DUS3L

General Information

Gene Name       : DUS3L
Gene ID             : ENSG00000141994.15
Essential Gene : No

Functions

Localization

Domains (AlphaFold: DUS3L)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878DUS3LENSG00000141994.156.2119.0512.63ENCSR000AEF
HepG2DUS3LENSG00000141994.1532.0710.8621.46ENCSR985KAT
K562DUS3LENSG00000141994.1558.5256.2757.40ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq DUS3L-BGKcLV17-K562ENCSR949QWRDUS3LK562ReleasedENCSR516TLX   CRISPRKD% = 68.1,70.4
knockdown followed by RNA-seq DUS3L-BGHcLV18-HepG2ENCSR020EBNDUS3LHepG2ReleasedENCSR670KAS   CRISPRKD% = 77.9,75.5






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
DUS3LENCAB967CUWPA5-59757UC2739268AReleased, ENCODE4BGHcLV18-13, Released, ENCODE4BGKcLV17-13, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
DUS3LK562CRISPRENCSR949QWRENCSR516TLXDUS3L-BGKcLV17-13,DUS3L-BGKcLV17-14NT-BGKcLV17-1,NT-BGKcLV17-268.1,70.4ReleasedENCODE4
DUS3LHepG2CRISPRENCSR020EBNENCSR670KASDUS3L-BGHcLV18-13,DUS3L-BGHcLV18-14NT-BGHcLV18-1,NT-BGHcLV18-277.9,75.5ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DUS3LProduct_ID: PA5-59757
Lot_ID: UC2739268A
Source: Thermo Fisher
Target Name: DUS3L-human		 DUS3L-HEPG2-CRISPR-PA5-59757.png<br>Caption: Western blot following CRISPR against DUS3L in HepG2 whole cell lysate using DUS3L specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DUS3L. DUS3L protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 ThermoFisher_PA5-59757_UC2739268A_DUS3L.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DUS3L specific antibody, PA5-59757. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DUS3L-specific antibody, PA5-59757. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DUS3L-K562-CRISPR-PA5-59757.png<br>Caption: Western blot following CRISPR against DUS3L in K562 whole cell lysate using DUS3L specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DUS3L. DUS3L protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for DUS3L is not avaliable on ENCORE database.



Proteins bound to RNA
  • DUS3L RNA is bound by 15 proteins.  







eCLIP assay for DUS3L is not avaliable on ENCORE database.





