SMG9      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-101BGHcLV49-101BGHcLV49-102BGHcLV49-102
idx0000
TRCN#_or_BGC#BGC#0000876BGC#0000876BGC#0000876BGC#0000876
shRNA_or_gRNA_sequenceATGGGATAGAGCGGCGGCGAATGGGATAGAGCGGCGGCGAATGGGATAGAGCGGCGGCGAATGGGATAGAGCGGCGGCGA
PAMCGGCGGCGGCGG
NameSMG9_91SMG9_91SMG9_91SMG9_91
Sample_IDBGHcLV49-101BGHcLV49-101BGHcLV49-102BGHcLV49-102
transduction_Date6/6/256/6/256/6/256/6/25
daysD6D6D6D6
RBP_nameSMG9SMG9SMG9SMG9
qPCR_result17.00.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc4694469450995099
WB_result94.583.3
Ave_WB
WB_DONE_date7/3/257/21/25no band on the posio
MW58kd58kd
IP
antibody_Cat#A302-210AA302-211ALOT #1LOT #1
Antibody DCC IDENCAB220KQW
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID9985998799869988




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMG9Product_ID: A302-210A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SMG9-human		 SMG9-hepg2-CRISPR-A302-210A-LICOR.png<br>Caption: Western blot following CRISPR against SMG9 in HepG2 whole cell lysate using SMG9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG9. SMG9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SMG9-K562-CRISPR-A302-210A.png<br>Caption: Western blot following CRISPR against SMG9 in K562 whole cell lysate using SMG9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG9. SMG9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-101BGHcLV49-101BGHcLV49-102BGHcLV49-102
Sample_IDBGHcLV49-101BGHcLV49-101BGHcLV49-102BGHcLV49-102
Sample Name
Sample Name
RBPSMG9SMG9SMG9SMG9
Cell_LineHepG2HepG2HepG2HepG2
Exp UID
StatusLibPrepingNotSatisfiedLibPrepingNotSatisfied
Status_date2025-08-122025-08-122025-08-122025-08-12
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14366144361436714437




Library-Prep Information
BGHcLV49-101BGHcLV49-102
Sample #6364
Sample NameSMG9-BGHcLV49-101SMG9-BGHcLV49-102
Sample_Name_Alias
Index Well PositionA09B09
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp269262
Peak_Molarity68.5079.70
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-101BGHcLV49-102
RBPSMG9SMG9
Batch_IDBGHcLV49BGHcLV49
WB_result94.50083.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48084809




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database