CWF19L2

General Information

Gene Name       : CWF19L2
Gene ID             : ENSG00000152404.15
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: CWF19L2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CWF19L2ENSG00000152404.158.817.458.13ENCSR000AEF
HepG2CWF19L2ENSG00000152404.153.686.645.16ENCSR985KAT
K562CWF19L2ENSG00000152404.157.078.107.58ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CWF19L2-BGKcLV28-K562ENCSR582AOQCWF19L2K562ReleasedENCSR220DTX   CRISPRKD% = 74.2,79.9






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CWF19L2ENCAB287TFYA303-322A1Released, ENCODE4BGKcLV28-55, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CWF19L2K562CRISPRENCSR582AOQENCSR220DTXCWF19L2-BGKcLV28-55,CWF19L2-BGKcLV28-56NT-BGKcLV28-1,NT-BGKcLV28-274.2,79.9ReleasedENCODE4
CWF19L2HepG2CRISPRBGHcLV29-55,BGHcLV29-56BGHcLV29-1,BGHcLV29-2no clear band,NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CWF19L2Product_ID: A303-322A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CWF19L2-human		 Bethyl_A303-322A_1_CWF19L2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CWF19L2 specific antibody, A303-322A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CWF19L2-specific antibody, A303-322A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CWF19L2-K562-CRISPR-A303-322A.png<br>Caption: Western blot following CRISPR against CWF19L2 in K562 whole cell lysate using CWF19L2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CWF19L2. CWF19L2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for CWF19L2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • CWF19L2 RNA is bound by 10 proteins.  







eCLIP assay for CWF19L2 is not avaliable on ENCORE database.





10 proteins are bound to CWF19L2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr11107197533107197610-3'-UTR,intron-Distal3.201345.19836HepG2U2AF2
chr11107197680107197767-3'-UTR,CDS,intron-Distal4.461566.03821HepG2GRWD1
chr11107219631107219693-3'-UTR,CDS,intron-Distal,intron-Proximal3.715806.34662HepG2PRPF8
chr11107299653107299721-CDS,intron-Distal3.267146.02988K562UCHL5
chr11107328437107328508-CDS,intron-Distal4.2179510.73371K562ZNF622
chr11107326394107326447-CDS,intron-Distal5.391899.44272HepG2BCLAF1
chr11107288923107288954-CDS,intron-Distal4.542514.78571K562UCHL5
chr11107260888107260931-CDS,intron-Distal5.083557.49561HepG2BCLAF1
chr11107224323107224351-CDS,intron-Distal4.379144.07072K562UCHL5
chr11107309824107309873-CDS,intron-Distal3.616743.86355HepG2PPIG
chr11107328442107328503-CDS,intron-Distal3.835343.14999HepG2GRWD1
chr11107288954107289011-CDS,intron-Distal3.983074.01763HepG2GRWD1
chr11107326401107326464-CDS,intron-Distal5.338839.91399K562UCHL5
chr11107309832107309900-CDS,intron-Distal4.149357.14937K562ZNF622
chr11107326417107326475-CDS,intron-Distal3.089214.83973K562ZNF622
chr11107260713107260806-CDS,intron-Distal,intron-Proximal4.135683.12843K562PRPF8
chr11107300083107300106-CDS,intron-Distal4.400534.42734K562UCHL5
chr11107288976107289012-CDS,intron-Distal3.411824.85828HepG2BCLAF1
chr11107299590107299630-CDS,intron-Distal3.236224.74755K562ZNF622
chr11107313664107313679-CDS,intron-Distal,intron-Proximal3.970443.20743HepG2PPIG
chr11107312321107312350-CDS,intron-Distal,intron-Proximal3.233433.39477K562BUD13
chr11107260582107260682-intron-Distal4.377825.44709K562KHSRP
chr11107288111107288234-intron-Distal4.556516.09994K562KHSRP
chr11107201294107201383-intron-Distal4.733213.72755K562EIF4G2
chr11107198011107198067-intron-Distal4.484863.77802HepG2U2AF2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CWF19L2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CWF19L2 is not available on ENCORE database.