15 proteins are bound to DUS3L RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr1957883655788414-3'-UTR,CDS,intron-Distal,intron-Proximal3.812705.51703K562BUD13
chr1957864955786539-3'-UTR,CDS,intron-Distal4.339933.83824K562BUD13
chr1957854515785473-3'-UTR,CDS,intron-Distal,intron-Proximal3.299283.69775K562YBX3
chr1957864715786537-3'-UTR,CDS,intron-Distal,intron-Proximal5.147447.60796HepG2PPIG
chr1957891555789225-3'-UTR,CDS,intron-Distal,intron-Proximal4.904328.30573K562AQR
chr1957879805788042-3'-UTR,CDS,intron-Distal,intron-Proximal4.376855.32062K562EFTUD2
chr1957883985788425-3'-UTR,CDS,intron-Distal,intron-Proximal4.734824.86944K562AQR
chr1957897155789760-3'-UTR,CDS,intron-Distal,intron-Proximal3.296205.07297K562BUD13
chr1957883315788376-3'-UTR,CDS,intron-Distal,intron-Proximal4.704035.35117HepG2PRPF8
chr1957881655788221-3'-UTR,CDS,intron-Distal,intron-Proximal3.424414.77826K562AQR
chr1957879945788047-3'-UTR,CDS,intron-Distal,intron-Proximal3.667504.83899HepG2PRPF8
chr1957854825785523-3'-UTR,CDS,intron-Distal,intron-Proximal3.186453.47879HepG2PPIG
chr1957901555790214-5'-UTR,CDS,intron-Distal,intron-Proximal3.391104.59873K562UCHL5
chr1957910525791170-5'-UTR,CDS,intron-Distal,intron-Proximal3.648367.76798K562DDX3X
chr1957902265790283-5'-UTR,CDS,intron-Distal,intron-Proximal4.217503.81597K562RBM15
chr1957900165790057-5'-UTR,CDS,intron-Distal,intron-Proximal4.066315.97994K562AQR
chr1957900115790061-5'-UTR,CDS,intron-Distal,intron-Proximal3.635684.93376K562PRPF8
chr1957900165790067-5'-UTR,CDS,intron-Distal,intron-Proximal5.022744.68690HepG2PRPF8
chr1957870295787103-CDS,intron-Distal,intron-Proximal4.1612811.94608HepG2PRPF8
chr1957852805785356-CDS,intron-Distal,intron-Proximal3.086453.13705K562BUD13
chr1957853035785374-CDS,intron-Distal,intron-Proximal5.199006.69228HepG2PRPF8
chr1957873415787371-CDS,intron-Distal4.347413.79199HepG2PPIG
chr1957873425787394-CDS,intron-Distal,intron-Proximal4.6823912.46803K562AQR
chr1957903205790382-CDS,intron-Distal,intron-Proximal4.496853.36385K562UCHL5
chr1957868735787007-CDS,intron-Distal,intron-Proximal5.045416.51490HepG2PCBP2
chr1957858635785905-intron-Distal,intron-Proximal4.481388.03807K562RBM15
chr1957875355787554-intron-Distal,intron-Proximal4.846024.86944K562AQR
chr1957875545787584-intron-Distal,intron-Proximal5.3414114.59371K562AQR
chr1957870255787065-intron-Distal,intron-Proximal3.603315.83621K562AQR
chr1957903485790358-intron-Distal,intron-Proximal4.653074.65563K562AQR
chr1957864355786474-intron-Distal,intron-Proximal4.888895.65342K562AQR
chr1957910105791055-intron-Distal,intron-Proximal4.492789.80019K562AQR
chr1957875845787599-intron-Distal,intron-Proximal4.921109.85896K562AQR
chr1957872035787255-intron-Distal,intron-Proximal4.903185.88499HepG2DDX59
chr1957871875787256-intron-Distal,intron-Proximal4.180683.33442K562HNRNPK
chr1957872025787262-intron-Distal,intron-Proximal4.139675.12778HepG2PCBP2
chr1957867475786758-intron-Distal,intron-Proximal4.874885.69951K562AQR
chr1957872865787305-intron-Distal,intron-Proximal3.450884.63311K562AQR
chr1957867155786747-intron-Distal,intron-Proximal5.388897.81430K562AQR
chr1957909865791006-intron-Distal,intron-Proximal4.831154.09264K562AQR
chr1957858245785904-intron-Distal,intron-Proximal4.861685.40008K562SF3B4
chr1957885695788660-intron-Distal3.403633.57928K562GTF2F1
chr1957871945787265-intron-Distal,intron-Proximal4.167244.84707HepG2HNRNPK
chr1957886315788703-intron-Distal4.282623.56466K562LSM11
chr1957883215788362-intron-Distal,intron-Proximal4.153076.39176K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for DUS3L is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for DUS3L is not available on ENCORE database.

  • 90 RBPs can affect the levels of DUS3L.  








The Knockdown/knockout RNA-Seq assay for DUS3L is not available on ENCORE database.





