ABT1

General Information

Gene Name       : ABT1
Gene ID             : ENSG00000146109.4
Essential Gene : Yes

Functions

Localization(RBPImage: ABT1)

Domains (AlphaFold: ABT1)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ABT1ENSG00000146109.45.3010.497.89ENCSR000AEF
HepG2ABT1ENSG00000146109.431.0916.9023.99ENCSR985KAT
K562ABT1ENSG00000146109.429.2819.9124.59ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq ABT1-BGKLV38-K562ENCSR233UVMABT1K562ReleasedENCSR260BQC   shRNAKD% = 57.1,58.4
knockdown followed by RNA-seq ABT1-BGHLV37-HepG2ENCSR756VLWABT1HepG2ReleasedENCSR225PRV   shRNAKD% = 78.7,79.7






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ABT1ENCAB891MJIA304-044A1Released, ENCODE3BGHLV37-3, Released, ENCODE3BGKLV38-5, Released, ENCODE3


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ABT1K562shRNABGKLV29-5,BGKLV29-6BGKLV29-1,BGKLV29-2NotSatisfiedENCODE3
ABT1K562shRNABGKLV32-3,BGKLV32-4BGKLV32-1,BGKLV32-2NotSatisfiedENCODE3
ABT1K562shRNABGKLV34-3,BGKLV34-4BGKLV34-1,BGKLV34-2NotSatisfiedENCODE3
ABT1K562shRNABGKLV38-3,BGKLV38-4BGKLV38-1,BGKLV38-24.9,4.7NotSatisfiedENCODE3
ABT1K562shRNAENCSR233UVMENCSR260BQCABT1-BGKLV38-5C,ABT1-BGKLV38-6CNT-BGKLV38-1C,NT-BGKLV38-2C57.1,58.4ReleasedENCODE4
ABT1HepG2shRNABGHLV30-5,BGHLV30-6BGHLV30-1,BGHLV30-2NotSatisfiedENCODE3
ABT1HepG2shRNABGHLV31-3,BGHLV31-4BGHLV31-1,BGHLV31-20.0,0.0NotSatisfiedENCODE3
ABT1HepG2shRNAENCSR756VLWENCSR225PRVABT1-BGHLV37-3C,ABT1-BGHLV37-4Cnon-target-BGHLV37-1C,non-target-BGHLV37-2C78.7,79.7ReleasedENCODE4
ABT1HepG2shRNABGHLV37-5,BGHLV37-6BGHLV37-1,BGHLV37-222.2,27.5NotSatisfiedENCODE3
ABT1HepG2shRNABGHLV37-7,BGHLV37-8BGHLV37-1,BGHLV37-20.9,23.2NotSatisfiedENCODE3


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  
    ABT1: Click to RBP Image Database
  • Nuclei
  • Nucleolus
  • Nuclear release mitosis












    • RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
    ABT1Product_ID: A304-044A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: ABT1-human    		 ABT1-hepg2.png<br>Caption: Western blot following shRNA against ABT1 in HepG2 whole cell lysate using ABT1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ABT1.ABT1 protein appears as the green band, Tubulin serves as a control and appears in red.
    		 K562_Bethyl_A304-044A_1_ABT1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using ABT1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ABT1 antibody (lanes under 'ABT1').
    		 ABT1-K562.png<br>Caption: Western blot following shRNA against ABT1 in k562 whole cell lysate using ABT1 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ABT1.ABT1 protein appears as the green band, Tubulin serves as a control and appears in red.








    RNA Targets

    • eCLIP assay for ABT1 is not avaliable on ENCORE database.



    Proteins bound to RNA
    • ABT1 RNA is bound by 13 proteins.  







    eCLIP assay for ABT1 is not avaliable on ENCORE database.





