NXF1

General Information

Gene Name : NXF1
Gene ID : ENSG00000162231.13
Essential Gene : Yes

Functions

RNA export
Viral RNA regulation

Localization(RBPImage: NXF1)

Nuclei
Nucleoplasm
Cytoplasm
Cytosolic

Domains (AlphaFold: NXF1)

RRM

Antibody Validation

Bethyl Labs: A303-914A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	NXF1	ENSG00000162231.13	47.31	57.76	52.53	ENCSR000AEF
HepG2	NXF1	ENSG00000162231.13	70.17	57.57	63.87	ENCSR985KAT
K562	NXF1	ENSG00000162231.13	62.75	66.10	64.42	ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
NXF1		A303-914A	1				BGHcLV47-47, Experimented, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Sample_reps	Control_reps	KD%	Status	Project
NXF1	K562	shRNA	BGKLV13-31,BGKLV13-32	BGKLV13-1,BGKLV13-2	no band,no band	NotSatisfied	ENCODE3
NXF1	K562	shRNA	BGKLV25-37,BGKLV25-38	BGKLV25-1,BGKLV25-2	redo transduction,redo transduction	NotSatisfied	ENCODE3
NXF1	HepG2	shRNA	BGHLV23-27,BGHLV23-28	BGHLV23-1,BGHLV23-2	redo transfection,redo transfection	NotSatisfied	ENCODE3
NXF1	K562	CRISPR	NXF1-BGKcLV46-49,NXF1-BGKcLV46-50	NT-BGKcLV46-1,NT-BGKcLV46-2	68.2,68.8	Submitting	ENCORE2
NXF1	K562	CRISPR	BGKcLV46-51,BGKcLV46-52	BGKcLV46-1,BGKcLV46-2	19.5,-0.8	NotSatisfied	ENCORE2
NXF1	HepG2	CRISPR	BGHcLV47-47,BGHcLV47-48	BGHcLV47-1,BGHcLV47-2	81.9,84.9	Sequenced	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

NXF1: Click to RBP Image Database

Nuclei

Nucleoplasm

Cytoplasm

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
NXF1	Product_ID: A303-914A Lot_ID: 1 Source: Bethyl Labs Target Name: NXF1-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for NXF1 is not avaliable on ENCORE database.

Proteins bound to RNA

NXF1 RNA is bound by 28 proteins.

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eCLIP assay for NXF1 is not avaliable on ENCORE database.

28 proteins are bound to NXF1 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr11	62567591	62567613	-	3'-UTR,5'-UTR,intron-Distal	7.34039	88.50129	K562	PTBP1
chr11	62567552	62567591	-	3'-UTR,5'-UTR,intron-Distal,intron-Proximal	7.26615	84.31066	K562	PTBP1
chr11	62566581	62566686	-	5'-UTR,intron-Distal	5.29814	54.63145	HepG2	PTBP1
chr11	62566491	62566548	-	5'-UTR,intron-Distal	4.96315	50.53377	K562	PTBP1
chr11	62567557	62567650	-	3'-UTR,5'-UTR,intron-Distal,intron-Proximal	4.29140	43.03884	HepG2	PTBP1
chr11	62566477	62566549	-	5'-UTR,intron-Distal	5.34784	40.86322	HepG2	PTBP1
chr11	62566576	62566632	-	5'-UTR,intron-Distal	5.72798	39.29054	K562	PTBP1
chr11	62567613	62567654	-	3'-UTR,5'-UTR,intron-Distal	5.56833	28.68274	K562	PTBP1
chr11	62559850	62559949	-	3'-UTR,intron-Distal,intron-Proximal	5.88036	26.60356	K562	PUM2
chr11	62562371	62562407	-	intron-Distal,intron-Proximal	4.63303	24.55500	K562	AQR
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 115 entries

eCLIP assay for NXF1 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for NXF1 is not available on ENCORE database.
122 RBPs can affect the levels of NXF1.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for NXF1 is not available on ENCORE database.