90 RBPs can affect the gene expression of DUS3L :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
DUS3LENSG00000141994.1159.63210111.08310-0.897502.882001.79130HepG2AATF
DUS3LENSG00000141994.11371.90960209.682700.826602.108401.63720HepG2AKAP1
DUS3LENSG00000141994.11239.44350152.706000.648802.577701.67570HepG2BCCIP
DUS3LENSG00000141994.1177.66870129.96100-0.742702.349501.63490HepG2CALR
DUS3LENSG00000141994.11133.1645079.209800.749402.924401.87780HepG2YBX3
DUS3LENSG00000141994.1158.41420113.76540-0.961703.032401.59010HepG2CSTF2T
DUS3LENSG00000141994.11188.44930129.307300.543302.596001.97160HepG2CELF1
DUS3LENSG00000141994.11213.84590138.993900.621402.948302.18680HepG2DAZAP1
DUS3LENSG00000141994.1173.05410122.48970-0.745602.398001.76140HepG2DDX21
DUS3LENSG00000141994.11288.31870172.633000.739901.840901.32650HepG2DDX6
DUS3LENSG00000141994.11173.65340277.07600-0.674104.251003.44660HepG2DKC1
DUS3LENSG00000141994.11131.2228077.773100.754602.943802.22230HepG2DNAJC21
DUS3LENSG00000141994.11129.6802077.093600.750201.883301.32720HepG2DNAJC2
DUS3LENSG00000141994.11135.87150238.06120-0.809103.600502.87910HepG2GPKOW
DUS3LENSG00000141994.11301.27610173.941500.792402.574001.89900HepG2HNRNPK
DUS3LENSG00000141994.11267.59480126.599701.079706.436905.45020HepG2HNRNPL
DUS3LENSG00000141994.11108.63590227.18890-1.064405.997205.10730HepG2HNRNPLL
DUS3LENSG00000141994.11149.6240086.761800.786102.994702.36640HepG2HNRNPU
DUS3LENSG00000141994.11386.00580186.092501.052506.371905.44650HepG2MATR3
DUS3LENSG00000141994.11136.31760217.60450-0.674703.316702.43430HepG2MSI2
DUS3LENSG00000141994.11131.29080231.77000-0.819903.192602.14560HepG2MTPAP
DUS3LENSG00000141994.11321.35420137.828401.221205.847004.74210HepG2NOL12
DUS3LENSG00000141994.11264.82640137.850100.941803.814302.51430HepG2NONO
DUS3LENSG00000141994.11125.71440201.46860-0.680402.259201.58170HepG2NSUN2
DUS3LENSG00000141994.11130.6003076.610800.769402.186701.41210HepG2PABPC1
DUS3LENSG00000141994.11156.7610077.384201.018405.400504.40060HepG2PPIL4
DUS3LENSG00000141994.11118.16870178.39150-0.594202.197601.69920HepG2PTBP1
DUS3LENSG00000141994.11261.41650131.581300.990307.184606.28070HepG2PUF60
DUS3LENSG00000141994.11381.16690139.693301.4481022.4299021.07590HepG2PUM1
DUS3LENSG00000141994.11121.06600294.98600-1.2849013.9847012.78350HepG2PUS1
DUS3LENSG00000141994.11262.87100134.157100.970304.941703.59810HepG2RBM27
DUS3LENSG00000141994.11197.76550108.942600.860103.224302.07900HepG2RCC2
DUS3LENSG00000141994.11131.67320190.75850-0.534801.926101.34390HepG2RPLP0
DUS3LENSG00000141994.11171.4430077.263801.149807.149606.18330HepG2SAFB2
DUS3LENSG00000141994.11176.4701079.304901.153805.693603.81890HepG2SART3
DUS3LENSG00000141994.11444.34270284.023800.645603.678702.89810HepG2SF3A3
DUS3LENSG00000141994.11268.84000185.708400.533602.826402.22970HepG2SF3B4
DUS3LENSG00000141994.11235.23770126.194700.898406.831105.80820HepG2SLBP
DUS3LENSG00000141994.11149.9793090.100300.735103.142302.46370HepG2SMN1
DUS3LENSG00000141994.11177.2982094.399600.909203.009102.00590HepG2SMNDC1
DUS3LENSG00000141994.1199.12820250.19640-1.335707.319206.04340HepG2SNRNP70
DUS3LENSG00000141994.11219.82300372.09680-0.759306.785106.09730HepG2SRFBP1
DUS3LENSG00000141994.11123.83050193.84760-0.646602.826702.34960HepG2SRSF1
DUS3LENSG00000141994.11283.86770129.934101.1273010.920809.78890HepG2SRSF5
DUS3LENSG00000141994.11184.74810323.05420-0.806206.971106.22780HepG2SSB
DUS3LENSG00000141994.11261.91840163.675200.678203.261101.87360HepG2SSRP1
DUS3LENSG00000141994.11287.70830147.756500.961308.809007.80460HepG2SUCLG1
DUS3LENSG00000141994.1159.71080174.26070-1.545209.989809.03060HepG2TAF15
DUS3LENSG00000141994.1193.92640221.52000-1.237805.213004.26260HepG2TARDBP
DUS3LENSG00000141994.11187.5450085.059501.140607.759306.89210HepG2TRIM56
DUS3LENSG00000141994.11203.52140128.572200.662503.236702.28390HepG2TUFM
DUS3LENSG00000141994.11276.41240136.106701.022005.587804.37160HepG2UBE2L3