    13 proteins are bound to ABT1 RNA:

    Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
    chr62659937726599433+3'-UTR4.153463.29239HepG2CSTF2
    chr62659927726599352+3'-UTR3.549176.36921K562DDX6
    chr62659938026599431+3'-UTR5.2224610.69195K562CSTF2T
    chr62659887426598960+3'-UTR3.578538.40205K562PABPC4
    chr62659885326598966+3'-UTR,CDS4.1930711.06468HepG2LARP4
    chr62659720726597336+5'-UTR,CDS3.845917.61543K562GRWD1
    chr62659720526597263+5'-UTR,CDS3.220376.24463HepG2GRWD1
    chr62659849526598555+CDS3.630939.09662K562YBX3
    chr62659721126597271+CDS3.272134.89070K562UCHL5
    chr62659744826597503+CDS,intron-Proximal3.176913.20886K562PRPF8
    chr62659827226598316+CDS3.491923.69775K562YBX3
    chr62659849926598530+CDS4.316064.15208K562RBM15
    chr62659868926598727+CDS3.433924.22460K562RBM15
    chr62659810726598173+CDS,intron-Proximal4.809374.62947HepG2AQR
    chr62659726326597345+CDS4.961097.83063HepG2GRWD1
    chr62659831626598338+CDS4.091766.19204K562YBX3
    chr62659727126597338+CDS4.966516.44794K562UCHL5
    chr62659853026598550+CDS4.747314.48994K562RBM15
    chr62659863726598689+CDS5.7130111.79327K562RBM15
    chr62659770126597763+intron-Distal3.893395.28701K562PRPF8
    chr62659764226597754+intron-Distal3.171173.80845K562RBFOX2
    chr62659749226597607+intron-Distal,intron-Proximal3.852734.77128HepG2RBFOX2
    chr62659763826597706+intron-Distal4.632063.48173K562GTF2F1
    chr62659745126597534+intron-Proximal4.326129.29543K562AQR
    ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




    eCLIP assay for ABT1 is not avaliable on ENCORE database.



    Diffrential Expression

    • The Knockdown/knockout assay for ABT1 is not available on ENCORE database.

    • 80 RBPs can affect the levels of ABT1.  








    The Knockdown/knockout RNA-Seq assay for ABT1 is not available on ENCORE database.





