CSTF2

General Information

Gene Name : CSTF2
Gene ID : ENSG00000101811.13
Essential Gene : No

Functions

3' end processing

Localization(RBPImage: CSTF2)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Speckles
Nuclear release mitosis

Domains (AlphaFold: CSTF2)

RRM

Antibody Validation

Bethyl Labs: A301-092A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	CSTF2	ENSG00000101811.13	25.35	25.28	25.32	ENCSR000AEF
HepG2	CSTF2	ENSG00000101811.13	26.96	31.30	29.13	ENCSR985KAT
K562	CSTF2	ENSG00000101811.13	36.59	36.67	36.63	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 572	ENCSR384MWO	CSTF2	HepG2	Released	ENCAB117EBX	ENCSR309GHK
knockdown followed by RNA-seq	CSTF2-BGKLV19-K562	ENCSR885YOI	CSTF2	K562	Released		ENCSR245BNJ	shRNA	KD% = 71.2,70.7
knockdown followed by RNA-seq	CSTF2-BGHLV17-HepG2	ENCSR815JDY	CSTF2	HepG2	Released		ENCSR856ZRV	shRNA	KD% = 91.0,86.3

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
CSTF2	572	HepG2	ENCSR384MWO	ENCSR309GHK	ENCAB117EBX	A301-092A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
CSTF2	ENCAB117EBX	A301-092A	1		Released, ENCODE3	Released, ENCODE3	BGHLV17-9, Released, ENCODE3	BGKLV19-9, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
CSTF2	K562	shRNA			BGKLV08-9,BGKLV08-10	BGKLV08-1,BGKLV08-2		NotSatisfied	ENCODE3
CSTF2	K562	shRNA	ENCSR885YOI	ENCSR245BNJ	CSTF2_BGKLV19_9A,CSTF2_BGKLV19_10A	NT_BGKLV19_1A,NT_BGKLV19_2A	71.2,70.7	Released	ENCODE3
CSTF2	HepG2	shRNA			BGHLV16-11,BGHLV16-12	BGHLV16-1,BGHLV16-2		NotSatisfied	ENCODE3
CSTF2	HepG2	shRNA	ENCSR815JDY	ENCSR856ZRV	CSTF2_BGHLV17_9,CSTF2_BGHLV17_10	NT_BGHLV17_63,NT_BGHLV17_64	91.0,86.3	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

CSTF2: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Speckles

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
CSTF2	Product_ID: A301-092A Lot_ID: 1 Source: Bethyl Labs Target Name: CSTF2-human

RNA Targets

CSTF2 protein binds to 2,738 RNA regions.

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Proteins bound to RNA

CSTF2 RNA is bound by 7 proteins.

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CSTF2 protein has 2,738 RNA targets:

Only display 100 entries as example. Please click "View All" button to see all 2,738 entries.

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
CSTF2	chr11	2153259	2153278	-	3.79023	41.23550	HepG2	IGF2	3'-UTR,intron-Distal
CSTF2	chr11	2152823	2152835	-	4.52827	32.38979	HepG2	IGF2	3'-UTR,intron-Distal
CSTF2	chrX	108939647	108939673	-	5.81274	31.00718	HepG2	ACSL4	intron-Distal
CSTF2	chr14	31458558	31458651	-	5.02757	18.44320	HepG2	STRN3	intron-Distal
CSTF2	chr3	171905136	171905196	+	5.29153	17.60836	HepG2	FNDC3B	intron-Distal
CSTF2	chr20	40185315	40185398	-	5.54351	15.01087	HepG2	CHD6	intron-Distal
CSTF2	chr19	5894626	5894693	-	4.57327	14.48622	HepG2	FUT5, NDUFA11, AC024592.12	3'-UTR,intron-Distal,intron-Proximal,polyA_site
CSTF2	chr7	138156106	138156198	+	3.68555	14.35268	HepG2	TRIM24	intron-Distal
CSTF2	chr7	64459689	64459751	-	5.69495	13.77245	HepG2	ZNF117, ERV3-1	intron-Distal
CSTF2	chr4	140625462	140625497	+	5.75883	13.16417	HepG2	MGST2	intron-Distal

Showing 1 to 10 of 100 entries

7 proteins are bound to CSTF2 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chrX	100082977	100083070	+	CDS,intron-Proximal	5.70322	7.91936	K562	SF3B4
chrX	100075398	100075465	+	5'-UTR,CDS,intron-Proximal	4.21077	5.47892	K562	DDX3X
chrX	100083011	100083086	+	CDS,intron-Proximal	3.77493	4.36528	HepG2	U2AF2
chrX	100095823	100095905	+	3'-UTR	4.15535	3.95344	K562	PUM2
chrX	100088222	100088349	+	CDS,intron-Proximal	3.47606	3.69481	K562	UCHL5
chrX	100092779	100092862	+	intron-Distal	3.63614	3.06150	K562	KHSRP
chrX	100078304	100078388	+	CDS	3.63462	3.02250	K562	YBX3
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 7 of 7 entries

No autoregularoty interactions between protein CSTF2 and its RNA.

Diffrential Expression

5,011 differentially expressed genes after Knockdown/Knockout CSTF2 protein.

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69 RBPs can affect the levels of CSTF2.

