-0.55910	6.08510	3.30520	HepG2	EIF3H
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

100 RBPs can affect the gene expression of EIF3H :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
EIF3H	ENSG00000147677.6	3723.32360	12441.43140	-1.74050	58.91260	55.10830	K562	EIF3H
EIF3H	ENSG00000147677.6	3228.46130	6424.71410	-0.99280	51.13460	49.42420	HepG2	PTBP1
EIF3H	ENSG00000147677.6	1092.75520	2948.57240	-1.43200	44.11170	42.16250	HepG2	HNRNPA1
EIF3H	ENSG00000147677.6	4081.01390	6981.87910	-0.77470	38.84280	37.52100	HepG2	SRSF1
EIF3H	ENSG00000147677.6	1255.63910	3844.45250	-1.61440	37.40530	33.03430	HepG2	EIF3H
EIF3H	ENSG00000147677.6	3911.83630	6689.04560	-0.77400	34.36180	32.69850	HepG2	SF3B4
EIF3H	ENSG00000147677.6	2436.36340	4384.20620	-0.84760	30.56000	28.90040	HepG2	CELF1
EIF3H	ENSG00000147677.6	4356.27330	7150.13120	-0.71490	29.34480	27.97270	HepG2	NELFE
EIF3H	ENSG00000147677.6	3948.14230	6958.22970	-0.81750	26.78550	25.13140	HepG2	QKI
EIF3H	ENSG00000147677.6	1402.37480	3463.95200	-1.30450	26.53010	24.78400	HepG2	GPKOW
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 117 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

33 alternative splicing events after Knockdown/Knockout EIF3H protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	SF3B1	ENSG00000115524.11	chr2	-	198283619	198283675			198283232	198283312	198285151	198285266	0.08200	0.00000	0.01148	included	HepG2	EIF3H
SE	RPS6KB1	ENSG00000108443.9	chr17	+	58007718	58007793			58007477	58007535	58008982	58009053	0.14100	0.00000	0.00328	included	HepG2	EIF3H
SE	RBM39	ENSG00000131051.16	chr20	-	34328446	34328519			34326889	34326939	34328745	34328809	0.15000	0.00000	0.00762	included	HepG2	EIF3H
SE	EIF3H	ENSG00000147677.6	chr8	-	117738254	117738411			117671051	117671219	117767904	117768036	-0.44300	0.00000	0.00000	excluded	HepG2	EIF3H
MXE	EIF3H	ENSG00000147677.6	chr8	-	117668094	117668244	117738254	117738411	117661044	117661165	117767904	117768060	-0.11700	0.00000	0.00011	2included	HepG2	EIF3H
MXE	NDUFA10	ENSG00000130414.7	chr2	-	240951033	240954277	240957969	240958056	240946732	240946787	240960613	240960829	-0.12000	0.00000	0.00256	2included	HepG2	EIF3H
MXE	SERPINA1	ENSG00000197249.8	chr14	-	94845800	94845948	94848928	94849578	94844725	94844977	94854896	94854997	-0.20600	0.00000	0.00000	2included	HepG2	EIF3H
SE	ANKRD10	ENSG00000088448.10	chr13	-	111552876	111553008			111545303	111545610	111558379	111558471	0.24400	0.00001	0.03573	included	HepG2	EIF3H
SE	FN1	ENSG00000115414.14	chr2	-	216245533	216245803			216243852	216244040	216246934	216247048	0.09800	0.00001	0.01657	included	HepG2	EIF3H
SE	EVA1A	ENSG00000115363.9	chr2	-	75753237	75753325			75749490	75749613	75787805	75788064	0.22700	0.00001	0.03573	included	HepG2	EIF3H

Showing 1 to 10 of 33 entries

22 RBPs can affect the splicing of EIF3H :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	EIF3H	ENSG00000147677.6	chr8	-	117668094	117668244	117658709	117658842	117767904	117768060	-0.46900	0.00001	excluded	K562	AKAP8L
SE	EIF3H	ENSG00000147677.6	chr8	-	117661044	117661165	117658709	117658842	117767904	117768036	-0.13000	0.00000	excluded	K562	AQR
SE	EIF3H	ENSG00000147677.6	chr8	-	117738254	117738411	117658709	117658842	117767904	117768036	-0.13200	0.00000	excluded	K562	PUS1
SE	EIF3H	ENSG00000147677.6	chr8	-	117738254	117738411	117658709	117658842	117767904	117768060	-0.09100	0.00001	excluded	HepG2	SF3A3
SE	EIF3H	ENSG00000147677.6	chr8	-	117738254	117738411	117671051	117671219	117767904	117768036	-0.44300	0.00000	excluded	HepG2	EIF3H
SE	EIF3H	ENSG00000147677.6	chr8	-	117668094	117668244	117658709	117658842	117767904	117768060	-0.44800	0.00006	excluded	K562	UTP18
SE	EIF3H	ENSG00000147677.6	chr8	-	117738254	117738411	117658709	117658842	117767904	117768060	-0.05800	0.00000	excluded	K562	RBM15
SE	EIF3H	ENSG00000147677.6	chr8	-	117749475	117749526	117738254	117738411	117767904	117768068	0.06000	0.00000	included	K562	HNRNPC
SE	EIF3H	ENSG00000147677.6	chr8	-	117738254	117738411	117671051	117671219	117767904	117768060	-0.06400	0.00000	excluded	K562	HNRNPC
A3SS	EIF3H	ENSG00000147677.6	chr8	-	117661044	117661263	117661044	117661165	117668094	117668301	0.08400	0.00000	longer	K562	AQR

Showing 1 to 10 of 36 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The EIF3H protein affects to its own RNA splicing after knockdown/knockout.

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	EIF3H	ENSG00000147677.6	chr8	-	117738254	117738411			117671051	117671219	117767904	117768036	-0.44300	0.00000	0.00000	excluded	HepG2	EIF3H
MXE	EIF3H	ENSG00000147677.6	chr8	-	117668094	117668244	117738254	117738411	117661044	117661165	117767904	117768060	-0.11700	0.00000	0.00011	2included	HepG2	EIF3H
MXE	EIF3H	ENSG00000147677.6	chr8	-	117661044	117661165	117738254	117738411	117658709	117658842	117767904	117768036	-0.10800	0.00002	0.01549	2included	HepG2	EIF3H

Showing 1 to 3 of 3 entries