CNOT4

General Information

Gene Name       : CNOT4
Gene ID             : ENSG00000080802.18
Essential Gene : No

Functions

Localization

Domains (AlphaFold: CNOT4)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CNOT4ENSG00000080802.1812.3811.4811.93ENCSR000AEF
HepG2CNOT4ENSG00000080802.185.678.006.83ENCSR985KAT
K562CNOT4ENSG00000080802.1812.0413.3312.68ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
RNA Bind-n-SeqRBNS: CNOT4ENCSR806UCECNOT4cell-free sampleReleasedENCSR566CVD  






  eCLIP  

No eCLIP experiment available



  Antibody Validation  

No Antibody Primary Validation image or Secondary image available



  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CNOT4Ribo-STAMP-293TshRNABGRSLV01-3,BGRSLV01-4BGRSLV01-1,BGRSLV01-2NotSatisfiedENCORE2
CNOT4Ribo-STAMP-293TshRNACNOT4-BGRSLV02-69,CNOT4-BGRSLV02-70NT-BGRSLV02-1,NT-BGRSLV02-2,SubmittedENCORE2
CNOT4Ribo-STAMP-293TshRNABGRSDLV03-5,BGRSDLV03-6BGRSDLV03-1,BGRSDLV03-2SubmittedENCORE2
CNOT4Ribo-STAMP-293TshRNACNOT4-BGRSDLV03-7dox,CNOT4-BGRSDLV03-8doxCNOT4-BGRSDLV03-5,CNOT4-BGRSDLV03-6,SubmittedENCORE2
CNOT4293TshRNACNOT4-BG293LV02-5,CNOT4-BG293LV02-6NT-BG293LV02-1,NT-BG293LV02-2,RevokedENCORE2
CNOT4293TshRNACNOT4-BG293LV06-95B,CNOT4-BG293LV06-96BNT-BG293LV06-1B,NT-BG293LV06-2B,SequencedENCORE2


  RNA Bind-n-Seqn  
  GeneName    gene_aliases    Status    Project    Note  
CNOT4CNOT4ReleasedENCODE3


  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela








RNA Targets

  • eCLIP assay for CNOT4 is not avaliable on ENCORE database.


  • The binding motifs of CNOT4

    • Trulli

Proteins bound to RNA
  • CNOT4 RNA is bound by 26 proteins.  







eCLIP assay for CNOT4 is not avaliable on ENCORE database.





