AHCYL2

General Information

Gene Name : AHCYL2
Gene ID : ENSG00000158467.16
Essential Gene : No

Functions

Novel RBP

Localization

Not Available

Domains (AlphaFold: AHCYL2)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A300-262A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	AHCYL2	ENSG00000158467.16	6.16	5.26	5.71	ENCSR000AEF
HepG2	AHCYL2	ENSG00000158467.16	6.35	6.53	6.44	ENCSR985KAT
K562	AHCYL2	ENSG00000158467.16	3.45	4.15	3.80	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	AHCYL2-BGKcLV26-K562	ENCSR700JXJ	AHCYL2	K562	Released		ENCSR900GQL	CRISPR	KD% = 78.1,85.3
knockdown followed by RNA-seq	AHCYL2-BGHcLV25-HepG2	ENCSR363NMO	AHCYL2	HepG2	Released		ENCSR953VMB	CRISPR	KD% = 70.2,67.8

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
AHCYL2	ENCAB809CIX	A300-262A	2			Released, ENCODE4	BGHcLV25-21, Released, ENCODE4	BGKcLV26-17, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
AHCYL2	K562	CRISPR	ENCSR700JXJ	ENCSR900GQL	AHCYL2-BGKcLV26-17,AHCYL2-BGKcLV26-18	NT-BGKcLV26-1,NT-BGKcLV26-2	78.1,85.3	Released	ENCODE4
AHCYL2	HepG2	CRISPR	ENCSR363NMO	ENCSR953VMB	AHCYL2-BGHcLV25-21,AHCYL2-BGHcLV25-22	NT-BGHcLV25-1,NT-BGHcLV25-2	70.2,67.8	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
AHCYL2	Product_ID: A300-262A Lot_ID: 2 Source: Bethyl Labs Target Name: AHCYL2-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for AHCYL2 is not avaliable on ENCORE database.

Proteins bound to RNA

AHCYL2 RNA is bound by 14 proteins.

CanvasJS.com

eCLIP assay for AHCYL2 is not avaliable on ENCORE database.

14 proteins are bound to AHCYL2 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr7	128898963	128899030	+	intron-Distal	4.66329	12.19689	HepG2	QKI
chr7	128864863	128864980	+	5'-UTR,CDS	5.67603	11.61399	K562	GTF2F1
chr7	128873893	128873941	+	intron-Distal	4.84813	9.53330	K562	PTBP1
chr7	128873794	128873933	+	intron-Distal	3.57194	8.57718	HepG2	PTBP1
chr7	128951477	128951584	+	intron-Distal	5.41454	7.97897	HepG2	QKI
chr7	128873902	128873956	+	intron-Distal	4.70846	6.58366	HepG2	SUGP2
chr7	128914276	128914333	+	intron-Distal	5.06230	6.37401	HepG2	RBFOX2
chr7	128980665	128980825	+	intron-Distal	4.64929	6.09994	K562	KHSRP
chr7	128875102	128875144	+	intron-Distal	4.68721	6.08205	HepG2	MATR3
chr7	129043233	129043327	+	CDS,intron-Proximal	4.92259	6.04243	HepG2	BCLAF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 28 entries

eCLIP assay for AHCYL2 is not avaliable on ENCORE database.

Summary
DE Affected by

Diffrential Expression

The Knockdown/knockout assay for AHCYL2 is not available on ENCORE database.
65 RBPs can affect the levels of AHCYL2.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for AHCYL2 is not available on ENCORE database.

65 RBPs can affect the gene expression of AHCYL2 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
AHCYL2	ENSG00000158467.12	291.83750	647.49060	-1.14970	23.54830	22.34330	HepG2	ZNF622
AHCYL2	ENSG00000158467.12	286.65940	618.97010	-1.11050	20.62640	19.31030	HepG2	PUM1
AHCYL2	ENSG00000158467.12	388.28160	687.25750	-0.82370	13.01960	11.97940	HepG2	SRSF9
AHCYL2	ENSG00000158467.12	320.90430	714.46840	-1.15470	12.19850	11.00920	HepG2	DDX6
AHCYL2	ENSG00000158467.12	727.64270	416.55580	0.80460	12.19180	10.93060	K562	DDX47
AHCYL2	ENSG00000158467.12	577.88230	228.75090	1.33690	12.16660	10.43160	K562	RPS19
AHCYL2	ENSG00000158467.12	770.13110	488.45630	0.65680	9.73560	8.69870	HepG2	PUS1
AHCYL2	ENSG00000158467.12	276.58250	479.54830	-0.79400	9.55550	8.56470	HepG2	ACO1
AHCYL2	ENSG00000158467.12	445.90380	868.32700	-0.96150	9.35190	8.43750	HepG2	AKAP1
AHCYL2	ENSG00000158467.12	580.35050	345.89640	0.74650	9.26420	8.04360	K562	UPF1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 72 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

AHCYL2

General Information

Functions

Localization

Domains (AlphaFold: AHCYL2)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for AHCYL2 is not avaliable on ENCORE database.

14 proteins are bound to AHCYL2 RNA:

eCLIP assay for AHCYL2 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for AHCYL2 is not available on ENCORE database.

65 RBPs can affect the gene expression of AHCYL2 :

The Knockdown/knockout RNA-Seq assay for AHCYL2 is not available on ENCORE database.

No RBPs affect the alternative splcing events of AHCYL2 on ENCORE database.

The Knockdown/knockout RNA-Seq assay for AHCYL2 is not avaliable on ENCORE database.