122 RBPs can affect the gene expression of NXF1 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
NXF1	ENSG00000162231.9	11351.46660	1881.44590	2.59290	147.85830	144.20100	K562	AQR
NXF1	ENSG00000162231.9	2210.71210	740.85780	1.57710	69.58820	66.85110	K562	FXR1
NXF1	ENSG00000162231.9	2903.23150	1300.22340	1.15880	49.65490	48.02790	HepG2	NELFE
NXF1	ENSG00000162231.9	1879.07350	789.97280	1.25000	28.59030	25.75060	K562	RBM22
NXF1	ENSG00000162231.9	2611.77320	1549.41300	0.75320	26.35860	24.18110	K562	WDR43
NXF1	ENSG00000162231.9	2165.73200	1216.33910	0.83220	24.87700	23.40410	HepG2	SF3B4
NXF1	ENSG00000162231.9	3596.01830	1958.73920	0.87640	22.00440	20.33900	K562	DDX47
NXF1	ENSG00000162231.9	7150.25720	2695.72180	1.40720	22.46380	20.05180	K562	PUF60
NXF1	ENSG00000162231.9	1620.67650	874.78870	0.88950	20.68060	18.45170	K562	EIF3G
NXF1	ENSG00000162231.9	1463.95040	712.39060	1.03900	19.40260	17.01070	K562	EIF4A3
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 144 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for NXF1 is not available on ENCORE database.

17 RBPs can affect the splicing of NXF1 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	NXF1	ENSG00000162231.9	chr11	-	62568565	62568635	62566010	62567958	62568800	62568921	-0.15900	0.00005	excluded	K562	AQR
A3SS	NXF1	ENSG00000162231.9	chr11	-	62563537	62563653	62563537	62563630	62563758	62563817	-0.25100	0.00005	shorter	K562	SF3B4
A3SS	NXF1	ENSG00000162231.9	chr11	-	62564789	62565007	62564789	62564858	62566010	62566102	0.12600	0.00000	longer	HepG2	U2AF2
A3SS	NXF1	ENSG00000162231.9	chr11	-	62564789	62565007	62564789	62564858	62566010	62566107	0.10500	0.00000	longer	K562	AQR
A3SS	NXF1	ENSG00000162231.9	chr11	-	62568800	62568921	62568800	62568889	62569033	62569132	0.05600	0.00000	longer	K562	AQR
A3SS	NXF1	ENSG00000162231.9	chr11	-	62561809	62562023	62561809	62561912	62562406	62562513	0.18700	0.00000	longer	K562	AQR
A3SS	NXF1	ENSG00000162231.9	chr11	-	62562406	62562570	62562406	62562479	62563372	62563472	0.11600	0.00000	longer	K562	AQR
A3SS	NXF1	ENSG00000162231.9	chr11	-	62567848	62568076	62567848	62567958	62568565	62568635	0.27300	0.00000	longer	K562	AQR
A3SS	NXF1	ENSG00000162231.9	chr11	-	62559889	62560018	62559889	62559986	62560112	62560196	0.09200	0.00000	longer	K562	AQR
A3SS	NXF1	ENSG00000162231.9	chr11	-	62570890	62571134	62570890	62571044	62571263	62571450	0.12800	0.00000	longer	K562	AQR

Showing 1 to 10 of 39 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

NXF1

General Information

Functions

Localization(RBPImage: NXF1)

Domains (AlphaFold: NXF1)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for NXF1 is not avaliable on ENCORE database.

28 proteins are bound to NXF1 RNA:

eCLIP assay for NXF1 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for NXF1 is not available on ENCORE database.

122 RBPs can affect the gene expression of NXF1 :

The Knockdown/knockout RNA-Seq assay for NXF1 is not available on ENCORE database.

17 RBPs can affect the splicing of NXF1 :

The Knockdown/knockout RNA-Seq assay for NXF1 is not avaliable on ENCORE database.