AIMP1

General Information

Gene Name       : AIMP1
Gene ID             : ENSG00000164022.16
Essential Gene : No

Functions

Localization(RBPImage: AIMP1)

Domains (AlphaFold: AIMP1)


Antibody Validation

 View validation images for all AIMP1 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878AIMP1ENSG00000164022.1662.8945.4654.17ENCSR000AEF
HepG2AIMP1ENSG00000164022.1638.4387.4562.94ENCSR985KAT
K562AIMP1ENSG00000164022.1653.7159.9156.81ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq AIMP1-BGKcLV26-K562ENCSR929YETAIMP1K562ReleasedENCSR900GQL   CRISPRKD% = 62.6,57.917303
knockdown followed by RNA-seq AIMP1-BGHcLV27-HepG2ENCSR512BITAIMP1HepG2ReleasedENCSR650TTU   CRISPRKD% = 68.7,75.5






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
AIMP1ENCAB938MRHA304-895A1Released, ENCODE4BGHcLV27-3, Released, ENCODE4BGKcLV26-19, Released, ENCODE4
AIMP1ENCAB034EJCA304-896A1Released, ENCODE4BGHcLV27-3, Released, ENCODE4BGKcLV26-19, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
AIMP1K562CRISPRENCSR929YETENCSR900GQLAIMP1-BGKcLV26-19,AIMP1-BGKcLV26-20NT-BGKcLV26-1,NT-BGKcLV26-262.6,57.917303ReleasedENCODE4
AIMP1HepG2CRISPRENCSR512BITENCSR650TTUAIMP1-BGHcLV27-3,AIMP1-BGHcLV27-4NT-BGHcLV27-1,NT-BGHcLV27-268.7,75.5ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  
    AIMP1: Click to RBP Image Database
  • Cytoplasm
  • Compartment/Organelle
  • Unknown Cyto Foci
  • Cytosolic












    • RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
    AIMP1Product_ID: A304-896A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: AIMP1-human    		 Bethyl_A304-896A_1_AIMP1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the AIMP1 specific antibody, A304-896A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the AIMP1-specific antibody, A304-896A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
    		 AIMP1-K562-CRISPR-A304-896A.png<br>Caption: Western blot following CRISPR against AIMP1 in K562 whole cell lysate using AIMP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AIMP1. AIMP1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
    AIMP1Product_ID: A304-895A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: AIMP1-human    		 AIMP1-HEPG2-CRISPR-A304-895A.png<br>Caption: Western blot following CRISPR against AIMP1 in HepG2 whole cell lysate using AIMP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AIMP1. AIMP1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
    		 Bethyl_A304-895A_1_AIMP1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the AIMP1 specific antibody, A304-895A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the AIMP1-specific antibody, A304-895A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
    		 AIMP1-K562-CRISPR-A304-895A.png<br>Caption: Western blot following CRISPR against AIMP1 in K562 whole cell lysate using AIMP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AIMP1. AIMP1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.








    RNA Targets

    • eCLIP assay for AIMP1 is not avaliable on ENCORE database.



    Proteins bound to RNA
    • AIMP1 RNA is bound by 21 proteins.  







    eCLIP assay for AIMP1 is not avaliable on ENCORE database.





    21 proteins are bound to AIMP1 RNA:

    Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
    chr4107246141107246174+3'-UTR,5'-UTR,CDS,intron-Proximal3.678715.63105K562DDX3X
    chr4107270029107270169+3'-UTR3.626995.13388HepG2HNRNPL
    chr4107246140107246173+3'-UTR,5'-UTR,CDS,intron-Proximal4.558665.82083HepG2GRWD1
    chr4107270039107270173+3'-UTR3.218144.95846K562HNRNPL
    chr4107252958107253044+CDS,intron-Proximal3.1863410.49889HepG2BCLAF1
    chr4107268682107268713+CDS,intron-Proximal4.296434.69071HepG2GRWD1
    chr4107249299107249371+CDS3.668136.18214HepG2GRWD1
    chr4107258025107258031+CDS,intron-Proximal3.485904.20387K562ZNF622
    chr4107248613107248688+CDS3.224426.35635K562UCHL5
    chr4107258047107258101+CDS3.051115.41277K562UCHL5
    chr4107246174107246245+CDS3.4265611.03951K562DDX3X
    chr4107268672107268738+CDS,intron-Proximal3.335203.50696K562BUD13
    chr4107249232107249241+CDS,intron-Proximal4.310874.16170K562UCHL5
    chr4107248607107248703+CDS,intron-Proximal3.9022714.30125HepG2BCLAF1
    chr4107258047107258138+CDS3.7708510.01989K562ZNF622
    chr4107237750107237758+CDS,intron-Distal,intron-Proximal4.9815110.08262K562AQR
    chr4107246190107246235+CDS4.568365.44793K562ZNF622
    chr4107248706107248722+CDS,intron-Proximal4.524295.79022K562YBX3
    chr4107252826107252919+CDS,intron-Proximal3.039776.97433K562ZNF800
    chr4107249231107249299+CDS,intron-Proximal4.4888315.11988HepG2BCLAF1
    chr4107248609107248724+CDS,intron-Proximal3.1836713.73403K562ZNF622
    chr4107268714107268826+CDS3.685356.81726HepG2GRWD1
    chr4107253007107253040+CDS4.063983.27187HepG2ZNF800
    chr4107258131107258188+CDS3.308413.64266HepG2PPIG
    chr4107249241107249323+CDS3.117367.74926K562UCHL5
    chr4107258101107258168+CDS3.657724.41784HepG2GRWD1
    chr4107252828107252951+CDS,intron-Proximal4.3108716.33279K562UCHL5
    chr4107248703107248722+CDS,intron-Proximal3.027996.06438HepG2BCLAF1
    chr4107248617107248724+CDS,intron-Proximal3.468266.70305K562ZNF800
    chr4107249379107249402+CDS,intron-Proximal3.509496.44869K562UCHL5
    chr4107237715107237805+CDS,intron-Distal,intron-Proximal3.885778.18981HepG2PRPF8
    chr4107252828107252872+CDS,intron-Proximal3.857396.81385HepG2BCLAF1
    chr4107252944107253039+CDS3.9214310.87852HepG2GRWD1
    chr4107249295107249401+CDS,intron-Proximal3.771154.49016HepG2FXR2
    chr4107252798107252930+CDS,intron-Proximal3.303767.92183HepG2BUD13
    chr4107249299107249384+CDS3.761629.83905HepG2BCLAF1
    chr4107258178107258194+CDS3.876013.56019HepG2GRWD1
    chr4107252828107252922+CDS,intron-Proximal3.399064.76953K562DDX24
    chr4107252953107253043+CDS,intron-Proximal3.904923.11282HepG2G3BP1
    chr4107249279107249399+CDS4.350734.67790K562CPSF6
    chr4107258101107258179+CDS3.395835.71803K562UCHL5
    chr4107252854107252967+CDS3.141315.94696HepG2ZNF800
    chr4107268682107268748+CDS,intron-Proximal4.590124.97342HepG2PPIG
    chr4107252888107252958+CDS4.6056510.68105HepG2BCLAF1
    chr4107252910107253041+CDS,intron-Proximal3.482207.20571K562PUM1
    chr4107252973107253030+CDS3.351243.05368K562ZNF800
    chr4107237726107237790+CDS,intron-Distal,intron-Proximal3.402243.90373HepG2AQR
    chr4107252961107253042+CDS,intron-Proximal4.1459612.81426K562ZNF622
    chr4107268780107268831+CDS3.946244.09885HepG2BCLAF1
    chr4107268771107268834+CDS4.789868.09261K562LIN28B
    chr4107246178107246260+CDS3.651555.97027HepG2GRWD1
    chr4107249229107249336+CDS,intron-Proximal4.3622815.36777K562YBX3
    chr4107237712107237768+CDS,intron-Distal,intron-Proximal3.119314.55250HepG2GRWD1
    chr4107241894107241945+intron-Distal3.905876.57821HepG2AQR
    chr4107263904107263972+intron-Distal3.900325.80937K562EXOSC5
    chr4107261779107261842+intron-Distal4.373093.30687K562EXOSC5
    chr4107240945107241011+intron-Distal3.327013.81210HepG2KHSRP
    chr4107237501107237599+intron-Distal,intron-Proximal3.890015.56912HepG2AQR
    chr4107237799107237840+intron-Distal,intron-Proximal3.445565.28960K562AQR
    chr4107237758107237799+intron-Distal,intron-Proximal4.9537014.41880K562AQR
    chr4107237769107237881+intron-Distal,intron-Proximal3.788159.22906HepG2NCBP2
    ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




