GRSF1

General Information

Gene Name : GRSF1
Gene ID : ENSG00000132463.13
Essential Gene : Yes

Functions

RNA export
mitochondrial RNA regulation
Viral RNA regulation

Localization(RBPImage: GRSF1)

Cytoplasm
Compartment/Organelle
Mitochondria

Domains (AlphaFold: GRSF1)

RRM

Antibody Validation

MBLI: RN050PW

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	GRSF1	ENSG00000132463.13	54.64	50.62	52.63	ENCSR000AEF
HepG2	GRSF1	ENSG00000132463.13	45.07	68.11	56.59	ENCSR985KAT
K562	GRSF1	ENSG00000132463.13	54.22	58.86	56.54	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 596	ENCSR668MJX	GRSF1	HepG2	Released	ENCAB438AYW	ENCSR638YHN
knockdown followed by RNA-seq	GRSF1-BGKLV29-K562	ENCSR835RMN	GRSF1	K562	Released		ENCSR118EFE	shRNA	KD% = 80.5,76.4
knockdown followed by RNA-seq	GRSF1-BGHLV22-HepG2	ENCSR674KDQ	GRSF1	HepG2	Released		ENCSR279HMU	shRNA	KD% = 59.1,48.4

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
GRSF1	596	HepG2	ENCSR668MJX	ENCSR638YHN	ENCAB438AYW	RN050PW	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
GRSF1	ENCAB438AYW	RN050PW	1		Released, ENCODE3	Released, ENCODE3	BGHLV22-17, Released, ENCODE3	BGKLV29-37, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
GRSF1	K562	shRNA	ENCSR835RMN	ENCSR118EFE	GRSF1_BGKLV29-37B,GRSF1_BGKLV29-38B	NTB_BGKLV29-1,NTB_BGKLV29-2	80.5,76.4	Released	ENCODE3
GRSF1	HepG2	shRNA	ENCSR674KDQ	ENCSR279HMU	GRSF1-BGHLV22-17_17754,GRSF1-BGHLV22-18_17755	non-target-BGHLV22-1_17746,non-target-BGHLV22-2_17747	59.1,48.4	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

GRSF1: Click to RBP Image Database

Cytoplasm

Compartment/Organelle

Mitochondria

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
GRSF1	Product_ID: RN050PW Lot_ID: 1 Source: MBLI Target Name: GRSF1-human

RNA Targets

GRSF1 protein binds to 1,018 RNA regions.

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Proteins bound to RNA

GRSF1 RNA is bound by 22 proteins.

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GRSF1 protein has 1,018 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
GRSF1	chr5	134260254	134260256	+	10.10195	400.00000	HepG2	PCBD2	intron-Distal
GRSF1	chrM	14316	14322	-	11.37021	400.00000	HepG2	MT-ND6	CDS
GRSF1	chr5	134262721	134262739	+	9.51645	400.00000	HepG2	PCBD2	intron-Distal
GRSF1	chr9	136229561	136229586	-	6.71805	400.00000	HepG2	SURF4	3'-UTR,intron-Distal
GRSF1	chrM	14485	14487	-	10.56700	400.00000	HepG2	MT-ND6	CDS
GRSF1	chr14	32954283	32954312	+	7.90242	252.38444	HepG2	AKAP6	intron-Distal
GRSF1	chr16	9185695	9185728	+	5.16871	42.78236	HepG2	C16orf72	5'-UTR
GRSF1	chr12	53421607	53421634	+	5.25292	41.85817	HepG2	EIF4B	3'-UTR,CDS,intron-Distal
GRSF1	chr5	134263417	134263426	+	7.30876	41.82217	HepG2	PCBD2	intron-Distal
GRSF1	chr20	17639657	17639674	-	4.33661	39.37227	HepG2	RRBP1	CDS,intron-Distal,intron-Proximal

