ZNF574

General Information

Gene Name       : ZNF574
Gene ID             : ENSG00000105732.12
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: ZNF574)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ZNF574ENSG00000105732.120.753.692.22ENCSR000AEF
HepG2ZNF574ENSG00000105732.1210.562.946.75ENCSR985KAT
K562ZNF574ENSG00000105732.129.925.687.80ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq ZNF574-BGKcLV17-K562ENCSR064VKVZNF574K562ReleasedENCSR516TLX   CRISPRKD% = 64.5,79.9
knockdown followed by RNA-seq ZNF574-BGHcLV18-HepG2ENCSR821GQNZNF574HepG2ReleasedENCSR670KAS   CRISPRKD% = 96.2,95.6






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ZNF574ENCAB743ZXIPA5-54719UC2731463Released, ENCODE4BGHcLV18-53, Released, ENCODE4BGKcLV17-59, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ZNF574K562CRISPRENCSR064VKVENCSR516TLXZNF574-BGKcLV17-59,ZNF574-BGKcLV17-60NT-BGKcLV17-1,NT-BGKcLV17-264.5,79.9ReleasedENCODE4
ZNF574HepG2CRISPRENCSR821GQNENCSR670KASZNF574-BGHcLV18-53,ZNF574-BGHcLV18-54NT-BGHcLV18-1,NT-BGHcLV18-296.2,95.6ReleasedENCODE4
ZNF574Ribo-STAMP-293TshRNABGRSLV03-65,BGRSLV03-66BGRSLV03-1,BGRSLV03-2NotSatisfiedENCORE2
ZNF574Ribo-STAMP-293TshRNABGRSLV04-69,BGRSLV04-70BGRSLV04-1,BGRSLV04-2NotSatisfiedENCORE2
ZNF574Ribo-STAMP-293TshRNABGRSLV09-55,BGRSLV09-56BGRSLV09-1,BGRSLV09-2NotSatisfiedENCORE2
ZNF574Ribo-STAMP-293TshRNABGRSLV09-57,BGRSLV09-58BGRSLV09-1,BGRSLV09-2NotSatisfiedENCORE2
ZNF574Ribo-STAMP-293TshRNABGRSDLV04-61,BGRSDLV04-62BGRSDLV04-1,BGRSDLV04-2NotSatisfiedENCORE2
ZNF574Ribo-STAMP-293TshRNABGRSDLV04-63dox,BGRSDLV04-64doxBGRSDLV04-1,BGRSDLV04-2NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ZNF574Product_ID: PA5-54719
Lot_ID: UC2731463
Source: Thermo Fisher
Target Name: ZNF574-human		 ZNF574-HepG2-CRISPR-PA5-54719.png<br>Caption: Western blot following CRISPR against ZNF574 in HepG2 whole cell lysate using ZNF574 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ZNF574. ZNF574 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 ThermoFisher_PA5-54719_UC2731463_ZNF574.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ZNF574 specific antibody, PA5-54719. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the ZNF574-specific antibody, PA5-54719. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ZNF574-K562-CRISPR-PA5-54719.png<br>Caption: Western blot following CRISPR against ZNF574 in K562 whole cell lysate using ZNF574 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ZNF574. ZNF574 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for ZNF574 is not avaliable on ENCORE database.



Proteins bound to RNA
  • ZNF574 RNA is bound by 4 proteins.  







eCLIP assay for ZNF574 is not avaliable on ENCORE database.





4 proteins are bound to ZNF574 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr194258552842585565+3'-UTR5.156604.57427HepG2PCBP2
chr194258550742585578+3'-UTR6.8647435.61847K562PUM2
chr194258033942580501+5'-UTR,intron-Distal,intron-Proximal3.914943.45994HepG2NCBP2
chr194258421642584258+CDS4.464974.34093HepG2RBM15
chr194258360642583648+CDS4.144083.81597K562RBM15
chr194258460542584661+CDS3.034943.49427K562RBM15
chr194258421342584264+CDS4.997345.51215K562RBM15
chr194258288742582930+CDS4.721074.48994K562RBM15
chr194258447642584555+CDS3.650667.39702K562RBM15
chr194258325042583304+CDS4.865107.23707K562RBM15
chr194258515542585203+CDS3.272623.63681K562RBM15
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for ZNF574 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for ZNF574 is not available on ENCORE database.

  • 79 RBPs can affect the levels of ZNF574.  








The Knockdown/knockout RNA-Seq assay for ZNF574 is not available on ENCORE database.