    eCLIP assay for AIMP1 is not avaliable on ENCORE database.



    Diffrential Expression

    • The Knockdown/knockout assay for AIMP1 is not available on ENCORE database.

    • 83 RBPs can affect the levels of AIMP1.  








    The Knockdown/knockout RNA-Seq assay for AIMP1 is not available on ENCORE database.





    83 RBPs can affect the gene expression of AIMP1 :

    Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
    AIMP1ENSG00000164022.121455.082401058.037900.459603.747102.65930HepG2ABCF1
    AIMP1ENSG00000164022.121257.52230993.975900.339204.375003.64380HepG2ACO1
    AIMP1ENSG00000164022.122628.925501917.740400.455003.526402.93510HepG2AKAP1
    AIMP1ENSG00000164022.121995.628801586.793700.330602.356701.62800HepG2ASCC1
    AIMP1ENSG00000164022.121459.650601914.27470-0.391206.387305.41310HepG2AUH
    AIMP1ENSG00000164022.12655.747201213.81210-0.888309.942908.37460HepG2BCLAF1
    AIMP1ENSG00000164022.12709.292601038.63410-0.550205.308204.51310HepG2BOP1
    AIMP1ENSG00000164022.12580.14710953.51920-0.716807.604906.51130HepG2CPSF6
    AIMP1ENSG00000164022.121131.62940862.934700.391003.334402.16270HepG2CSTF2
    AIMP1ENSG00000164022.121228.099001776.05030-0.532209.572808.51430HepG2CELF1
    AIMP1ENSG00000164022.121500.690701909.80610-0.347806.340105.28440HepG2DAZAP1
    AIMP1ENSG00000164022.121342.37510919.297500.546108.943807.60030HepG2DDX19B
    AIMP1ENSG00000164022.121342.384101041.926900.365403.296902.29570HepG2DDX24
    AIMP1ENSG00000164022.121115.992201837.10280-0.719108.337107.30080HepG2DDX3X
    AIMP1ENSG00000164022.12871.000201373.30310-0.656905.345004.31400HepG2DDX55
    AIMP1ENSG00000164022.121561.37290950.972400.715208.867207.79260HepG2DKC1
    AIMP1ENSG00000164022.12671.91840889.75250-0.405103.128302.44180HepG2DNAJC2
    AIMP1ENSG00000164022.12722.876201517.07860-1.0695016.0735014.72310HepG2EIF2S1
    AIMP1ENSG00000164022.121036.35070819.870100.337902.226801.40730HepG2EIF3D
    AIMP1ENSG00000164022.121702.775201005.616200.7597013.1749011.19400HepG2EIF3G
    AIMP1ENSG00000164022.121170.769001676.60960-0.518104.072103.30040HepG2EIF4A3
    AIMP1ENSG00000164022.122603.505401884.041000.466509.261907.90240HepG2EIF4G1
    AIMP1ENSG00000164022.121395.996701804.80870-0.370603.213302.33880HepG2EIF4G2
    AIMP1ENSG00000164022.121819.479302197.89900-0.272603.290002.64340HepG2ETF1
    AIMP1ENSG00000164022.121188.86560971.315600.291502.213801.46680HepG2EWSR1
    AIMP1ENSG00000164022.12756.314001068.41100-0.498403.180002.36150HepG2FIP1L1
    AIMP1ENSG00000164022.121277.908401691.44910-0.404503.082402.35210HepG2FXR1
    AIMP1ENSG00000164022.121161.157201569.16160-0.434403.203002.48990HepG2G3BP1
    AIMP1ENSG00000164022.121412.932601755.11060-0.312903.998202.90760HepG2HNRNPA2B1
    AIMP1ENSG00000164022.122163.066001588.697600.445104.432103.56450HepG2HNRNPK
    AIMP1ENSG00000164022.12953.669501164.26590-0.287902.146401.50330HepG2IGF2BP1