Showing 1 to 10 of 100 entries

22 proteins are bound to GRSF1 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr4	71698021	71698168	-	CDS,intron-Distal,intron-Proximal	3.53222	15.57419	K562	UCHL5
chr4	71693817	71693912	-	intron-Distal,intron-Proximal	5.90351	12.96053	K562	KHSRP
chr4	71705535	71705597	-	5'-UTR,CDS,intron-Distal	5.98048	12.51207	HepG2	DDX3X
chr4	71698832	71698898	-	CDS,intron-Distal,intron-Proximal	4.84992	12.44336	HepG2	BCLAF1
chr4	71705477	71705535	-	CDS,intron-Distal	4.96239	12.43092	HepG2	DDX3X
chr4	71698016	71698103	-	CDS,intron-Distal,intron-Proximal	4.16171	12.07231	HepG2	BCLAF1
chr4	71695925	71696010	-	intron-Distal	5.28638	10.28730	K562	TARDBP
chr4	71698103	71698170	-	CDS,intron-Distal,intron-Proximal	3.72251	10.26109	HepG2	BCLAF1
chr4	71691068	71691161	-	3'-UTR,CDS,intron-Distal,intron-Proximal	3.06916	10.11770	HepG2	U2AF2
chr4	71696687	71696780	-	intron-Distal	5.72765	8.94626	HepG2	QKI
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 48 entries

No autoregularoty interactions between protein GRSF1 and its RNA.

Diffrential Expression

8,479 differentially expressed genes after Knockdown/Knockout GRSF1 protein.

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109 RBPs can affect the levels of GRSF1.

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8,479 differentially expressed genes after Knockdown/Knockout GRSF1 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
KITLG	ENSG00000049130.9	1115.73140	289.02540	1.94860	29.33760	26.03200	HepG2	GRSF1
CDH1	ENSG00000039068.14	214.22070	711.33360	-1.73140	20.96660	18.11330	HepG2	GRSF1
PIK3CB	ENSG00000051382.4	920.99860	2338.48970	-1.34430	18.09440	15.34500	HepG2	GRSF1
NRXN3	ENSG00000021645.13	66.95340	0.54100	6.95120	17.71700	15.01350	HepG2	GRSF1
GAS7	ENSG00000007237.14	256.50230	48.47450	2.40360	16.74620	14.11730	HepG2	GRSF1
VCAN	ENSG00000038427.11	6848.99890	3154.56670	1.11840	14.24490	11.76320	HepG2	GRSF1
AGPAT4	ENSG00000026652.9	120.18860	17.76560	2.75800	13.94260	11.49730	HepG2	GRSF1
C8B	ENSG00000021852.8	93.13910	304.68440	-1.70990	13.79530	11.37690	HepG2	GRSF1
PDK4	ENSG00000004799.7	81.17780	13.47390	2.59080	9.40350	7.36510	HepG2	GRSF1
RNASET2	ENSG00000026297.11	3592.69950	7946.35990	-1.14520	9.00250	7.01570	HepG2	GRSF1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

109 RBPs can affect the gene expression of GRSF1 :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
GRSF1	ENSG00000132463.9	759.51090	4019.68180	-2.40390	126.98890	122.55890	K562	GRSF1
GRSF1	ENSG00000132463.9	1042.48110	2471.51300	-1.24540	42.46380	40.54990	HepG2	QKI
GRSF1	ENSG00000132463.9	1177.79910	2271.09260	-0.94730	33.98290	31.70450	K562	MTPAP
GRSF1	ENSG00000132463.9	396.46650	1463.61670	-1.88430	30.12280	26.73800	HepG2	GRSF1
GRSF1	ENSG00000132463.9	708.73270	1568.49660	-1.14610	25.01340	23.29240	K562	HNRNPC
GRSF1	ENSG00000132463.9	908.49950	1623.54460	-0.83760	19.72460	18.03210	HepG2	DNAJC21
GRSF1	ENSG00000132463.9	719.48110	1489.09250	-1.04940	18.04360	16.47810	HepG2	TARDBP
GRSF1	ENSG00000132463.9	1202.28980	1849.99940	-0.62170	17.92150	16.24380	HepG2	ILF2
GRSF1	ENSG00000132463.9	2275.65930	3331.12130	-0.54970	13.76930	12.59170	HepG2	ETF1
GRSF1	ENSG00000132463.9	2298.34580	3930.21110	-0.77400	13.54600	12.38670	K562	AQR
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 131 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

