SMG9

General Information

Gene Name       : SMG9
Gene ID             : ENSG00000105771.13
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: SMG9)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878SMG9ENSG00000105771.1313.8517.9815.91ENCSR000AEF
HepG2SMG9ENSG00000105771.1322.0316.6619.34ENCSR985KAT
K562SMG9ENSG00000105771.1344.0536.0840.06ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq SMG9-BGKcLV33-K562SMG9K562Released   CRISPRKD% = 77.8,71.8
knockdown followed by RNA-seq SMG9-BGKcLV33-K562ENCSR971FJUSMG9K562ReleasedENCSR271ZAB   CRISPRKD% = band isn't on the position,






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
SMG9ENCAB220KQWA302-210A1BGKcLV33-61, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
SMG9K562CRISPRBGKcLV33-57,BGKcLV33-58BGKcLV33-1,BGKcLV33-277.8,71.8ReleasedENCODE4
SMG9K562CRISPRENCSR971FJUENCSR271ZABSMG9-BGKcLV33-61,SMG9-BGKcLV33-62NT-BGKcLV33-1,NT-BGKcLV33-2band isn't on the position,ReleasedENCODE4
SMG9HepG2CRISPRBGHcLV31-57,BGHcLV31-58BGHcLV31-1,BGHcLV31-2NO CLEAR BAND,NotSatisfiedENCODE4
SMG9HepG2CRISPRBGHcLV34-53,BGHcLV34-54BGHcLV34-1,BGHcLV34-2NO CLEAR BAND ON THE POSITION,NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMG9Product_ID: A302-210A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SMG9-human		 SMG9-K562-CRISPR-A302-210A.png<br>Caption: Western blot following CRISPR against SMG9 in K562 whole cell lysate using SMG9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG9. SMG9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for SMG9 is not avaliable on ENCORE database.



Proteins bound to RNA
  • SMG9 RNA is bound by 13 proteins.  







eCLIP assay for SMG9 is not avaliable on ENCORE database.





13 proteins are bound to SMG9 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr194424177344241882-3'-UTR,CDS,intron-Distal,intron-Proximal3.238877.24530K562BUD13
chr194423551644235743-3'-UTR,intron-Distal,intron-Proximal4.558825.58993HepG2PABPN1
chr194423544344235556-3'-UTR,intron-Distal,intron-Proximal3.840553.44763HepG2LSM11
chr194424229544242372-3'-UTR,CDS,intron-Distal,intron-Proximal3.242564.25541HepG2GRWD1
chr194424182644241894-3'-UTR,CDS,intron-Distal,intron-Proximal3.604396.26686K562SF3B4
chr194423560244235708-3'-UTR,intron-Distal3.598478.82905K562UPF1
chr194423547244235602-3'-UTR,intron-Distal,intron-Proximal4.7236814.74404K562UPF1
chr194423550044235555-3'-UTR,intron-Distal,intron-Proximal3.745823.19539K562YBX3
chr194423534944235457-3'-UTR,intron-Distal,intron-Proximal3.603117.91779K562UPF1
chr194423546344235703-3'-UTR,intron-Distal,intron-Proximal5.023017.94456HepG2UPF1
chr194425155344251603-CDS,intron-Distal,intron-Proximal3.088364.78164HepG2PRPF8
chr194423704244237116-CDS,intron-Distal3.551124.52024HepG2PPIG
chr194423700944237136-CDS,intron-Distal,intron-Proximal4.140573.89860HepG2PABPN1
chr194423748944237546-CDS,intron-Distal,intron-Proximal3.308413.64266HepG2PPIG
chr194423777444237831-CDS,intron-Distal,intron-Proximal3.347073.12941K562UCHL5
chr194425200144252054-CDS,intron-Distal,intron-Proximal4.154285.20093K562DDX24
chr194423777144237806-CDS,intron-Distal,intron-Proximal3.176893.05161HepG2PPIG
chr194425212844252178-CDS,intron-Distal,intron-Proximal3.393963.32039HepG2PPIG
chr194424433144244388-CDS,intron-Distal,intron-Proximal3.644364.77019K562BUD13
chr194423834344238388-intron-Distal5.169336.03057K562EFTUD2
chr194424089744241053-intron-Distal3.232123.86715K562HNRNPK
chr194423838944238493-intron-Distal,intron-Proximal6.2013913.01998K562EFTUD2
chr194424167944241737-intron-Distal,intron-Proximal4.274444.46002K562DDX24
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for SMG9 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for SMG9 is not available on ENCORE database.