    AIMP1ENSG00000164022.12863.058101022.83440-0.245002.462001.76630HepG2ILF2
    AIMP1ENSG00000164022.121038.033201701.53820-0.713007.290406.30280HepG2MATR3
    AIMP1ENSG00000164022.121116.73600864.907500.368603.295302.41600HepG2MSI2
    AIMP1ENSG00000164022.12811.934401741.64270-1.1010016.8775015.39810HepG2NCBP2
    AIMP1ENSG00000164022.121079.125801551.15550-0.523504.622303.70030HepG2PABPC4
    AIMP1ENSG00000164022.121318.318801512.37180-0.198101.815601.30670HepG2PCBP1
    AIMP1ENSG00000164022.121117.935901538.91120-0.461106.997806.20780HepG2PTBP1
    AIMP1ENSG00000164022.121411.524801918.93820-0.4431010.068009.08440HepG2PUM1
    AIMP1ENSG00000164022.121343.064801666.64400-0.311403.146902.48730HepG2QKI
    AIMP1ENSG00000164022.121498.637001040.741300.525907.062205.77790HepG2RBM15
    AIMP1ENSG00000164022.121463.465601149.195800.348702.339601.36810HepG2RCC2
    AIMP1ENSG00000164022.122198.230901712.646800.360005.546104.82260HepG2NELFE
    AIMP1ENSG00000164022.12607.09150797.69580-0.393902.104301.56120HepG2RPS19
    AIMP1ENSG00000164022.121041.842401468.35290-0.495103.199702.34130HepG2RPS3A
    AIMP1ENSG00000164022.121061.96390891.725500.252002.456101.84340HepG2SAFB2
    AIMP1ENSG00000164022.121025.453501602.17590-0.6438013.1722012.03130HepG2SF3B4
    AIMP1ENSG00000164022.121297.877301733.24540-0.417308.011806.91190HepG2SLBP
    AIMP1ENSG00000164022.12800.447301168.29420-0.545503.628602.51820HepG2SMNDC1
    AIMP1ENSG00000164022.122695.692202074.832100.377607.425206.71330HepG2SRFBP1
    AIMP1ENSG00000164022.121992.713001672.336700.252802.962702.47770HepG2SRSF1
    AIMP1ENSG00000164022.121356.416601784.81620-0.396006.288605.39840HepG2SRSF5
    AIMP1ENSG00000164022.123121.501301360.545301.1980033.4588031.95180HepG2SRSF9
    AIMP1ENSG00000164022.122465.310601801.409700.452509.457808.62040HepG2SSB
    AIMP1ENSG00000164022.121631.295401104.112600.563003.036001.69370HepG2SSRP1
    AIMP1ENSG00000164022.12904.27880721.618600.325402.593701.68260HepG2SUPT6H
    AIMP1ENSG00000164022.121889.602501503.194600.330003.902903.25530HepG2TAF15
    AIMP1ENSG00000164022.12666.365301005.42190-0.593405.769704.77780HepG2TARDBP
    AIMP1ENSG00000164022.121817.023801381.195900.395604.498103.44030HepG2TIA1
    AIMP1ENSG00000164022.12814.22030972.89850-0.256901.802901.38120HepG2TIAL1
    AIMP1ENSG00000164022.12817.34610981.69560-0.264302.443301.90640HepG2TRIM56
    AIMP1ENSG00000164022.121163.98280905.193500.362704.599303.73660HepG2UPF1
    AIMP1ENSG00000164022.121462.86330873.928300.7431017.2657015.96040HepG2UTP3
    AIMP1ENSG00000164022.121709.104901345.836600.344602.791202.06400HepG2XRN2
    AIMP1ENSG00000164022.122455.659903178.11500-0.372103.838503.17000K562AQR
    AIMP1ENSG00000164022.121442.232101798.05530-0.318104.283303.07020K562BCLAF1
    AIMP1ENSG00000164022.12442.13380630.13910-0.511203.831402.54940K562CCAR1
    AIMP1ENSG00000164022.121807.810002266.48670-0.326204.345602.93820K562CPEB4
