non-target      CRISPR in K562      Control: NT-BGKcLV42-1,NT-BGKcLV42-2

General Information

List KD experiments controlled by BGKcLV42-1 and BGKcLV42-2


NT-BGKcLV42-1,NT-BGKcLV42-2:
  • ADD1-BGKcLV42-K562
  • ASS1-BGKcLV42-K562
  • BCAT2-BGKcLV42-K562
  • BRD4-BGKcLV42-K562
  • CAB39-BGKcLV42-K562
  • CDK11B-BGKcLV42-K562
  • CNOT3-BGKcLV42-K562
  • CRKL-BGKcLV42-K562
  • DDX41-BGKcLV42-K562
  • DHX29-BGKcLV42-K562
  • DROSHA-BGKcLV42-K562
  • EIF2AK2-BGKcLV42-K562
  • EIF1-BGKcLV42-K562
  • EIF2AK4-BGKcLV42-K562
  • EIF4H-BGKcLV42-K562
  • GFPT1-BGKcLV42-K562
  • HEXIM1-BGKcLV42-K562
  • HSPA9-BGKcLV42-K562
  • KARS-BGKcLV42-K562
  • LARP1-BGKcLV42-K562
  • MAPK1-BGKcLV42-K562
  • NAA10-BGKcLV42-K562
  • NELFCD-BGKcLV42-K562
  • NOP2-BGKcLV42-K562
  • PABPC1-BGKcLV42-K562
  • PARK7-BGKcLV42-K562
  • PKM-BGKcLV42-K562
  • PKN2-BGKcLV42-K562
  • PRKRA-BGKcLV42-K562
  • PSMD4-BGKcLV42-K562
  • RBBP5-BGKcLV42-K562
  • RBM10-BGKcLV42-K562
  • RBMX-BGKcLV42-K562
  • SEC24D-BGKcLV42-K562
  • SESN2-BGKcLV42-K562
  • SSU72-BGKcLV42-K562
  • SYMPK-BGKcLV42-K562
  • TFAM-BGKcLV42-K562
  • UBA2-BGKcLV42-K562
  • USP15-BGKcLV42-K562
  • USP39-BGKcLV42-K562
  • VASP-BGKcLV42-K562
  • WDR77-BGKcLV42-K562
  • YTHDF2-BGKcLV42-K562




    • Experiment Information (Status: Submitting)
    BGKcLV42-1BGKcLV42-2
    idx00
    TRCN#_or_BGC#
    shRNA_or_gRNA_sequence
    PAM
    Namenonnon
    Sample_IDBGKcLV42-1BGKcLV42-2
    transduction_Date4/23/244/23/24
    daysD6D6
    RBP_namenon-targetnon-target
    qPCR_result
    Ave_qPCR
    RT-qPCR_primer-F
    RT-qPCR_primer-R
    protein_conc02792
    WB_result
    Ave_WB
    WB_DONE_date
    MW
    IP
    antibody_Cat#
    Antibody DCC ID
    submitted_to_DCC_date
    Rep1_TPM00
    Rep2_TPM00
    ActionReadyReady
    Library_start_date
    repeat_library
    Note
    ID57535754




    Western Blot
    Western Blot info was not avaliable




    Experiment Status
    BGKcLV42-1BGKcLV42-2
    Sample_IDBGKcLV42-1BGKcLV42-2
    Sample Name
    Sample NameNT-BGKcLV42-1NT-BGKcLV42-2
    RBPnon-targetnon-target
    Cell_LineK562K562
    Exp UID
    StatusSubmittingSubmitting
    Status_date2025-02-132025-02-13
    ProjectENCORE2ENCORE2
    Note
    ID1304413045




