ATP5F1A

General Information

Gene Name       : ATP5F1A
Gene ID             : ENSG00000152234.15
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: ATP5F1A)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ATP5F1AENSG00000152234.15433.47429.69431.58ENCSR000AEF
HepG2ATP5F1AENSG00000152234.15442.11453.60447.86ENCSR985KAT
K562ATP5F1AENSG00000152234.15285.88293.04289.46ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq ATP5A1-BGKcLV26-K562ENCSR328DHDATP5A1K562ReleasedENCSR900GQL   CRISPRKD% = 61.7,72.2
knockdown followed by RNA-seq ATP5A1-BGHcLV29-HepG2ENCSR340ZLQATP5A1HepG2ReleasedENCSR462MFN   CRISPRKD% = 82.5,82.0






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ATP5A1ENCAB205IOVA304-939A1Released, ENCODE4BGHcLV29-9, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ATP5A1K562CRISPRENCSR328DHDENCSR900GQLATP5A1-BGKcLV26-37,ATP5A1-BGKcLV26-38NT-BGKcLV26-1,NT-BGKcLV26-261.7,72.2ReleasedENCODE4
ATP5A1HepG2CRISPRBGHcLV27-21,BGHcLV27-22BGHcLV27-1,BGHcLV27-2NotSatisfiedENCODE4
ATP5A1HepG2CRISPRENCSR340ZLQENCSR462MFNATP5A1-BGHcLV29-9,ATP5A1-BGHcLV29-10NT-BGHcLV29-1,NT-BGHcLV29-282.5,82.0ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ATP5F1AProduct_ID: A304-939A
Lot_ID: 1
Source: Bethyl Labs
Target Name: ATP5F1A-human		 ATP5A1-K562-CRISPR-A304-939A.png<br>Caption: Western blot following CRISPR against ATP5F1A in K562 whole cell lysate using ATP5F1A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ATP5F1A. ATP5F1A protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for ATP5F1A is not avaliable on ENCORE database.



Proteins bound to RNA

  • NO known proteins bind on ATP5F1A RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for ATP5F1A is not avaliable on ENCORE database.






NO known proteins are bound to ATP5F1A RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for ATP5F1A is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for ATP5F1A is not available on ENCORE database.

  • No RBPs affect the gene expression of ATP5F1A on ENCORE database.


The Knockdown/knockout RNA-Seq assay for ATP5F1A is not available on ENCORE database.






No RBPs affect the gene expression of ATP5F1A on ENCORE database.






The Knockdown/knockout RNA-Seq assay for ATP5F1A is not available on ENCORE database.






No RBPs affect the alternative splcing events of ATP5F1A on ENCORE database.





The Knockdown/knockout RNA-Seq assay for ATP5F1A is not avaliable on ENCORE database.