    80 RBPs can affect the gene expression of ABT1 :

    Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
    ABT1ENSG00000146109.31695.861901335.973800.344001.816001.37530HepG2AKAP1
    ABT1ENSG00000146109.3798.961001105.31760-0.468302.592101.83090HepG2ASCC1
    ABT1ENSG00000146109.31063.221301266.03740-0.251902.438501.78640HepG2AUH
    ABT1ENSG00000146109.3924.69490441.563901.066308.769207.43440HepG2CNOT7
    ABT1ENSG00000146109.3348.86950481.73230-0.465501.887801.30830HepG2CPSF6
    ABT1ENSG00000146109.31078.70320912.103400.241902.541001.82560HepG2DAZAP1
    ABT1ENSG00000146109.3489.753701099.62630-1.166908.948707.90270HepG2DDX6
    ABT1ENSG00000146109.3645.11200495.701800.380003.049102.31540HepG2DNAJC21
    ABT1ENSG00000146109.3769.085801056.65310-0.458302.320401.77390HepG2EIF2S1
    ABT1ENSG00000146109.3438.66520566.17610-0.368102.326601.33400HepG2EIF3G
    ABT1ENSG00000146109.31242.182401453.66210-0.226802.513301.93600HepG2ETF1
    ABT1ENSG00000146109.3571.14570819.56580-0.521004.721603.71050HepG2FUBP3
    ABT1ENSG00000146109.3686.214801093.00070-0.671603.829903.05170HepG2G3BP1
    ABT1ENSG00000146109.31157.63800830.833000.478508.300107.18990HepG2HNRNPL
    ABT1ENSG00000146109.3361.49970521.07520-0.527502.240301.66780HepG2HNRNPLL
    ABT1ENSG00000146109.3701.21610552.954700.342602.506101.92870HepG2HNRNPU
    ABT1ENSG00000146109.3816.008101185.52750-0.538902.933002.28610HepG2MATR3
    ABT1ENSG00000146109.3939.366501213.36730-0.369301.856401.33100HepG2NCBP2
    ABT1ENSG00000146109.31150.52110904.444000.347103.456402.60520HepG2NOL12
    ABT1ENSG00000146109.3797.117501080.18760-0.438402.296901.63460HepG2PABPC4
    ABT1ENSG00000146109.3694.71790493.195700.494204.962804.00430HepG2PPIL4
    ABT1ENSG00000146109.3340.28920620.09510-0.8657011.112109.71090HepG2PRPF4
    ABT1ENSG00000146109.3776.685701169.94580-0.5910010.024109.10960HepG2PTBP1
    ABT1ENSG00000146109.31042.00200863.392200.271201.880001.37480HepG2PUF60
    ABT1ENSG00000146109.3798.212401267.02710-0.6666010.208609.11560HepG2QKI
    ABT1ENSG00000146109.3829.777101149.92860-0.470704.088003.28220HepG2RBM25
    ABT1ENSG00000146109.3860.92150556.710600.628904.643903.26490HepG2RCC2
    ABT1ENSG00000146109.3943.559701302.01660-0.464606.373005.60900HepG2NELFE
    ABT1ENSG00000146109.3842.678301212.68910-0.525207.876206.91420HepG2RECQL
    ABT1ENSG00000146109.3597.88420443.646400.430402.049101.36060HepG2RPS3A
    ABT1ENSG00000146109.3673.61510492.445300.451903.192902.51130HepG2SAFB2
    ABT1ENSG00000146109.3881.888701218.01990-0.465906.359305.52430HepG2SF3B4
    ABT1ENSG00000146109.31001.15820827.909800.274002.924402.20430HepG2SLBP
    ABT1ENSG00000146109.3214.28640573.84490-1.421108.987007.58530HepG2SNRNP70
    ABT1ENSG00000146109.31103.676201271.36730-0.204101.707501.31070HepG2SRSF1
    ABT1ENSG00000146109.31090.44940852.504000.355004.187203.44590HepG2SRSF5
    ABT1ENSG00000146109.3715.117401034.34070-0.532508.195007.33520HepG2SRSF9
    ABT1ENSG00000146109.3541.96020386.008900.489502.639201.56870HepG2STIP1
    ABT1ENSG00000146109.31279.61260969.506300.400306.089705.23260HepG2SUCLG1
    ABT1ENSG00000146109.3457.77930728.69860-0.670705.103104.22910HepG2SUPV3L1
    ABT1ENSG00000146109.3463.267501142.80510-1.3027033.3529031.68490HepG2TAF15
    ABT1ENSG00000146109.3729.762901050.02850-0.524905.993004.77070HepG2TIA1
    ABT1ENSG00000146109.31136.15720843.538400.429504.220803.11410HepG2TUFM
    ABT1ENSG00000146109.31022.01210813.406500.329303.411702.75880HepG2UTP3
    ABT1ENSG00000146109.3425.42150611.91950-0.524405.074204.21220HepG2ZC3H8
