EIF3A

General Information

Gene Name : EIF3A
Gene ID : ENSG00000107581.12
Essential Gene : Yes

Functions

Ribosome & basic translation
Translation regulation
Viral RNA regulation

Localization(RBPImage: EIF3A)

Nuclei
Nucleoplasm
Cytoplasm
Cytosolic

Domains (AlphaFold: EIF3A)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

View validation images for all EIF3A antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	EIF3A	ENSG00000107581.12	138.32	132.37	135.34	ENCSR000AEF
HepG2	EIF3A	ENSG00000107581.12	93.44	108.89	101.16	ENCSR985KAT
K562	EIF3A	ENSG00000107581.12	103.38	109.96	106.67	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 4138	ENCSR311SHM	EIF3A	K562	Released	ENCAB543LFV	ENCSR724YPP
knockdown followed by RNA-seq	EIF3A-BGKLV24-K562	ENCSR258VGD	EIF3A	K562	Released		ENCSR667PLJ	shRNA	KD% = 67.4,87.8

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
EIF3A	4138	K562	ENCSR311SHM	ENCSR724YPP	ENCAB543LFV	3411S	1	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	K562 Primary Validation	K562 Secondary Validation
EIF3A	ENCAB265WCJ	A302-002A	1		BGKLV24-21, Released, ENCODE3
EIF3A		3411S	3	Submitted, ENCORE2	BGKLV24-21, Submitted, ENCORE2
EIF3A		A302-003A	2		BGKLV24-21, Submitted, ENCORE2
EIF3A	ENCAB543LFV	3411S	1	Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
EIF3A	K562	shRNA	ENCSR258VGD	ENCSR667PLJ	EIF3A_BGKLV24_21_17613,EIF3A_BGKLV24_22_17614	non-target_BGKLV24_1_17594,non-target_BGKLV24_2_17595	67.4,87.8	Released	ENCODE3
EIF3A	HepG2	shRNA			BGHLV22-13,BGHLV22-14	BGHLV22-1,BGHLV22-2	0.0,0.0	NotSatisfied	ENCODE3
EIF3A	HepG2	CRISPR			BGHcLV12-31,BGHcLV12-32	BGHcLV12-1,BGHcLV12-2	no band on the position,NO MORE AB	NotSatisfied	ENCODE4
EIF3A	HepG2	CRISPR			BGHcLV12-33,BGHcLV12-34	BGHcLV12-1,BGHcLV12-2	no band on the position,	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

EIF3A: Click to RBP Image Database

Nuclei

Nucleoplasm

Cytoplasm

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
EIF3A	Product_ID: 3411S Lot_ID: 3 Source: Cell Signaling Technology Target Name: EIF3A-human
EIF3A	Product_ID: A302-002A Lot_ID: 1 Source: Bethyl Labs Target Name: EIF3A-human
EIF3A	Product_ID: A302-003A Lot_ID: 2 Source: Bethyl Labs Target Name: EIF3A-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for EIF3A is not avaliable on ENCORE database.

Proteins bound to RNA

EIF3A RNA is bound by 42 proteins.

CanvasJS.com

eCLIP assay for EIF3A is not avaliable on ENCORE database.

42 proteins are bound to EIF3A RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr10	120802196	120802284	-	CDS,intron-Distal	5.60030	400.00000	K562	UCHL5
chr10	120819614	120819626	-	intron-Distal	5.25153	400.00000	HepG2	DKC1
chr10	120819598	120819605	-	intron-Distal	5.01225	400.00000	HepG2	DKC1
chr10	120819588	120819592	-	intron-Distal	4.80834	400.00000	HepG2	DKC1
chr10	120819583	120819588	-	intron-Distal	4.10800	400.00000	HepG2	DKC1
chr10	120819626	120819639	-	intron-Distal	5.08614	400.00000	HepG2	DKC1
chr10	120819525	120819540	-	intron-Distal	3.16212	400.00000	HepG2	DKC1
chr10	120819521	120819525	-	intron-Distal	4.06549	400.00000	HepG2	DKC1
chr10	120819606	120819614	-	intron-Distal	5.27013	400.00000	HepG2	DKC1
chr10	120819579	120819582	-	intron-Distal	3.57160	400.00000	HepG2	DKC1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 357 entries

eCLIP assay for EIF3A is not avaliable on ENCORE database.

Diffrential Expression

2,876 differentially expressed genes after Knockdown/Knockout EIF3A protein.

CanvasJS.com

118 RBPs can affect the levels of EIF3A.

CanvasJS.com

2,876 differentially expressed genes after Knockdown/Knockout EIF3A protein :

Only display 100 entries as example. Please click "View All" button to see all 2,876 entries.