79 RBPs can affect the gene expression of ZNF574 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
ZNF574ENSG00000105732.9276.48190165.038000.744305.125004.29810HepG2CELF1
ZNF574ENSG00000105732.9208.83170127.777500.708603.784402.97090HepG2DNAJC21
ZNF574ENSG00000105732.9186.81660126.644400.560702.731702.08370HepG2DNAJC2
ZNF574ENSG00000105732.9237.39800151.352400.649302.347101.49560HepG2EEF2
ZNF574ENSG00000105732.9172.73180109.832000.653102.765501.59860HepG2FAM120A
ZNF574ENSG00000105732.9183.15970109.371100.743803.138502.17900HepG2GEMIN5
ZNF574ENSG00000105732.9217.15020129.001600.751203.522302.80430HepG2HNRNPA1
ZNF574ENSG00000105732.9242.60820143.488800.757601.936001.34410HepG2HNRNPC
ZNF574ENSG00000105732.9318.82930240.243900.408202.153001.51400HepG2HNRNPF
ZNF574ENSG00000105732.9241.58860161.656700.579503.239202.51440HepG2HNRNPL
ZNF574ENSG00000105732.9380.08740226.880500.744303.866203.08040HepG2IGF2BP2
ZNF574ENSG00000105732.9179.33240109.949300.705702.490201.41830HepG2LARP7
ZNF574ENSG00000105732.9383.50750240.872900.670902.834402.17180HepG2MAGOH
ZNF574ENSG00000105732.9388.08310247.858000.646802.523401.91680HepG2NCBP2
ZNF574ENSG00000105732.9264.52940175.971200.588003.270602.44370HepG2NOL12
ZNF574ENSG00000105732.9258.24210130.525000.984302.332701.33700HepG2NONO
ZNF574ENSG00000105732.9259.97780167.978200.630002.787102.18860HepG2PUF60
ZNF574ENSG00000105732.9320.47950178.314200.845707.839406.94720HepG2PUM1
ZNF574ENSG00000105732.9365.13710178.185301.035009.592308.38240HepG2PUM2
ZNF574ENSG00000105732.9345.46800243.380000.505203.470302.79820HepG2PUS1
ZNF574ENSG00000105732.9409.66540287.889100.508803.488602.92550HepG2RBM22
ZNF574ENSG00000105732.9268.31660132.590401.016905.484404.07480HepG2RBM27
ZNF574ENSG00000105732.9193.90280107.670100.848602.463001.46340HepG2RCC2
ZNF574ENSG00000105732.9201.08070119.553000.750003.389902.71790HepG2RPS19
ZNF574ENSG00000105732.9209.57360122.267100.777303.012302.17760HepG2RPS2
ZNF574ENSG00000105732.9516.94240358.817800.526702.692402.02770HepG2SF3A3
ZNF574ENSG00000105732.9245.74810161.060800.609503.537902.76090HepG2SLBP
ZNF574ENSG00000105732.9215.41110148.034300.541102.660602.02690HepG2SMN1
ZNF574ENSG00000105732.9330.24430165.851600.993509.548008.47930HepG2SRSF5
ZNF574ENSG00000105732.9120.68280195.05720-0.692702.380401.86100HepG2SRSF9
ZNF574ENSG00000105732.9323.28060188.621000.777206.263005.39640HepG2SUCLG1
ZNF574ENSG00000105732.9203.38340138.510500.554102.417501.94470HepG2TIAL1
ZNF574ENSG00000105732.9221.83520139.742300.666603.893603.24790HepG2TRIM56
ZNF574ENSG00000105732.9299.60070164.104300.868303.817202.77490HepG2TUFM
ZNF574ENSG00000105732.9541.92110275.537100.975708.902207.76850HepG2U2AF1
ZNF574ENSG00000105732.9409.59600147.404501.474304.915903.57360HepG2U2AF2
ZNF574ENSG00000105732.9435.27250307.225800.502503.784403.10110HepG2UTP3
ZNF574ENSG00000105732.9250.98170139.107500.851302.176301.52730HepG2XRN2
ZNF574ENSG00000105732.9183.36900244.26690-0.413701.899801.32910HepG2ZC3H8
ZNF574ENSG00000105732.9563.23170356.816700.658407.155806.30660K562AGGF1
ZNF574ENSG00000105732.9530.35170392.102000.435602.953701.92770K562AKAP8
ZNF574ENSG00000105732.9523.27880371.287500.494903.482002.74610K562AKAP8L
ZNF574ENSG00000105732.93691.88530463.335102.99410101.1371098.16400K562AQR
ZNF574ENSG00000105732.9532.99770389.576700.452103.712702.64160K562ATP5C1
ZNF574ENSG00000105732.9460.20770333.290100.465402.945501.85210K562CCAR1
ZNF574ENSG00000105732.9743.79010535.137100.474903.004801.49450K562CKAP4