    Library-Prep Information
    BGKcLV42-1BGKcLV42-2
    Sample #12
    Sample NameNT-BGKcLV42-1NT-BGKcLV42-2
    Sample_Name_Alias
    Index Well PositionA01B01
    Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
    LibPrep_date2024-06-212024-06-21
    Lib_IDLib-240621Lib-240621
    Tecan_Location
    Tecan
    Tecan_date
    Size_bp294303
    Peak_Molarity59.5091.90
    libSampleQC_DNA_WellDNA_Library_set73/set1/A1.pngDNA_Library_set73/set1/B1.png
    RIN10.010.0
    libSample_RNA_WellRNA_Library_set73/set1/A1.pngRNA_Library_set73/set1/B1.png
    SampleQC_methodTapeStation_2022TapeStation_2022
    SampleQC_date2024-07-152024-07-15
    Sample_IDBGKcLV42-1BGKcLV42-2
    RBPNTNT
    Batch_IDBGKcLV42BGKcLV42
    WB_result0.0000.000
    Library DescriptionTruSeq mRNATruSeq mRNA
    Repeat_Library_Suffix
    Lib_StatusSendToSequenceSendToSequence
    ProjectENCORE2ENCORE2
    ID42704271




    Sequencing Information
    BGKcLV42-1BGKcLV42-2
    Sample_IDBGKcLV42-1BGKcLV42-2
    Sample NameNT-BGKcLV42-1NT-BGKcLV42-2
    Pool IDPool-240715Pool-240715
    LocalServer_folderset73set73
    total_reads52,379,59138,117,608
    total_aligned_reads45,702,86232,002,726
    unique_aligned_reads42,005,46329,473,485
    percent_uniqueAligned0.801940.77322
    correlation_replicates0.9978550.997855
    spikein_reads4,616848
    percent_spikeins0.000090.00002
    original_ReadLength101101
    QC_StatusSubmittingSubmitting
    ID31373138




    Data Submission Information
    DCC submission information was not avaliable




    File Information
    file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
    NT-BGKcLV42-1_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set73/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3137NovaSeq6000100paired-ended1ENCORE230388
    NT-BGKcLV42-1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set73/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3137NovaSeq6000100paired-ended2ENCORE230478
    NT-BGKcLV42-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3137GRCh38V40NT-BGKcLV42-1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV42-1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231320
    NT-BGKcLV42-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3137GRCh38V40NT-BGKcLV42-1_Aligned.sortedByCoord.out.bamENCORE231410
    NT-BGKcLV42-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3137GRCh38V40NT-BGKcLV42-1_Aligned.sortedByCoord.out.bamENCORE231500
    NT-BGKcLV42-1_Signal.Unique.strand-.bwENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3137GRCh38V40NT-BGKcLV42-1_Aligned.sortedByCoord.out.bamENCORE231590
    NT-BGKcLV42-1_Signal.Unique.strand+.bwENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3137GRCh38V40NT-BGKcLV42-1_Aligned.sortedByCoord.out.bamENCORE231680
    NT-BGKcLV42-1_quant.sfENCODE_DATA/set73/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3137GRCh38V40NT-BGKcLV42-1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV42-1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231770
    NT-BGKcLV42-2_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set73/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3138NovaSeq6000100paired-ended1ENCORE230389
    NT-BGKcLV42-2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set73/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3138NovaSeq6000100paired-ended2ENCORE230479
    NT-BGKcLV42-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3138GRCh38V40NT-BGKcLV42-2_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV42-2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231321
    NT-BGKcLV42-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3138GRCh38V40NT-BGKcLV42-2_Aligned.sortedByCoord.out.bamENCORE231411
    NT-BGKcLV42-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3138GRCh38V40NT-BGKcLV42-2_Aligned.sortedByCoord.out.bamENCORE231501
    NT-BGKcLV42-2_Signal.Unique.strand-.bwENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3138GRCh38V40NT-BGKcLV42-2_Aligned.sortedByCoord.out.bamENCORE231591
    NT-BGKcLV42-2_Signal.Unique.strand+.bwENCODE_DATA/set73/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3138GRCh38V40NT-BGKcLV42-2_Aligned.sortedByCoord.out.bamENCORE231681
    NT-BGKcLV42-2_quant.sfENCODE_DATA/set73/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3138GRCh38V40NT-BGKcLV42-2_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV42-2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231771