26 proteins are bound to CNOT4 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr7135072875135072945-3'-UTR,intron-Distal3.012785.54525K562KHSRP
chr7135073547135073595-3'-UTR,CDS,intron-Distal4.372623.87582K562ZNF622
chr7135122901135123116-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.532997.89598HepG2GRWD1
chr7135073005135073089-3'-UTR,intron-Distal3.173746.82127K562KHSRP
chr7135072945135073005-3'-UTR,intron-Distal3.343187.14496K562KHSRP
chr7135072782135072889-3'-UTR,intron-Distal4.588795.37430HepG2KHSRP
chr7135073589135073663-CDS,intron-Distal3.286894.05586HepG2U2AF2
chr7135098329135098377-CDS,intron-Distal,intron-Proximal6.4539830.33710K562U2AF2
chr7135098311135098363-CDS,intron-Distal,intron-Proximal3.059404.03389K562ZNF622
chr7135078738135078773-CDS,intron-Distal3.398593.34231K562RBM15
chr7135080401135080491-CDS,intron-Distal4.262607.36824K562RBM15
chr7135095351135095453-CDS,intron-Distal,intron-Proximal5.538508.26501K562SF3B4
chr7135078835135078896-CDS,intron-Distal4.432435.80303K562IGF2BP1
chr7135098327135098383-CDS,intron-Distal,intron-Proximal5.4055126.84716HepG2U2AF2
chr7135107027135107125-CDS,intron-Distal,intron-Proximal4.326244.28193HepG2AQR
chr7135107014135107104-CDS,intron-Distal,intron-Proximal3.264316.54511K562UCHL5
chr7135082931135082987-CDS,intron-Distal,intron-Proximal3.659485.44908K562SF3B4
chr7135106804135106932-CDS,intron-Distal,intron-Proximal3.262474.98422K562PRPF8
chr7135080491135080538-CDS,intron-Distal4.650994.48994K562RBM15
chr7135098323135098380-CDS,intron-Distal,intron-Proximal3.801196.08151K562BUD13
chr7135098329135098368-CDS,intron-Distal,intron-Proximal5.4189721.57795K562U2AF1
chr7135122900135122977-CDS,intron-Distal,intron-Proximal3.287864.34695HepG2BCLAF1
chr7135078837135078895-CDS,intron-Distal4.504594.48994K562RBM15
chr7135078773135078829-CDS,intron-Distal3.867287.98536K562RBM15
chr7135107030135107112-CDS,intron-Distal,intron-Proximal3.225094.34695HepG2BCLAF1
chr7135106903135106939-CDS,intron-Distal,intron-Proximal4.4383711.41775K562LIN28B
chr7135099992135100011-CDS,intron-Distal5.019898.76639K562LIN28B
chr7135106873135107016-CDS,intron-Distal,intron-Proximal3.346595.40132HepG2BCLAF1
chr7135082924135082987-CDS,intron-Distal,intron-Proximal3.782253.00614K562U2AF2
chr7135095230135095343-CDS,intron-Distal,intron-Proximal5.376038.41647HepG2BCLAF1
chr7135194204135194286-intron-Distal3.365804.09699HepG2HNRNPK
chr7135176318135176377-intron-Distal5.2576710.55100HepG2SUGP2
chr7135092967135093023-intron-Distal5.7094114.07420K562KHSRP
chr7135177686135177753-intron-Distal3.974813.06015K562MATR3
chr7135117347135117422-intron-Distal3.609673.10466K562SAFB2
chr7135065945135066050-intron-Distal4.021133.22972K562TARDBP
chr7135087887135088003-intron-Distal4.7089414.82118K562KHSRP
chr7135194208135194320-intron-Distal5.0936013.68052HepG2PCBP2
chr7135194433135194520-intron-Distal,intron-Proximal4.2439610.39610HepG2PCBP2
chr7135173786135173844-intron-Distal4.856148.57760K562KHSRP
chr7135098368135098384-intron-Distal,intron-Proximal5.2933219.25102K562U2AF1
chr7135173903135173926-intron-Distal3.251804.08064K562KHSRP
chr7135106786135106864-intron-Distal,intron-Proximal3.021323.29251K562KHSRP
chr7135175623135175679-intron-Distal4.953778.41240HepG2SUGP2
chr7135100421135100537-intron-Distal4.416466.07567K562KHSRP
chr7135151679135151821-intron-Distal4.361235.44709K562KHSRP
chr7135176393135176446-intron-Distal4.654036.48506K562MATR3
chr7135106550135106685-intron-Distal4.5640510.28391K562KHSRP
chr7135078567135078623-intron-Distal,intron-Proximal3.834623.31110K562TIA1
chr7135088075135088143-intron-Distal3.159297.22523K562KHSRP
chr7135178275135178324-intron-Distal3.749704.35402HepG2SUGP2
chr7135093307135093408-intron-Distal5.163838.89278K562TARDBP
chr7135106685135106744-intron-Distal3.152833.90146K562KHSRP
chr7135142005135142077-intron-Distal3.966687.55880K562PTBP1
chr7135095135135095232-intron-Distal,intron-Proximal4.023883.71735K562TIA1
chr7135175633135175695-intron-Distal3.183293.04190HepG2MATR3
chr7135094350135094428-intron-Distal3.091765.85121K562KHSRP
chr7135093338135093402-intron-Distal3.895766.62875HepG2KHSRP
chr7135105454135105506-intron-Distal4.452553.05187HepG2QKI
chr7135173844135173872-intron-Distal4.962978.14619K562KHSRP
chr7135064137135064217-intron-Distal7.3210978.99279HepG2QKI
chr7135176327135176389-intron-Distal4.324174.51355HepG2PTBP1
chr7135175641135175681-intron-Distal3.931943.33944K562MATR3
chr7135064088135064137-intron-Distal5.0640414.62925HepG2QKI
chr7135177161135177220-intron-Distal4.502035.51760K562MATR3
chr7135177177135177219-intron-Distal4.328503.96879HepG2MATR3
chr7135179306135179399-intron-Distal3.265553.84025HepG2SUGP2
chr7135182647135182807-intron-Distal3.4335211.56460K562KHSRP
chr7135105361135105442-intron-Distal4.285228.42721HepG2QKI
chr7135174898135174959-intron-Distal3.210574.13620K562KHSRP
chr7135063505135063574-intron-Distal3.048884.10915HepG2QKI
chr7135092869135092919-intron-Distal4.503055.62258K562KHSRP
chr7135176812135176853-intron-Distal4.542924.31720HepG2MATR3
chr7135064132135064238-intron-Distal6.7642958.70260K562QKI
chr7135096458135096541-intron-Distal3.343813.07751K562KHSRP
chr7135177028135177094-intron-Distal5.4550611.33240HepG2SUGP2
chr7135173872135173903-intron-Distal4.665397.00634K562KHSRP
chr7135176082135176116-intron-Distal3.547504.35402HepG2SUGP2
chr7135092919135092967-intron-Distal5.048218.27925K562KHSRP
chr7135181601135181723-intron-Distal3.077313.14580K562ILF3
chr7135176404135176443-intron-Distal3.961844.22415K562PTBP1
chr7135176817135176873-intron-Distal3.557714.26619K562MATR3
chr7135175897135175980-intron-Distal5.5773713.08717HepG2SUGP2
chr7135082987135083034-intron-Distal,intron-Proximal4.200715.68247K562SF3B4
chr7135175911135175958-intron-Distal3.385063.98203K562MATR3
chr7135092688135092797-intron-Distal3.9363510.37908K562KHSRP
chr7135088013135088146-intron-Distal4.259314.26925HepG2KHSRP
chr7135176337135176386-intron-Distal4.343295.04209K562MATR3
chr7135106790135106881-intron-Distal,intron-Proximal4.466895.66560K562TIA1
chr7135133232135133277-intron-Distal4.258804.53347HepG2SUGP2
chr7135142010135142079-intron-Distal4.351334.07628HepG2PTBP1
chr7135171336135171511-intron-Distal3.288988.75128K562KHSRP
chr7135084664135084766-intron-Distal3.466995.32884K562KHSRP
chr7135175325135175432-intron-Distal3.3101413.19563HepG2SUGP2
chr7135176777135176860-intron-Distal3.442909.46590HepG2SUGP2
chr7135176769135176869-intron-Distal4.050874.04407K562HNRNPL
chr7135082992135083055-intron-Distal,intron-Proximal3.762763.58352HepG2CSTF2
chr7135176519135176586-intron-Distal4.592905.32928K562MATR3
chr7135175339135175388-intron-Distal3.885175.66122HepG2MATR3
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CNOT4 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CNOT4 is not available on ENCORE database.