114 alternative splicing events after Knockdown/Knockout GRSF1 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	UBE2D2	ENSG00000131508.11	chr5	+	138941828	138941992	138940750	138941400	138979956	138980020	0.12100	0.00381	included	HepG2	GRSF1
SE	SPG7	ENSG00000197912.9	chr16	+	89615387	89615744	89614410	89614521	89616901	89617017	0.11400	0.00016	included	HepG2	GRSF1
SE	APOC1	ENSG00000130208.5	chr19	+	45417928	45417990	45417503	45417746	45418128	45418206	0.12100	0.00011	included	HepG2	GRSF1
SE	PICALM	ENSG00000073921.13	chr11	-	85701292	85701442	85694908	85695016	85707868	85707972	0.17900	0.00190	included	HepG2	GRSF1
SE	ABCC2	ENSG00000023839.6	chr10	+	101579184	101579299	101578845	101579026	101590063	101590190	0.06800	0.00044	included	HepG2	GRSF1
SE	HNMT	ENSG00000150540.9	chr2	+	138724666	138724956	138722048	138722198	138758487	138758595	0.07600	0.00010	included	HepG2	GRSF1
SE	BPTF	ENSG00000171634.12	chr17	+	65925074	65925236	65924470	65924717	65925451	65925603	0.24200	0.00010	included	HepG2	GRSF1
SE	DHRS2	ENSG00000100867.10	chr14	+	24110874	24110988	24109002	24109104	24112360	24112428	0.06000	0.00003	included	HepG2	GRSF1
SE	C17orf76-AS1	ENSG00000175061.13	chr17	+	16342841	16343006	16342634	16342728	16343498	16343567	0.12800	0.00194	included	HepG2	GRSF1
SE	FKBP10	ENSG00000141756.14	chr17	+	39976092	39976275	39975781	39975927	39976520	39976713	0.06300	0.00138	included	HepG2	GRSF1

Showing 1 to 10 of 100 entries

25 RBPs can affect the splicing of GRSF1 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	GRSF1	ENSG00000132463.9	chr4	-	71697255	71697391	71693568	71693753	71698023	71698167	-0.11700	0.00000	0.00000	excluded	K562	AQR
A3SS	GRSF1	ENSG00000132463.9	chr4	-	71697255	71697436	71697255	71697391	71698023	71698167	0.05800	0.00000	0.00000	longer	K562	AQR
A3SS	GRSF1	ENSG00000132463.9	chr4	-	71691012	71691207	71691012	71691148	71691840	71692001	0.09800	0.00000	0.00000	longer	K562	AQR
RI	GRSF1	ENSG00000132463.9	chr4	-	71691012	71691962	71691012	71691148	71691840	71691962	0.13600	0.00000	0.00000	included	HepG2	PRPF8
RI	GRSF1	ENSG00000132463.9	chr4	-	71691012	71691962	71691012	71691148	71691840	71691962	0.16300	0.00000	0.00015	included	HepG2	GRSF1
RI	GRSF1	ENSG00000132463.9	chr4	-	71691012	71691962	71691012	71691148	71691840	71691962	0.17000	0.00000	0.00000	included	HepG2	QKI
RI	GRSF1	ENSG00000132463.9	chr4	-	71691012	71691962	71691012	71691148	71691840	71691962	0.11900	0.00000	0.00010	included	HepG2	TIAL1
RI	GRSF1	ENSG00000132463.9	chr4	-	71691012	71691962	71691012	71691148	71691840	71691962	0.11100	0.00000	0.00000	included	HepG2	NCBP2
RI	GRSF1	ENSG00000132463.9	chr4	-	71691012	71691962	71691012	71691148	71691840	71691962	0.15800	0.00000	0.00000	included	K562	AQR
SE	GRSF1	ENSG00000132463.9	chr4	-	71697255	71697391	71693568	71693753	71698023	71698167	-0.08500	0.00001	0.00052	excluded	K562	U2AF1

Showing 1 to 10 of 33 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The GRSF1 protein affects to its own RNA splicing after knockdown/knockout.

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
RI	GRSF1	ENSG00000132463.9	chr4	-	71691012	71691962			71691012	71691148	71691840	71691962	0.16300	0.00000	0.00015	included	HepG2	GRSF1

Showing 1 to 1 of 1 entries