    ABT1ENSG00000146109.3987.86490701.542700.493706.055605.26760K562AGGF1
    ABT1ENSG00000146109.3939.61780729.971800.364102.514201.89060K562AKAP8L
    ABT1ENSG00000146109.32483.720801456.007800.770406.439305.60550K562AQR
    ABT1ENSG00000146109.3353.13750507.28810-0.522603.443302.23970K562CCAR1
    ABT1ENSG00000146109.3417.57180597.44120-0.516802.539301.78700K562CPSF6
    ABT1ENSG00000146109.31823.458402182.60950-0.259402.234001.35770K562CELF1
    ABT1ENSG00000146109.31671.710201214.843100.460505.635804.75590K562DDX47
    ABT1ENSG00000146109.3845.88070577.484100.550604.617303.42270K562EFTUD2
    ABT1ENSG00000146109.3669.89360481.011200.477804.433103.43620K562EIF3G
    ABT1ENSG00000146109.3818.11930625.215800.387902.738101.92120K562EIF4A3
    ABT1ENSG00000146109.3993.02920764.425400.377402.878202.01190K562FIP1L1
    ABT1ENSG00000146109.3327.88360515.13600-0.651803.166502.18350K562FTO
    ABT1ENSG00000146109.31255.39880650.191600.9491020.9789019.42520K562FXR1
    ABT1ENSG00000146109.3750.34660959.47620-0.354703.266502.02430K562GLRX3
    ABT1ENSG00000146109.32016.756601691.808700.253402.031601.37810K562GRSF1
    ABT1ENSG00000146109.3456.91080656.54940-0.523004.134403.41960K562HNRNPC
    ABT1ENSG00000146109.3960.07250628.813800.610405.162803.84690K562HNRNPM
    ABT1ENSG00000146109.31333.114301036.310200.363202.924002.10740K562HSPD1
    ABT1ENSG00000146109.3363.99140529.99360-0.542103.559201.86130K562ILF3
    ABT1ENSG00000146109.3796.59540583.758800.448403.781002.78960K562MAGOH
    ABT1ENSG00000146109.3809.539901008.76590-0.317403.156102.29340K562MTPAP
    ABT1ENSG00000146109.3806.67680569.117100.503203.538102.03460K562NONO
    ABT1ENSG00000146109.3827.117201120.81970-0.438406.925305.81920K562NSUN2
    ABT1ENSG00000146109.31324.50200867.864900.609806.877005.48210K562PPIG
    ABT1ENSG00000146109.31174.51370561.959601.0634015.6689013.86310K562PRPF8
    ABT1ENSG00000146109.3900.81130567.181000.667308.367307.25300K562PTBP1
    ABT1ENSG00000146109.31204.03350724.040400.733607.587006.73370K562PUS1
    ABT1ENSG00000146109.31019.87780705.378300.531806.171105.31110K562RBM15
    ABT1ENSG00000146109.3875.69190543.692500.687507.588606.10910K562RBM22
    ABT1ENSG00000146109.3795.35700572.188200.475003.625102.35210K562NELFE
    ABT1ENSG00000146109.3947.50370572.387300.727006.368405.11720K562RPS19
    ABT1ENSG00000146109.3478.23970329.724900.536402.808401.79880K562RPS3
    ABT1ENSG00000146109.3465.06650362.133200.360802.250901.48160K562SF3B4
    ABT1ENSG00000146109.3414.79110558.73890-0.429802.697901.45170K562SLBP
    ABT1ENSG00000146109.3941.689301133.09920-0.267002.769701.83310K562SRPK2
    ABT1ENSG00000146109.31107.86810556.060600.994406.807605.70800K562SRSF1
    ABT1ENSG00000146109.3746.57950942.38480-0.336002.939801.73240K562SRSF7
    ABT1ENSG00000146109.3173.58170341.75840-0.977404.826002.67960K562TAF15
    ABT1ENSG00000146109.3734.87930562.696000.385102.471201.80040K562TARDBP
    ABT1ENSG00000146109.3686.05550542.803200.337802.264501.53120K562TIA1
    ABT1ENSG00000146109.3695.20720532.825700.383702.454801.43910K562TRA2A
    ABT1ENSG00000146109.3977.137001188.42970-0.282402.993802.24620K562UPF1
    ABT1ENSG00000146109.3729.535301016.90050-0.479104.795603.62760K562WDR3
    ABT1ENSG00000146109.3873.189301057.46720-0.276202.802801.95130K562WDR43
    Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".






    The Knockdown/knockout RNA-Seq assay for ABT1 is not available on ENCORE database.






    No RBPs affect the alternative splcing events of ABT1 on ENCORE database.





    The Knockdown/knockout RNA-Seq assay for ABT1 is not avaliable on ENCORE database.