  • 29 RBPs can affect the levels of CNOT4.  








The Knockdown/knockout RNA-Seq assay for CNOT4 is not available on ENCORE database.





29 RBPs can affect the gene expression of CNOT4 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CNOT4ENSG00000080802.14302.90320401.13340-0.405202.993502.29550HepG2HNRNPL
CNOT4ENSG00000080802.14281.02750384.14960-0.451003.139302.49650HepG2HNRNPU
CNOT4ENSG00000080802.14320.41890436.62940-0.446403.189602.37310HepG2NOL12
CNOT4ENSG00000080802.14696.81640515.499900.434704.250503.31520HepG2PRPF4
CNOT4ENSG00000080802.14688.99010535.506200.363503.157802.63460HepG2SRFBP1
CNOT4ENSG00000080802.14542.15420424.551500.352702.395301.94690HepG2SRSF1
CNOT4ENSG00000080802.141092.75490626.920100.801509.368508.20040HepG2U2AF1
CNOT4ENSG00000080802.14525.39220663.81220-0.337402.886002.17470HepG2UPF1
CNOT4ENSG00000080802.14951.99460719.511000.403803.168001.89250K562AARS
CNOT4ENSG00000080802.142481.77490999.806101.3116032.5833030.77310K562AQR
CNOT4ENSG00000080802.14907.78890567.805400.676906.984405.13190K562CCAR1
CNOT4ENSG00000080802.142110.149801702.383600.309704.163003.38560K562DDX47
CNOT4ENSG00000080802.14809.99160622.007400.380902.756701.93750K562EIF4A3
CNOT4ENSG00000080802.14774.93160646.861900.260501.845401.33130K562FXR1
CNOT4ENSG00000080802.14954.861601221.21030-0.354902.262801.57630K562GRSF1
CNOT4ENSG00000080802.141847.460001106.771500.739102.598101.42760K562HNRNPA2B1
CNOT4ENSG00000080802.141094.13740787.544200.474304.697903.94030K562HNRNPC
CNOT4ENSG00000080802.141461.640501806.32340-0.305502.474801.65080K562METAP2
CNOT4ENSG00000080802.141072.571701776.43040-0.727902.011801.31610K562PPIG
CNOT4ENSG00000080802.14891.35680543.868800.712607.017305.82420K562PRPF8
CNOT4ENSG00000080802.142062.141001554.910200.407204.137203.14620K562PUF60
CNOT4ENSG00000080802.141944.230101366.174100.509005.244104.52720K562PUS1
CNOT4ENSG00000080802.141559.126001331.196900.227902.035301.51240K562RBM15
CNOT4ENSG00000080802.141645.08350884.653700.894903.853602.36440K562SF1
CNOT4ENSG00000080802.142147.539101016.396901.079104.861503.08920K562SNRNP200
CNOT4ENSG00000080802.141374.813101021.100600.429002.947602.09830K562SYNCRIP
CNOT4ENSG00000080802.14919.78670595.953600.626005.152003.56600K562U2AF1
CNOT4ENSG00000080802.14999.33760615.543900.699009.869208.35030K562U2AF2
CNOT4ENSG00000080802.141411.409701205.394800.227502.395501.60360K562WDR43
CNOT4ENSG00000080802.142105.601401195.826900.816103.070901.38070K562YTHDC2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CNOT4 is not available on ENCORE database.





5 RBPs can affect the splicing of CNOT4 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECNOT4ENSG00000080802.14chr7-1350974861350976331350952641350953981350982361350983620.053000.005120.06334includedK562PUS1
SECNOT4ENSG00000080802.14chr7-135048605135048818135047750135047938135078669135079167-0.258000.001350.03866excludedHepG2SF3A3
SECNOT4ENSG00000080802.14chr7-1350974861350976331350952641350953981350982361350983620.078000.000840.02404includedK562AGGF1
A3SSCNOT4ENSG00000080802.14chr7-1350952641350954681350952641350953981350982361350984060.187000.000000.00001longerK562AQR
MXECNOT4ENSG00000080802.14chr7-135099079135099181135099924135100011135098236135098362135106904135107102-0.210000.000130.001442includedHepG2U2AF2

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CNOT4 is not avaliable on ENCORE database.