  • 79 RBPs can affect the levels of SMG9.  








The Knockdown/knockout RNA-Seq assay for SMG9 is not available on ENCORE database.





79 RBPs can affect the gene expression of SMG9 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
SMG9ENSG00000105771.9812.95150612.534800.408302.133601.30340HepG2ABCF1
SMG9ENSG00000105771.9616.64520427.499000.528401.918501.46610HepG2AKAP1
SMG9ENSG00000105771.9678.01940532.962900.347202.746502.06370HepG2AUH
SMG9ENSG00000105771.9824.38740629.277000.389502.782402.18270HepG2BOP1
SMG9ENSG00000105771.9536.85750417.968400.361002.896502.22010HepG2CKAP4
SMG9ENSG00000105771.9282.56940187.876500.588702.767101.68890HepG2CSTF2
SMG9ENSG00000105771.9379.59280262.300500.533102.566901.67640HepG2EEF2
SMG9ENSG00000105771.9494.71580338.113200.549003.225902.58800HepG2EIF2S1
SMG9ENSG00000105771.9758.19250373.578701.0211010.441009.21920HepG2EIF4A3
SMG9ENSG00000105771.9364.83910189.571800.944407.072405.65230HepG2GEMIN5
SMG9ENSG00000105771.9421.75760270.152000.642503.905002.69230HepG2GNB2L1
SMG9ENSG00000105771.9395.58680654.93890-0.727404.744903.92110HepG2GPKOW
SMG9ENSG00000105771.9425.10480288.891300.557203.393702.68710HepG2HNRNPA1
SMG9ENSG00000105771.9615.48290321.342100.937509.820408.58030HepG2HNRNPC
SMG9ENSG00000105771.9596.69200354.432200.751406.355605.32780HepG2HNRNPK
SMG9ENSG00000105771.9457.10250319.217300.517904.516303.75790HepG2HNRNPU
SMG9ENSG00000105771.9601.69060355.466000.759206.299805.31840HepG2IGF2BP2
SMG9ENSG00000105771.9986.23230553.921200.832107.326105.96150HepG2LARP4
SMG9ENSG00000105771.9605.10760377.288200.681403.838603.06680HepG2MAGOH
SMG9ENSG00000105771.9364.42780554.16640-0.604704.032503.13360HepG2NSUN2
SMG9ENSG00000105771.9460.57710352.063600.387502.381201.80940HepG2PCBP1
SMG9ENSG00000105771.9621.37010447.985000.471904.419703.46770HepG2PRPF4
SMG9ENSG00000105771.9671.71960467.974000.521304.731203.86540HepG2PUM2
SMG9ENSG00000105771.9485.31850387.939300.323001.943301.40510HepG2QKI
SMG9ENSG00000105771.9386.06970561.83880-0.541304.664403.58640HepG2RBM15
SMG9ENSG00000105771.9713.13830511.473600.479404.446503.83020HepG2RBM22
SMG9ENSG00000105771.9448.06330352.111500.347601.888401.32160HepG2RBM25
SMG9ENSG00000105771.9817.91650534.130700.614706.125305.16560HepG2RPLP0
SMG9ENSG00000105771.9396.40080207.190700.935908.186807.19550HepG2RPS19
SMG9ENSG00000105771.9895.34410557.461000.683504.667603.77800HepG2SF3A3
SMG9ENSG00000105771.9499.98300688.36670-0.461302.011701.35010HepG2SNRNP70
SMG9ENSG00000105771.91010.98860577.674300.8073015.4854014.55400HepG2SRFBP1
SMG9ENSG00000105771.9599.34110487.197900.298802.421101.76690HepG2SRSF7
SMG9ENSG00000105771.9603.30450501.529500.266501.745601.31540HepG2SSB
SMG9ENSG00000105771.9595.30850495.380900.265001.833201.30800HepG2SUCLG1
SMG9ENSG00000105771.9532.92010310.164400.780803.075602.55290HepG2TIAL1
SMG9ENSG00000105771.9716.98170529.046500.438402.638802.02420HepG2U2AF1
SMG9ENSG00000105771.91276.95000438.392201.542306.376604.80140HepG2U2AF2
SMG9ENSG00000105771.9482.48600272.593400.823604.412903.12540HepG2UPF2
SMG9ENSG00000105771.9926.97220477.308100.9575019.7821018.39790HepG2UTP3
SMG9ENSG00000105771.92349.817501836.908800.355205.755104.98460K562AGGF1
SMG9ENSG00000105771.92462.983501911.379800.365702.699702.05440K562AKAP8L
SMG9ENSG00000105771.91168.971201429.17960-0.289902.159801.63000K562AQR
SMG9ENSG00000105771.92425.019302005.484100.273903.056602.09670K562ATP5C1