DUS3LENSG00000141994.11163.4747088.297500.888504.143703.21420HepG2UCHL5
DUS3LENSG00000141994.11170.4004075.439901.175405.926204.32590HepG2XPO5
DUS3LENSG00000141994.11118.01800175.58640-0.573202.498301.85480HepG2ZC3H8
DUS3LENSG00000141994.111380.17530965.780100.515005.201603.42280K562APOBEC3C
DUS3LENSG00000141994.11479.32690694.92370-0.535804.924802.88950K562CEBPZ
DUS3LENSG00000141994.111025.33730794.662400.367603.791702.48800K562CPEB4
DUS3LENSG00000141994.11253.88690393.37520-0.631704.561903.55570K562CPSF6
DUS3LENSG00000141994.111262.28270822.231600.6183010.941809.74380K562DDX47
DUS3LENSG00000141994.11676.35920325.019301.0572011.988609.93860K562EFTUD2
DUS3LENSG00000141994.111007.77620817.978500.300902.731302.01640K562EIF2S2
DUS3LENSG00000141994.11623.00390347.840800.840706.089104.46740K562EIF3D
DUS3LENSG00000141994.11328.12700141.121301.2172010.844909.26190K562EIF3G
DUS3LENSG00000141994.11390.05420215.672600.854703.489101.31570K562EIF3H
DUS3LENSG00000141994.111392.199201094.530500.347003.520902.46310K562EIF4G2
DUS3LENSG00000141994.11250.68520404.99520-0.692004.464202.96970K562FKBP4
DUS3LENSG00000141994.11235.54850400.24630-0.764905.553604.33240K562FMR1
DUS3LENSG00000141994.11224.82880339.24330-0.593503.191002.20430K562FTO
DUS3LENSG00000141994.11805.30470420.284900.9381011.7041010.54880K562FXR1
DUS3LENSG00000141994.117.7710057.58470-2.889508.193106.84520K562G3BP2
DUS3LENSG00000141994.11274.41530375.57810-0.452802.352901.61930K562GPKOW
DUS3LENSG00000141994.11330.32770466.24640-0.497203.175302.54110K562HNRNPC
DUS3LENSG00000141994.11302.65210180.981700.741704.537202.92890K562IGF2BP3
DUS3LENSG00000141994.11183.11140320.69290-0.808504.114602.95590K562KRR1
DUS3LENSG00000141994.11474.58480186.883301.3444013.5078011.52530K562MAGOH
DUS3LENSG00000141994.11351.71220186.473700.915307.566805.94430K562MATR3
DUS3LENSG00000141994.11926.736201166.64610-0.332102.575801.48810K562MSI2
DUS3LENSG00000141994.11645.67300834.66470-0.370403.525202.61760K562MTPAP
DUS3LENSG00000141994.11275.10700403.58310-0.552902.564601.38070K562NCBP2
DUS3LENSG00000141994.11576.41560320.632500.846104.796402.95660K562NONO
DUS3LENSG00000141994.11774.35280927.33300-0.260102.504701.79060K562NSUN2
DUS3LENSG00000141994.11577.90890848.90000-0.554802.500601.34070K562PABPC1
DUS3LENSG00000141994.11392.36800166.243201.238808.827707.05080K562PCBP2
DUS3LENSG00000141994.11439.59700574.01890-0.384902.564401.79610K562POLR2G
DUS3LENSG00000141994.11847.12120316.285201.4212016.9008015.01770K562PRPF8
DUS3LENSG00000141994.11352.74230181.583900.957908.952807.80160K562PTBP1
DUS3LENSG00000141994.111644.609001194.203600.461604.739903.67510K562PUF60
DUS3LENSG00000141994.11478.26830622.78410-0.380902.291301.78720K562PUS1
DUS3LENSG00000141994.11376.12850174.007501.112007.245105.80580K562RBM22
DUS3LENSG00000141994.11341.26320169.708101.007703.199101.66960K562RBM34
DUS3LENSG00000141994.11560.27760322.058600.798702.075701.37260K562RPS19
DUS3LENSG00000141994.11186.2972059.859401.6379012.8956010.96270K562SF3B4
DUS3LENSG00000141994.11315.52920178.035300.825505.804304.78830K562SRSF1
DUS3LENSG00000141994.11325.28450205.292700.663903.072601.94940K562SRSF4
DUS3LENSG00000141994.11324.21810168.196700.946705.649104.04490K562SRSF5
DUS3LENSG00000141994.11478.78970636.95400-0.411802.529601.33700K562STAU1
DUS3LENSG00000141994.11798.78160575.782900.472204.382303.34340K562SYNCRIP
DUS3LENSG00000141994.11513.71950180.134701.5118020.5180018.24030K562TARDBP
DUS3LENSG00000141994.11455.87880173.773101.3913018.3001016.04300K562TIA1
DUS3LENSG00000141994.11398.46160170.638101.2234012.324109.47900K562TRA2A
DUS3LENSG00000141994.11770.86170983.27520-0.351103.794802.96660K562UPF1
DUS3LENSG00000141994.11622.34480841.35050-0.435003.682002.67270K562WDR3
DUS3LENSG00000141994.11515.30070330.078700.642503.420502.03020K562XRN2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for DUS3L is not available on ENCORE database.