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
IGF1	ENSG00000017427.11	735.97420	43.56280	4.07840	62.31290	58.57480	K562	EIF3A
SLC4A1	ENSG00000004939.9	921.38730	140.80300	2.71000	40.77540	37.58140	K562	EIF3A
ACSM3	ENSG00000005187.7	251.05150	1063.87150	-2.08330	27.73130	25.03460	K562	EIF3A
TMSB10	ENSG00000034510.4	590.48830	2125.33610	-1.84770	27.13900	24.48020	K562	EIF3A
CREBBP	ENSG00000005339.8	2899.07710	915.39060	1.66300	23.85870	21.27450	K562	EIF3A
RNF10	ENSG00000022840.11	612.64520	1712.78310	-1.48320	17.43510	15.03450	K562	EIF3A
ITGA2B	ENSG00000005961.13	61.35230	293.63080	-2.25880	16.81410	14.44250	K562	EIF3A
SLC25A5	ENSG00000005022.5	1670.78420	4171.75150	-1.32010	16.49790	14.14460	K562	EIF3A
VIM	ENSG00000026025.9	6995.91800	3162.79990	1.14520	13.20280	11.03880	K562	EIF3A
TKTL1	ENSG00000007350.12	174.53850	12.29910	3.82680	12.52540	10.41570	K562	EIF3A
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

118 RBPs can affect the gene expression of EIF3A :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
EIF3A	ENSG00000107581.8	2752.72210	11502.93160	-2.06310	99.87510	97.53220	HepG2	BOP1
EIF3A	ENSG00000107581.8	2271.21200	5732.04090	-1.33560	69.57670	65.81930	HepG2	YBX3
EIF3A	ENSG00000107581.8	4817.69410	8229.93220	-0.77250	41.04400	39.22900	HepG2	PUS1
EIF3A	ENSG00000107581.8	6301.72950	10779.07370	-0.77440	40.06370	38.65230	HepG2	SSB
EIF3A	ENSG00000107581.8	1491.90250	4923.32620	-1.72250	25.69150	23.06740	K562	EIF3A
EIF3A	ENSG00000107581.8	3431.12540	6100.54460	-0.83030	24.17610	22.97230	HepG2	TIAL1
EIF3A	ENSG00000107581.8	4647.92180	9033.34920	-0.95870	24.56930	22.86670	K562	HNRNPC
EIF3A	ENSG00000107581.8	6895.87340	10991.98020	-0.67260	23.27550	22.06830	HepG2	RBM22
EIF3A	ENSG00000107581.8	3397.35560	6374.98720	-0.90800	21.76390	20.10350	HepG2	DDX21
EIF3A	ENSG00000107581.8	3533.63590	6320.45240	-0.83890	18.80810	17.16730	HepG2	HNRNPC
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 141 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for EIF3A is not available on ENCORE database.

6 RBPs can affect the splicing of EIF3A :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	is_included	Cell Line	Affected by RBP
SE	EIF3A	ENSG00000107581.8	chr10	-	120824910	120825082			120820735	120820840	120828957	120829166	-0.13200	excluded	K562	AQR
A3SS	EIF3A	ENSG00000107581.8	chr10	-	120810032	120810223			120810032	120810186	120810710	120810833	0.12600	longer	HepG2	SF3A3
A3SS	EIF3A	ENSG00000107581.8	chr10	-	120810032	120810253			120810032	120810186	120810710	120810896	0.15400	longer	K562	AQR
A3SS	EIF3A	ENSG00000107581.8	chr10	-	120810710	120810941			120810710	120810833	120816251	120816331	0.05700	longer	K562	AQR
A3SS	EIF3A	ENSG00000107581.8	chr10	-	120832952	120833119			120832952	120833089	120833258	120833449	0.10200	longer	K562	AQR
A3SS	EIF3A	ENSG00000107581.8	chr10	-	120820735	120820901			120820735	120820840	120824910	120825126	0.10400	longer	K562	AQR
A3SS	EIF3A	ENSG00000107581.8	chr10	-	120809312	120809547			120809312	120809497	120810032	120810186	0.09200	longer	K562	AQR
A3SS	EIF3A	ENSG00000107581.8	chr10	-	120796630	120796855			120796630	120796821	120797749	120797951	0.05500	longer	K562	AQR
MXE	EIF3A	ENSG00000107581.8	chr10	-	120820257	120820356	120820735	120820867	120819113	120819230	120824910	120825082	0.05100	1included	K562	AQR
MXE	EIF3A	ENSG00000107581.8	chr10	-	120820735	120820840	120824910	120825082	120819113	120819230	120828957	120829166	-0.11700	2included	K562	AQR

Showing 1 to 10 of 17 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

EIF3A

General Information

Functions

Localization(RBPImage: EIF3A)

Domains (AlphaFold: EIF3A)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for EIF3A is not avaliable on ENCORE database.

42 proteins are bound to EIF3A RNA:

eCLIP assay for EIF3A is not avaliable on ENCORE database.

Diffrential Expression

2,876 differentially expressed genes after Knockdown/Knockout EIF3A protein :

118 RBPs can affect the gene expression of EIF3A :

The Knockdown/knockout RNA-Seq assay for EIF3A is not available on ENCORE database.

6 RBPs can affect the splicing of EIF3A :

The Knockdown/knockout RNA-Seq assay for EIF3A is not avaliable on ENCORE database.