SMG9ENSG00000105771.91106.17510904.544400.290202.860301.88410K562BCLAF1
SMG9ENSG00000105771.91303.84310946.516200.462002.892201.81090K562CCAR1
SMG9ENSG00000105771.91416.146401781.81920-0.331403.251902.39990K562CPSF6
SMG9ENSG00000105771.92239.813201814.183100.304004.089403.31850K562DDX47
SMG9ENSG00000105771.9642.17810482.657500.411903.323002.45530K562EIF3G
SMG9ENSG00000105771.91139.40770882.719700.368203.527002.04020K562EWSR1
SMG9ENSG00000105771.91123.088501536.80740-0.452503.857302.76210K562FTO
SMG9ENSG00000105771.9273.82610556.61870-1.023408.613507.22230K562G3BP2
SMG9ENSG00000105771.91210.86770827.451700.549204.468002.62370K562GEMIN5
SMG9ENSG00000105771.91203.576901701.14690-0.499205.626004.49780K562GPKOW
SMG9ENSG00000105771.91664.107801357.484800.293702.638301.59010K562HNRNPA1
SMG9ENSG00000105771.91666.107001092.146000.609208.434607.44310K562HNRNPC
SMG9ENSG00000105771.91351.447701639.47940-0.278702.255501.31080K562HNRNPL
SMG9ENSG00000105771.91767.587701503.410500.233402.189901.39620K562HNRNPU
SMG9ENSG00000105771.91690.999502067.01050-0.289702.215001.50060K562HSPD1
SMG9ENSG00000105771.91186.93780768.275700.627405.214903.62390K562IGF2BP2
SMG9ENSG00000105771.91483.300801107.751800.421103.301701.66850K562LIN28B
SMG9ENSG00000105771.92766.382202272.402500.283702.507401.64960K562MBNL1
SMG9ENSG00000105771.91248.800601660.53120-0.411103.980702.91770K562METAP2
SMG9ENSG00000105771.91634.516202232.03080-0.449504.832303.33720K562MSI2
SMG9ENSG00000105771.91455.762302203.93360-0.598303.248501.89550K562PABPC1
SMG9ENSG00000105771.9842.031601724.31340-1.0341015.7379013.80240K562PCBP3
SMG9ENSG00000105771.91384.454001066.297900.376603.372301.77510K562PHF6
SMG9ENSG00000105771.9623.974701322.59330-1.0838023.3193021.16090K562POLR2G
SMG9ENSG00000105771.91374.99430979.232100.489605.033703.76550K562PSIP1
SMG9ENSG00000105771.91037.97950784.095600.404603.772203.00790K562PTBP1
SMG9ENSG00000105771.91892.206202284.74520-0.272002.122501.40830K562PUF60
SMG9ENSG00000105771.91860.611902430.11100-0.385204.637103.22720K562PUM1
SMG9ENSG00000105771.92513.491201895.615000.406904.197603.54980K562PUS1
SMG9ENSG00000105771.92187.115801846.949200.243802.397501.83890K562RBM15
SMG9ENSG00000105771.9588.65330817.84140-0.474402.183401.46180K562RPS19
SMG9ENSG00000105771.91582.030001178.506100.424704.341002.97510K562SAFB
SMG9ENSG00000105771.91260.13200846.976300.573106.184604.48600K562SRFBP1
SMG9ENSG00000105771.91884.695401641.048800.199602.267901.41180K562SRPK2
SMG9ENSG00000105771.9529.78080768.72160-0.537104.768103.84450K562SRSF1
SMG9ENSG00000105771.91030.45300726.114500.504904.628603.20840K562SRSF5
SMG9ENSG00000105771.91724.565701326.614700.378404.248103.22440K562SYNCRIP
SMG9ENSG00000105771.91024.72440750.395200.449404.300003.24630K562TIA1
SMG9ENSG00000105771.91196.00150941.852400.344502.224601.39340K562TIAL1
SMG9ENSG00000105771.9967.03680736.597600.392602.921201.79200K562TRA2A
SMG9ENSG00000105771.91454.731201000.643600.539706.105204.57530K562TRIM56
SMG9ENSG00000105771.92350.106102016.992700.220402.693501.44220K562TROVE2
SMG9ENSG00000105771.92007.656601721.279200.221902.526401.83870K562UPF1
SMG9ENSG00000105771.91829.907801472.849900.313102.812401.92880K562WDR3
SMG9ENSG00000105771.91776.625701531.554200.214002.452901.65370K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for SMG9 is not available on ENCORE database.