  • 54 RBPs can affect the levels of CWF19L2.  








The Knockdown/knockout RNA-Seq assay for CWF19L2 is not available on ENCORE database.





54 RBPs can affect the gene expression of CWF19L2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CWF19L2ENSG00000152404.11226.04400375.62450-0.732704.841603.97470HepG2AUH
CWF19L2ENSG00000152404.11135.18110213.74900-0.661002.265301.45260HepG2BCLAF1
CWF19L2ENSG00000152404.11150.43640298.71420-0.989606.879505.98350HepG2BOP1
CWF19L2ENSG00000152404.11166.43220257.42520-0.629204.040803.26590HepG2CKAP4
CWF19L2ENSG00000152404.11252.84330154.038900.714802.585301.84690HepG2CNOT7
CWF19L2ENSG00000152404.11131.24680196.89920-0.585202.741901.72880HepG2YBX3
CWF19L2ENSG00000152404.11183.78890289.90860-0.657504.281303.51320HepG2CELF1
CWF19L2ENSG00000152404.11217.23270311.73340-0.521103.492202.67670HepG2DAZAP1
CWF19L2ENSG00000152404.11151.44120241.81170-0.675102.304001.59240HepG2DDX55
CWF19L2ENSG00000152404.11135.37550227.56030-0.749302.334701.76340HepG2DDX6
CWF19L2ENSG00000152404.11123.61890193.34190-0.645303.072802.33590HepG2DNAJC21
CWF19L2ENSG00000152404.11117.85180191.63230-0.701403.952703.20100HepG2DNAJC2
CWF19L2ENSG00000152404.11173.42000294.60060-0.764504.619403.58250HepG2EIF4G2
CWF19L2ENSG00000152404.11223.56750310.90190-0.475701.906801.36910HepG2GPKOW
CWF19L2ENSG00000152404.11199.84870286.48690-0.519602.969602.00660HepG2HNRNPA2B1
CWF19L2ENSG00000152404.11110.45220217.11660-0.975005.138104.21570HepG2HNRNPC
CWF19L2ENSG00000152404.11202.72250276.99730-0.450402.186301.54330HepG2HNRNPF
CWF19L2ENSG00000152404.11213.98000283.96850-0.408302.213701.60120HepG2HNRNPL
CWF19L2ENSG00000152404.11133.17490220.31130-0.726204.931103.97440HepG2ILF2
CWF19L2ENSG00000152404.11168.32370238.39640-0.502102.415901.83950HepG2PCBP1
CWF19L2ENSG00000152404.11133.27870192.37050-0.529402.323901.64200HepG2PPIL4
CWF19L2ENSG00000152404.11132.65560295.07310-1.153409.240208.23740HepG2PUF60
CWF19L2ENSG00000152404.11229.21680313.00300-0.449502.285101.67350HepG2PUM2
CWF19L2ENSG00000152404.11203.94460293.68560-0.526102.400901.74150HepG2SF3B1
CWF19L2ENSG00000152404.11310.89390407.12170-0.389002.369601.89430HepG2SRFBP1
CWF19L2ENSG00000152404.11174.22630272.13080-0.643303.840103.04010HepG2SRSF7
CWF19L2ENSG00000152404.11440.23800353.485900.316502.007401.55280HepG2SSB
CWF19L2ENSG00000152404.11116.79390182.26150-0.642002.495101.59940HepG2SUPT6H
CWF19L2ENSG00000152404.11178.89510289.12390-0.692603.680202.85940HepG2TARDBP
CWF19L2ENSG00000152404.11137.18880209.57740-0.611302.854102.34770HepG2TIAL1
CWF19L2ENSG00000152404.11112.95730211.44790-0.904505.946605.17600HepG2TRIM56
CWF19L2ENSG00000152404.1179.77240142.23270-0.834302.978702.19380HepG2UCHL5
CWF19L2ENSG00000152404.11188.04350259.63530-0.465402.355501.70270HepG2UPF1
CWF19L2ENSG00000152404.11115.38630285.54950-1.3073014.2497013.24930HepG2ZNF622
CWF19L2ENSG00000152404.111042.48570797.247300.386801.909301.40500K562AQR
CWF19L2ENSG00000152404.111051.50180832.475400.336903.397502.37560K562ATP5C1
CWF19L2ENSG00000152404.11251.96770403.07600-0.677803.886302.63480K562EIF3D
CWF19L2ENSG00000152404.11380.35940490.97860-0.368302.456901.70580K562EIF3G
CWF19L2ENSG00000152404.11386.07490505.77930-0.389602.834001.66080K562EIF4B
CWF19L2ENSG00000152404.11424.98710563.32380-0.406502.475601.67490K562FIP1L1
CWF19L2ENSG00000152404.11987.78820662.939600.575202.558401.88290K562G3BP2
CWF19L2ENSG00000152404.11606.07990774.85460-0.354402.287201.59690K562GRSF1
CWF19L2ENSG00000152404.11206.79050390.65870-0.917706.446405.57300K562HNRNPC
CWF19L2ENSG00000152404.11532.70530755.08500-0.503303.842202.56890K562HNRNPL
CWF19L2ENSG00000152404.11844.38870587.489400.523205.820704.51200K562HNRNPU
CWF19L2ENSG00000152404.111170.46260841.944400.475204.659903.54130K562KIF1C
CWF19L2ENSG00000152404.11585.96760749.79160-0.355702.279801.51220K562MAGOH
CWF19L2ENSG00000152404.11500.23770639.49360-0.354302.172901.44990K562PPIG
CWF19L2ENSG00000152404.11498.81160728.44050-0.546303.642102.89140K562PTBP1
CWF19L2ENSG00000152404.11767.384801216.99430-0.665307.609306.25100K562PUF60
CWF19L2ENSG00000152404.111017.805601268.32310-0.317502.850301.87560K562PUM2
CWF19L2ENSG00000152404.111008.79750766.629100.395904.107103.40160K562RBM15
CWF19L2ENSG00000152404.11494.64670735.21970-0.571803.926102.59560K562NELFE
CWF19L2ENSG00000152404.11423.56730756.32830-0.836407.888306.08990K562RPS3
CWF19L2ENSG00000152404.11214.62970302.83930-0.496702.309101.39030K562SRFBP1
CWF19L2ENSG00000152404.11345.74450714.07100-1.0464010.938309.54070K562SRSF1
CWF19L2ENSG00000152404.11463.05950730.14580-0.657006.407604.68860K562SRSF4
CWF19L2ENSG00000152404.11988.58440650.763100.603102.873001.37600K562TAF15
CWF19L2ENSG00000152404.11381.06050722.78030-0.923507.439606.21650K562TARDBP
CWF19L2ENSG00000152404.11501.83620697.17860-0.474303.558902.61250K562TIA1
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CWF19L2 is not available on ENCORE database.