6 RBPs can affect the splicing of DUS3L :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEDUS3LENSG00000141994.11chr19-578675957868565786477578655357870715787182-0.344000.000290.02353excludedHepG2RBM22
SEDUS3LENSG00000141994.11chr19-578675957868565786477578655357870715787182-0.252000.000170.04886excludedK562PUM1
A3SSDUS3LENSG00000141994.11chr19-5785613578584157856135785802578647757865680.164000.000000.00004longerK562AQR
A3SSDUS3LENSG00000141994.11chr19-5787306578741257873065787372578759957877160.152000.000000.00000longerK562AQR
A3SSDUS3LENSG00000141994.11chr19-5786477578662357864775786553578675957868560.417000.000000.00000longerK562AQR
A3SSDUS3LENSG00000141994.11chr19-5786759578693057867595786856578707157871820.507000.000000.00000longerK562AQR
A3SSDUS3LENSG00000141994.11chr19-5786759578693057867595786856578707157871820.300000.001150.05435longerK562PUS1
A5SSDUS3LENSG00000141994.11chr19-578675957871825787071578718257864775786553-0.621000.000390.03882shorterHepG2RBM22
RIDUS3LENSG00000141994.11chr19-5786759578718257867595786856578707157871820.331000.000020.00025includedK562PUS1
RIDUS3LENSG00000141994.11chr19-578675957871825786759578685657870715787182-0.759000.000190.00674excludedK562SRSF1
RIDUS3LENSG00000141994.11chr19-5786759578718257867595786856578707157871820.563000.006000.07837includedHepG2FUBP3
RIDUS3LENSG00000141994.11chr19-5786759578718257867595786856578707157871820.159000.007080.05867includedHepG2RBM22
RIDUS3LENSG00000141994.11chr19-5786759578718257867595786930578707157871820.344000.000010.00447includedK562PUM1
RIDUS3LENSG00000141994.11chr19-5786759578718257867595786856578707157871820.530000.000000.00000includedK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for DUS3L is not avaliable on ENCORE database.