ZNF574ENSG00000105732.9825.77980590.366300.484002.830701.71030K562CPEB4
ZNF574ENSG00000105732.9576.30070418.643900.461003.200201.62330K562DDX19B
ZNF574ENSG00000105732.9831.76770611.021600.444904.841304.01350K562DDX47
ZNF574ENSG00000105732.9554.62290399.509700.473204.201303.09060K562DKC1
ZNF574ENSG00000105732.9547.49480339.003300.691403.362102.24390K562EIF3A
ZNF574ENSG00000105732.9215.58250120.782800.835704.725703.69880K562EIF3G
ZNF574ENSG00000105732.9560.90440299.366900.905704.431803.39260K562EIF4A3
ZNF574ENSG00000105732.9412.29320269.612600.612704.208902.73290K562EIF4B
ZNF574ENSG00000105732.9914.76940659.737000.471404.680803.47130K562EIF4G2
ZNF574ENSG00000105732.9650.97590457.836300.507704.324802.69590K562EXOSC5
ZNF574ENSG00000105732.9506.74760311.332000.702707.258106.34110K562FXR1
ZNF574ENSG00000105732.924.8304072.47600-1.545404.446503.50620K562G3BP2
ZNF574ENSG00000105732.9605.62890461.383100.392402.952601.77380K562GLRX3
ZNF574ENSG00000105732.9765.04370552.159900.470403.991602.69490K562HNRNPL
ZNF574ENSG00000105732.9426.52630571.48450-0.422102.552101.78520K562HSPD1
ZNF574ENSG00000105732.9351.54570152.864501.201403.230302.09060K562IGF2BP2
ZNF574ENSG00000105732.9256.07210155.422700.720303.824302.37910K562IGF2BP3
ZNF574ENSG00000105732.9344.22180160.503101.100605.957604.70660K562MAGOH
ZNF574ENSG00000105732.9598.76100441.399600.439803.347902.33090K562MBNL1
ZNF574ENSG00000105732.9527.79960393.833400.422302.218201.43450K562METAP2
ZNF574ENSG00000105732.9625.18350485.075000.366002.859802.03670K562MTPAP
ZNF574ENSG00000105732.9412.62150221.714200.896002.841401.52390K562NONO
ZNF574ENSG00000105732.9696.93950539.013400.370603.537002.70410K562NSUN2
ZNF574ENSG00000105732.9433.81170261.892200.728002.628001.66940K562PABPN1
ZNF574ENSG00000105732.9396.50920218.500200.859602.646401.91270K562PRPF8
ZNF574ENSG00000105732.9442.48730307.066900.527002.893101.96550K562PSIP1
ZNF574ENSG00000105732.91026.62140750.158200.452504.618903.36840K562PUM2
ZNF574ENSG00000105732.9520.43940368.189900.499203.409302.81570K562PUS1
ZNF574ENSG00000105732.9465.68310358.766700.376202.401801.84240K562RBM15
ZNF574ENSG00000105732.9279.54800149.454900.903306.426405.07530K562RBM22
ZNF574ENSG00000105732.9584.97280398.078600.555202.853301.75390K562SAFB
ZNF574ENSG00000105732.9789.07950531.792000.569206.556105.34750K562SF3A3
ZNF574ENSG00000105732.9167.0344075.367601.148006.927405.53630K562SF3B4
ZNF574ENSG00000105732.9573.23850291.925500.973402.964101.59410K562SFPQ
ZNF574ENSG00000105732.9409.42260265.648300.624004.025902.71630K562SRFBP1
ZNF574ENSG00000105732.9670.07970544.825400.298402.248601.39570K562SRPK2
ZNF574ENSG00000105732.9289.98770152.900100.923306.572505.49310K562SRSF1
ZNF574ENSG00000105732.9457.04190240.819900.924308.083306.14980K562SRSF4
ZNF574ENSG00000105732.9237.70020144.444700.718503.945602.65490K562SRSF5
ZNF574ENSG00000105732.9326.44210154.708301.077208.320407.00680K562TARDBP
ZNF574ENSG00000105732.9286.99790149.243200.943304.104103.07860K562TIA1
ZNF574ENSG00000105732.9273.27290146.538700.899005.905504.16570K562TRA2A
ZNF574ENSG00000105732.9476.76720313.844300.603103.137402.11730K562TRIM56
ZNF574ENSG00000105732.9413.46460228.054200.858302.704401.51500K562XRN2
ZNF574ENSG00000105732.9707.46900521.940900.438703.313202.35810K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for ZNF574 is not available on ENCORE database.






No RBPs affect the alternative splcing events of ZNF574 on ENCORE database.





The Knockdown/knockout RNA-Seq assay for ZNF574 is not avaliable on ENCORE database.