SMG9      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: Sequenced)
BGHcLV49-101BGHcLV49-101BGHcLV49-102BGHcLV49-102
idx0000
TRCN#_or_BGC#BGC#0000876BGC#0000876BGC#0000876BGC#0000876
shRNA_or_gRNA_sequenceATGGGATAGAGCGGCGGCGAATGGGATAGAGCGGCGGCGAATGGGATAGAGCGGCGGCGAATGGGATAGAGCGGCGGCGA
PAMCGGCGGCGGCGG
NameSMG9_91SMG9_91SMG9_91SMG9_91
Sample_IDBGHcLV49-101BGHcLV49-101BGHcLV49-102BGHcLV49-102
transduction_Date6/6/256/6/256/6/256/6/25
daysD6D6D6D6
RBP_nameSMG9SMG9SMG9SMG9
qPCR_result17.00.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc4694469450995099
WB_result94.583.3
Ave_WB
WB_DONE_date7/3/257/21/25no band on the posio
MW58kd58kd
IP
antibody_Cat#A302-210AA302-211ALOT #1LOT #1
Antibody DCC IDENCAB220KQW
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID9987998999889990




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMG9Product_ID: A302-210A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SMG9-human		 SMG9-hepg2-CRISPR-A302-210A-LICOR.png<br>Caption: Western blot following CRISPR against SMG9 in HepG2 whole cell lysate using SMG9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG9. SMG9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SMG9-K562-CRISPR-A302-210A.png<br>Caption: Western blot following CRISPR against SMG9 in K562 whole cell lysate using SMG9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG9. SMG9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-101BGHcLV49-101BGHcLV49-102BGHcLV49-102
Sample_IDBGHcLV49-101BGHcLV49-101BGHcLV49-102BGHcLV49-102
Sample Name
Sample NameSMG9-BGHcLV49-101SMG9-BGHcLV49-102
RBPSMG9SMG9SMG9SMG9
Cell_LineHepG2HepG2HepG2HepG2
Exp UID
StatusSequencedNotSatisfiedSequencedNotSatisfied
Status_date2025-10-302025-08-122025-10-302025-08-12
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14366144361436714437




Library-Prep Information
BGHcLV49-101BGHcLV49-102
Sample #6364
Sample NameSMG9-BGHcLV49-101SMG9-BGHcLV49-102
Sample_Name_Alias
Index Well PositionA09B09
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp269262
Peak_Molarity68.5079.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-101BGHcLV49-102
RBPSMG9SMG9
Batch_IDBGHcLV49BGHcLV49
WB_result94.50083.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48084809




Sequencing Information
BGHcLV49-101BGHcLV49-102
Sample_IDBGHcLV49-101BGHcLV49-102
Sample NameSMG9-BGHcLV49-101SMG9-BGHcLV49-102
Pool IDPool-250916Pool-250916
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID35333534




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database