5 RBPs can affect the splicing of CWF19L2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECWF19L2ENSG00000152404.11chr11-107309815107309909107304359107304475107312228107312348-0.250000.000000.00000excludedK562AQR
SECWF19L2ENSG00000152404.11chr11-107304359107304475107299524107300177107312228107312348-0.152000.000010.00048excludedK562PUS1
SECWF19L2ENSG00000152404.11chr11-107286934107287024107263504107263621107288919107289013-0.155000.001080.02409excludedK562U2AF1
SECWF19L2ENSG00000152404.11chr11-107286934107287024107263504107263621107288919107289013-0.119000.000240.00902excludedK562AGGF1
SECWF19L2ENSG00000152404.11chr11-107299524107300177107288919107289013107304359107304475-0.188000.000040.00129excludedK562HNRNPC
A3SSCWF19L2ENSG00000152404.11chr11-1072635041072636431072635041072636211072869341072870240.071000.001090.00748longerK562AQR
MXECWF19L2ENSG00000152404.11chr11-107304359107304475107309815107309909107288919107289013107312228107312348-0.303000.000000.000002includedK562AQR
MXECWF19L2ENSG00000152404.11chr11-107304359107304475107309815107309909107288919107289013107312228107312348-0.220000.006290.082292includedK562HNRNPC

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CWF19L2 is not avaliable on ENCORE database.