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5,011 differentially expressed genes after Knockdown/Knockout CSTF2 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SLC7A2	ENSG00000003989.12	610.79160	2198.34630	-1.84770	56.20330	52.90070	HepG2	CSTF2
VCAN	ENSG00000038427.11	562.38400	2095.19890	-1.89750	36.24850	33.40960	HepG2	CSTF2
RRM2B	ENSG00000048392.7	1887.04690	739.53010	1.35130	32.32350	29.58160	HepG2	CSTF2
RNASET2	ENSG00000026297.11	558.33220	1300.91390	-1.22030	22.59210	20.08060	HepG2	CSTF2
CP	ENSG00000047457.9	3766.27350	10999.36240	-1.54620	19.84600	17.47050	HepG2	CSTF2
JHDM1D	ENSG00000006459.6	336.64920	785.79380	-1.22290	17.53950	15.25240	HepG2	CSTF2
ANLN	ENSG00000011426.6	751.88810	1519.40540	-1.01490	17.00340	14.72390	HepG2	CSTF2
CX3CL1	ENSG00000006210.6	2045.59120	1040.11450	0.97570	16.03330	13.81310	HepG2	CSTF2
FAS	ENSG00000026103.15	448.98340	191.18260	1.23160	14.43370	12.31500	HepG2	CSTF2
USP2	ENSG00000036672.11	414.46760	162.93110	1.34690	13.96860	11.87990	HepG2	CSTF2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

69 RBPs can affect the gene expression of CSTF2 :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
CSTF2	ENSG00000101811.9	134.37440	635.27670	-2.24110	44.61600	40.95020	K562	CSTF2
CSTF2	ENSG00000101811.9	987.49780	482.98560	1.03170	21.18150	19.61710	K562	FXR1
CSTF2	ENSG00000101811.9	1014.76880	381.61600	1.41090	19.01470	17.74890	HepG2	AKAP1
CSTF2	ENSG00000101811.9	1077.71090	537.79990	1.00270	17.51330	15.75600	K562	SRSF1
CSTF2	ENSG00000101811.9	65.48890	247.20870	-1.91640	17.58850	15.29360	HepG2	CSTF2
CSTF2	ENSG00000101811.9	674.44200	340.86880	0.98440	16.00050	14.73450	HepG2	UTP3
CSTF2	ENSG00000101811.9	676.25650	348.49070	0.95640	14.22610	13.33240	HepG2	SRSF1
CSTF2	ENSG00000101811.9	321.76430	577.40660	-0.84360	11.22690	9.98780	HepG2	SRSF7
CSTF2	ENSG00000101811.9	628.51250	259.42120	1.27650	10.49710	9.03790	HepG2	CNOT7
CSTF2	ENSG00000101811.9	903.37560	548.55430	0.71960	9.58690	8.40550	K562	PTBP1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 79 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

20 alternative splicing events after Knockdown/Knockout CSTF2 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	MYL6	ENSG00000092841.14	chr12	+	56554409	56554454	56554026	56554104	56555170	56555371	0.17300	0.00000	included	HepG2	CSTF2
SE	ZNF207	ENSG00000010244.12	chr17	+	30693683	30693776	30692348	30692506	30694790	30695033	0.20800	0.00780	included	HepG2	CSTF2
SE	SEC31A	ENSG00000138674.12	chr4	-	83783686	83783725	83778841	83778917	83784470	83784545	0.25200	0.00048	included	HepG2	CSTF2
SE	SNX14	ENSG00000135317.8	chr6	-	86248555	86248582	86246509	86246642	86251702	86251761	0.40400	0.00000	included	HepG2	CSTF2
SE	FN1	ENSG00000115414.14	chr2	-	216257653	216257926	216256354	216256537	216259250	216259481	0.05300	0.00756	included	HepG2	CSTF2
SE	CLSTN1	ENSG00000171603.12	chr1	-	9797555	9797612	9795942	9796100	9801151	9801314	0.21700	0.00373	included	HepG2	CSTF2
SE	RPS24	ENSG00000138326.14	chr10	+	79799961	79799983	79796951	79797062	79800372	79800455	0.27400	0.00000	included	HepG2	CSTF2
SE	BPIFB2	ENSG00000078898.6	chr20	+	31596005	31596184	31595405	31595566	31596346	31596489	-0.18200	0.00024	excluded	HepG2	CSTF2
SE	TFPI	ENSG00000003436.10	chr2	-	188378342	188378373	188368374	188368497	188394090	188394212	-0.18000	0.00633	excluded	HepG2	CSTF2
SE	RBCK1	ENSG00000125826.15	chr20	+	391055	391281	390473	390669	398169	398263	-0.20500	0.00042	excluded	HepG2	CSTF2

Showing 1 to 10 of 20 entries

2 RBPs can affect the splicing of CSTF2 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
SE	CSTF2	ENSG00000101811.9	chrX	+	100082564	100082736			100081673	100081746	100083028	100083091	0.08200	0.00000	0.00000	included	K562	AQR
SE	CSTF2	ENSG00000101811.9	chrX	+	100082564	100082736			100081673	100081746	100083028	100083091	0.08000	0.00121	0.04346	included	K562	PRPF8

Showing 1 to 2 of 2 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

CSTF2

General Information

Functions

Localization(RBPImage: CSTF2)

Domains (AlphaFold: CSTF2)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

CSTF2 protein has 2,738 RNA targets:

7 proteins are bound to CSTF2 RNA:

No autoregularoty interactions between protein CSTF2 and its RNA.

Diffrential Expression

5,011 differentially expressed genes after Knockdown/Knockout CSTF2 protein :

69 RBPs can affect the gene expression of CSTF2 :

20 alternative splicing events after Knockdown/Knockout CSTF2 protein :

2 RBPs can affect the splicing of CSTF2 :

No autoregularoty alternative splicing events between protein CSTF2 and its RNA.