10 RBPs can affect the splicing of SMG9 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SESMG9ENSG00000105771.9chr19-442489784424903644243824442443704425159344251691-0.579000.005530.08197excludedHepG2U2AF1
SESMG9ENSG00000105771.9chr19-442489234424903644244258442443704425159344251691-0.248000.000260.00893excludedHepG2HNRNPK
SESMG9ENSG00000105771.9chr19-442489234424903644244258442443704425159344251691-0.058000.000380.00618excludedHepG2U2AF2
SESMG9ENSG00000105771.9chr19-442489234424903644244258442443704425159344251691-0.105000.001180.01958excludedK562PUS1
SESMG9ENSG00000105771.9chr19-442489234424903644244258442443704425159344251691-0.081000.001910.05518excludedK562UTP18
SESMG9ENSG00000105771.9chr19-442489234424903644244258442443704425159344251691-0.083000.001300.03334excludedK562RBM15
SESMG9ENSG00000105771.9chr19-442489234424903644244258442443704425159344251691-0.081000.001820.04396excludedK562AGGF1
A3SSSMG9ENSG00000105771.9chr19-4423854044238740442385404423864744241748442418340.100000.000000.00015longerK562NPM1
A3SSSMG9ENSG00000105771.9chr19-4423854044238740442385404423864744241748442418340.115000.000310.01012longerHepG2U2AF2
A3SSSMG9ENSG00000105771.9chr19-442385404423874044238540442386474424174844241834-0.076000.000070.05525shorterK562EIF4G2
A3SSSMG9ENSG00000105771.9chr19-4423747544237626442374754423760444237777442378850.053000.000320.00252longerK562AQR
A3SSSMG9ENSG00000105771.9chr19-4423777744237908442377774423788544238540442386470.090000.000030.00031longerK562AQR
A3SSSMG9ENSG00000105771.9chr19-4423854044238740442385404423864744241748442418340.159000.000000.00000longerK562PUS1
A3SSSMG9ENSG00000105771.9chr19-4423854044238740442385404423864744241748442418340.094000.000530.07292longerK562UTP18
A3SSSMG9ENSG00000105771.9chr19-4423777744237930442377774423788544238540442389770.096000.001180.04606longerHepG2U2AF1
A3SSSMG9ENSG00000105771.9chr19-4423854044238740442385404423864744241748442418340.263000.000000.00000longerHepG2U2AF1
A3SSSMG9ENSG00000105771.9chr19-4423747544237626442374754423760444237777442378850.131000.000030.00266longerK562U2AF1
A3SSSMG9ENSG00000105771.9chr19-4423854044238740442385404423864744241748442417770.143000.000000.00003longerK562RBM15

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for SMG9 is not avaliable on ENCORE database.