    AIMP1ENSG00000164022.12946.856901641.44620-0.7937012.4756010.80070K562CPSF6
    AIMP1ENSG00000164022.121070.811501322.84020-0.304902.523801.52950K562DDX3X
    AIMP1ENSG00000164022.123081.993202345.199300.394107.622006.61350K562DDX47
    AIMP1ENSG00000164022.122446.186002078.810900.234702.343701.54660K562DKC1
    AIMP1ENSG00000164022.121665.912102163.12860-0.376803.801202.98040K562EIF2S2
    AIMP1ENSG00000164022.121925.875901602.759000.264902.400801.65730K562EIF3G
    AIMP1ENSG00000164022.122923.133203628.08620-0.311704.490603.30630K562EIF4G2
    AIMP1ENSG00000164022.121190.449601670.04500-0.488404.855603.71820K562FMR1
    AIMP1ENSG00000164022.122487.971201843.019700.432802.903302.17660K562G3BP2
    AIMP1ENSG00000164022.121197.361001567.21330-0.388303.558102.66130K562GPKOW
    AIMP1ENSG00000164022.12864.072201212.27900-0.488504.971504.19390K562HNRNPC
    AIMP1ENSG00000164022.122809.952402026.371900.471504.609702.98380K562HNRNPK
    AIMP1ENSG00000164022.121203.216401529.75980-0.346402.377101.56790K562METAP2
    AIMP1ENSG00000164022.121147.967101501.57030-0.387402.783301.94960K562PCBP3
    AIMP1ENSG00000164022.12880.293501151.76740-0.387803.599902.67990K562POLR2G
    AIMP1ENSG00000164022.121787.422801362.884600.391103.361702.53190K562PRPF8
    AIMP1ENSG00000164022.123502.118004125.42610-0.236302.963901.97290K562PUM2
    AIMP1ENSG00000164022.121776.291901388.319000.355402.440301.67450K562RPS19
    AIMP1ENSG00000164022.121445.860901762.96800-0.286103.149002.27960K562SF3A3
    AIMP1ENSG00000164022.121469.639101917.65250-0.383903.142902.01080K562SRSF5
    AIMP1ENSG00000164022.121300.265301555.52860-0.258602.466301.36520K562SRSF7
    AIMP1ENSG00000164022.121027.066801319.68770-0.361702.241901.33220K562SUPV3L1
    AIMP1ENSG00000164022.121440.935101155.236500.318702.743401.92770K562SYNCRIP
    AIMP1ENSG00000164022.121602.341202055.28800-0.359202.951802.22090K562TARDBP
    AIMP1ENSG00000164022.121483.686701982.52310-0.418204.937103.79530K562TIA1
    AIMP1ENSG00000164022.121468.810001894.42690-0.367105.627504.64270K562UPF1
    AIMP1ENSG00000164022.122951.700103580.31890-0.278502.455501.65090K562ZC3H8
    Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".






    The Knockdown/knockout RNA-Seq assay for AIMP1 is not available on ENCORE database.





    3 RBPs can affect the splicing of AIMP1 :

    Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
    SEAIMP1ENSG00000164022.12chr4+107237683107237751107236852107237501107246141107246275-0.313000.000000.00017excludedHepG2DKC1
    SEAIMP1ENSG00000164022.12chr4+107237683107237751107236852107237501107246141107246275-0.254000.000000.00004excludedHepG2SSB
    SEAIMP1ENSG00000164022.12chr4+107237683107237751107236852107237501107246141107246275-0.374000.000000.00000excludedHepG2SRSF9

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".





    The Knockdown/knockout RNA-Seq assay for AIMP1 is not avaliable on ENCORE database.