GSK3B

General Information

Gene Name : GSK3B
Gene ID : ENSG00000082701.15
Essential Gene : No

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: GSK3B)

Not Available

Antibody Validation

Thermo Fisher: MA3-038

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	GSK3B	ENSG00000082701.15	27.34	28.25	27.80	ENCSR000AEF
HepG2	GSK3B	ENSG00000082701.15	18.69	21.22	19.95	ENCSR985KAT
K562	GSK3B	ENSG00000082701.15	15.03	15.57	15.30	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	GSK3B-BGKcLV15-K562	ENCSR025XBH	GSK3B	K562	Released		ENCSR404OHQ	CRISPR	KD% = 72.0,65.0
knockdown followed by RNA-seq	GSK3B-BGHcLV16-HepG2	ENCSR426JCD	GSK3B	HepG2	Released		ENCSR964HKT	CRISPR	KD% = 54.9,61.4

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
GSK3A	ENCAB957NOP	MA3-038	RF227934				BGHcLV16-31, Released, ENCODE4	BGKcLV15-37, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
GSK3B	K562	CRISPR	ENCSR025XBH	ENCSR404OHQ	GSK3B-BGKcLV15-37,GSK3B-BGKcLV15-38	NT-BGKcLV15-1,NT-BGKcLV15-2	72.0,65.0	Released	ENCODE4
GSK3B	HepG2	CRISPR	ENCSR426JCD	ENCSR964HKT	GSK3B-BGHcLV16-31,GSK3B-BGHcLV16-32	NT-BGHcLV16-1,NT-BGHcLV16-2	54.9,61.4	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
GSK3A	Product_ID: MA3-038 Lot_ID: RF227934 Source: Thermo Fisher Target Name: GSK3A-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for GSK3B is not avaliable on ENCORE database.

Proteins bound to RNA

GSK3B RNA is bound by 25 proteins.

CanvasJS.com

eCLIP assay for GSK3B is not avaliable on ENCORE database.

25 proteins are bound to GSK3B RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr3	119813004	119813137	-	5'-UTR,intron-Distal	3.91708	31.96003	HepG2	DDX3X
chr3	119812669	119812805	-	5'-UTR,intron-Distal	4.56273	24.13590	HepG2	PCBP2
chr3	119640958	119641083	-	intron-Distal	5.41957	19.79046	HepG2	PCBP2
chr3	119662467	119662648	-	intron-Distal	5.07303	18.60142	K562	PTBP1
chr3	119812912	119812999	-	5'-UTR,intron-Distal	3.03268	15.22068	HepG2	DDX3X
chr3	119662739	119662858	-	intron-Distal	4.35172	15.16976	K562	PTBP1
chr3	119662552	119662666	-	intron-Distal	3.71374	13.79150	HepG2	PTBP1
chr3	119662763	119662857	-	intron-Distal	4.49576	13.63692	HepG2	PTBP1
chr3	119542826	119542877	-	3'-UTR,intron-Distal	4.91713	12.31980	K562	PUM2
chr3	119813211	119813260	-	5'-UTR,intron-Distal	3.75734	11.34202	HepG2	DDX3X
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 80 entries

eCLIP assay for GSK3B is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for GSK3B is not available on ENCORE database.
73 RBPs can affect the levels of GSK3B.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for GSK3B is not available on ENCORE database.

73 RBPs can affect the gene expression of GSK3B :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
GSK3B	ENSG00000082701.10	3636.33920	1970.64030	0.88370	40.12760	38.80010	HepG2	SRFBP1
GSK3B	ENSG00000082701.10	2555.65450	1420.93410	0.84680	29.63210	28.44860	HepG2	SRSF1
GSK3B	ENSG00000082701.10	3239.94270	1744.73030	0.89290	29.04520	27.72080	HepG2	RBM22
GSK3B	ENSG00000082701.10	3014.12870	1818.18920	0.72910	22.69680	21.07560	HepG2	AUH
GSK3B	ENSG00000082701.10	2192.33510	1307.60680	0.74540	20.66610	19.43720	HepG2	PTBP1
GSK3B	ENSG00000082701.10	825.52980	1447.89530	-0.81060	20.58760	19.23000	HepG2	TRIM56
GSK3B	ENSG00000082701.10	3017.43930	1514.31980	0.99460	19.51760	17.79750	HepG2	U2AF1
GSK3B	ENSG00000082701.10	2058.28020	1331.36420	0.62840	17.73660	16.29470	HepG2	ZC3H8
GSK3B	ENSG00000082701.10	2561.87850	1676.32670	0.61180	16.75160	15.46100	HepG2	UTP3
GSK3B	ENSG00000082701.10	806.43980	1315.23250	-0.70570	16.06760	14.70750	HepG2	SAFB2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 81 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for GSK3B is not available on ENCORE database.

11 RBPs can affect the splicing of GSK3B :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	GSK3B	ENSG00000082701.10	chr3	-	119585436	119585475			119582265	119582452	119595259	119595355	0.56500	0.00000	0.00000	included	HepG2	U2AF1
SE	GSK3B	ENSG00000082701.10	chr3	-	119585436	119585475			119582265	119582452	119595259	119595355	0.32100	0.00000	0.00000	included	HepG2	U2AF2
SE	GSK3B	ENSG00000082701.10	chr3	-	119562101	119562200			119545532	119545702	119582265	119582452	-0.24200	0.00000	0.00000	excluded	K562	AQR
SE	GSK3B	ENSG00000082701.10	chr3	-	119585436	119585475			119582265	119582452	119595259	119595355	0.35700	0.00000	0.00000	included	K562	U2AF1
SE	GSK3B	ENSG00000082701.10	chr3	-	119585436	119585475			119582265	119582452	119595259	119595355	0.23500	0.00000	0.00017	included	K562	U2AF2
MXE	GSK3B	ENSG00000082701.10	chr3	-	119595259	119595355	119624601	119624699	119582265	119582452	119631550	119631657	0.28300	0.00000	0.00000	1included	HepG2	U2AF1
MXE	GSK3B	ENSG00000082701.10	chr3	-	119595259	119595355	119624601	119624699	119585436	119585475	119631550	119631657	-0.34900	0.00000	0.00000	2included	HepG2	U2AF1
MXE	GSK3B	ENSG00000082701.10	chr3	-	119585436	119585475	119595259	119595355	119582265	119582452	119624601	119624699	-0.40100	0.00000	0.00000	2included	HepG2	U2AF1
MXE	GSK3B	ENSG00000082701.10	chr3	-	119595259	119595355	119624601	119624699	119582265	119582452	119631550	119631657	0.15900	0.00000	0.00019	1included	HepG2	FTO
SE	GSK3B	ENSG00000082701.10	chr3	-	119585436	119585475			119582265	119582452	119595259	119595355	0.15400	0.00001	0.00064	included	HepG2	HNRNPC

Showing 1 to 10 of 26 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

GSK3B

General Information

Functions

Localization

Domains (AlphaFold: GSK3B)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for GSK3B is not avaliable on ENCORE database.

25 proteins are bound to GSK3B RNA:

eCLIP assay for GSK3B is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for GSK3B is not available on ENCORE database.

73 RBPs can affect the gene expression of GSK3B :

The Knockdown/knockout RNA-Seq assay for GSK3B is not available on ENCORE database.

11 RBPs can affect the splicing of GSK3B :

The Knockdown/knockout RNA-Seq assay for GSK3B is not avaliable on ENCORE database.